Pembrolizumab / 5dk3

A4DAB205_5dk3_G_F | Source PDB: 5dk3 | Chains: G, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7295
Max Score
1.5429
Min Score
-3.3437
Total Score
-478.53
Score CDR
-0.3694
Score non-CDR
-0.7598

Calculation Parameters

Chain Sequence(s) QVQLVQSGVEVKKPGASVKVSCKASGYTFTNYYMYWVRQAPGQGLEWMGGINPSNGGTNFNEKFKNRVTLTTDSSTTTAYMELKSLQFDDTAVYYCARRDYRFDMGFDYWGQGTTVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPPCGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSEIVLTQSPATLSLSPGERATLSCRASKGVSTSGYSYLHWYQQKPGQAPRLLIYLASYLESGVPARFSGSGSGTDFTLTISSLEPEDFAVYYCQHSRDLPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) G (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3437
Maximum Score 1.5429
Average Score -0.7295
Total Score -478.5278

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q G -1.3764 Soluble
2 V G -0.6781 Soluble
3 Q G -0.8097 Soluble
4 L G 0.0000 Buried
5 V G 0.3489 Aggregation-prone
6 Q G 0.0000 Buried
7 S G -0.2089 Soluble
8 G G 0.2657 Aggregation-prone
9 V G 1.4627 Aggregation-prone
11 E G 0.4319 Aggregation-prone
12 V G 0.2756 Aggregation-prone
13 K G -1.0738 Soluble
14 K G -1.9448 Soluble
15 P G -1.5153 Soluble
16 G G -1.3936 Soluble
17 A G -1.3698 Soluble
18 S G -1.7587 Soluble
19 V G 0.0000 Buried
20 K G -2.1683 Soluble
21 V G 0.0000 Buried
22 S G -0.5129 Soluble
23 C G 0.0000 Buried
24 K G -0.8157 Soluble
25 A G 0.0000 Buried
26 S G -0.7155 Soluble
27 G G -0.9188 Soluble
28 Y G -0.4967 Soluble
29 T G -0.4214 Soluble
30 F G 0.0000 Buried
35 T G -0.7202 Soluble
36 N G -1.3040 Soluble
37 Y G -0.3024 Soluble
38 Y G -0.1900 Soluble
39 M G 0.0000 Buried
40 Y G 0.0000 Buried
41 W G 0.0000 Buried
42 V G 0.0000 Buried
43 R G 0.0000 Buried
44 Q G -0.7625 Soluble
45 A G -1.0986 Soluble
46 P G -1.0574 Soluble
47 G G -1.2539 Soluble
48 Q G -1.8157 Soluble
49 G G -1.2217 Soluble
50 L G 0.0000 Buried
51 E G -1.0327 Soluble
52 W G 0.0000 Buried
53 M G 0.0000 Buried
54 G G 0.0000 Buried
55 G G 0.0000 Buried
56 I G 0.0000 Buried
57 N G 0.0000 Buried
58 P G -0.9285 Soluble
59 S G -1.1690 Soluble
62 N G -1.6170 Soluble
63 G G -1.2052 Soluble
64 G G -0.9509 Soluble
65 T G -0.5841 Soluble
66 N G -0.7695 Soluble
67 F G -1.2269 Soluble
68 N G -2.0386 Soluble
69 E G -3.2851 Soluble
70 K G -3.2012 Soluble
71 F G 0.0000 Buried
72 K G -3.2532 Soluble
74 N G -2.7643 Soluble
75 R G -2.1289 Soluble
76 V G 0.0000 Buried
77 T G -1.1066 Soluble
78 L G 0.0000 Buried
79 T G -0.3277 Soluble
80 T G -0.8140 Soluble
81 D G -1.2267 Soluble
82 S G -0.8252 Soluble
83 S G -0.6109 Soluble
84 T G -0.6970 Soluble
85 T G -0.8114 Soluble
86 T G 0.0000 Buried
87 A G 0.0000 Buried
88 Y G -0.5114 Soluble
89 M G 0.0000 Buried
90 E G -1.7250 Soluble
91 L G 0.0000 Buried
92 K G -2.2907 Soluble
93 S G -1.6804 Soluble
94 L G 0.0000 Buried
95 Q G -1.5025 Soluble
96 F G 0.0935 Aggregation-prone
97 D G -1.2662 Soluble
98 D G 0.0000 Buried
99 T G -0.2912 Soluble
100 A G 0.0000 Buried
101 V G 0.1603 Aggregation-prone
102 Y G 0.0000 Buried
103 Y G 0.0000 Buried
104 C G 0.0000 Buried
105 A G 0.0000 Buried
106 R G 0.0000 Buried
107 R G -0.0984 Soluble
108 D G -0.2006 Soluble
109 Y G 0.1656 Aggregation-prone
110 R G -0.7876 Soluble
111 F G 1.0485 Aggregation-prone
112 D G 0.4908 Aggregation-prone
113 M G 0.6487 Aggregation-prone
114 G G 0.0000 Buried
115 F G 0.0000 Buried
116 D G -0.2705 Soluble
117 Y G 0.0920 Aggregation-prone
118 W G -0.1680 Soluble
119 G G 0.0000 Buried
120 Q G -1.2568 Soluble
121 G G -0.4940 Soluble
122 T G 0.0000 Buried
123 T G 0.3657 Aggregation-prone
124 V G 0.0000 Buried
125 T G -0.0267 Soluble
126 V G 0.0000 Buried
127 S G 0.0000 Buried
128 S G -0.6122 Soluble
129 A G -0.7205 Soluble
130 S G -0.8036 Soluble
131 T G -0.8343 Soluble
132 K G -1.6475 Soluble
133 G G -1.5775 Soluble
134 P G -0.7372 Soluble
135 S G -0.5299 Soluble
136 V G 0.0000 Buried
137 F G 0.0000 Buried
138 P G -0.8285 Soluble
139 L G 0.0000 Buried
140 A G -0.0727 Soluble
141 P G -0.0196 Soluble
142 C G -0.2146 Soluble
143 S G -0.4599 Soluble
144 R G -0.8018 Soluble
145 S G -0.7946 Soluble
146 T G -1.2685 Soluble
147 S G -1.9593 Soluble
148 E G -2.5898 Soluble
149 S G -1.6649 Soluble
150 T G -0.8913 Soluble
151 A G 0.0000 Buried
152 A G 0.0000 Buried
153 L G 0.0000 Buried
154 G G 0.0000 Buried
155 C G 0.0000 Buried
156 L G 0.0000 Buried
157 V G 0.0000 Buried
158 K G 0.0000 Buried
159 D G -0.6336 Soluble
160 Y G 0.0000 Buried
161 F G 0.0000 Buried
162 P G 0.0000 Buried
163 E G -0.5824 Soluble
164 P G -0.9606 Soluble
165 V G -0.7827 Soluble
166 T G -0.5900 Soluble
167 V G -0.1322 Soluble
168 S G -0.4730 Soluble
169 W G 0.0000 Buried
170 N G -1.0110 Soluble
171 S G -0.8192 Soluble
172 G G -0.5990 Soluble
173 A G -0.2738 Soluble
174 L G -0.0657 Soluble
175 T G -0.1608 Soluble
176 S G -0.1419 Soluble
177 G G -0.1234 Soluble
178 V G 0.2341 Aggregation-prone
179 H G -0.2881 Soluble
180 T G 0.0148 Aggregation-prone
181 F G 0.0000 Buried
182 P G -0.3988 Soluble
183 A G 0.0914 Aggregation-prone
184 V G 0.4384 Aggregation-prone
185 L G 1.0718 Aggregation-prone
186 Q G 0.2437 Aggregation-prone
187 S G -0.0666 Soluble
188 S G -0.2319 Soluble
189 G G 0.0319 Aggregation-prone
190 L G -0.1089 Soluble
191 Y G 0.2160 Aggregation-prone
192 S G 0.0000 Buried
193 L G 0.0000 Buried
194 S G 0.0000 Buried
195 S G 0.0000 Buried
196 V G 0.0000 Buried
197 V G 0.0000 Buried
198 T G -0.1056 Soluble
199 V G 0.0000 Buried
200 P G -0.5874 Soluble
201 S G -0.7747 Soluble
202 S G -0.7221 Soluble
203 S G 0.0000 Buried
204 L G -0.7544 Soluble
205 G G -0.9665 Soluble
206 T G -1.1366 Soluble
207 K G -1.8497 Soluble
208 T G -1.4875 Soluble
209 Y G 0.0000 Buried
210 T G -1.4120 Soluble
211 C G 0.0000 Buried
212 N G -1.6064 Soluble
213 V G 0.0000 Buried
214 D G -1.8776 Soluble
215 H G 0.0000 Buried
216 K G -2.7969 Soluble
217 P G -1.7834 Soluble
218 S G -1.9363 Soluble
219 N G -2.6329 Soluble
220 T G -2.0742 Soluble
221 K G -2.6506 Soluble
222 V G -1.5975 Soluble
223 D G -2.6307 Soluble
224 K G -2.1269 Soluble
225 R G -2.2866 Soluble
226 V G -1.1500 Soluble
227 E G -1.3362 Soluble
228 S G -1.3800 Soluble
229 K G -1.5765 Soluble
230 Y G 0.1115 Aggregation-prone
231 G G -0.0338 Soluble
232 P G -0.3064 Soluble
233 P G -0.1661 Soluble
234 C G 0.1298 Aggregation-prone
235 P G 0.0423 Aggregation-prone
236 P G 0.1576 Aggregation-prone
237 C G 0.5926 Aggregation-prone
238 G G -0.8803 Soluble
239 G G -0.8669 Soluble
240 P G -0.6424 Soluble
241 S G -0.2410 Soluble
242 V G 0.0000 Buried
243 F G 1.2069 Aggregation-prone
244 L G 0.9235 Aggregation-prone
245 F G 1.0779 Aggregation-prone
246 P G -0.1445 Soluble
247 P G 0.0000 Buried
248 K G -2.2018 Soluble
249 P G -1.3742 Soluble
250 K G -1.2777 Soluble
251 D G 0.0000 Buried
252 T G 0.0000 Buried
253 L G 0.0000 Buried
254 M G 0.1812 Aggregation-prone
255 I G 1.3649 Aggregation-prone
256 S G -0.1395 Soluble
257 R G -1.5767 Soluble
258 T G -0.8805 Soluble
259 P G 0.0000 Buried
260 E G -0.9792 Soluble
261 V G 0.0000 Buried
262 T G 0.2036 Aggregation-prone
263 C G 0.0000 Buried
264 V G 0.0000 Buried
265 V G 0.0000 Buried
266 V G -0.1995 Soluble
267 D G -1.4491 Soluble
268 V G 0.0000 Buried
269 S G -1.7514 Soluble
270 Q G -2.0103 Soluble
271 E G -2.7608 Soluble
272 D G -1.8281 Soluble
273 P G -1.6958 Soluble
274 E G -1.0765 Soluble
275 V G -0.1320 Soluble
276 Q G -0.7944 Soluble
277 F G -0.5396 Soluble
278 N G -1.0687 Soluble
279 W G 0.0000 Buried
280 Y G -0.5730 Soluble
281 V G -0.5615 Soluble
282 D G -1.6131 Soluble
283 G G -0.6435 Soluble
284 V G 0.7427 Aggregation-prone
285 E G -0.5938 Soluble
286 V G -0.6819 Soluble
287 H G -1.8827 Soluble
288 N G -2.1812 Soluble
289 A G -1.8809 Soluble
290 K G -2.3533 Soluble
291 T G -1.5179 Soluble
292 K G -1.7532 Soluble
293 P G -1.4508 Soluble
294 R G -2.5813 Soluble
295 E G -2.9800 Soluble
296 E G -2.9005 Soluble
297 Q G -1.6927 Soluble
298 F G 0.3298 Aggregation-prone
299 N G -1.2113 Soluble
300 S G -1.1167 Soluble
301 T G -1.7248 Soluble
302 Y G -2.1782 Soluble
303 R G -1.9394 Soluble
304 V G 0.0000 Buried
305 V G -0.5370 Soluble
306 S G 0.0000 Buried
307 V G 0.0000 Buried
308 L G 0.0000 Buried
309 T G -0.6809 Soluble
310 V G 0.0000 Buried
311 L G 0.8651 Aggregation-prone
312 H G -0.1874 Soluble
313 Q G -1.2301 Soluble
314 D G -1.4743 Soluble
315 W G 0.0000 Buried
316 L G -1.0180 Soluble
317 N G -2.1992 Soluble
318 G G -2.0184 Soluble
319 K G -2.1155 Soluble
320 E G -1.8237 Soluble
321 Y G 0.0000 Buried
322 K G -1.3767 Soluble
323 C G 0.0000 Buried
324 K G -1.5317 Soluble
325 V G 0.0000 Buried
326 S G -0.9552 Soluble
327 N G 0.0000 Buried
328 K G -2.3381 Soluble
329 G G -1.5988 Soluble
330 L G -0.7399 Soluble
331 P G -0.6365 Soluble
332 S G -0.5410 Soluble
333 S G -0.7949 Soluble
334 I G -0.7720 Soluble
335 E G -1.7639 Soluble
336 K G -1.1101 Soluble
337 T G -0.8341 Soluble
338 I G -0.1785 Soluble
339 S G -1.0191 Soluble
340 K G -1.2825 Soluble
341 A G -1.1832 Soluble
342 K G -2.3360 Soluble
343 G G -1.9620 Soluble
344 Q G -1.9300 Soluble
345 P G -1.7436 Soluble
346 R G -2.1150 Soluble
347 E G -2.5886 Soluble
348 P G 0.0000 Buried
349 Q G -1.4076 Soluble
350 V G 0.0000 Buried
351 Y G 0.2671 Aggregation-prone
352 T G 0.1853 Aggregation-prone
353 L G 0.4980 Aggregation-prone
354 P G -0.0680 Soluble
355 P G -0.7448 Soluble
356 S G -1.3633 Soluble
357 Q G -2.2231 Soluble
358 E G -2.7899 Soluble
359 E G -2.3553 Soluble
360 M G -2.0928 Soluble
361 T G -1.9921 Soluble
362 K G -3.1039 Soluble
363 N G -2.9342 Soluble
364 Q G -2.7689 Soluble
365 V G 0.0000 Buried
366 S G -0.7485 Soluble
367 L G 0.0000 Buried
368 T G -0.1140 Soluble
369 C G 0.0000 Buried
370 L G 0.3200 Aggregation-prone
371 V G 0.0000 Buried
372 K G -0.7563 Soluble
373 G G -1.3719 Soluble
374 F G 0.0000 Buried
375 Y G -1.0566 Soluble
376 P G 0.0000 Buried
377 S G -0.0755 Soluble
378 D G -1.2056 Soluble
379 I G -0.6222 Soluble
380 A G 0.0000 Buried
381 V G -0.2032 Soluble
382 E G -1.2154 Soluble
383 W G 0.0000 Buried
384 E G -1.9424 Soluble
385 S G 0.0000 Buried
386 N G -1.9027 Soluble
387 G G -1.7949 Soluble
388 Q G -2.1499 Soluble
389 P G -1.8706 Soluble
390 E G -2.0535 Soluble
391 N G -2.3962 Soluble
392 N G -2.4155 Soluble
393 Y G -2.1879 Soluble
394 K G -2.4916 Soluble
395 T G -1.1606 Soluble
396 T G -0.4218 Soluble
397 P G 0.0987 Aggregation-prone
398 P G 0.6022 Aggregation-prone
399 V G 1.5429 Aggregation-prone
400 L G 1.1730 Aggregation-prone
401 D G -0.8316 Soluble
402 S G -1.2347 Soluble
403 D G -2.1279 Soluble
404 G G -1.1746 Soluble
405 S G 0.0000 Buried
406 F G 0.3422 Aggregation-prone
407 F G 0.6656 Aggregation-prone
408 L G 0.0000 Buried
409 Y G 0.1698 Aggregation-prone
410 S G 0.0000 Buried
411 R G -1.9128 Soluble
412 L G 0.0000 Buried
413 T G -1.6122 Soluble
414 V G 0.0000 Buried
415 D G -2.8288 Soluble
416 K G -2.9484 Soluble
417 S G -2.5884 Soluble
418 R G -2.8914 Soluble
419 W G 0.0000 Buried
420 Q G -2.3741 Soluble
421 Q G -2.3772 Soluble
422 G G -1.4637 Soluble
423 N G -1.3364 Soluble
424 V G -0.4924 Soluble
425 F G 0.0000 Buried
426 S G -1.2157 Soluble
427 C G 0.0000 Buried
428 S G 0.0000 Buried
429 V G 0.0000 Buried
430 M G 0.0000 Buried
431 H G 0.0000 Buried
432 E G -1.0959 Soluble
433 A G -1.4188 Soluble
434 L G -1.3955 Soluble
435 H G -1.7357 Soluble
436 N G -1.6714 Soluble
437 H G -0.9768 Soluble
438 Y G -0.3674 Soluble
439 T G -0.7697 Soluble
440 Q G -1.1179 Soluble
441 K G -1.1775 Soluble
442 S G -0.7452 Soluble
443 L G 0.0000 Buried
444 S G -0.1234 Soluble
445 L G -0.2868 Soluble
446 S G -0.1647 Soluble
1 E F -1.7485 Soluble
2 I F 0.0000 Buried
3 V F 0.6797 Aggregation-prone
4 L F 0.0000 Buried
5 T F -0.6634 Soluble
6 Q F -0.8249 Soluble
7 S F -0.6556 Soluble
8 P F -0.2801 Soluble
9 A F -0.2428 Soluble
10 T F -0.1684 Soluble
11 L F -0.1053 Soluble
12 S F -0.6477 Soluble
13 L F -1.1167 Soluble
14 S F -1.4941 Soluble
15 P F -1.5860 Soluble
16 G F -1.6283 Soluble
17 E F -2.2943 Soluble
18 R F -2.5488 Soluble
19 A F 0.0000 Buried
20 T F -0.5680 Soluble
21 L F 0.0000 Buried
22 S F -0.9108 Soluble
23 C F 0.0000 Buried
24 R F -2.4529 Soluble
25 A F 0.0000 Buried
26 S F -1.3343 Soluble
27 K F -2.1938 Soluble
28 G F -1.6533 Soluble
29 V F 0.0000 Buried
30 S F -0.4892 Soluble
31 T F -0.2562 Soluble
34 S F -0.2448 Soluble
35 G F -0.2285 Soluble
36 Y F 0.3403 Aggregation-prone
37 S F -0.0189 Soluble
38 Y F -0.0627 Soluble
39 L F 0.0000 Buried
40 H F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F 0.0000 Buried
45 K F -1.4200 Soluble
46 P F -1.3936 Soluble
47 G F -1.4543 Soluble
48 Q F -2.0764 Soluble
49 A F -1.2652 Soluble
50 P F 0.0000 Buried
51 R F -1.0126 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 Y F 0.6963 Aggregation-prone
56 L F 0.0000 Buried
57 A F 0.0000 Buried
65 S F 0.4887 Aggregation-prone
66 Y F 1.3761 Aggregation-prone
67 L F 1.0709 Aggregation-prone
68 E F 0.1266 Aggregation-prone
69 S F -0.2856 Soluble
70 G F -0.5244 Soluble
71 V F 0.0000 Buried
72 P F -0.2105 Soluble
74 A F -0.1120 Soluble
75 R F -0.6653 Soluble
76 F F 0.0000 Buried
77 S F 0.0094 Aggregation-prone
78 G F 0.0153 Aggregation-prone
79 S F -0.5136 Soluble
80 G F -0.8778 Soluble
83 S F -0.7433 Soluble
84 G F -1.1906 Soluble
85 T F -1.9420 Soluble
86 D F -2.2087 Soluble
87 F F 0.0000 Buried
88 T F -0.8072 Soluble
89 L F 0.0000 Buried
90 T F -0.6140 Soluble
91 I F 0.0000 Buried
92 S F -1.2593 Soluble
93 S F -1.5453 Soluble
94 L F 0.0000 Buried
95 E F -1.7779 Soluble
96 P F -1.2809 Soluble
97 E F -1.6910 Soluble
98 D F 0.0000 Buried
99 F F -0.4318 Soluble
100 A F 0.0000 Buried
101 V F 0.0034 Aggregation-prone
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 H F 0.0000 Buried
107 S F -0.5328 Soluble
108 R F -1.3017 Soluble
109 D F -1.5562 Soluble
114 L F -0.6486 Soluble
115 P F -0.7195 Soluble
116 L F 0.0000 Buried
117 T F -0.0432 Soluble
118 F F 0.1343 Aggregation-prone
119 G F 0.0000 Buried
120 G F -0.5716 Soluble
121 G F -0.5350 Soluble
122 T F 0.0000 Buried
123 K F -0.4074 Soluble
124 V F 0.0000 Buried
125 E F 0.0000 Buried
126 I F -0.9431 Soluble
127 K F -1.8547 Soluble
128 R F -1.3265 Soluble
129 T F -0.3758 Soluble
130 V F 0.1239 Aggregation-prone
131 A F -0.0433 Soluble
132 A F -0.1671 Soluble
133 P F 0.0000 Buried
134 S F -0.2471 Soluble
135 V F 0.0000 Buried
136 F F 0.0000 Buried
137 I F -0.0264 Soluble
138 F F 0.0000 Buried
139 P F -0.2402 Soluble
140 P F 0.0000 Buried
141 S F -1.5128 Soluble
142 D F -2.8192 Soluble
143 E F -2.7574 Soluble
144 Q F 0.0000 Buried
145 L F -2.0680 Soluble
146 K F -2.6866 Soluble
147 S F -1.6577 Soluble
148 G F -1.1462 Soluble
149 T F -0.7626 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -0.7011 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F -1.8301 Soluble
162 R F -2.8543 Soluble
163 E F -3.1477 Soluble
164 A F -2.3026 Soluble
165 K F -2.4021 Soluble
166 V F -1.3435 Soluble
167 Q F -0.8487 Soluble
168 W F 0.0000 Buried
169 K F -0.4990 Soluble
170 V F 0.0000 Buried
171 D F -1.4203 Soluble
172 N F -1.2597 Soluble
173 A F -0.1710 Soluble
174 L F 0.7512 Aggregation-prone
175 Q F -0.1138 Soluble
176 S F -0.4440 Soluble
177 G F -0.9226 Soluble
178 N F -0.8573 Soluble
179 S F -1.2199 Soluble
180 Q F -1.4757 Soluble
181 E F -2.0240 Soluble
182 S F -0.9108 Soluble
183 V F -0.9301 Soluble
184 T F -1.1835 Soluble
185 E F -2.2869 Soluble
186 Q F -1.8395 Soluble
187 D F -1.9034 Soluble
188 S F -1.7117 Soluble
189 K F -2.2032 Soluble
190 D F -1.6243 Soluble
191 S F -1.7007 Soluble
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.7007 Soluble
199 L F 0.0000 Buried
200 T F -0.4194 Soluble
201 L F -0.6357 Soluble
202 S F -1.1480 Soluble
203 K F -1.8537 Soluble
204 A F -1.8078 Soluble
205 D F -2.5583 Soluble
206 Y F 0.0000 Buried
207 E F -3.2907 Soluble
208 K F -3.3437 Soluble
209 H F -2.5907 Soluble
210 K F -2.6198 Soluble
211 V F -1.0713 Soluble
212 Y F 0.0000 Buried
213 A F 0.0000 Buried
214 C F 0.0000 Buried
215 E F -1.0064 Soluble
216 V F 0.0000 Buried
217 T F -1.2614 Soluble
218 H F 0.0000 Buried
219 Q F -1.4048 Soluble
220 G F -0.5440 Soluble
221 L F -0.2692 Soluble
222 S F -0.4630 Soluble
223 S F -0.4284 Soluble
224 P F -0.5384 Soluble
225 V F -0.1415 Soluble
226 T F -0.5811 Soluble
227 K F -0.8108 Soluble
228 S F -0.4325 Soluble
229 F F 0.0000 Buried
230 N F -1.4851 Soluble
231 R F -1.8628 Soluble
232 G F -1.8618 Soluble
233 E F -2.0637 Soluble
234 C F -0.4687 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6492 4.6122 CSV PDB
4.5 -0.6978 4.6122 CSV PDB
5.0 -0.7563 4.6122 CSV PDB
5.5 -0.8129 4.6122 CSV PDB
6.0 -0.8536 4.6122 CSV PDB
6.5 -0.8677 4.6122 CSV PDB
7.0 -0.8545 4.6122 CSV PDB
7.5 -0.8227 4.6122 CSV PDB
8.0 -0.7806 4.6122 CSV PDB
8.5 -0.7315 4.6121 CSV PDB
9.0 -0.6763 4.6118 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH