Crenezumab / 5vzx

A4DAB074_5vzx_H_L | Source PDB: 5vzx | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7604
Max Score
1.1323
Min Score
-3.5601
Total Score
-328.49
Score CDR
-0.4166
Score non-CDR
-0.7994

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYGMSWVRQAPGKGLELVASINSNGGSTYYPDSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCASGDYWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIVMTQSPLSLPVTPGEPASISCRSSQSLVYSNGDTYLHWYLQKPGQSPQLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCSQSTHVPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5601
Maximum Score 1.1323
Average Score -0.7604
Total Score -328.4863

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0967 Soluble
2 V H -1.2937 Soluble
3 Q H -1.3087 Soluble
4 L H 0.0000 Buried
5 V H 0.5429 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4675 Soluble
8 G H -0.8709 Soluble
9 G H -0.4996 Soluble
11 G H -0.4665 Soluble
12 L H -0.0197 Soluble
13 V H -0.5723 Soluble
14 Q H -1.5244 Soluble
15 P H -1.6908 Soluble
16 G H -1.4772 Soluble
17 G H -1.1877 Soluble
18 S H -1.4834 Soluble
19 L H -1.3566 Soluble
20 R H -2.3467 Soluble
21 L H 0.0000 Buried
22 S H -0.5236 Soluble
23 C H 0.0000 Buried
24 A H -0.2889 Soluble
25 A H 0.0000 Buried
26 S H -1.1064 Soluble
27 G H -1.2205 Soluble
28 F H -0.4025 Soluble
29 T H -0.1182 Soluble
30 F H 0.0000 Buried
35 S H -1.0163 Soluble
36 S H -0.4670 Soluble
37 Y H -0.2748 Soluble
38 G H -0.7754 Soluble
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.9478 Soluble
45 A H -1.1823 Soluble
46 P H -1.0476 Soluble
47 G H -1.4666 Soluble
48 K H -2.4391 Soluble
49 G H -1.6949 Soluble
50 L H -1.1077 Soluble
51 E H -1.5163 Soluble
52 L H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 S H 0.1393 Aggregation-prone
56 I H 0.0000 Buried
57 N H -1.4042 Soluble
58 S H -1.3253 Soluble
59 N H -1.9637 Soluble
62 G H -1.6174 Soluble
63 G H -1.2808 Soluble
64 S H -0.8915 Soluble
65 T H -0.0554 Soluble
66 Y H 0.2634 Aggregation-prone
67 Y H -0.6094 Soluble
68 P H -1.6733 Soluble
69 D H -2.5557 Soluble
70 S H -1.8105 Soluble
71 V H 0.0000 Buried
72 K H -2.6091 Soluble
74 G H -1.8714 Soluble
75 R H -1.8840 Soluble
76 F H 0.0000 Buried
77 T H -1.0346 Soluble
78 I H 0.0000 Buried
79 S H -0.5381 Soluble
80 R H -1.2975 Soluble
81 D H -1.6430 Soluble
82 N H -1.6198 Soluble
83 A H -1.3320 Soluble
84 K H -2.2527 Soluble
85 N H -1.6930 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.7115 Soluble
89 L H 0.0000 Buried
90 Q H -1.7790 Soluble
91 M H 0.0000 Buried
92 N H -2.1106 Soluble
93 S H -1.5090 Soluble
94 L H 0.0000 Buried
95 R H -2.3809 Soluble
96 A H -1.7331 Soluble
97 E H -2.2521 Soluble
98 D H 0.0000 Buried
99 T H -0.7522 Soluble
100 A H 0.0000 Buried
101 V H 0.0412 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H -0.0148 Soluble
107 G H -0.1612 Soluble
116 D H -0.1480 Soluble
117 Y H -0.2058 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.3590 Soluble
121 G H -0.5652 Soluble
122 T H -0.2279 Soluble
123 T H -0.2152 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.9564 Soluble
128 S H -0.6158 Soluble
129 A H -0.5089 Soluble
130 S H -0.6988 Soluble
131 T H -1.0165 Soluble
132 K H -1.9215 Soluble
133 G H -1.8314 Soluble
134 P H 0.0000 Buried
135 S H -0.4308 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1938 Soluble
139 L H 0.0000 Buried
140 A H -1.1263 Soluble
141 P H 0.0000 Buried
142 S H -1.3174 Soluble
143 S H -1.2579 Soluble
144 K H -1.9258 Soluble
145 S H -1.2762 Soluble
146 T H -1.0597 Soluble
147 S H -0.8709 Soluble
148 G H -0.8754 Soluble
149 G H -0.9250 Soluble
150 T H -0.6604 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.8450 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.5571 Soluble
164 P H -0.6266 Soluble
165 V H -0.6025 Soluble
166 T H -0.5446 Soluble
167 V H -0.3421 Soluble
168 S H -0.3608 Soluble
169 W H 0.0000 Buried
170 N H -0.7506 Soluble
171 S H -0.6806 Soluble
172 G H -0.5288 Soluble
173 A H -0.2848 Soluble
174 L H -0.0263 Soluble
175 T H -0.1889 Soluble
176 S H -0.1729 Soluble
177 G H -0.2078 Soluble
178 V H 0.1553 Aggregation-prone
179 H H -0.1713 Soluble
180 T H 0.0111 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3250 Soluble
183 A H 0.1867 Aggregation-prone
184 V H 0.4836 Aggregation-prone
185 L H 0.9870 Aggregation-prone
186 Q H 0.1381 Aggregation-prone
187 S H -0.1949 Soluble
188 S H -0.2973 Soluble
189 G H -0.0447 Soluble
190 L H -0.1386 Soluble
191 Y H 0.2147 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1575 Soluble
199 V H 0.0000 Buried
200 P H -0.5758 Soluble
201 S H -0.8236 Soluble
202 S H -0.5037 Soluble
203 S H -0.4911 Soluble
204 L H -0.7104 Soluble
205 G H -0.9538 Soluble
206 T H -0.6620 Soluble
207 Q H -0.8931 Soluble
208 T H -0.9436 Soluble
209 Y H 0.0000 Buried
210 I H -0.9541 Soluble
211 C H 0.0000 Buried
212 N H -1.3771 Soluble
213 V H 0.0000 Buried
214 N H -1.8612 Soluble
215 H H 0.0000 Buried
216 K H -2.7352 Soluble
217 P H -1.6563 Soluble
218 S H -2.0217 Soluble
219 N H -2.6528 Soluble
220 T H -2.1667 Soluble
221 K H -2.6967 Soluble
222 V H -1.7209 Soluble
223 D H -2.4859 Soluble
224 K H -2.0799 Soluble
225 K H -2.1267 Soluble
226 V H 0.0000 Buried
227 E H -2.6529 Soluble
228 P H -1.9316 Soluble
229 K H -2.4091 Soluble
230 S H -1.5594 Soluble
1 D L -1.2562 Soluble
2 I L 0.0000 Buried
3 V L 0.7169 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.4204 Soluble
6 Q L 0.0000 Buried
7 S L -0.3364 Soluble
8 P L -0.1161 Soluble
9 L L 0.2255 Aggregation-prone
10 S L -0.1555 Soluble
11 L L -0.2826 Soluble
12 P L -0.7215 Soluble
13 V L 0.0000 Buried
14 T L -1.7103 Soluble
15 P L -2.0666 Soluble
16 G L -2.2429 Soluble
17 E L -2.8909 Soluble
18 P L -2.4191 Soluble
19 A L 0.0000 Buried
20 S L -0.9001 Soluble
21 I L 0.0000 Buried
22 S L -0.9469 Soluble
23 C L 0.0000 Buried
24 R L -1.6899 Soluble
25 S L 0.0000 Buried
26 S L -0.7508 Soluble
27 Q L -1.1522 Soluble
28 S L -0.5010 Soluble
29 L L 0.0000 Buried
30 V L 0.6729 Aggregation-prone
31 Y L 0.2209 Aggregation-prone
32 S L -0.5366 Soluble
34 N L -1.4491 Soluble
35 G L -1.0037 Soluble
36 D L -0.9892 Soluble
37 T L -0.1546 Soluble
38 Y L 0.0954 Aggregation-prone
39 L L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 L L 0.0000 Buried
44 Q L -0.9158 Soluble
45 K L -1.4682 Soluble
46 P L -1.3408 Soluble
47 G L -1.5087 Soluble
48 Q L -1.9764 Soluble
49 S L -1.3283 Soluble
50 P L 0.0000 Buried
51 Q L -0.8592 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.2401 Soluble
56 K L -0.7891 Soluble
57 V L -0.6007 Soluble
65 S L -0.8370 Soluble
66 N L -1.1691 Soluble
67 R L -1.7445 Soluble
68 F L -0.6901 Soluble
69 S L -0.6212 Soluble
70 G L -0.7939 Soluble
71 V L -0.8996 Soluble
72 P L -1.2812 Soluble
74 D L -2.4245 Soluble
75 R L -2.3822 Soluble
76 F L 0.0000 Buried
77 S L -1.6222 Soluble
78 G L -1.0771 Soluble
79 S L -1.0603 Soluble
80 G L -1.0599 Soluble
83 S L -0.7704 Soluble
84 G L -0.7056 Soluble
85 T L -1.4223 Soluble
86 D L -2.3359 Soluble
87 F L 0.0000 Buried
88 T L -1.1858 Soluble
89 L L 0.0000 Buried
90 K L -2.2184 Soluble
91 I L 0.0000 Buried
92 S L -2.5807 Soluble
93 R L -3.5601 Soluble
94 V L 0.0000 Buried
95 E L -3.0213 Soluble
96 A L -2.5895 Soluble
97 E L -2.5307 Soluble
98 D L 0.0000 Buried
99 V L 0.0000 Buried
100 G L 0.0000 Buried
101 V L -0.3640 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 S L 0.0000 Buried
106 Q L 0.0000 Buried
107 S L 0.1383 Aggregation-prone
108 T L 0.0000 Buried
109 H L 0.0302 Aggregation-prone
114 V L 1.1323 Aggregation-prone
115 P L 0.5496 Aggregation-prone
116 W L 0.1500 Aggregation-prone
117 T L 0.0376 Aggregation-prone
118 F L 0.0196 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.3101 Soluble
121 G L -0.7962 Soluble
122 T L 0.0000 Buried
123 K L -0.7753 Soluble
124 V L 0.0000 Buried
125 E L -1.0543 Soluble
126 I L 0.0000 Buried
127 K L -1.2794 Soluble
128 R L -0.8734 Soluble
129 T L 0.0732 Aggregation-prone
130 V L 0.5658 Aggregation-prone
131 A L 0.0750 Aggregation-prone
132 A L -0.0283 Soluble
133 P L 0.0000 Buried
134 S L -0.2439 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L -0.1309 Soluble
138 F L 0.0000 Buried
139 P L -0.5152 Soluble
140 P L -0.8493 Soluble
141 S L -1.7630 Soluble
142 D L -2.8156 Soluble
143 E L -2.7336 Soluble
144 Q L 0.0000 Buried
145 L L -2.1978 Soluble
146 K L -2.7272 Soluble
147 S L -1.6955 Soluble
148 G L -1.3360 Soluble
149 T L -0.8556 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8428 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6567 Soluble
162 R L -3.0489 Soluble
163 E L -3.2812 Soluble
164 A L -2.3831 Soluble
165 K L -2.4576 Soluble
166 V L -1.1571 Soluble
167 Q L -0.7750 Soluble
168 W L 0.0000 Buried
169 K L -0.7770 Soluble
170 V L 0.0000 Buried
171 D L -1.7171 Soluble
172 N L -1.5977 Soluble
173 A L -0.4732 Soluble
174 L L 0.2149 Aggregation-prone
175 Q L -0.4195 Soluble
176 S L -0.5926 Soluble
177 G L -0.8838 Soluble
178 N L -0.8125 Soluble
179 S L -1.1343 Soluble
180 Q L -1.4362 Soluble
181 E L -1.9506 Soluble
182 S L -0.9589 Soluble
183 V L -0.7381 Soluble
184 T L -0.8383 Soluble
185 E L -1.4699 Soluble
186 Q L 0.0000 Buried
187 D L -1.9297 Soluble
188 S L -2.3259 Soluble
189 K L -2.8192 Soluble
190 D L -2.0880 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6579 Soluble
199 L L 0.0000 Buried
200 T L -0.4130 Soluble
201 L L -0.4527 Soluble
202 S L -1.0394 Soluble
203 K L -2.0151 Soluble
204 A L -1.8105 Soluble
205 D L -2.4178 Soluble
206 Y L 0.0000 Buried
207 E L -3.1782 Soluble
208 K L -3.3333 Soluble
209 H L -2.8072 Soluble
210 K L -2.8447 Soluble
211 V L -1.3709 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8286 Soluble
216 V L 0.0000 Buried
217 T L -1.2264 Soluble
218 H L 0.0000 Buried
219 Q L -1.6981 Soluble
220 G L -0.3457 Soluble
221 L L -0.1763 Soluble
222 S L -0.4401 Soluble
223 S L -0.4292 Soluble
224 P L -0.5569 Soluble
225 V L -0.0984 Soluble
226 T L -0.6198 Soluble
227 K L -0.9556 Soluble
228 S L -0.6599 Soluble
229 F L -0.9278 Soluble
230 N L -1.7541 Soluble
231 R L -2.4173 Soluble
232 G L -2.0754 Soluble
233 E L -2.2954 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7571 1.623 CSV PDB
4.5 -0.8021 1.5727 CSV PDB
5.0 -0.8548 1.5049 CSV PDB
5.5 -0.905 1.4313 CSV PDB
6.0 -0.9405 1.3636 CSV PDB
6.5 -0.9522 1.3118 CSV PDB
7.0 -0.9406 1.2875 CSV PDB
7.5 -0.9133 1.2875 CSV PDB
8.0 -0.8772 1.4169 CSV PDB
8.5 -0.8344 1.5782 CSV PDB
9.0 -0.7847 1.7398 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH