Palverafusp / 5x8l

A4DAB200_5x8l_J_O | Source PDB: 5x8l | Chains: J, O | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6449
Max Score
2.1362
Min Score
-3.4151
Total Score
-271.49
Score CDR
-0.2084
Score non-CDR
-0.6971

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSDSWIHWVRQAPGKGLEWVAWISPYGGSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARRHWPGGFDYWGQGTLVTVSSASTKGPSVFPLTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKDIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYLYHPATFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) J (Heavy), O (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4151
Maximum Score 2.1362
Average Score -0.6449
Total Score -271.4941

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E J -2.1866 Soluble
2 V J -1.3573 Soluble
3 Q J -1.4329 Soluble
4 L J 0.0000 Buried
5 V J 0.7277 Aggregation-prone
6 E J 0.0000 Buried
7 S J -0.3720 Soluble
8 G J -0.7736 Soluble
9 G J -0.3973 Soluble
11 G J -0.2173 Soluble
12 L J -0.0239 Soluble
13 V J -0.5717 Soluble
14 Q J -1.5706 Soluble
15 P J -1.7212 Soluble
16 G J -1.4573 Soluble
17 G J -1.0833 Soluble
18 S J -0.8173 Soluble
19 L J -0.7027 Soluble
20 R J -1.3433 Soluble
21 L J 0.0000 Buried
22 S J -0.2395 Soluble
23 C J 0.0000 Buried
24 A J -0.2119 Soluble
25 A J 0.0000 Buried
26 S J -1.1064 Soluble
27 G J -1.2923 Soluble
28 F J -0.7617 Soluble
29 T J -0.6698 Soluble
30 F J 0.0000 Buried
35 S J -0.6733 Soluble
36 D J -1.5123 Soluble
37 S J 0.0000 Buried
38 W J -0.0591 Soluble
39 I J 0.0000 Buried
40 H J 0.0000 Buried
41 W J 0.0000 Buried
42 V J 0.0000 Buried
43 R J 0.0000 Buried
44 Q J -0.8311 Soluble
45 A J -1.1831 Soluble
46 P J -0.8445 Soluble
47 G J -1.4503 Soluble
48 K J -2.4441 Soluble
49 G J -1.6666 Soluble
50 L J 0.0000 Buried
51 E J -1.5026 Soluble
52 W J 0.0000 Buried
53 V J 0.0000 Buried
54 A J 0.0000 Buried
55 W J 0.4620 Aggregation-prone
56 I J 0.0000 Buried
57 S J -0.0371 Soluble
58 P J 0.0000 Buried
59 Y J 0.4404 Aggregation-prone
62 G J -0.2280 Soluble
63 G J -0.1115 Soluble
64 S J 0.0211 Aggregation-prone
65 T J 0.4468 Aggregation-prone
66 Y J 0.7776 Aggregation-prone
67 Y J -0.3833 Soluble
68 A J -1.3657 Soluble
69 D J -2.3506 Soluble
70 S J -1.7363 Soluble
71 V J 0.0000 Buried
72 K J -2.3939 Soluble
74 G J -1.7356 Soluble
75 R J -1.4038 Soluble
76 F J 0.0000 Buried
77 T J -0.5361 Soluble
78 I J 0.0000 Buried
79 S J -0.2462 Soluble
80 A J -0.5734 Soluble
81 D J -1.1484 Soluble
82 T J -0.9628 Soluble
83 S J -1.3774 Soluble
84 K J -2.2175 Soluble
85 N J -1.5865 Soluble
86 T J 0.0000 Buried
87 A J 0.0000 Buried
88 Y J -0.3492 Soluble
89 L J 0.0000 Buried
90 Q J -0.7309 Soluble
91 M J 0.0000 Buried
92 N J -1.2064 Soluble
93 S J -1.2660 Soluble
94 L J 0.0000 Buried
95 R J -2.4627 Soluble
96 A J -1.8422 Soluble
97 E J -2.3284 Soluble
98 D J 0.0000 Buried
99 T J -0.6747 Soluble
100 A J 0.0000 Buried
101 V J 0.2294 Aggregation-prone
102 Y J 0.0000 Buried
103 Y J 0.0000 Buried
104 C J 0.0000 Buried
105 A J 0.0000 Buried
106 R J -0.1269 Soluble
107 R J 0.1003 Aggregation-prone
108 H J 0.3946 Aggregation-prone
109 W J 0.9532 Aggregation-prone
110 P J 0.5469 Aggregation-prone
113 G J 0.0000 Buried
114 G J 0.0000 Buried
115 F J 0.0000 Buried
116 D J 0.0000 Buried
117 Y J -0.0648 Soluble
118 W J 0.0000 Buried
119 G J 0.0000 Buried
120 Q J -1.4476 Soluble
121 G J -0.6457 Soluble
122 T J 0.0000 Buried
123 L J 0.3234 Aggregation-prone
124 V J 0.0000 Buried
125 T J -0.4197 Soluble
126 V J 0.0000 Buried
127 S J -0.9591 Soluble
128 S J -0.7195 Soluble
129 A J -0.4811 Soluble
130 S J -0.5771 Soluble
131 T J -0.6615 Soluble
132 K J -1.1562 Soluble
133 G J -1.3745 Soluble
134 P J -0.6498 Soluble
135 S J -0.4458 Soluble
136 V J 0.0000 Buried
137 F J -0.8315 Soluble
138 P J -0.5326 Soluble
139 L J -0.0046 Soluble
140 T J -0.4529 Soluble
141 S J -0.6827 Soluble
142 G J -0.8841 Soluble
143 G J -0.8854 Soluble
144 T J -0.5953 Soluble
145 A J 0.0000 Buried
146 A J 0.4986 Aggregation-prone
147 L J 1.0541 Aggregation-prone
148 G J 0.0000 Buried
149 C J 0.0000 Buried
150 L J 0.0000 Buried
151 V J 0.0000 Buried
152 K J 0.0000 Buried
153 D J -0.6356 Soluble
154 Y J 0.0000 Buried
155 F J 0.0000 Buried
156 P J 0.0000 Buried
157 E J -0.6905 Soluble
158 P J -0.8155 Soluble
159 V J -0.7416 Soluble
160 T J -0.5752 Soluble
161 V J -0.2244 Soluble
162 S J -0.3873 Soluble
163 W J 0.0000 Buried
164 N J -0.3032 Soluble
165 S J -0.6659 Soluble
166 G J -0.5976 Soluble
167 A J -0.1265 Soluble
168 L J 0.2231 Aggregation-prone
169 T J -0.1753 Soluble
170 S J -0.1769 Soluble
171 G J -0.1615 Soluble
172 V J 0.2593 Aggregation-prone
173 H J -0.3540 Soluble
174 T J 0.0102 Aggregation-prone
175 F J 0.0000 Buried
176 P J -0.4119 Soluble
177 A J 0.1354 Aggregation-prone
178 V J 0.4135 Aggregation-prone
179 L J 0.8566 Aggregation-prone
180 Q J 0.0586 Aggregation-prone
181 S J -0.1595 Soluble
182 S J -0.2790 Soluble
183 G J -0.0629 Soluble
184 L J -0.0396 Soluble
185 Y J 0.2693 Aggregation-prone
186 S J 0.0000 Buried
187 L J 0.0000 Buried
188 S J 0.0000 Buried
189 S J 0.0000 Buried
190 V J 0.0000 Buried
191 V J 0.0000 Buried
192 T J 0.1285 Aggregation-prone
193 V J 0.1706 Aggregation-prone
194 P J -0.2238 Soluble
195 S J -0.1534 Soluble
196 S J -0.1479 Soluble
197 S J 0.0943 Aggregation-prone
198 L J 0.9473 Aggregation-prone
199 G J -0.0703 Soluble
200 T J -0.2319 Soluble
201 Q J -0.3890 Soluble
202 T J 0.1630 Aggregation-prone
203 Y J 0.7356 Aggregation-prone
204 I J 0.2417 Aggregation-prone
205 C J 0.0000 Buried
206 N J -1.3412 Soluble
207 V J 0.0000 Buried
208 N J -2.1178 Soluble
209 H J 0.0000 Buried
210 K J -2.8608 Soluble
211 P J -1.6779 Soluble
212 S J -1.8990 Soluble
213 N J -2.5246 Soluble
214 T J -2.1637 Soluble
215 K J -2.7804 Soluble
216 V J -1.8569 Soluble
217 D J -2.4938 Soluble
218 K J -1.7504 Soluble
1 D O -2.2106 Soluble
2 I O -1.6361 Soluble
3 Q O -2.1465 Soluble
4 M O 0.0000 Buried
5 T O -1.3378 Soluble
6 Q O 0.0000 Buried
7 S O -0.5969 Soluble
8 P O -0.5539 Soluble
9 S O -0.8161 Soluble
10 S O -0.7802 Soluble
11 L O -0.6768 Soluble
12 S O -1.1464 Soluble
13 A O 0.0000 Buried
14 S O -1.1726 Soluble
15 V O -0.3824 Soluble
16 G O -0.8943 Soluble
17 D O -2.1833 Soluble
18 R O -2.4944 Soluble
19 V O 0.0000 Buried
20 T O -0.6514 Soluble
21 I O 0.0000 Buried
22 T O -0.8192 Soluble
23 C O 0.0000 Buried
24 R O -2.6679 Soluble
25 A O -2.2682 Soluble
26 S O -2.1481 Soluble
27 Q O -2.8895 Soluble
28 D O -2.7217 Soluble
29 V O 0.0000 Buried
36 S O -1.1201 Soluble
37 T O -0.6024 Soluble
38 A O 0.0681 Aggregation-prone
39 V O 0.0000 Buried
40 A O 0.0000 Buried
41 W O 0.0000 Buried
42 Y O 0.0000 Buried
43 Q O 0.0000 Buried
44 Q O 0.0000 Buried
45 K O -1.5356 Soluble
46 P O -1.3665 Soluble
47 G O -1.6377 Soluble
48 K O -2.5500 Soluble
49 A O -1.5246 Soluble
50 P O 0.0000 Buried
51 K O -1.0215 Soluble
52 L O 0.0000 Buried
53 L O 0.0000 Buried
54 I O 0.0000 Buried
55 Y O 0.9147 Aggregation-prone
56 S O 0.6081 Aggregation-prone
57 A O 0.0000 Buried
65 S O 0.7510 Aggregation-prone
66 F O 2.1362 Aggregation-prone
67 L O 1.4575 Aggregation-prone
68 Y O 0.6871 Aggregation-prone
69 S O 0.0771 Aggregation-prone
70 G O -0.3893 Soluble
71 V O -0.1735 Soluble
72 P O -0.2591 Soluble
74 S O -0.3743 Soluble
75 R O -0.7286 Soluble
76 F O 0.0000 Buried
77 S O 0.1239 Aggregation-prone
78 G O 0.1504 Aggregation-prone
79 S O -0.4912 Soluble
80 G O -1.1932 Soluble
83 S O -1.2895 Soluble
84 G O -1.9302 Soluble
85 T O -2.4979 Soluble
86 D O -2.9057 Soluble
87 F O 0.0000 Buried
88 T O -0.8204 Soluble
89 L O 0.0000 Buried
90 T O -0.6070 Soluble
91 I O 0.0000 Buried
92 S O -1.3723 Soluble
93 S O -1.1823 Soluble
94 L O 0.0000 Buried
95 Q O -1.1261 Soluble
96 P O -1.5965 Soluble
97 E O -1.9087 Soluble
98 D O 0.0000 Buried
99 F O -0.8592 Soluble
100 A O 0.0000 Buried
101 T O 0.0000 Buried
102 Y O 0.0000 Buried
103 Y O 0.0000 Buried
104 C O 0.0000 Buried
105 Q O 0.0000 Buried
106 Q O 0.0000 Buried
107 Y O 0.6625 Aggregation-prone
108 L O 0.8982 Aggregation-prone
109 Y O 0.2906 Aggregation-prone
114 H O -0.5652 Soluble
115 P O -0.8623 Soluble
116 A O -0.4030 Soluble
117 T O -0.8592 Soluble
118 F O 0.0000 Buried
119 G O 0.0000 Buried
120 Q O -1.9831 Soluble
121 G O 0.0000 Buried
122 T O 0.0000 Buried
123 K O -1.3823 Soluble
124 V O 0.0000 Buried
125 E O -1.4982 Soluble
126 I O 0.0000 Buried
127 K O -1.9115 Soluble
128 R O -1.0956 Soluble
129 T O -0.0426 Soluble
130 V O 0.6404 Aggregation-prone
131 A O 0.1714 Aggregation-prone
132 A O -0.1217 Soluble
133 P O 0.0000 Buried
134 S O -0.2304 Soluble
135 V O -0.1435 Soluble
136 F O 0.0000 Buried
137 I O 0.0904 Aggregation-prone
138 F O 0.0000 Buried
139 P O -0.5260 Soluble
140 P O -0.5277 Soluble
141 S O -1.4406 Soluble
142 D O -2.7576 Soluble
143 E O -2.8965 Soluble
144 Q O 0.0000 Buried
145 L O -1.5767 Soluble
146 K O -2.4003 Soluble
147 S O -1.5430 Soluble
148 G O -0.7608 Soluble
149 T O -0.3438 Soluble
150 A O 0.0000 Buried
151 S O 0.0000 Buried
152 V O 0.0000 Buried
153 V O 0.0000 Buried
154 C O 0.0000 Buried
155 L O 0.0000 Buried
156 L O 0.0000 Buried
157 N O 0.0000 Buried
158 N O -1.1738 Soluble
159 F O 0.0000 Buried
160 Y O 0.0000 Buried
161 P O -1.8514 Soluble
162 R O -3.2810 Soluble
163 E O -3.2556 Soluble
164 A O -2.3622 Soluble
165 K O -2.3361 Soluble
166 V O -1.1919 Soluble
167 Q O -0.9874 Soluble
168 W O 0.0000 Buried
169 K O -0.9865 Soluble
170 V O -1.0091 Soluble
171 D O -2.1177 Soluble
172 N O -1.7271 Soluble
173 A O -0.4902 Soluble
174 L O 0.7863 Aggregation-prone
175 Q O -0.1735 Soluble
176 S O -0.3477 Soluble
177 G O -0.8442 Soluble
178 N O -0.3920 Soluble
179 S O -1.0851 Soluble
180 Q O -1.4759 Soluble
181 E O -1.9345 Soluble
182 S O -0.9562 Soluble
183 V O -0.7796 Soluble
184 T O -1.1221 Soluble
185 E O -2.2040 Soluble
186 Q O -2.0835 Soluble
187 D O -2.1387 Soluble
188 S O -2.1314 Soluble
189 K O -2.6478 Soluble
190 D O -2.5546 Soluble
191 S O -2.2683 Soluble
192 T O 0.0000 Buried
193 Y O 0.0000 Buried
194 S O 0.0000 Buried
195 L O -0.7997 Soluble
196 S O 0.0000 Buried
197 S O 0.0000 Buried
198 T O -0.7150 Soluble
199 L O 0.0000 Buried
200 T O 0.1634 Aggregation-prone
201 L O 0.5228 Aggregation-prone
202 S O -0.5188 Soluble
203 K O -1.2248 Soluble
204 A O -1.2355 Soluble
205 D O -1.8503 Soluble
206 Y O -1.8495 Soluble
207 E O -3.4151 Soluble
208 K O -3.1758 Soluble
209 H O -2.9550 Soluble
210 K O -2.7305 Soluble
211 V O -1.9735 Soluble
212 Y O 0.0000 Buried
213 A O 0.0000 Buried
214 C O 0.0000 Buried
215 E O -0.9884 Soluble
216 V O 0.0000 Buried
217 T O -1.1579 Soluble
218 H O -1.4818 Soluble
219 Q O -1.5640 Soluble
220 G O -0.4002 Soluble
221 L O -0.2668 Soluble
222 S O -0.4350 Soluble
223 S O -0.4689 Soluble
224 P O -0.4999 Soluble
225 V O -0.2316 Soluble
226 T O -0.5886 Soluble
227 K O -0.8040 Soluble
228 S O -0.5994 Soluble
229 F O -0.9857 Soluble
230 N O -2.3348 Soluble
231 R O -3.3053 Soluble
232 G O -2.5180 Soluble
233 E O -2.6685 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.558 3.9545 CSV PDB
4.5 -0.6075 3.9652 CSV PDB
5.0 -0.6657 3.9806 CSV PDB
5.5 -0.7219 3.9945 CSV PDB
6.0 -0.7651 4.0026 CSV PDB
6.5 -0.7875 4.0061 CSV PDB
7.0 -0.7896 4.0073 CSV PDB
7.5 -0.779 4.0077 CSV PDB
8.0 -0.7614 4.0078 CSV PDB
8.5 -0.7377 4.0079 CSV PDB
9.0 -0.7065 4.0079 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH