Matuzumab / 3c09

A4DAB167_3c09_H_L | Source PDB: 3c09 | Chains: H, L | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.7353
Max Score
1.1053
Min Score
-4.1156
Total Score
-313.97
Score CDR
-0.4453
Score non-CDR
-0.7712

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGASVKVSCKASGYTFTSHWMHWVRQAPGQGLEWIGEFNPSNGRTNYNEKFKSKATMTVDTSTNTAYMELSSLRSEDTAVYYCASRDYDYAGRYFDYWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVGDRVTITCSASSSVTYMYWYQQKPGKAPKLLIYDTSNLASGVPSRFSGSGSGTDYTFTISSLQPEDIATYYCQQWSSHIFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.1156
Maximum Score 1.1053
Average Score -0.7353
Total Score -313.9692

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.3811 Soluble
2 V H -0.7573 Soluble
3 Q H -1.2381 Soluble
4 L H 0.0000 Buried
5 V H 0.2143 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.6333 Soluble
8 G H -1.0289 Soluble
9 A H -1.1807 Soluble
11 E H -1.7534 Soluble
12 V H -1.1014 Soluble
13 K H -1.7068 Soluble
14 K H -2.3742 Soluble
15 P H -2.1582 Soluble
16 G H -1.5045 Soluble
17 A H -1.0644 Soluble
18 S H -1.2657 Soluble
19 V H -1.2628 Soluble
20 K H -2.0753 Soluble
21 V H 0.0000 Buried
22 S H -0.6102 Soluble
23 C H 0.0000 Buried
24 K H -0.9825 Soluble
25 A H 0.0000 Buried
26 S H -0.8422 Soluble
27 G H -0.9494 Soluble
28 Y H -0.3919 Soluble
29 T H -0.1451 Soluble
30 F H 0.0000 Buried
35 T H -0.5596 Soluble
36 S H -0.4554 Soluble
37 H H -0.1609 Soluble
38 W H -0.2406 Soluble
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.4415 Soluble
44 Q H -0.6454 Soluble
45 A H 0.0000 Buried
46 P H -0.8804 Soluble
47 G H -1.2206 Soluble
48 Q H -1.8616 Soluble
49 G H -1.3750 Soluble
50 L H 0.0000 Buried
51 E H -1.0177 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 E H 0.0000 Buried
56 F H -0.5929 Soluble
57 N H -1.1797 Soluble
58 P H 0.0000 Buried
59 S H -1.2477 Soluble
62 N H -2.0891 Soluble
63 G H -1.8356 Soluble
64 R H -2.4473 Soluble
65 T H -1.2017 Soluble
66 N H -1.1840 Soluble
67 Y H -1.3375 Soluble
68 N H -2.2238 Soluble
69 E H -3.3931 Soluble
70 K H -3.2027 Soluble
71 F H 0.0000 Buried
72 K H -2.8778 Soluble
74 S H -1.7672 Soluble
75 K H -1.5702 Soluble
76 A H 0.0000 Buried
77 T H -1.0127 Soluble
78 M H 0.0000 Buried
79 T H -0.5745 Soluble
80 V H -0.2175 Soluble
81 D H -0.8673 Soluble
82 T H -0.6461 Soluble
83 S H -0.5680 Soluble
84 T H -0.7242 Soluble
85 N H -0.9778 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.6654 Soluble
89 M H 0.0000 Buried
90 E H -1.7860 Soluble
91 L H 0.0000 Buried
92 S H -1.0711 Soluble
93 S H -0.8908 Soluble
94 L H 0.0000 Buried
95 R H -2.8584 Soluble
96 S H -2.2634 Soluble
97 E H -2.4946 Soluble
98 D H 0.0000 Buried
99 T H -0.9038 Soluble
100 A H 0.0000 Buried
101 V H 0.1281 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H 0.0000 Buried
107 R H 0.0000 Buried
108 D H 0.0020 Aggregation-prone
109 Y H 0.1336 Aggregation-prone
110 D H -0.2234 Soluble
111 Y H 0.8173 Aggregation-prone
112A A H -0.0058 Soluble
112 G H -0.5882 Soluble
113 R H -1.3244 Soluble
114 Y H 0.0000 Buried
115 F H -0.1446 Soluble
116 D H -0.0300 Soluble
117 Y H 0.1656 Aggregation-prone
118 W H -0.3275 Soluble
119 G H 0.0000 Buried
120 Q H -1.4232 Soluble
121 G H -0.5852 Soluble
122 T H 0.0000 Buried
123 L H -0.4056 Soluble
124 V H 0.0000 Buried
125 T H -1.1213 Soluble
126 V H 0.0000 Buried
127 S H -1.2898 Soluble
128 S H -0.9278 Soluble
129 A H -0.6966 Soluble
130 S H -0.8049 Soluble
131 T H -1.0610 Soluble
132 K H -1.6957 Soluble
133 G H -1.6133 Soluble
134 P H -0.7966 Soluble
135 S H -0.5665 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0651 Soluble
139 L H 0.0000 Buried
140 A H -1.1334 Soluble
141 P H -0.7784 Soluble
142 S H -0.4557 Soluble
143 G H -0.6095 Soluble
144 T H -0.4934 Soluble
145 A H -0.2924 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H -0.3545 Soluble
153 D H -0.6048 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.8876 Soluble
158 P H -0.9566 Soluble
159 V H -0.6101 Soluble
160 T H -0.5545 Soluble
161 V H -0.1203 Soluble
162 S H -0.3886 Soluble
163 W H 0.0000 Buried
164 N H -0.8622 Soluble
165 S H -0.6988 Soluble
166 G H -0.5994 Soluble
167 A H -0.2944 Soluble
168 L H -0.0401 Soluble
169 T H -0.2181 Soluble
170 S H -0.2168 Soluble
171 G H -0.2985 Soluble
172 V H 0.1167 Aggregation-prone
173 H H -0.2797 Soluble
174 T H -0.0222 Soluble
175 F H 0.0000 Buried
176 P H -0.1782 Soluble
177 A H 0.2175 Aggregation-prone
178 V H 0.4400 Aggregation-prone
179 L H 1.1053 Aggregation-prone
180 Q H 0.2001 Aggregation-prone
181 S H -0.2443 Soluble
182 S H -0.2770 Soluble
183 G H -0.0754 Soluble
184 L H -0.0402 Soluble
185 Y H 0.4519 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1516 Soluble
193 V H 0.0000 Buried
194 P H -0.4611 Soluble
195 S H -0.5941 Soluble
196 S H -0.6132 Soluble
197 S H -0.6257 Soluble
198 L H -0.7193 Soluble
199 G H -0.9159 Soluble
200 T H -0.7468 Soluble
201 Q H -1.2113 Soluble
202 T H -1.0309 Soluble
203 Y H 0.0000 Buried
204 I H -1.1171 Soluble
205 C H 0.0000 Buried
206 N H -1.3849 Soluble
207 V H 0.0000 Buried
208 N H -1.8456 Soluble
209 H H 0.0000 Buried
210 K H -2.8552 Soluble
211 P H -2.0185 Soluble
212 S H -2.0760 Soluble
213 N H -2.5432 Soluble
214 T H -2.0524 Soluble
215 K H -2.6497 Soluble
216 V H -1.7152 Soluble
217 D H -2.4457 Soluble
218 K H -1.9916 Soluble
219 K H -2.1929 Soluble
220 V H 0.0000 Buried
221 E H -2.4175 Soluble
222 P H -1.7934 Soluble
223 K H -2.2193 Soluble
1 D L -2.3567 Soluble
2 I L 0.0000 Buried
3 Q L -1.7905 Soluble
4 M L 0.0000 Buried
5 T L -0.7107 Soluble
6 Q L 0.0000 Buried
7 S L -0.6703 Soluble
8 P L -0.7197 Soluble
9 S L -0.9900 Soluble
10 S L -0.9767 Soluble
11 L L -0.7049 Soluble
12 S L -1.0476 Soluble
13 A L -0.9534 Soluble
14 S L -0.8221 Soluble
15 V L 0.0261 Aggregation-prone
16 G L -0.5806 Soluble
17 D L -1.5409 Soluble
18 R L -2.2546 Soluble
19 V L 0.0000 Buried
20 T L -0.6362 Soluble
21 I L 0.0000 Buried
22 T L -0.5467 Soluble
23 C L 0.0000 Buried
24 S L -1.1168 Soluble
25 A L -1.1031 Soluble
26 S L -1.2471 Soluble
27 S L -0.9809 Soluble
28 S L -0.8214 Soluble
29 V L 0.0000 Buried
37 T L -0.0453 Soluble
38 Y L 0.2799 Aggregation-prone
39 M L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.4074 Soluble
45 K L -1.9169 Soluble
46 P L -1.5198 Soluble
47 G L -1.8009 Soluble
48 K L -2.5208 Soluble
49 A L -1.5640 Soluble
50 P L 0.0000 Buried
51 K L -1.6118 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.4597 Soluble
56 D L -0.7622 Soluble
57 T L -0.5833 Soluble
65 S L -0.7453 Soluble
66 N L -1.1250 Soluble
67 L L -0.1310 Soluble
68 A L -0.5237 Soluble
69 S L -0.4876 Soluble
70 G L -0.4372 Soluble
71 V L -0.1142 Soluble
72 P L -0.2892 Soluble
74 S L -0.3623 Soluble
75 R L -0.6931 Soluble
76 F L 0.0000 Buried
77 S L -0.3338 Soluble
78 G L 0.0000 Buried
79 S L -0.8838 Soluble
80 G L -1.1014 Soluble
83 S L -1.1617 Soluble
84 G L -1.2688 Soluble
85 T L -1.3912 Soluble
86 D L -2.1898 Soluble
87 Y L 0.0000 Buried
88 T L -0.6637 Soluble
89 F L 0.0000 Buried
90 T L -0.5842 Soluble
91 I L 0.0000 Buried
92 S L -1.2901 Soluble
93 S L -1.0254 Soluble
94 L L 0.0000 Buried
95 Q L -1.0598 Soluble
96 P L -1.5764 Soluble
97 E L -2.0596 Soluble
98 D L 0.0000 Buried
99 I L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -1.0727 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 W L 0.1734 Aggregation-prone
108 S L 0.0000 Buried
109 S L -0.7008 Soluble
114 H L -0.8808 Soluble
115 I L -0.6043 Soluble
116 F L 0.0000 Buried
117 T L -0.5624 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.7324 Soluble
121 G L -1.3615 Soluble
122 T L 0.0000 Buried
123 K L -1.9460 Soluble
124 V L 0.0000 Buried
125 E L -1.3951 Soluble
126 I L 0.0000 Buried
127 K L -1.3388 Soluble
128 R L -0.8236 Soluble
129 T L 0.1722 Aggregation-prone
130 V L 0.9968 Aggregation-prone
131 A L 0.2993 Aggregation-prone
132 A L 0.0434 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.1364 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6989 Soluble
140 P L 0.0000 Buried
141 S L -1.8034 Soluble
142 D L -2.9242 Soluble
143 E L -2.9233 Soluble
144 Q L 0.0000 Buried
145 L L -2.0337 Soluble
146 K L -2.7976 Soluble
147 S L -1.7424 Soluble
148 G L -1.1340 Soluble
149 T L -1.0397 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6943 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.4017 Soluble
162 R L -2.5148 Soluble
163 E L -3.0228 Soluble
164 A L -2.2431 Soluble
165 K L -2.2572 Soluble
166 V L -1.1673 Soluble
167 Q L -0.5657 Soluble
168 W L 0.0000 Buried
169 K L -0.5421 Soluble
170 V L 0.0000 Buried
171 D L -1.5069 Soluble
172 N L -1.4359 Soluble
173 A L -0.2110 Soluble
174 L L 0.8006 Aggregation-prone
175 Q L -0.1083 Soluble
176 S L -0.4943 Soluble
177 G L -0.9067 Soluble
178 N L -0.7694 Soluble
179 S L -1.0225 Soluble
180 Q L -1.2714 Soluble
181 E L -1.4338 Soluble
182 S L -0.6704 Soluble
183 V L -0.4426 Soluble
184 T L -0.9561 Soluble
185 E L -2.1342 Soluble
186 Q L 0.0000 Buried
187 D L -2.0668 Soluble
188 S L -2.2648 Soluble
189 K L -2.6317 Soluble
190 D L -1.9169 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6053 Soluble
199 L L 0.0000 Buried
200 T L -0.4902 Soluble
201 L L -0.8438 Soluble
202 S L -1.2735 Soluble
203 K L -2.1869 Soluble
204 A L -2.1575 Soluble
205 D L -3.1620 Soluble
206 Y L 0.0000 Buried
207 E L -4.1156 Soluble
208 K L -3.7924 Soluble
209 H L -3.3970 Soluble
210 K L -3.5806 Soluble
211 V L -1.7833 Soluble
212 Y L 0.0000 Buried
213 A L -0.7685 Soluble
214 C L 0.0000 Buried
215 E L -0.8949 Soluble
216 V L 0.0000 Buried
217 T L -1.1593 Soluble
218 H L 0.0000 Buried
219 Q L -1.4311 Soluble
220 G L -0.2771 Soluble
221 L L -0.1636 Soluble
222 S L -0.4290 Soluble
223 S L -0.3609 Soluble
224 P L -0.4539 Soluble
225 V L 0.2446 Aggregation-prone
226 T L -0.3456 Soluble
227 K L -0.5535 Soluble
228 S L -0.5491 Soluble
229 F L -1.0353 Soluble
230 N L -2.4124 Soluble
231 R L -3.1277 Soluble
232 G L -3.4742 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8607 1.4813 CSV PDB
4.5 -0.9051 1.4813 CSV PDB
5.0 -0.9557 1.4813 CSV PDB
5.5 -1.0012 1.4813 CSV PDB
6.0 -1.029 1.4813 CSV PDB
6.5 -1.0294 1.4813 CSV PDB
7.0 -1.0042 1.4813 CSV PDB
7.5 -0.9638 1.4813 CSV PDB
8.0 -0.9156 1.4813 CSV PDB
8.5 -0.8618 1.4813 CSV PDB
9.0 -0.8023 1.4813 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH