Vonlerolizumab / 7yk4

A4DAB300_7yk4_H_L | Source PDB: 7yk4 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7415
Max Score
1.1582
Min Score
-3.8502
Total Score
-317.37
Score CDR
-0.642
Score non-CDR
-0.7529

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGASVKVSCKASGYTFTDSYMSWVRQAPGQGLEWIGDMYPDNGDSSYNQKFRERVTITRDTSTSTAYLELSSLRSEDTAVYYCVLAPRWYFSVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDISNYLNWYQQKPGKAPKLLIYYTSRLRSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGHTLPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8502
Maximum Score 1.1582
Average Score -0.7415
Total Score -317.3742

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 1.1582 Aggregation-prone
3 Q H -0.3151 Soluble
4 L H 0.0000 Buried
5 V H 0.3706 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.5650 Soluble
8 G H -0.8581 Soluble
9 A H 0.0000 Buried
11 E H -1.3391 Soluble
12 V H -0.8624 Soluble
13 K H -1.6294 Soluble
14 K H -2.4074 Soluble
15 P H -1.8176 Soluble
16 G H -1.4503 Soluble
17 A H -1.1728 Soluble
18 S H -1.2555 Soluble
19 V H 0.0000 Buried
20 K H -1.6643 Soluble
21 V H 0.0000 Buried
22 S H -0.4543 Soluble
23 C H 0.0000 Buried
24 K H -0.8571 Soluble
25 A H 0.0000 Buried
26 S H -0.1109 Soluble
27 G H 0.1013 Aggregation-prone
28 Y H 0.1507 Aggregation-prone
29 T H -0.2936 Soluble
30 F H -0.4552 Soluble
35 T H -1.3383 Soluble
36 D H -1.8872 Soluble
37 S H -0.9273 Soluble
38 Y H -0.2735 Soluble
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.5536 Soluble
45 A H -1.0110 Soluble
46 P H -1.0142 Soluble
47 G H -1.3019 Soluble
48 Q H -1.8018 Soluble
49 G H -1.2266 Soluble
50 L H 0.0000 Buried
51 E H -0.5563 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 D H 0.0000 Buried
56 M H 0.0000 Buried
57 Y H -1.2409 Soluble
58 P H 0.0000 Buried
59 D H -2.9992 Soluble
62 N H -2.6646 Soluble
63 G H -2.2744 Soluble
64 D H -2.4610 Soluble
65 S H -1.0424 Soluble
66 S H -0.6937 Soluble
67 Y H -1.0937 Soluble
68 N H -1.7475 Soluble
69 Q H -3.0016 Soluble
70 K H -3.0676 Soluble
71 F H 0.0000 Buried
72 R H -3.5652 Soluble
74 E H -3.1418 Soluble
75 R H 0.0000 Buried
76 V H 0.0000 Buried
77 T H -0.9659 Soluble
78 I H 0.0000 Buried
79 T H -0.7166 Soluble
80 R H -1.2835 Soluble
81 D H -1.5584 Soluble
82 T H -1.1129 Soluble
83 S H -0.6213 Soluble
84 T H -0.7056 Soluble
85 S H -0.7922 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.5822 Soluble
89 L H 0.0000 Buried
90 E H -1.2355 Soluble
91 L H 0.0000 Buried
92 S H -1.0837 Soluble
93 S H -1.1191 Soluble
94 L H 0.0000 Buried
95 R H -2.3479 Soluble
96 S H -1.9598 Soluble
97 E H -2.3016 Soluble
98 D H 0.0000 Buried
99 T H -0.8761 Soluble
100 A H 0.0000 Buried
101 V H 0.1797 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 V H 0.0000 Buried
106 L H 0.0000 Buried
107 A H 0.0000 Buried
108 P H -0.4943 Soluble
109 R H -0.7003 Soluble
113 W H 0.0000 Buried
114 Y H 0.2263 Aggregation-prone
115 F H 0.0000 Buried
116 S H -0.1662 Soluble
117 V H 0.1769 Aggregation-prone
118 W H -0.2551 Soluble
119 G H 0.0000 Buried
120 Q H -1.2918 Soluble
121 G H -0.5859 Soluble
122 T H 0.0000 Buried
123 L H -0.1396 Soluble
124 V H 0.0000 Buried
125 T H -0.9102 Soluble
126 V H 0.0000 Buried
127 S H -1.1631 Soluble
128 S H -1.0573 Soluble
129 A H -0.6672 Soluble
130 S H -0.8160 Soluble
131 T H -1.0599 Soluble
132 K H -1.6061 Soluble
133 G H -1.4726 Soluble
134 P H -0.6653 Soluble
135 S H -0.2801 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1435 Soluble
139 L H 0.0000 Buried
140 A H -0.8301 Soluble
141 P H 0.0000 Buried
142 S H -0.7073 Soluble
143 S H -0.6682 Soluble
144 K H -1.0275 Soluble
145 S H 0.0000 Buried
146 T H -0.6978 Soluble
147 S H -0.7508 Soluble
148 G H -0.8583 Soluble
149 G H -0.9196 Soluble
150 T H -0.6373 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.4521 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.7916 Soluble
164 P H -0.7814 Soluble
165 V H -0.6681 Soluble
166 T H -0.5915 Soluble
167 V H -0.4143 Soluble
168 S H -0.4470 Soluble
169 W H 0.0000 Buried
170 N H -0.8351 Soluble
171 S H -0.6791 Soluble
172 G H -0.5781 Soluble
173 A H -0.2414 Soluble
174 L H -0.0236 Soluble
175 T H -0.2100 Soluble
176 S H -0.2488 Soluble
177 G H -0.3819 Soluble
178 V H 0.1171 Aggregation-prone
179 H H -0.2999 Soluble
180 T H 0.0132 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.2428 Soluble
183 A H 0.1542 Aggregation-prone
184 V H 0.3912 Aggregation-prone
185 L H 0.8645 Aggregation-prone
186 Q H 0.1057 Aggregation-prone
187 S H -0.1641 Soluble
188 S H -0.2892 Soluble
189 G H -0.0755 Soluble
190 L H 0.0150 Aggregation-prone
191 Y H 0.4167 Aggregation-prone
192 S H 0.2459 Aggregation-prone
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1780 Soluble
199 V H 0.0000 Buried
200 P H -0.5264 Soluble
201 S H -0.5618 Soluble
202 S H -0.5305 Soluble
203 S H -0.5542 Soluble
204 L H -0.6725 Soluble
205 G H -0.9798 Soluble
206 T H -0.6883 Soluble
207 Q H -0.9992 Soluble
208 T H -1.1301 Soluble
209 Y H 0.0000 Buried
210 I H -1.3268 Soluble
211 C H 0.0000 Buried
212 N H -1.5542 Soluble
213 V H 0.0000 Buried
214 N H -2.0130 Soluble
215 H H 0.0000 Buried
216 K H -2.7838 Soluble
217 P H 0.0000 Buried
218 S H -1.9792 Soluble
219 N H -2.6067 Soluble
220 T H -2.1272 Soluble
221 K H -2.7231 Soluble
222 V H -1.5190 Soluble
223 D H -2.5820 Soluble
224 K H -2.0136 Soluble
225 K H -2.5861 Soluble
226 V H 0.0000 Buried
227 E H -2.3880 Soluble
228 P H -1.2023 Soluble
1 D L -2.2959 Soluble
2 I L 0.0000 Buried
3 Q L -2.2153 Soluble
4 M L 0.0000 Buried
5 T L -1.1396 Soluble
6 Q L 0.0000 Buried
7 S L -0.5750 Soluble
8 P L -0.4867 Soluble
9 S L -0.8421 Soluble
10 S L -0.9616 Soluble
11 L L -0.6686 Soluble
12 S L -0.8625 Soluble
13 A L -1.2232 Soluble
14 S L -1.3302 Soluble
15 V L -0.6851 Soluble
16 G L -1.4799 Soluble
17 D L -2.8712 Soluble
18 R L -2.8668 Soluble
19 V L 0.0000 Buried
20 T L -0.5182 Soluble
21 I L 0.0000 Buried
22 T L -0.8757 Soluble
23 C L 0.0000 Buried
24 R L -2.5974 Soluble
25 A L -2.2881 Soluble
26 S L -2.0525 Soluble
27 Q L -2.8161 Soluble
28 D L -2.7709 Soluble
29 I L 0.0000 Buried
36 S L -1.1756 Soluble
37 N L -0.5029 Soluble
38 Y L 0.1795 Aggregation-prone
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.4094 Soluble
45 K L -1.7595 Soluble
46 P L -1.3663 Soluble
47 G L -1.6437 Soluble
48 K L -2.5592 Soluble
49 A L -1.5933 Soluble
50 P L 0.0000 Buried
51 K L -1.7087 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.0218 Soluble
56 Y L 0.2498 Aggregation-prone
57 T L 0.0000 Buried
65 S L -0.9562 Soluble
66 R L -1.6055 Soluble
67 L L -0.6029 Soluble
68 R L -1.0305 Soluble
69 S L -0.7434 Soluble
70 G L -0.5594 Soluble
71 V L -0.3403 Soluble
72 P L -0.3144 Soluble
74 S L -0.4182 Soluble
75 R L -0.7586 Soluble
76 F L 0.0000 Buried
77 S L -0.5284 Soluble
78 G L -0.4684 Soluble
79 S L -0.9045 Soluble
80 G L -1.2605 Soluble
83 S L -1.3045 Soluble
84 G L -1.8971 Soluble
85 T L -2.4611 Soluble
86 D L -2.7377 Soluble
87 F L 0.0000 Buried
88 T L -0.8390 Soluble
89 L L 0.0000 Buried
90 T L -0.5764 Soluble
91 I L 0.0000 Buried
92 S L -1.4745 Soluble
93 S L -1.5924 Soluble
94 L L 0.0000 Buried
95 Q L -1.4217 Soluble
96 P L -1.4549 Soluble
97 E L -1.4472 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -1.0493 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 G L 0.0000 Buried
108 H L -0.8129 Soluble
109 T L -0.3522 Soluble
114 L L 0.0458 Aggregation-prone
115 P L 0.0000 Buried
116 P L 0.0000 Buried
117 T L -0.7715 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.5200 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.9071 Soluble
124 V L 0.0000 Buried
125 E L -1.4235 Soluble
126 I L 0.0000 Buried
127 K L -1.3352 Soluble
128 R L -0.7824 Soluble
129 T L -0.0980 Soluble
130 V L 0.4443 Aggregation-prone
131 A L 0.0829 Aggregation-prone
132 A L -0.1396 Soluble
133 P L 0.0000 Buried
134 S L -0.2239 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6772 Soluble
140 P L 0.0000 Buried
141 S L -1.6622 Soluble
142 D L -2.9761 Soluble
143 E L -3.0296 Soluble
144 Q L 0.0000 Buried
145 L L 0.0000 Buried
146 K L -2.9260 Soluble
147 S L -1.8395 Soluble
148 G L -1.4128 Soluble
149 T L -0.9559 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8532 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7808 Soluble
162 R L -3.1478 Soluble
163 E L -3.2391 Soluble
164 A L -2.2921 Soluble
165 K L -2.2202 Soluble
166 V L -0.8717 Soluble
167 Q L -0.4283 Soluble
168 W L 0.0000 Buried
169 K L -0.3929 Soluble
170 V L 0.0000 Buried
171 D L -1.6047 Soluble
172 N L -1.2192 Soluble
173 A L -0.2252 Soluble
174 L L 0.7605 Aggregation-prone
175 Q L -0.1906 Soluble
176 S L -0.4047 Soluble
177 G L -0.9440 Soluble
178 N L -0.8677 Soluble
179 S L -1.1885 Soluble
180 Q L -1.5464 Soluble
181 E L -1.9689 Soluble
182 S L -0.8352 Soluble
183 V L -0.6753 Soluble
184 T L -0.8372 Soluble
185 E L -1.5950 Soluble
186 Q L 0.0000 Buried
187 D L -1.7778 Soluble
188 S L -1.9159 Soluble
189 K L -2.4068 Soluble
190 D L -1.6677 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7471 Soluble
199 L L 0.0000 Buried
200 T L -0.4728 Soluble
201 L L -0.7303 Soluble
202 S L -1.4324 Soluble
203 K L -2.5083 Soluble
204 A L -2.0992 Soluble
205 D L -2.5464 Soluble
206 Y L 0.0000 Buried
207 E L -3.8502 Soluble
208 K L -3.6309 Soluble
209 H L -3.1337 Soluble
210 K L -3.3230 Soluble
211 V L -1.7894 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7958 Soluble
216 V L 0.0000 Buried
217 T L -1.1064 Soluble
218 H L 0.0000 Buried
219 Q L -1.7800 Soluble
220 G L -0.4959 Soluble
221 L L -0.2924 Soluble
222 S L -0.5032 Soluble
223 S L -0.4188 Soluble
224 P L -0.5859 Soluble
225 V L -0.0606 Soluble
226 T L -0.4868 Soluble
227 K L -0.9036 Soluble
228 S L -0.9096 Soluble
229 F L 0.0000 Buried
230 N L -2.3268 Soluble
231 R L -2.4701 Soluble
232 G L -1.7037 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8172 1.4872 CSV PDB
4.5 -0.8651 1.4474 CSV PDB
5.0 -0.9195 1.4 CSV PDB
5.5 -0.9707 1.3761 CSV PDB
6.0 -1.0081 1.3761 CSV PDB
6.5 -1.0236 1.3761 CSV PDB
7.0 -1.0166 1.3761 CSV PDB
7.5 -0.9946 1.3761 CSV PDB
8.0 -0.964 1.3761 CSV PDB
8.5 -0.9262 1.3761 CSV PDB
9.0 -0.8805 1.3761 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH