Sovipostobart / 5tru

A4DAB246_5tru_h_l | Source PDB: 5tru | Chains: h, l | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6806
Max Score
2.0181
Min Score
-3.7046
Total Score
-287.91
Score CDR
-0.1955
Score non-CDR
-0.7398

Calculation Parameters

Chain Sequence(s) QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) h (Heavy), l (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7046
Maximum Score 2.0181
Average Score -0.6806
Total Score -287.9141

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q h -1.1910 Soluble
2 V h -0.2881 Soluble
3 Q h -0.8331 Soluble
4 L h 0.0000 Buried
5 V h 0.3637 Aggregation-prone
6 E h 0.0000 Buried
7 S h -0.5650 Soluble
8 G h -0.8878 Soluble
9 G h -0.3884 Soluble
11 G h -0.1383 Soluble
12 V h 0.2724 Aggregation-prone
13 V h 0.0000 Buried
14 Q h -2.1193 Soluble
15 P h -2.3811 Soluble
16 G h -2.4063 Soluble
17 R h -2.9160 Soluble
18 S h -2.1002 Soluble
19 L h -1.3611 Soluble
20 R h -1.8381 Soluble
21 L h 0.0000 Buried
22 S h -0.4112 Soluble
23 C h 0.0000 Buried
24 A h -0.2732 Soluble
25 A h 0.0000 Buried
26 S h -0.5502 Soluble
27 G h -0.8076 Soluble
28 F h -0.2322 Soluble
29 T h -0.0773 Soluble
30 F h 0.0000 Buried
35 S h -0.4287 Soluble
36 S h 0.1532 Aggregation-prone
37 Y h 0.5695 Aggregation-prone
38 T h 0.0000 Buried
39 M h 0.0000 Buried
40 H h 0.0000 Buried
41 W h 0.0000 Buried
42 V h 0.0000 Buried
43 R h 0.0000 Buried
44 Q h -0.7589 Soluble
45 A h -1.1214 Soluble
46 P h -1.0651 Soluble
47 G h -1.4802 Soluble
48 K h -2.4133 Soluble
49 G h -1.6373 Soluble
50 L h 0.0000 Buried
51 E h -1.3020 Soluble
52 W h 0.0000 Buried
53 V h 0.0000 Buried
54 T h 0.0000 Buried
55 F h 0.1128 Aggregation-prone
56 I h 0.0000 Buried
57 S h -0.7150 Soluble
58 Y h -0.1876 Soluble
59 D h -2.0358 Soluble
62 G h -1.8957 Soluble
63 N h -2.3717 Soluble
64 N h -2.0694 Soluble
65 K h -0.9145 Soluble
66 Y h -0.0741 Soluble
67 Y h -0.6226 Soluble
68 A h -1.2942 Soluble
69 D h -2.3730 Soluble
70 S h -1.5794 Soluble
71 V h 0.0000 Buried
72 K h -2.4998 Soluble
74 G h -1.7881 Soluble
75 R h -1.3943 Soluble
76 F h 0.0000 Buried
77 T h -0.7735 Soluble
78 I h 0.0000 Buried
79 S h -0.8947 Soluble
80 R h -1.1056 Soluble
81 D h -1.9222 Soluble
82 N h -1.8626 Soluble
83 S h -1.6579 Soluble
84 K h -2.4157 Soluble
85 N h -1.7943 Soluble
86 T h 0.0000 Buried
87 L h 0.0000 Buried
88 Y h -0.4247 Soluble
89 L h 0.0000 Buried
90 Q h -1.0902 Soluble
91 M h 0.0000 Buried
92 N h -1.9130 Soluble
93 S h -1.9541 Soluble
94 L h 0.0000 Buried
95 R h -3.1235 Soluble
96 A h -2.0173 Soluble
97 E h -2.3982 Soluble
98 D h 0.0000 Buried
99 T h -0.7238 Soluble
100 A h 0.0000 Buried
101 I h 0.4852 Aggregation-prone
102 Y h 0.0000 Buried
103 Y h 0.0000 Buried
104 C h 0.0000 Buried
105 A h 0.0000 Buried
106 R h 0.0000 Buried
107 T h 0.6641 Aggregation-prone
108 G h 0.0000 Buried
109 W h 1.6436 Aggregation-prone
110 L h 2.0181 Aggregation-prone
113 G h 0.9649 Aggregation-prone
114 P h 0.0000 Buried
115 F h 0.0000 Buried
116 D h 0.1822 Aggregation-prone
117 Y h 0.2422 Aggregation-prone
118 W h -0.2020 Soluble
119 G h 0.0000 Buried
120 Q h -1.2377 Soluble
121 G h -0.4824 Soluble
122 T h 0.0357 Aggregation-prone
123 L h 0.5331 Aggregation-prone
124 V h 0.0000 Buried
125 T h 0.0000 Buried
126 V h 0.0000 Buried
127 S h -0.9880 Soluble
128 S h -0.8572 Soluble
129 A h -0.4689 Soluble
130 S h -0.5501 Soluble
131 T h -0.5356 Soluble
132 K h -1.1144 Soluble
133 G h -1.2748 Soluble
134 P h -0.5353 Soluble
135 S h -0.4098 Soluble
136 V h 0.0000 Buried
137 F h 0.0000 Buried
138 P h -1.2556 Soluble
139 L h 0.0000 Buried
140 A h -0.8188 Soluble
141 P h 0.0000 Buried
142 S h -0.3454 Soluble
143 S h -0.3621 Soluble
144 T h -0.2834 Soluble
145 A h -0.2025 Soluble
146 A h 0.0000 Buried
147 L h 0.0000 Buried
148 G h 0.0000 Buried
149 C h 0.0000 Buried
150 L h 0.0000 Buried
151 V h 0.0000 Buried
152 K h 0.0000 Buried
153 D h -0.2578 Soluble
154 Y h 0.0000 Buried
155 F h 0.0000 Buried
156 P h 0.0000 Buried
157 E h -0.7087 Soluble
158 P h -0.8759 Soluble
159 V h -0.7399 Soluble
160 T h -0.6304 Soluble
161 V h -0.3264 Soluble
162 S h -0.3561 Soluble
163 W h 0.0000 Buried
164 N h -0.8090 Soluble
165 S h -0.6643 Soluble
166 G h -0.5622 Soluble
167 A h -0.2853 Soluble
168 L h -0.0286 Soluble
169 T h -0.1732 Soluble
170 S h -0.1735 Soluble
171 G h -0.1869 Soluble
172 V h 0.2086 Aggregation-prone
173 H h -0.2351 Soluble
174 T h -0.1136 Soluble
175 F h 0.0000 Buried
176 P h -0.2110 Soluble
177 A h 0.2588 Aggregation-prone
178 V h 0.7229 Aggregation-prone
179 L h 1.4544 Aggregation-prone
180 Q h 0.4559 Aggregation-prone
181 S h -0.0968 Soluble
182 S h -0.1422 Soluble
183 G h 0.1347 Aggregation-prone
184 L h 0.1342 Aggregation-prone
185 Y h 0.0000 Buried
186 S h 0.0000 Buried
187 L h 0.0000 Buried
188 S h 0.0000 Buried
189 S h 0.0000 Buried
190 V h 0.0000 Buried
191 V h 0.0000 Buried
192 T h -0.1129 Soluble
193 V h 0.0000 Buried
194 P h -0.3779 Soluble
195 S h -0.4734 Soluble
196 S h -0.5606 Soluble
197 S h -0.6861 Soluble
198 L h -0.9715 Soluble
199 G h -1.2435 Soluble
200 T h -0.8842 Soluble
201 Q h -1.5649 Soluble
202 T h -1.3150 Soluble
203 Y h 0.0000 Buried
204 I h -1.0237 Soluble
205 C h 0.0000 Buried
206 N h -1.4215 Soluble
207 V h 0.0000 Buried
208 N h -1.8481 Soluble
209 H h 0.0000 Buried
210 K h -2.8484 Soluble
211 P h -1.6046 Soluble
212 S h -1.8165 Soluble
213 N h -2.6427 Soluble
214 T h -2.0893 Soluble
215 K h -2.6203 Soluble
216 V h -1.3831 Soluble
217 D h -2.4120 Soluble
218 K h -2.0227 Soluble
219 R h -2.6309 Soluble
220 V h 0.0000 Buried
221 E h -2.5377 Soluble
222 P h -1.2591 Soluble
1 E l -1.5641 Soluble
2 I l -0.4398 Soluble
3 V l 0.3873 Aggregation-prone
4 L l 0.0000 Buried
5 T l -0.9093 Soluble
6 Q l -1.1457 Soluble
7 S l -0.9643 Soluble
8 P l -0.5883 Soluble
9 G l -0.9543 Soluble
10 T l -0.5062 Soluble
11 L l -0.2527 Soluble
12 S l -0.5679 Soluble
13 L l -0.9551 Soluble
14 S l -1.4608 Soluble
15 P l -1.7728 Soluble
16 G l -2.1569 Soluble
17 E l -3.0324 Soluble
18 R l -3.1629 Soluble
19 A l 0.0000 Buried
20 T l -0.6775 Soluble
21 L l 0.0000 Buried
22 S l -0.9064 Soluble
23 C l 0.0000 Buried
24 R l -2.4714 Soluble
25 A l -1.2839 Soluble
26 S l -1.0877 Soluble
27 Q l -1.8014 Soluble
28 S l -1.4642 Soluble
29 V l 0.0000 Buried
30 G l -0.7695 Soluble
36 S l -0.3876 Soluble
37 S l -0.0422 Soluble
38 Y l 0.7511 Aggregation-prone
39 L l 0.0000 Buried
40 A l 0.0000 Buried
41 W l 0.0000 Buried
42 Y l 0.0000 Buried
43 Q l 0.0000 Buried
44 Q l 0.0000 Buried
45 K l -1.4793 Soluble
46 P l -1.3209 Soluble
47 G l -1.2849 Soluble
48 Q l -1.9405 Soluble
49 A l -1.2894 Soluble
50 P l 0.0000 Buried
51 R l -1.7658 Soluble
52 L l 0.0000 Buried
53 L l 0.0000 Buried
54 I l 0.0000 Buried
55 Y l 0.6829 Aggregation-prone
56 G l 0.7763 Aggregation-prone
57 A l 0.0000 Buried
65 F l 1.5244 Aggregation-prone
66 S l 0.2248 Aggregation-prone
67 R l -1.1520 Soluble
68 A l 0.0000 Buried
69 T l -0.5712 Soluble
70 G l -0.8196 Soluble
71 I l -0.9953 Soluble
72 P l -1.2633 Soluble
74 D l -2.2113 Soluble
75 R l -1.8948 Soluble
76 F l 0.0000 Buried
77 S l -0.4028 Soluble
78 G l 0.3923 Aggregation-prone
79 S l -0.0524 Soluble
80 G l -0.7642 Soluble
83 S l -0.6567 Soluble
84 G l -1.2726 Soluble
85 T l -1.8853 Soluble
86 D l -2.4025 Soluble
87 F l 0.0000 Buried
88 T l -0.5479 Soluble
89 L l 0.0000 Buried
90 T l -0.8074 Soluble
91 I l 0.0000 Buried
92 S l -2.4235 Soluble
93 R l -3.1972 Soluble
94 L l 0.0000 Buried
95 E l -1.6673 Soluble
96 P l -1.3826 Soluble
97 E l -1.9336 Soluble
98 D l 0.0000 Buried
99 F l -0.5029 Soluble
100 A l 0.0000 Buried
101 V l -0.2268 Soluble
102 Y l 0.0000 Buried
103 Y l 0.0000 Buried
104 C l 0.0000 Buried
105 Q l 0.0000 Buried
106 Q l 0.0000 Buried
107 Y l 0.0000 Buried
108 G l -0.3156 Soluble
109 S l -0.6153 Soluble
114 S l -0.4543 Soluble
115 P l -0.5186 Soluble
116 W l -0.1419 Soluble
117 T l -0.2361 Soluble
118 F l -0.0587 Soluble
119 G l 0.0000 Buried
120 Q l -1.6875 Soluble
121 G l -1.1923 Soluble
122 T l 0.0000 Buried
123 K l -1.0058 Soluble
124 V l 0.0000 Buried
125 E l 0.0000 Buried
126 I l -0.4900 Soluble
127 K l -1.2693 Soluble
128 R l -0.7970 Soluble
129 T l 0.2134 Aggregation-prone
130 V l 1.0874 Aggregation-prone
131 A l 0.4722 Aggregation-prone
132 A l 0.0748 Aggregation-prone
133 P l 0.0000 Buried
134 S l -0.1235 Soluble
135 V l 0.0000 Buried
136 F l -0.0085 Soluble
137 I l 0.0000 Buried
138 F l 0.0000 Buried
139 P l -0.5267 Soluble
140 P l 0.0000 Buried
141 S l -1.7080 Soluble
142 D l -3.0809 Soluble
143 E l -3.2725 Soluble
144 Q l 0.0000 Buried
145 L l -2.3969 Soluble
146 K l -2.8962 Soluble
147 S l -1.8582 Soluble
148 G l -1.1347 Soluble
149 T l -0.8563 Soluble
150 A l 0.0000 Buried
151 S l 0.0000 Buried
152 V l 0.0000 Buried
153 V l 0.0000 Buried
154 C l 0.0000 Buried
155 L l 0.0000 Buried
156 L l 0.0000 Buried
157 N l 0.0000 Buried
158 N l -0.7060 Soluble
159 F l 0.0000 Buried
160 Y l 0.0000 Buried
161 P l -1.0002 Soluble
162 R l -1.6767 Soluble
163 E l -2.5937 Soluble
164 A l -2.0484 Soluble
165 K l -2.2062 Soluble
166 V l -1.1292 Soluble
167 Q l -0.4549 Soluble
168 W l 0.0000 Buried
169 K l -0.5636 Soluble
170 V l -0.8737 Soluble
171 D l -1.8626 Soluble
173 A l -0.0161 Soluble
174 L l 0.8522 Aggregation-prone
175 Q l -0.1252 Soluble
176 S l -0.4936 Soluble
177 G l -0.8475 Soluble
178 N l -0.6728 Soluble
179 S l -0.8313 Soluble
180 Q l -0.9336 Soluble
181 E l -1.4661 Soluble
182 S l -0.6874 Soluble
183 V l -0.5555 Soluble
184 T l -0.9278 Soluble
185 E l -2.0814 Soluble
186 Q l -1.6565 Soluble
187 D l -1.8328 Soluble
188 S l -1.7064 Soluble
189 K l -2.1967 Soluble
190 D l -1.6652 Soluble
191 S l -1.6162 Soluble
192 T l 0.0000 Buried
193 Y l 0.0000 Buried
194 S l 0.0000 Buried
195 L l 0.0000 Buried
196 S l 0.0000 Buried
197 S l 0.0000 Buried
198 T l -0.5291 Soluble
199 L l 0.0000 Buried
200 T l -0.2104 Soluble
201 L l -0.3925 Soluble
202 S l -1.1260 Soluble
203 K l -1.8086 Soluble
204 A l -2.0030 Soluble
205 D l -2.4940 Soluble
206 Y l -2.4568 Soluble
207 E l -3.7046 Soluble
208 K l -3.5078 Soluble
209 H l -3.2407 Soluble
210 K l -3.2042 Soluble
211 V l -1.6450 Soluble
212 Y l 0.0000 Buried
213 A l 0.0000 Buried
214 C l 0.0000 Buried
215 E l -0.9086 Soluble
216 V l 0.0000 Buried
217 T l -1.0950 Soluble
218 H l 0.0000 Buried
219 Q l -1.5408 Soluble
220 G l -0.3293 Soluble
221 L l -0.2063 Soluble
222 S l -0.4948 Soluble
223 S l -0.3703 Soluble
224 P l -0.4698 Soluble
225 V l 0.0799 Aggregation-prone
226 T l -0.4846 Soluble
227 K l -0.5824 Soluble
228 S l -0.1867 Soluble
229 F l -0.2043 Soluble
230 N l -1.8760 Soluble
231 R l -2.7499 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6785 3.5232 CSV PDB
4.5 -0.7246 3.5167 CSV PDB
5.0 -0.7786 3.5101 CSV PDB
5.5 -0.8314 3.5034 CSV PDB
6.0 -0.873 3.4968 CSV PDB
6.5 -0.8944 3.4902 CSV PDB
7.0 -0.8941 3.4838 CSV PDB
7.5 -0.8786 3.4781 CSV PDB
8.0 -0.8541 3.4738 CSV PDB
8.5 -0.8227 3.4712 CSV PDB
9.0 -0.7848 3.4699 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH