Vilastobart / 8g8n

A4DAB294_8g8n_G_I | Source PDB: 8g8n | Chains: G, I | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7574
Max Score
2.4065
Min Score
-3.6394
Total Score
-321.91
Score CDR
-0.5819
Score non-CDR
-0.7767

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGSSVKVSCKASGYTFTNYFMNWVRQAPGQGLEWMGRVDPEQGRADYAEKFKKRVTITADKSTSTAYMELSSLRSEDTAVYYCARRAMDNYGFAYWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCIVLTQSPDFQSVTPKEKVTITCSANSYMYWYQQKPDQSPKLWVHGTSNLASGVPSRFSGSGSGTDFTLTINSLEAEDAATYYCHHWSNTQWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) G (Heavy), I (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6394
Maximum Score 2.4065
Average Score -0.7574
Total Score -321.9085

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q G -1.6533 Soluble
2 V G 0.0000 Buried
3 Q G -1.4522 Soluble
4 L G 0.0000 Buried
5 V G 0.3625 Aggregation-prone
6 Q G 0.0000 Buried
7 S G -0.6308 Soluble
8 G G -0.7039 Soluble
9 A G -0.5650 Soluble
11 E G -1.2205 Soluble
12 V G -0.6344 Soluble
13 K G -1.5193 Soluble
14 K G -2.2761 Soluble
15 P G -1.9394 Soluble
16 G G -1.4257 Soluble
17 S G -1.1487 Soluble
18 S G -1.2771 Soluble
19 V G 0.0000 Buried
20 K G -2.1032 Soluble
21 V G 0.0000 Buried
22 S G -0.5529 Soluble
23 C G 0.0000 Buried
24 K G -0.8276 Soluble
25 A G 0.0000 Buried
26 S G -0.8976 Soluble
27 G G -1.1761 Soluble
28 Y G -0.6989 Soluble
29 T G -0.6304 Soluble
30 F G 0.0000 Buried
35 T G -1.9347 Soluble
36 N G -1.5969 Soluble
37 Y G 0.0000 Buried
38 F G -0.4506 Soluble
39 M G 0.0000 Buried
40 N G 0.0000 Buried
41 W G 0.0000 Buried
42 V G 0.0000 Buried
43 R G 0.0000 Buried
44 Q G -0.6152 Soluble
45 A G -1.0026 Soluble
46 P G -1.0026 Soluble
47 G G -1.2438 Soluble
48 Q G -1.7232 Soluble
49 G G -1.0974 Soluble
50 L G 0.0000 Buried
51 E G -0.8817 Soluble
52 W G 0.0000 Buried
53 M G 0.0000 Buried
54 G G 0.0000 Buried
55 R G 0.0000 Buried
56 V G 0.0000 Buried
57 D G -1.9455 Soluble
58 P G 0.0000 Buried
59 E G -3.4894 Soluble
62 Q G -3.0651 Soluble
63 G G -2.7279 Soluble
64 R G -2.7871 Soluble
65 A G -1.2596 Soluble
66 D G -1.1994 Soluble
67 Y G -1.4883 Soluble
68 A G -2.2103 Soluble
69 E G -3.5187 Soluble
70 K G -3.4221 Soluble
71 F G 0.0000 Buried
72 K G -3.6394 Soluble
74 K G -3.2501 Soluble
75 R G -1.9178 Soluble
76 V G 0.0000 Buried
77 T G -1.0495 Soluble
78 I G 0.0000 Buried
79 T G -0.7932 Soluble
80 A G -1.5509 Soluble
81 D G -1.9106 Soluble
82 K G -2.6838 Soluble
83 S G -1.2861 Soluble
84 T G -1.1005 Soluble
85 S G -1.2605 Soluble
86 T G -0.9131 Soluble
87 A G 0.0000 Buried
88 Y G -0.5982 Soluble
89 M G 0.0000 Buried
90 E G -1.4380 Soluble
91 L G 0.0000 Buried
92 S G -1.1525 Soluble
93 S G -1.1592 Soluble
94 L G 0.0000 Buried
95 R G -2.3020 Soluble
96 S G -2.0088 Soluble
97 E G -2.2711 Soluble
98 D G 0.0000 Buried
99 T G -0.8160 Soluble
100 A G 0.0000 Buried
101 V G 0.2243 Aggregation-prone
102 Y G 0.0000 Buried
103 Y G 0.0000 Buried
104 C G 0.0000 Buried
105 A G 0.0000 Buried
106 R G 0.0000 Buried
107 R G 0.0273 Aggregation-prone
108 A G 0.0000 Buried
109 M G 0.2550 Aggregation-prone
110 D G -1.2104 Soluble
112 N G -0.4985 Soluble
113 Y G 0.8002 Aggregation-prone
114 G G 0.0000 Buried
115 F G 0.0000 Buried
116 A G -0.0917 Soluble
117 Y G -0.1892 Soluble
118 W G -0.3199 Soluble
119 G G 0.0000 Buried
120 Q G -1.1398 Soluble
121 G G -0.4981 Soluble
122 T G 0.0000 Buried
123 L G -0.1858 Soluble
124 V G 0.0000 Buried
125 T G 0.0000 Buried
126 V G 0.0000 Buried
127 S G -1.2704 Soluble
128 S G -0.9388 Soluble
129 A G -0.6106 Soluble
130 S G -0.6590 Soluble
131 T G -0.5567 Soluble
132 K G -1.2123 Soluble
133 G G -1.4624 Soluble
134 P G -0.7455 Soluble
135 S G -0.4147 Soluble
136 V G 0.0000 Buried
137 F G 0.0000 Buried
138 P G -1.1338 Soluble
139 L G 0.0000 Buried
140 A G -0.7923 Soluble
141 P G -0.4468 Soluble
142 S G -0.8620 Soluble
143 G G -0.6515 Soluble
144 T G -0.5072 Soluble
145 A G -0.2418 Soluble
146 A G 0.0000 Buried
147 L G 0.0000 Buried
148 G G 0.0000 Buried
149 C G 0.0000 Buried
150 L G 0.0000 Buried
151 V G 0.0000 Buried
152 K G -0.3443 Soluble
153 D G -0.4413 Soluble
154 Y G 0.0000 Buried
155 F G 0.0000 Buried
156 P G 0.0000 Buried
157 E G -0.2809 Soluble
158 P G -0.7266 Soluble
159 V G -0.8237 Soluble
160 T G -0.7568 Soluble
161 V G -0.4726 Soluble
162 S G -0.4942 Soluble
163 W G 0.0000 Buried
164 N G -0.7836 Soluble
165 S G -0.6711 Soluble
166 G G -0.5804 Soluble
167 A G -0.2624 Soluble
168 L G -0.0332 Soluble
169 T G -0.1892 Soluble
170 S G -0.3539 Soluble
171 G G -0.2243 Soluble
172 V G 0.1629 Aggregation-prone
173 H G 0.0000 Buried
174 T G -0.0812 Soluble
175 F G 0.0000 Buried
176 P G -0.1383 Soluble
177 A G 0.4331 Aggregation-prone
178 V G 0.6373 Aggregation-prone
179 L G 1.4258 Aggregation-prone
180 Q G 0.2567 Aggregation-prone
181 S G -0.0453 Soluble
182 S G -0.1940 Soluble
183 G G 0.0629 Aggregation-prone
184 L G 0.1094 Aggregation-prone
185 Y G 0.0000 Buried
186 S G 0.2428 Aggregation-prone
187 L G 0.0000 Buried
188 S G 0.0000 Buried
189 S G 0.0000 Buried
190 V G 0.0000 Buried
191 V G 0.0000 Buried
192 T G -0.1480 Soluble
193 V G 0.0000 Buried
194 P G -0.5949 Soluble
195 S G -0.5426 Soluble
196 S G -0.5784 Soluble
197 S G -0.5614 Soluble
198 L G -0.7625 Soluble
199 G G -1.2987 Soluble
200 T G -0.8690 Soluble
201 Q G -1.1663 Soluble
202 T G -1.1001 Soluble
203 Y G 0.0000 Buried
204 I G -1.1473 Soluble
205 C G 0.0000 Buried
206 N G -1.5980 Soluble
207 V G 0.0000 Buried
208 N G -2.5876 Soluble
209 H G 0.0000 Buried
210 K G -2.9073 Soluble
211 P G -1.7546 Soluble
212 S G -1.9350 Soluble
213 N G -2.6836 Soluble
214 T G -2.2655 Soluble
215 K G -2.9056 Soluble
216 V G -1.6977 Soluble
217 D G -2.4271 Soluble
218 K G -1.8563 Soluble
219 K G -2.3928 Soluble
220 V G 0.0000 Buried
221 E G -2.6478 Soluble
222 P G -1.4523 Soluble
223 K G -1.8256 Soluble
224 S G -0.5386 Soluble
225 C G 0.2200 Aggregation-prone
2 I I 2.3840 Aggregation-prone
3 V I 2.4065 Aggregation-prone
4 L I 0.0000 Buried
5 T I -0.1537 Soluble
6 Q I 0.0000 Buried
7 S I -0.9515 Soluble
8 P I -1.1475 Soluble
9 D I -1.9614 Soluble
10 F I -0.7986 Soluble
11 Q I -0.9558 Soluble
12 S I -0.7978 Soluble
13 V I 0.0000 Buried
14 T I -2.0999 Soluble
15 P I -2.7410 Soluble
16 K I -3.3785 Soluble
17 E I -3.6291 Soluble
18 K I -3.1395 Soluble
19 V I 0.0000 Buried
20 T I -0.6015 Soluble
21 I I 0.0000 Buried
22 T I -0.7790 Soluble
23 C I 0.0000 Buried
24 S I -0.9513 Soluble
25 A I -0.3243 Soluble
26 N I -0.7264 Soluble
27 S I 0.0611 Aggregation-prone
38 Y I 1.2025 Aggregation-prone
39 M I 0.0000 Buried
40 Y I 0.0000 Buried
41 W I 0.0000 Buried
42 Y I 0.0000 Buried
43 Q I -0.9953 Soluble
44 Q I 0.0000 Buried
45 K I -1.8575 Soluble
46 P I -2.0387 Soluble
47 D I -2.5856 Soluble
48 Q I -2.6330 Soluble
49 S I -1.7207 Soluble
50 P I 0.0000 Buried
51 K I -1.8609 Soluble
52 L I 0.0000 Buried
53 W I 0.0000 Buried
54 V I 0.0000 Buried
55 H I -0.3190 Soluble
56 G I 0.0219 Aggregation-prone
57 T I -0.2466 Soluble
65 S I -0.7427 Soluble
66 N I -1.3074 Soluble
67 L I -0.4797 Soluble
68 A I 0.0000 Buried
69 S I -0.4377 Soluble
70 G I -0.4611 Soluble
71 V I -0.4799 Soluble
72 P I -0.3374 Soluble
74 S I -0.4880 Soluble
75 R I -1.0544 Soluble
76 F I 0.0000 Buried
77 S I -0.5566 Soluble
78 G I -0.5594 Soluble
79 S I -0.8567 Soluble
80 G I -0.9682 Soluble
83 S I -1.0156 Soluble
84 G I -1.3559 Soluble
85 T I -1.6069 Soluble
86 D I -2.3148 Soluble
87 F I 0.0000 Buried
88 T I -0.6696 Soluble
89 L I 0.0000 Buried
90 T I -0.6565 Soluble
91 I I 0.0000 Buried
92 N I -2.2503 Soluble
93 S I -2.6655 Soluble
94 L I 0.0000 Buried
95 E I -2.7384 Soluble
96 A I -2.0455 Soluble
97 E I -2.3371 Soluble
98 D I 0.0000 Buried
99 A I -1.1694 Soluble
100 A I 0.0000 Buried
101 T I -0.7439 Soluble
102 Y I 0.0000 Buried
103 Y I 0.0000 Buried
104 C I 0.0000 Buried
105 H I 0.0000 Buried
106 H I 0.4791 Aggregation-prone
107 W I 0.2685 Aggregation-prone
108 S I -0.1201 Soluble
109 N I -1.1814 Soluble
114 T I -1.1858 Soluble
115 Q I -0.8582 Soluble
116 W I 0.0000 Buried
117 T I 0.5294 Aggregation-prone
118 F I 0.1888 Aggregation-prone
119 G I 0.0000 Buried
120 G I -0.9632 Soluble
121 G I -1.0272 Soluble
122 T I 0.0000 Buried
123 K I -1.1665 Soluble
124 V I 0.0000 Buried
125 E I -1.1344 Soluble
126 I I 0.0000 Buried
127 K I -1.5741 Soluble
128 R I -1.1551 Soluble
129 T I -0.1392 Soluble
130 V I 0.3867 Aggregation-prone
131 A I 0.1425 Aggregation-prone
132 A I -0.0969 Soluble
133 P I 0.0000 Buried
134 S I -0.1333 Soluble
135 V I 0.0000 Buried
136 F I 0.1060 Aggregation-prone
137 I I -0.0068 Soluble
138 F I 0.0000 Buried
139 P I -0.5178 Soluble
140 P I 0.0000 Buried
141 S I -1.5901 Soluble
142 D I -2.8220 Soluble
143 E I -2.4726 Soluble
144 Q I 0.0000 Buried
145 L I -2.2087 Soluble
146 K I -2.7182 Soluble
147 S I -1.7191 Soluble
148 G I -1.2575 Soluble
149 T I -0.9983 Soluble
150 A I 0.0000 Buried
151 S I 0.0000 Buried
152 V I 0.0000 Buried
153 V I 0.0000 Buried
154 C I 0.0000 Buried
155 L I 0.0000 Buried
156 L I 0.0000 Buried
157 N I 0.0000 Buried
158 N I -0.7056 Soluble
159 F I 0.0000 Buried
160 Y I 0.0000 Buried
161 P I -1.5024 Soluble
162 R I -2.2591 Soluble
163 E I -2.8594 Soluble
164 A I -2.1128 Soluble
165 K I -2.1986 Soluble
166 V I -1.0073 Soluble
167 Q I -0.4486 Soluble
168 W I 0.0000 Buried
169 K I -0.4590 Soluble
170 V I 0.0000 Buried
171 D I -1.6695 Soluble
172 N I -1.4288 Soluble
173 A I -0.2038 Soluble
174 L I 0.7745 Aggregation-prone
175 Q I -0.1203 Soluble
176 S I -0.4091 Soluble
177 G I -1.0706 Soluble
178 N I -1.0419 Soluble
179 S I -1.3882 Soluble
180 Q I -2.0810 Soluble
181 E I -2.0312 Soluble
182 S I -0.5848 Soluble
183 V I -0.3922 Soluble
184 T I 0.0000 Buried
185 E I -2.0432 Soluble
186 Q I -1.7294 Soluble
187 D I -1.7984 Soluble
188 S I -1.9563 Soluble
189 K I -2.2400 Soluble
190 D I -1.7545 Soluble
191 S I -1.7461 Soluble
192 T I 0.0000 Buried
193 Y I 0.0000 Buried
194 S I 0.0000 Buried
195 L I 0.0000 Buried
196 S I 0.0000 Buried
197 S I 0.0000 Buried
198 T I -0.8183 Soluble
199 L I 0.0000 Buried
200 T I -0.5362 Soluble
201 L I -0.6249 Soluble
202 S I -1.0493 Soluble
203 K I -2.0812 Soluble
204 A I -1.7788 Soluble
205 D I -2.3780 Soluble
206 Y I 0.0000 Buried
207 E I -3.1091 Soluble
208 K I -3.3441 Soluble
209 H I -2.7492 Soluble
210 K I -2.6559 Soluble
211 V I -1.1689 Soluble
212 Y I 0.0000 Buried
213 A I 0.0000 Buried
214 C I 0.0000 Buried
215 E I -0.7537 Soluble
216 V I 0.0000 Buried
217 T I -1.1213 Soluble
218 H I 0.0000 Buried
219 Q I -1.6832 Soluble
220 G I -0.4441 Soluble
221 L I -0.2215 Soluble
222 S I -0.4522 Soluble
223 S I -0.4352 Soluble
224 P I -0.5426 Soluble
225 V I -0.0173 Soluble
226 T I -0.4564 Soluble
227 K I -0.7588 Soluble
228 S I -0.6427 Soluble
229 F I 0.0000 Buried
230 N I -1.7778 Soluble
231 R I -2.2863 Soluble
232 G I -1.7741 Soluble
233 E I -2.0415 Soluble
234 C I -0.5187 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7567 3.3338 CSV PDB
4.5 -0.8046 3.3339 CSV PDB
5.0 -0.8622 3.3342 CSV PDB
5.5 -0.9177 3.3351 CSV PDB
6.0 -0.9566 3.3373 CSV PDB
6.5 -0.9664 3.3413 CSV PDB
7.0 -0.9455 3.3459 CSV PDB
7.5 -0.9032 3.3492 CSV PDB
8.0 -0.8497 3.3508 CSV PDB
8.5 -0.7889 3.3515 CSV PDB
9.0 -0.7218 3.3517 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH