Glofitamab / 3pp4

A4DAB132_3pp4_H_L | Source PDB: 3pp4 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7056
Max Score
1.326
Min Score
-3.4867
Total Score
-306.25
Score CDR
—
Score non-CDR
—

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGSSVKVSCKASGYAFSYSWINWVRQAPGQGLEWMGRIFPGDGDTDYNGKFKGRVTITADKSTSTAYMELSSLRSEDTAVYYCARNVFDGYWLVYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIVMTQTPLSLPVTPGEPASISCRSSKSLLHSNGITYLYWYLQKPGQSPQLLIYQMSNLVSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCAQNLELPYTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4867
Maximum Score 1.326
Average Score -0.7056
Total Score -306.2485

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.5099 Soluble
2 V H -0.9560 Soluble
3 Q H -1.2462 Soluble
4 L H 0.0000 Buried
5 V H 0.2091 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.5833 Soluble
8 G H -0.4720 Soluble
9 A H -0.3143 Soluble
11 E H -0.8214 Soluble
12 V H -0.4682 Soluble
13 K H -1.4100 Soluble
14 K H -2.2491 Soluble
15 P H -1.7800 Soluble
16 G H -1.5171 Soluble
17 S H -1.1983 Soluble
18 S H -1.3360 Soluble
19 V H 0.0000 Buried
20 K H -2.1068 Soluble
21 V H 0.0000 Buried
22 S H -0.6184 Soluble
23 C H 0.0000 Buried
24 K H -0.9872 Soluble
25 A H 0.0000 Buried
26 S H -0.8946 Soluble
27 G H -1.0383 Soluble
28 Y H -0.1685 Soluble
29 A H 0.4049 Aggregation-prone
30 F H 0.0000 Buried
35 S H -0.0922 Soluble
36 Y H 1.3260 Aggregation-prone
37 S H 0.0000 Buried
38 W H 0.3508 Aggregation-prone
39 I H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.3877 Soluble
44 Q H -0.5840 Soluble
45 A H 0.0000 Buried
46 P H -1.1563 Soluble
47 G H -1.2819 Soluble
48 Q H -1.7978 Soluble
49 G H -1.1482 Soluble
50 L H 0.0000 Buried
51 E H -0.7702 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 R H -0.4460 Soluble
56 I H 0.0000 Buried
57 F H -0.6130 Soluble
58 P H 0.0000 Buried
59 G H -1.2414 Soluble
62 D H -2.2937 Soluble
63 G H -2.2335 Soluble
64 D H -2.3638 Soluble
65 T H -1.1626 Soluble
66 D H -0.4418 Soluble
67 Y H -0.2530 Soluble
68 N H 0.0000 Buried
69 G H -1.7212 Soluble
70 K H -2.4799 Soluble
71 F H 0.0000 Buried
72 K H -2.6255 Soluble
74 G H -1.7480 Soluble
75 R H -1.5269 Soluble
76 V H 0.0000 Buried
77 T H -0.6400 Soluble
78 I H 0.0000 Buried
79 T H -0.6644 Soluble
80 A H -1.3039 Soluble
81 D H -1.7382 Soluble
82 K H -2.2245 Soluble
83 S H -1.2783 Soluble
84 T H -1.1438 Soluble
85 S H -1.3041 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.6905 Soluble
89 M H 0.0000 Buried
90 E H -1.4656 Soluble
91 L H 0.0000 Buried
92 S H -1.0884 Soluble
93 S H -1.0994 Soluble
94 L H 0.0000 Buried
95 R H -2.6531 Soluble
96 S H -2.1667 Soluble
97 E H -2.4385 Soluble
98 D H 0.0000 Buried
99 T H -0.8472 Soluble
100 A H 0.0000 Buried
101 V H 0.4888 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.1207 Aggregation-prone
107 N H 0.4429 Aggregation-prone
108 V H 0.7732 Aggregation-prone
109 F H 0.6944 Aggregation-prone
110 D H -1.1049 Soluble
112 G H -0.4208 Soluble
113 Y H 0.2030 Aggregation-prone
114 W H 0.0000 Buried
115 L H 0.0000 Buried
116 V H 0.7470 Aggregation-prone
117 Y H 0.2765 Aggregation-prone
118 W H -0.1342 Soluble
119 G H 0.0000 Buried
120 Q H -1.1607 Soluble
121 G H -0.3661 Soluble
122 T H 0.0000 Buried
123 L H 0.7601 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.6626 Soluble
126 V H 0.0000 Buried
127 S H -1.0294 Soluble
128 S H -0.9417 Soluble
129 A H -0.5958 Soluble
130 S H -0.6307 Soluble
131 T H -0.7172 Soluble
132 K H -1.2032 Soluble
133 G H -1.4108 Soluble
134 P H 0.0000 Buried
135 S H -0.3771 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2928 Soluble
139 L H 0.0000 Buried
140 A H -1.5127 Soluble
141 P H 0.0000 Buried
142 S H -1.3637 Soluble
144 K H -1.8210 Soluble
145 S H 0.0000 Buried
146 T H -0.8758 Soluble
147 S H -0.7544 Soluble
148 G H -0.7967 Soluble
149 G H -0.8644 Soluble
150 T H -0.6066 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.3923 Soluble
159 D H -0.5359 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H -1.0287 Soluble
163 E H -1.4136 Soluble
164 P H -1.3973 Soluble
165 V H -1.0621 Soluble
166 T H -0.7825 Soluble
167 V H -0.1997 Soluble
168 S H -0.3647 Soluble
169 W H 0.0000 Buried
170 N H -0.7136 Soluble
171 S H -0.6085 Soluble
172 G H -0.4768 Soluble
173 A H -0.2414 Soluble
174 L H 0.0489 Aggregation-prone
175 T H -0.1613 Soluble
176 S H -0.1722 Soluble
177 G H -0.2172 Soluble
178 V H 0.1908 Aggregation-prone
179 H H -0.0912 Soluble
180 T H 0.0516 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.2378 Soluble
183 A H 0.0519 Aggregation-prone
184 V H 0.5262 Aggregation-prone
185 L H 1.0198 Aggregation-prone
186 Q H 0.2327 Aggregation-prone
187 S H -0.0616 Soluble
188 S H -0.1981 Soluble
189 G H 0.0132 Aggregation-prone
190 L H -0.1106 Soluble
191 Y H 0.0000 Buried
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1868 Soluble
199 V H 0.0000 Buried
200 P H -0.6876 Soluble
201 S H -0.6983 Soluble
202 S H -0.6333 Soluble
203 S H -0.7224 Soluble
204 L H -1.0013 Soluble
205 G H -1.1426 Soluble
206 T H -0.8721 Soluble
207 Q H -1.5844 Soluble
209 Y H -0.8528 Soluble
210 I H -0.9883 Soluble
211 C H 0.0000 Buried
212 N H -1.3864 Soluble
213 V H 0.0000 Buried
214 N H -2.1700 Soluble
215 H H 0.0000 Buried
216 K H -2.9449 Soluble
217 P H -1.8393 Soluble
218 S H -1.8578 Soluble
219 N H -2.6494 Soluble
220 T H -2.1569 Soluble
221 K H -2.6983 Soluble
222 V H -1.3909 Soluble
223 D H -2.2877 Soluble
224 K H -1.9439 Soluble
225 K H -2.4595 Soluble
227 E H -2.8750 Soluble
228 P H -1.9559 Soluble
229 K H -2.1544 Soluble
1 D L -1.7062 Soluble
2 I L 0.0000 Buried
3 V L 0.5964 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.3497 Soluble
6 Q L 0.0000 Buried
7 T L -0.0800 Soluble
8 P L 0.4179 Aggregation-prone
9 L L 1.1673 Aggregation-prone
10 S L 0.2902 Aggregation-prone
11 L L 0.0093 Aggregation-prone
12 P L -0.8119 Soluble
13 V L 0.0000 Buried
14 T L -1.6588 Soluble
15 P L -2.0813 Soluble
16 G L -2.0775 Soluble
17 E L -2.7938 Soluble
18 P L -2.3449 Soluble
19 A L -1.3406 Soluble
20 S L -0.7328 Soluble
21 I L 0.0000 Buried
22 S L -0.7583 Soluble
23 C L 0.0000 Buried
24 R L -2.3247 Soluble
25 S L -1.2666 Soluble
26 S L -1.1611 Soluble
27 K L -1.8568 Soluble
28 S L -0.9381 Soluble
29 L L 0.0000 Buried
30 L L 0.8424 Aggregation-prone
31 H L -0.2537 Soluble
32 S L -0.7936 Soluble
34 N L -1.3496 Soluble
35 G L -0.6115 Soluble
36 I L 0.3144 Aggregation-prone
37 T L 0.4977 Aggregation-prone
38 Y L 0.3620 Aggregation-prone
39 L L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 L L 0.0000 Buried
44 Q L -0.9011 Soluble
45 K L -1.4081 Soluble
46 P L -1.4581 Soluble
47 G L -1.4416 Soluble
48 Q L -1.9912 Soluble
49 S L -1.2962 Soluble
50 P L 0.0000 Buried
51 Q L -0.5390 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.0182 Soluble
56 Q L 0.1172 Aggregation-prone
57 M L -0.1041 Soluble
65 S L -0.6436 Soluble
66 N L -1.0272 Soluble
68 V L -0.1650 Soluble
69 S L -0.5359 Soluble
70 G L -0.6290 Soluble
71 V L -0.4661 Soluble
72 P L -1.0720 Soluble
74 D L -2.2245 Soluble
75 R L -2.0966 Soluble
76 F L 0.0000 Buried
77 S L -1.2329 Soluble
78 G L 0.0000 Buried
79 S L -0.7979 Soluble
80 G L -1.0118 Soluble
83 S L -0.6722 Soluble
84 G L -0.4977 Soluble
85 T L -1.4419 Soluble
86 D L -2.0512 Soluble
87 F L 0.0000 Buried
88 T L -0.9724 Soluble
89 L L 0.0000 Buried
90 K L -1.5674 Soluble
91 I L 0.0000 Buried
92 S L -2.3835 Soluble
93 R L -3.4200 Soluble
94 V L 0.0000 Buried
95 E L -2.5333 Soluble
96 A L -1.6085 Soluble
97 E L -2.2187 Soluble
98 D L 0.0000 Buried
99 V L -0.7494 Soluble
100 G L 0.0000 Buried
101 V L -0.1070 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 A L 0.0000 Buried
106 Q L 0.0000 Buried
107 N L -0.4069 Soluble
108 L L -0.3614 Soluble
109 E L -1.5674 Soluble
114 L L -0.4375 Soluble
115 P L -0.7507 Soluble
116 Y L 0.0000 Buried
117 T L -0.2052 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -0.5509 Soluble
121 G L -0.3166 Soluble
122 T L 0.0000 Buried
123 K L -0.4547 Soluble
124 V L 0.0000 Buried
125 E L -1.0642 Soluble
126 I L -1.1922 Soluble
127 K L -1.5595 Soluble
128 R L -1.2202 Soluble
129 T L -0.1983 Soluble
130 V L 0.3555 Aggregation-prone
131 A L 0.0101 Aggregation-prone
132 A L -0.1487 Soluble
133 P L 0.0000 Buried
134 S L -0.2550 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.9448 Soluble
140 P L -0.7299 Soluble
141 S L -1.5936 Soluble
142 D L -2.5965 Soluble
143 E L -2.5449 Soluble
144 Q L 0.0000 Buried
145 L L -2.0577 Soluble
146 K L -2.7457 Soluble
147 S L -1.7942 Soluble
148 G L -1.5815 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9216 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.2541 Soluble
162 R L -1.8389 Soluble
163 E L -2.5702 Soluble
164 A L -1.8295 Soluble
165 K L -1.9985 Soluble
166 V L -1.0842 Soluble
167 Q L -0.8065 Soluble
168 W L 0.0000 Buried
169 K L -0.4160 Soluble
170 V L 0.0000 Buried
171 D L -1.6142 Soluble
172 N L -1.3642 Soluble
173 A L -0.0918 Soluble
174 L L 0.7564 Aggregation-prone
175 Q L -0.0607 Soluble
176 S L -0.3829 Soluble
177 G L -0.8549 Soluble
178 N L -0.6995 Soluble
179 S L -1.0537 Soluble
180 Q L -1.2717 Soluble
181 E L -1.5781 Soluble
182 S L -0.7083 Soluble
183 V L -0.2507 Soluble
184 T L -0.9180 Soluble
185 E L -2.0782 Soluble
186 Q L -1.8289 Soluble
187 D L -2.0883 Soluble
188 S L -1.9939 Soluble
189 K L -2.3263 Soluble
190 D L -1.8934 Soluble
191 S L -1.8757 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L -0.3091 Soluble
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6196 Soluble
199 L L 0.0000 Buried
200 T L -0.3695 Soluble
201 L L -0.5444 Soluble
202 S L -1.1624 Soluble
203 K L -1.9650 Soluble
204 A L -1.7650 Soluble
205 D L -2.3490 Soluble
206 Y L 0.0000 Buried
207 E L -3.2819 Soluble
208 K L -3.4867 Soluble
209 H L -3.0110 Soluble
210 K L -3.2651 Soluble
211 V L -1.4735 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7902 Soluble
216 V L 0.0000 Buried
217 T L -1.1222 Soluble
218 H L 0.0000 Buried
219 Q L -1.5755 Soluble
220 G L -0.4438 Soluble
221 L L -0.2102 Soluble
222 S L -0.4369 Soluble
223 S L -0.4053 Soluble
224 P L -0.5115 Soluble
225 V L -0.0256 Soluble
226 T L -0.4534 Soluble
227 K L -0.9646 Soluble
228 S L -0.8952 Soluble
229 F L 0.0000 Buried
230 N L -2.0030 Soluble
231 R L -2.4897 Soluble
232 G L -2.1728 Soluble
233 E L -2.2671 Soluble
234 C L -1.0013 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.672 2.9451 CSV PDB
4.5 -0.7202 2.7549 CSV PDB
5.0 -0.7767 2.5501 CSV PDB
5.5 -0.8292 2.3403 CSV PDB
6.0 -0.8645 2.1289 CSV PDB
6.5 -0.8734 1.9176 CSV PDB
7.0 -0.8559 1.7144 CSV PDB
7.5 -0.8207 1.659 CSV PDB
8.0 -0.7755 1.607 CSV PDB
8.5 -0.723 1.6322 CSV PDB
9.0 -0.6638 1.6925 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH