Lampalizumab / 4d9r

A4DAB150_4d9r_H_L | Source PDB: 4d9r | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6846
Max Score
1.1816
Min Score
-3.6466
Total Score
-295.75
Score CDR
-0.6642
Score non-CDR
-0.6868

Calculation Parameters

Chain Sequence(s) EVQLVQSGPELKKPGASVKVSCKASGYTFTNYGMNWVRQAPGQGLEWMGWINTYTGETTYADDFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCEREGGVNNWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQVTQSPSSLSASVGDRVTITCITSTDIDDDMNWYQQKPGKVPKLLISGGNTLRPGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCLQSDSLPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6466
Maximum Score 1.1816
Average Score -0.6846
Total Score -295.7508

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0806 Soluble
2 V H -1.0888 Soluble
3 Q H -1.4956 Soluble
4 L H 0.0000 Buried
5 V H 0.1990 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.5749 Soluble
8 G H -0.7829 Soluble
9 P H -0.8977 Soluble
11 E H -1.5405 Soluble
12 L H -0.9487 Soluble
13 K H -1.7154 Soluble
14 K H -2.3876 Soluble
15 P H -2.0497 Soluble
16 G H -1.5038 Soluble
17 A H -1.2307 Soluble
18 S H -1.3310 Soluble
19 V H 0.0000 Buried
20 K H -1.8918 Soluble
21 V H 0.0000 Buried
22 S H -0.6669 Soluble
23 C H 0.0000 Buried
24 K H -0.8421 Soluble
25 A H 0.0000 Buried
26 S H -0.7746 Soluble
27 G H -1.2185 Soluble
28 Y H -0.6496 Soluble
29 T H -0.4059 Soluble
30 F H 0.0000 Buried
35 T H -0.1414 Soluble
36 N H -0.9525 Soluble
37 Y H -0.3728 Soluble
38 G H 0.0000 Buried
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.6694 Soluble
45 A H 0.0000 Buried
46 P H -1.0117 Soluble
47 G H -1.2459 Soluble
48 Q H -1.8605 Soluble
49 G H -1.3909 Soluble
50 L H 0.0000 Buried
51 E H -1.2904 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 W H -0.0660 Soluble
56 I H 0.0000 Buried
57 N H -0.6545 Soluble
58 T H 0.0000 Buried
59 Y H 0.6331 Aggregation-prone
62 T H -0.2381 Soluble
63 G H -0.9379 Soluble
64 E H -1.8515 Soluble
65 T H -0.7366 Soluble
66 T H -0.5120 Soluble
67 Y H -0.9393 Soluble
68 A H -1.9491 Soluble
69 D H -3.5414 Soluble
70 D H -3.2404 Soluble
71 F H 0.0000 Buried
72 K H -2.8482 Soluble
74 G H -1.7308 Soluble
75 R H -1.0208 Soluble
76 F H 0.0000 Buried
77 V H 0.3072 Aggregation-prone
78 F H 0.0000 Buried
79 S H -0.2944 Soluble
80 L H 0.1804 Aggregation-prone
81 D H -0.4318 Soluble
82 T H 0.1853 Aggregation-prone
83 S H 0.3335 Aggregation-prone
84 V H 1.0901 Aggregation-prone
85 S H 0.1389 Aggregation-prone
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.4312 Soluble
89 L H 0.0000 Buried
90 Q H -0.8322 Soluble
91 I H 0.0000 Buried
92 S H -0.9359 Soluble
93 S H -1.1332 Soluble
94 L H 0.0000 Buried
95 K H -2.3246 Soluble
96 A H -1.7944 Soluble
97 E H -2.2149 Soluble
98 D H 0.0000 Buried
99 T H -0.8662 Soluble
100 A H 0.0000 Buried
101 V H 0.2359 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 E H 0.0000 Buried
106 R H 0.0000 Buried
107 E H -0.5117 Soluble
108 G H -0.3854 Soluble
114 G H 0.1779 Aggregation-prone
115 V H 1.1816 Aggregation-prone
116 N H -0.0416 Soluble
117 N H -0.3905 Soluble
118 W H -0.3677 Soluble
119 G H 0.0000 Buried
120 Q H -1.0692 Soluble
121 G H -0.3844 Soluble
122 T H 0.0000 Buried
123 L H -0.1602 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.2945 Soluble
128 S H -1.0067 Soluble
129 A H -0.6894 Soluble
130 S H -0.8769 Soluble
131 T H -1.0756 Soluble
132 K H -1.8478 Soluble
133 G H -1.6372 Soluble
134 P H 0.0000 Buried
135 S H -0.3655 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9535 Soluble
139 L H 0.0000 Buried
140 A H -0.9935 Soluble
141 P H 0.0000 Buried
142 S H -0.7905 Soluble
143 S H -0.6016 Soluble
144 K H -0.7590 Soluble
145 S H 0.0000 Buried
146 T H -0.6004 Soluble
147 S H -0.6766 Soluble
148 G H -0.8339 Soluble
149 G H -0.8870 Soluble
150 T H -0.6080 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.5956 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4767 Soluble
164 P H -0.7042 Soluble
165 V H -0.7150 Soluble
166 T H -0.6690 Soluble
167 V H -0.4065 Soluble
168 S H -0.4147 Soluble
169 W H 0.0000 Buried
170 N H -0.7067 Soluble
171 S H -0.6347 Soluble
172 G H -0.5093 Soluble
173 A H -0.2270 Soluble
174 L H -0.0106 Soluble
175 T H -0.1567 Soluble
176 S H -0.1762 Soluble
177 G H -0.1824 Soluble
178 V H 0.2174 Aggregation-prone
179 H H -0.3151 Soluble
180 T H 0.0281 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.4266 Soluble
183 A H 0.3156 Aggregation-prone
184 V H 0.6556 Aggregation-prone
185 L H 1.1104 Aggregation-prone
186 Q H 0.2616 Aggregation-prone
187 S H -0.0956 Soluble
188 S H -0.2023 Soluble
189 G H 0.0811 Aggregation-prone
190 L H 0.0743 Aggregation-prone
191 Y H 0.4066 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1199 Soluble
199 V H 0.0000 Buried
200 P H -0.5101 Soluble
201 S H -0.5586 Soluble
202 S H -0.5518 Soluble
203 S H -0.5743 Soluble
204 L H -0.6224 Soluble
205 G H -0.9299 Soluble
206 T H -0.6995 Soluble
207 Q H -1.1814 Soluble
208 T H -1.0706 Soluble
209 Y H 0.0000 Buried
210 I H -1.1832 Soluble
211 C H 0.0000 Buried
212 N H -1.4535 Soluble
213 V H 0.0000 Buried
214 N H -2.2434 Soluble
215 H H 0.0000 Buried
216 K H -2.9640 Soluble
217 P H -1.9308 Soluble
218 S H -2.0997 Soluble
219 N H -2.6547 Soluble
220 T H -2.1925 Soluble
221 K H -2.7518 Soluble
222 V H -1.6208 Soluble
223 D H -2.2510 Soluble
224 K H -1.9424 Soluble
225 K H -2.3618 Soluble
226 V H 0.0000 Buried
227 E H -2.2528 Soluble
228 P H -1.6898 Soluble
229 K H -2.0210 Soluble
230 S H -0.9115 Soluble
231 C H -0.1051 Soluble
1 D L -2.1842 Soluble
2 I L -1.4119 Soluble
3 Q L -1.4062 Soluble
4 V L -0.6338 Soluble
5 T L -0.5428 Soluble
6 Q L 0.0000 Buried
7 S L -0.3565 Soluble
8 P L -0.5484 Soluble
9 S L -0.7383 Soluble
10 S L -0.5773 Soluble
11 L L -0.3818 Soluble
12 S L -0.8480 Soluble
13 A L -0.9984 Soluble
14 S L -0.8702 Soluble
15 V L 0.1371 Aggregation-prone
16 G L -0.7969 Soluble
17 D L -1.8223 Soluble
18 R L -2.3553 Soluble
19 V L 0.0000 Buried
20 T L -0.6610 Soluble
21 I L 0.0000 Buried
22 T L -0.3853 Soluble
23 C L 0.0000 Buried
24 I L -0.4810 Soluble
25 T L 0.0000 Buried
26 S L -1.1554 Soluble
27 T L -1.3788 Soluble
28 D L -2.8938 Soluble
29 I L 0.0000 Buried
36 D L -3.5184 Soluble
37 D L -2.8682 Soluble
38 D L -2.4487 Soluble
39 M L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6406 Soluble
46 P L -1.6367 Soluble
47 G L -1.6080 Soluble
48 K L -2.0855 Soluble
49 V L -0.8399 Soluble
50 P L 0.0000 Buried
51 K L -1.1062 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 S L -0.4684 Soluble
56 G L -1.3176 Soluble
57 G L -1.3037 Soluble
65 N L -0.8146 Soluble
66 T L -0.2089 Soluble
67 L L 0.2440 Aggregation-prone
68 R L -0.2075 Soluble
69 P L -0.2845 Soluble
70 G L -0.6258 Soluble
71 V L 0.0000 Buried
72 P L -0.3903 Soluble
74 S L -0.3943 Soluble
75 R L -0.6840 Soluble
76 F L 0.0000 Buried
77 S L -0.2563 Soluble
78 G L -0.3460 Soluble
79 S L -0.9570 Soluble
80 G L -1.5145 Soluble
83 S L -2.2702 Soluble
84 G L -2.5192 Soluble
85 T L -1.9968 Soluble
86 D L -2.1776 Soluble
87 F L 0.0000 Buried
88 T L -0.5934 Soluble
89 L L 0.0000 Buried
90 T L -0.6297 Soluble
91 I L 0.0000 Buried
92 S L -1.3556 Soluble
93 S L -1.1266 Soluble
94 L L 0.0000 Buried
95 Q L -0.8532 Soluble
96 P L -1.5792 Soluble
97 E L -2.0676 Soluble
98 D L 0.0000 Buried
99 V L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.7105 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 S L -0.8241 Soluble
108 D L -1.7145 Soluble
109 S L -0.4850 Soluble
114 L L 0.3209 Aggregation-prone
115 P L -0.2329 Soluble
116 Y L -0.2297 Soluble
117 T L -0.6932 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.6329 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.1198 Soluble
124 V L 0.0000 Buried
125 E L -1.0781 Soluble
126 I L 0.0000 Buried
127 K L -1.2425 Soluble
128 R L -0.8216 Soluble
129 T L -0.0914 Soluble
130 V L 0.4367 Aggregation-prone
131 A L 0.1232 Aggregation-prone
132 A L -0.0660 Soluble
133 P L 0.0000 Buried
134 S L -0.0940 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6821 Soluble
140 P L -0.7768 Soluble
141 S L -1.6687 Soluble
142 D L -2.7573 Soluble
143 E L -2.6724 Soluble
144 Q L 0.0000 Buried
145 L L -2.1803 Soluble
146 K L -2.7109 Soluble
147 S L -1.6834 Soluble
148 G L -1.2226 Soluble
149 T L -0.9518 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7149 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3040 Soluble
162 R L -1.9777 Soluble
163 E L -2.7354 Soluble
164 A L -2.0473 Soluble
165 K L -2.4234 Soluble
166 V L 0.0000 Buried
167 Q L -0.8016 Soluble
168 W L 0.0000 Buried
169 K L -0.5714 Soluble
170 V L 0.0000 Buried
171 D L -1.9199 Soluble
172 N L -1.5371 Soluble
173 A L -0.2783 Soluble
174 L L 0.6832 Aggregation-prone
175 Q L -0.1246 Soluble
176 S L -0.4269 Soluble
177 G L -0.8590 Soluble
178 N L -0.7564 Soluble
179 S L -1.0249 Soluble
180 Q L -1.1673 Soluble
181 E L -1.6968 Soluble
182 S L -0.5371 Soluble
183 V L -0.6229 Soluble
184 T L -1.0468 Soluble
185 E L -2.2807 Soluble
186 Q L 0.0000 Buried
187 D L -2.3738 Soluble
188 S L -2.4036 Soluble
189 K L -2.6563 Soluble
190 D L -1.9087 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5726 Soluble
199 L L 0.0000 Buried
200 T L -0.3933 Soluble
201 L L -0.5973 Soluble
202 S L -1.1389 Soluble
203 K L -2.0208 Soluble
204 A L -1.8174 Soluble
205 D L -2.3684 Soluble
206 Y L 0.0000 Buried
207 E L -3.5589 Soluble
208 K L -3.6466 Soluble
209 H L -2.9700 Soluble
210 K L -3.0040 Soluble
211 V L -1.4021 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7292 Soluble
216 V L 0.0000 Buried
217 T L -1.1950 Soluble
218 H L 0.0000 Buried
219 Q L -1.6237 Soluble
220 G L -0.3886 Soluble
221 L L -0.1747 Soluble
222 S L -0.4301 Soluble
223 S L -0.3591 Soluble
224 P L -0.4547 Soluble
225 V L 0.3390 Aggregation-prone
226 T L -0.2203 Soluble
227 K L -0.4446 Soluble
228 S L -0.4995 Soluble
229 F L 0.0000 Buried
230 N L -1.5813 Soluble
231 R L -2.4078 Soluble
232 G L -1.5794 Soluble
233 E L -1.4082 Soluble
234 C L -0.7807 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6204 1.8717 CSV PDB
4.5 -0.6744 1.8717 CSV PDB
5.0 -0.7371 1.8717 CSV PDB
5.5 -0.7966 1.8717 CSV PDB
6.0 -0.841 1.8717 CSV PDB
6.5 -0.8606 1.8717 CSV PDB
7.0 -0.8556 1.8717 CSV PDB
7.5 -0.8344 1.8717 CSV PDB
8.0 -0.8044 1.8717 CSV PDB
8.5 -0.7671 1.8717 CSV PDB
9.0 -0.7213 1.8717 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH