Amatuximab / 7ued

A4DAB011_7ued_H_L | Source PDB: 7ued | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6686
Max Score
1.26
Min Score
-3.5636
Total Score
-288.15
Score CDR
-0.3133
Score non-CDR
-0.71

Calculation Parameters

Chain Sequence(s) VQLQQSGPELEKPGASVKISCKASGYSFTGYTMNWVKQSHGKSLEWIGLITPYNGASSYNQKFRGKATLTVDKSSSTAYMDLLSLTSEDSAVYFCARGGYDGRGFDYWGSGTPVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIELTQSPAIMSASPGEKVTMTCSASSSVSYMHWYQQKSGTSPKRWIYDTSKLASGVPGRFSGSGSGNSYSLTISSVEAEDDATYYCQQWSKHPLTFGSGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5636
Maximum Score 1.26
Average Score -0.6686
Total Score -288.1471

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 1.0338 Aggregation-prone
3 Q H -0.7248 Soluble
4 L H 0.0000 Buried
5 Q H -1.4341 Soluble
6 Q H -1.0191 Soluble
7 S H -0.8155 Soluble
8 G H -0.7478 Soluble
9 P H -0.6949 Soluble
11 E H -0.9542 Soluble
12 L H -0.7424 Soluble
13 E H -1.5807 Soluble
14 K H -2.2846 Soluble
15 P H -1.4315 Soluble
16 G H -1.1017 Soluble
17 A H -0.8911 Soluble
18 S H -0.8757 Soluble
19 V H -1.0830 Soluble
20 K H -1.7654 Soluble
21 I H 0.0000 Buried
22 S H -0.7857 Soluble
23 C H 0.0000 Buried
24 K H -1.4573 Soluble
25 A H 0.0000 Buried
26 S H -0.5168 Soluble
27 G H -0.0298 Soluble
28 Y H 0.0882 Aggregation-prone
29 S H -0.4220 Soluble
30 F H 0.0000 Buried
35 T H -0.1814 Soluble
36 G H 0.0218 Aggregation-prone
37 Y H 0.4106 Aggregation-prone
38 T H 0.4239 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 K H -0.7157 Soluble
44 Q H -1.1074 Soluble
45 S H -1.6375 Soluble
46 H H -1.7974 Soluble
47 G H -1.8413 Soluble
48 K H -2.4422 Soluble
49 S H -1.4136 Soluble
50 L H 0.0000 Buried
51 E H -1.1155 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 L H 0.3120 Aggregation-prone
56 I H 0.0000 Buried
57 T H 0.0263 Aggregation-prone
58 P H 0.0000 Buried
59 Y H 0.3667 Aggregation-prone
62 N H -0.7647 Soluble
63 G H -0.6419 Soluble
64 A H -0.2710 Soluble
65 S H -0.1996 Soluble
66 S H -0.3023 Soluble
67 Y H -0.8663 Soluble
68 N H 0.0000 Buried
69 Q H -2.1939 Soluble
70 K H -2.6602 Soluble
71 F H 0.0000 Buried
72 R H -2.8083 Soluble
74 G H -1.7384 Soluble
75 K H -1.3936 Soluble
76 A H 0.0000 Buried
77 T H -0.7496 Soluble
78 L H 0.0000 Buried
79 T H -0.2660 Soluble
80 V H -0.5939 Soluble
81 D H -1.4417 Soluble
82 K H -1.6449 Soluble
83 S H -1.1253 Soluble
84 S H -0.9607 Soluble
85 S H -1.0854 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.4791 Soluble
89 M H 0.0000 Buried
90 D H -1.0658 Soluble
91 L H 0.0000 Buried
92 L H -0.2848 Soluble
93 S H -0.5338 Soluble
94 L H 0.0000 Buried
95 T H -1.2420 Soluble
96 S H -1.5214 Soluble
97 E H -1.9573 Soluble
98 D H 0.0000 Buried
99 S H -0.9445 Soluble
100 A H 0.0000 Buried
101 V H -0.1139 Soluble
102 Y H 0.0000 Buried
103 F H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 G H 0.0000 Buried
108 G H -0.2160 Soluble
109 Y H 0.1444 Aggregation-prone
110 D H -1.4772 Soluble
112 G H -0.9068 Soluble
113 R H -1.2713 Soluble
114 G H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.1537 Soluble
117 Y H 0.5419 Aggregation-prone
118 W H 0.0466 Aggregation-prone
119 G H 0.0000 Buried
120 S H -0.5630 Soluble
121 G H -0.3727 Soluble
122 T H 0.0000 Buried
123 P H -0.2446 Soluble
124 V H 0.0000 Buried
125 T H -0.8169 Soluble
126 V H 0.0000 Buried
127 S H -1.0279 Soluble
128 S H -1.0104 Soluble
129 A H -0.6656 Soluble
130 S H -0.7435 Soluble
131 T H -0.6923 Soluble
132 K H -1.1845 Soluble
133 G H -1.3393 Soluble
134 P H -0.5630 Soluble
135 S H -0.3655 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9979 Soluble
139 L H 0.0000 Buried
140 A H -1.1751 Soluble
141 P H 0.0000 Buried
142 S H -1.1720 Soluble
143 S H -0.9001 Soluble
144 K H -0.9682 Soluble
145 S H 0.0000 Buried
146 T H -0.7350 Soluble
147 S H -0.7392 Soluble
148 G H -0.8328 Soluble
149 G H -0.9004 Soluble
150 T H -0.6338 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.3224 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.5728 Soluble
164 P H -0.6665 Soluble
165 V H -0.4218 Soluble
166 T H -0.6109 Soluble
167 V H -0.1813 Soluble
168 S H -0.3669 Soluble
169 W H 0.0000 Buried
170 N H -0.6793 Soluble
171 S H -0.6104 Soluble
172 G H -0.4408 Soluble
173 A H -0.1811 Soluble
174 L H 0.0400 Aggregation-prone
175 T H -0.1278 Soluble
176 S H -0.3527 Soluble
177 G H -0.3834 Soluble
178 V H 0.2019 Aggregation-prone
179 H H 0.0000 Buried
180 T H 0.0989 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.2288 Soluble
183 A H 0.2452 Aggregation-prone
184 V H 0.5299 Aggregation-prone
185 L H 1.0315 Aggregation-prone
186 Q H 0.2009 Aggregation-prone
187 S H -0.1347 Soluble
188 S H -0.2549 Soluble
189 G H -0.0333 Soluble
190 L H 0.1028 Aggregation-prone
191 Y H 0.4230 Aggregation-prone
192 S H 0.2652 Aggregation-prone
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1023 Soluble
199 V H 0.0000 Buried
200 P H -0.6074 Soluble
201 S H -0.6154 Soluble
202 S H -0.5852 Soluble
203 S H -0.5565 Soluble
204 L H -0.7387 Soluble
205 G H -0.9184 Soluble
206 T H -0.6557 Soluble
207 Q H -1.0692 Soluble
208 T H -0.9600 Soluble
209 Y H 0.0000 Buried
210 I H -1.0416 Soluble
211 C H 0.0000 Buried
212 N H -1.4751 Soluble
213 V H 0.0000 Buried
214 N H -2.0521 Soluble
215 H H 0.0000 Buried
216 K H -2.8282 Soluble
217 P H -1.6531 Soluble
218 S H -1.8395 Soluble
219 N H -2.5969 Soluble
220 T H -2.1120 Soluble
221 K H -2.7726 Soluble
222 V H -1.5497 Soluble
223 D H -2.4292 Soluble
224 K H -1.9563 Soluble
225 K H -2.1506 Soluble
226 V H 0.0000 Buried
227 E H -2.6808 Soluble
228 P H -1.8715 Soluble
229 K H -2.2148 Soluble
1 D L -2.7067 Soluble
2 I L 0.0000 Buried
3 E L -2.4241 Soluble
4 L L 0.0000 Buried
5 T L -0.7174 Soluble
6 Q L 0.0000 Buried
7 S L -0.1238 Soluble
8 P L 0.1958 Aggregation-prone
9 A L 0.4517 Aggregation-prone
10 I L 1.2600 Aggregation-prone
11 M L 0.3264 Aggregation-prone
12 S L -0.3942 Soluble
13 A L 0.0000 Buried
14 S L -1.7521 Soluble
15 P L -2.0531 Soluble
16 G L -1.9365 Soluble
17 E L -2.6406 Soluble
18 K L -2.7422 Soluble
19 V L 0.0000 Buried
20 T L -0.4789 Soluble
21 M L 0.0000 Buried
22 T L -0.2575 Soluble
23 C L 0.0000 Buried
24 S L -0.9737 Soluble
25 A L 0.0000 Buried
26 S L -1.3797 Soluble
27 S L -1.2746 Soluble
28 S L -0.8217 Soluble
29 V L 0.0000 Buried
37 S L -0.0988 Soluble
38 Y L 0.6904 Aggregation-prone
39 M L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.1811 Soluble
46 S L -1.1164 Soluble
47 G L -0.9344 Soluble
48 T L -0.6392 Soluble
49 S L -0.4006 Soluble
50 P L 0.0000 Buried
51 K L -0.7570 Soluble
52 R L -0.5638 Soluble
53 W L -0.4531 Soluble
54 I L 0.0000 Buried
55 Y L -0.6955 Soluble
56 D L -0.8728 Soluble
57 T L -0.5169 Soluble
65 S L -1.0099 Soluble
66 K L -1.8130 Soluble
67 L L -0.7488 Soluble
68 A L 0.0000 Buried
69 S L -0.5557 Soluble
70 G L -0.5110 Soluble
71 V L -0.4019 Soluble
72 P L -0.4098 Soluble
74 G L -0.6923 Soluble
75 R L -1.0445 Soluble
76 F L 0.0000 Buried
77 S L -0.6776 Soluble
78 G L 0.0000 Buried
79 S L -0.6426 Soluble
80 G L -0.5564 Soluble
83 S L -0.6113 Soluble
84 G L -0.8169 Soluble
85 N L -1.3635 Soluble
86 S L -0.9596 Soluble
87 Y L 0.0000 Buried
88 S L -0.3442 Soluble
89 L L 0.0000 Buried
90 T L -0.5823 Soluble
91 I L 0.0000 Buried
92 S L -1.6302 Soluble
93 S L -1.9167 Soluble
94 V L 0.0000 Buried
95 E L -2.4515 Soluble
96 A L -2.4162 Soluble
97 E L -2.6763 Soluble
98 D L 0.0000 Buried
99 D L -1.6302 Soluble
100 A L 0.0000 Buried
101 T L -0.5410 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 W L -0.0965 Soluble
108 S L -0.5572 Soluble
109 K L -1.9607 Soluble
114 H L -1.9070 Soluble
115 P L 0.0000 Buried
116 L L 0.0000 Buried
117 T L -1.1593 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 S L -0.9562 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.7083 Soluble
124 V L 0.0000 Buried
125 E L -0.9912 Soluble
126 I L 0.0000 Buried
127 K L -1.4893 Soluble
128 R L -1.0421 Soluble
129 T L -0.0278 Soluble
130 V L 0.6122 Aggregation-prone
131 A L 0.2393 Aggregation-prone
132 A L -0.0594 Soluble
133 P L -0.1034 Soluble
134 S L -0.1856 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6116 Soluble
140 P L -0.7626 Soluble
141 S L -1.4966 Soluble
142 D L -2.7661 Soluble
143 E L -2.5803 Soluble
144 Q L 0.0000 Buried
145 L L -2.0591 Soluble
146 K L -2.7058 Soluble
147 S L -1.6956 Soluble
148 G L -1.2931 Soluble
149 T L -1.0772 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7194 Soluble
159 F L 0.0000 Buried
160 Y L -0.7824 Soluble
161 P L -1.2299 Soluble
162 R L -2.2530 Soluble
163 E L -2.7579 Soluble
164 A L -2.0772 Soluble
165 K L -2.5975 Soluble
166 V L -1.2664 Soluble
167 Q L -0.9353 Soluble
168 W L 0.0000 Buried
169 K L -0.7048 Soluble
170 V L 0.0000 Buried
171 D L -1.7428 Soluble
172 N L -1.3671 Soluble
173 A L -0.2835 Soluble
174 L L 0.6890 Aggregation-prone
175 Q L -0.1395 Soluble
176 S L -0.4967 Soluble
177 G L -0.8569 Soluble
178 N L -0.7384 Soluble
179 S L -1.0125 Soluble
180 Q L -1.1136 Soluble
181 E L -1.6225 Soluble
182 S L -0.5114 Soluble
183 V L -0.5630 Soluble
184 T L -0.9379 Soluble
185 E L -2.2018 Soluble
186 Q L -1.8388 Soluble
187 D L -2.1215 Soluble
188 S L -2.2195 Soluble
189 K L -2.6395 Soluble
190 D L -1.8844 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5146 Soluble
199 L L 0.0000 Buried
200 T L -0.3828 Soluble
201 L L -0.5945 Soluble
202 S L -1.1528 Soluble
203 K L -1.9882 Soluble
204 A L -1.8662 Soluble
205 D L -2.4533 Soluble
206 Y L 0.0000 Buried
207 E L -3.5354 Soluble
208 K L -3.5636 Soluble
209 H L -3.0286 Soluble
210 K L -3.1710 Soluble
211 V L -1.4738 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8946 Soluble
216 V L 0.0000 Buried
217 T L -1.2384 Soluble
218 H L 0.0000 Buried
219 Q L -1.6720 Soluble
220 G L -0.4160 Soluble
221 L L -0.2945 Soluble
222 S L -0.4240 Soluble
223 S L -0.3903 Soluble
224 P L -0.5018 Soluble
225 V L 0.2078 Aggregation-prone
226 T L -0.2782 Soluble
227 K L -0.4194 Soluble
228 S L -0.4524 Soluble
229 F L -0.9635 Soluble
230 N L -1.9087 Soluble
231 R L -2.5751 Soluble
232 G L -2.1578 Soluble
233 E L -2.5151 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8253 1.9082 CSV PDB
4.5 -0.8665 1.9031 CSV PDB
5.0 -0.9148 1.8986 CSV PDB
5.5 -0.9584 1.9002 CSV PDB
6.0 -0.9839 1.9191 CSV PDB
6.5 -0.9807 1.9715 CSV PDB
7.0 -0.9499 2.0628 CSV PDB
7.5 -0.9016 2.1805 CSV PDB
8.0 -0.8443 2.3098 CSV PDB
8.5 -0.7813 2.4431 CSV PDB
9.0 -0.7135 2.5774 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH