Melredableukin / 6ghg

A4DAB168_6ghg_H_L | Source PDB: 6ghg | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7021
Max Score
1.3183
Min Score
-3.8371
Total Score
-299.11
Score CDR
-0.262
Score non-CDR
-0.7516

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGSGFDYWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVEDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDEKVEPKSEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPLTFGQGTKVEIKRTVAAPSVFIFPPSDKKLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8371
Maximum Score 1.3183
Average Score -0.7021
Total Score -299.1109

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9958 Soluble
2 V H -1.0690 Soluble
3 Q H -0.8957 Soluble
4 L H 0.0000 Buried
5 L H 0.4630 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.3553 Soluble
8 G H -0.6669 Soluble
9 G H 0.1088 Aggregation-prone
11 G H 0.6868 Aggregation-prone
12 L H 1.0522 Aggregation-prone
13 V H -0.1603 Soluble
14 Q H -1.3621 Soluble
15 P H -1.7170 Soluble
16 G H -1.3855 Soluble
17 G H -1.0052 Soluble
18 S H -0.9723 Soluble
19 L H -0.7817 Soluble
20 R H -1.4910 Soluble
21 L H 0.0000 Buried
22 S H -0.3248 Soluble
23 C H 0.0000 Buried
24 A H -0.2581 Soluble
25 A H 0.0000 Buried
26 S H -0.9666 Soluble
27 G H -1.1045 Soluble
28 F H -0.3805 Soluble
29 T H -0.1810 Soluble
30 F H 0.0000 Buried
35 S H -0.5437 Soluble
36 S H -0.1400 Soluble
37 Y H 0.2945 Aggregation-prone
38 A H 0.0255 Aggregation-prone
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.6774 Soluble
45 A H -1.0588 Soluble
46 P H -0.8131 Soluble
47 G H -1.5264 Soluble
48 K H -2.3835 Soluble
49 G H -1.5973 Soluble
50 L H 0.0000 Buried
51 E H -1.1164 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 A H 0.0000 Buried
56 I H 0.0000 Buried
57 S H -0.2362 Soluble
58 G H -0.3679 Soluble
59 S H -0.6644 Soluble
62 G H -0.7690 Soluble
63 G H -0.7033 Soluble
64 S H -0.3042 Soluble
65 T H 0.0736 Aggregation-prone
66 Y H 0.1724 Aggregation-prone
67 Y H -0.5415 Soluble
68 A H -1.4011 Soluble
69 D H -2.5546 Soluble
70 S H -1.7337 Soluble
71 V H 0.0000 Buried
72 K H -2.4160 Soluble
74 G H -1.6244 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.5921 Soluble
78 I H 0.0000 Buried
79 S H -0.4300 Soluble
80 R H -1.0345 Soluble
81 D H -1.5484 Soluble
82 N H -1.7294 Soluble
83 S H -1.5751 Soluble
84 K H -2.3727 Soluble
85 N H -1.7398 Soluble
86 T H -1.0321 Soluble
87 L H 0.0000 Buried
88 Y H -0.3595 Soluble
89 L H 0.0000 Buried
90 Q H -0.8633 Soluble
91 M H 0.0000 Buried
92 N H -1.2795 Soluble
93 S H -1.2230 Soluble
94 L H 0.0000 Buried
95 R H -2.2897 Soluble
96 A H -1.6970 Soluble
97 E H -2.2242 Soluble
98 D H 0.0000 Buried
99 T H -0.3873 Soluble
100 A H 0.0000 Buried
101 V H 0.7572 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 K H 0.0000 Buried
107 G H -0.1260 Soluble
108 S H -0.0406 Soluble
114 G H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -1.1319 Soluble
117 Y H -0.3777 Soluble
118 W H -0.3412 Soluble
119 G H 0.0000 Buried
120 Q H -1.0576 Soluble
121 G H 0.0000 Buried
122 T H 0.4255 Aggregation-prone
123 L H 1.3183 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.8182 Soluble
128 S H -0.7432 Soluble
129 A H -0.4306 Soluble
130 S H -0.4807 Soluble
131 T H -0.7164 Soluble
132 K H -1.2920 Soluble
133 G H -1.5397 Soluble
134 P H -0.9343 Soluble
135 S H -0.5387 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9787 Soluble
139 L H 0.0000 Buried
140 A H -1.0929 Soluble
141 P H -0.7045 Soluble
142 S H -0.6156 Soluble
143 G H -0.6592 Soluble
144 T H -0.4979 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 E H -0.5748 Soluble
153 D H -1.2202 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.7341 Soluble
157 E H -1.1571 Soluble
158 P H -1.1282 Soluble
159 V H -0.9100 Soluble
160 T H -0.6867 Soluble
161 V H -0.3510 Soluble
162 S H -0.3394 Soluble
163 W H 0.0000 Buried
164 N H -0.6611 Soluble
165 S H -0.5796 Soluble
166 G H -0.3599 Soluble
167 A H -0.1668 Soluble
168 L H 0.0328 Aggregation-prone
169 T H -0.1434 Soluble
170 S H -0.1964 Soluble
171 G H -0.3029 Soluble
172 V H 0.1795 Aggregation-prone
173 H H -0.3351 Soluble
174 T H -0.1763 Soluble
175 F H 0.0000 Buried
176 P H -0.2279 Soluble
177 A H 0.3026 Aggregation-prone
178 V H 0.7701 Aggregation-prone
179 L H 1.1196 Aggregation-prone
180 Q H 0.0195 Aggregation-prone
181 S H -0.1764 Soluble
182 S H -0.4071 Soluble
183 G H -0.1089 Soluble
184 L H -0.3442 Soluble
185 Y H 0.0274 Aggregation-prone
186 S H 0.0177 Aggregation-prone
187 L H 0.2640 Aggregation-prone
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1341 Soluble
193 V H 0.0000 Buried
194 P H -0.6016 Soluble
195 S H -0.5530 Soluble
196 S H -0.6271 Soluble
197 S H -0.6596 Soluble
198 L H -0.8463 Soluble
199 G H -0.9830 Soluble
200 T H -0.7296 Soluble
201 Q H -1.2696 Soluble
202 T H -1.0956 Soluble
203 Y H 0.0000 Buried
204 I H -1.2386 Soluble
205 C H 0.0000 Buried
206 N H -1.4188 Soluble
207 V H 0.0000 Buried
208 N H -1.9898 Soluble
209 H H 0.0000 Buried
210 K H -2.8072 Soluble
211 P H -1.7086 Soluble
212 S H -1.8463 Soluble
213 N H -2.5370 Soluble
214 T H -2.0577 Soluble
215 K H -2.6516 Soluble
216 V H -1.4599 Soluble
217 D H -2.4850 Soluble
218 E H -1.9275 Soluble
219 K H -2.3413 Soluble
220 V H 0.0000 Buried
221 E H -2.8017 Soluble
222 P H -1.8129 Soluble
223 K H -2.1694 Soluble
224 S H -1.0913 Soluble
1 E L -1.7046 Soluble
2 I L -0.3990 Soluble
3 V L 0.8167 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.5635 Soluble
6 Q L 0.0000 Buried
7 S L -0.7752 Soluble
8 P L -0.5018 Soluble
9 G L -0.8424 Soluble
10 T L -0.5737 Soluble
11 L L -0.1389 Soluble
12 S L -0.3944 Soluble
13 L L -0.6309 Soluble
14 S L -1.1586 Soluble
15 P L -1.7005 Soluble
16 G L -2.0411 Soluble
17 E L -2.4508 Soluble
18 R L -2.9356 Soluble
19 A L 0.0000 Buried
20 T L -0.6186 Soluble
21 L L 0.0000 Buried
22 S L -0.7764 Soluble
23 C L 0.0000 Buried
24 R L -1.8211 Soluble
25 A L 0.0000 Buried
26 S L -0.8668 Soluble
27 Q L -1.6313 Soluble
28 S L -1.3070 Soluble
29 V L 0.0000 Buried
30 S L -0.4321 Soluble
36 S L -0.2111 Soluble
37 S L -0.1504 Soluble
38 Y L 0.6200 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.2102 Soluble
45 K L -1.6410 Soluble
46 P L -1.3688 Soluble
47 G L -1.4793 Soluble
48 Q L -2.2113 Soluble
49 A L -1.4718 Soluble
50 P L 0.0000 Buried
51 R L -2.2075 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.2927 Aggregation-prone
56 G L 0.0671 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.5117 Soluble
66 S L -0.5861 Soluble
67 R L -1.4247 Soluble
68 A L -0.9440 Soluble
69 T L -0.6720 Soluble
70 G L -0.7920 Soluble
71 I L -1.0267 Soluble
72 P L -1.2446 Soluble
74 D L -2.1818 Soluble
75 R L -2.1040 Soluble
76 F L 0.0000 Buried
77 S L -0.9359 Soluble
78 G L -0.5072 Soluble
79 S L -0.7299 Soluble
80 G L -1.0977 Soluble
83 S L -0.9886 Soluble
84 G L -1.1179 Soluble
85 T L -1.6128 Soluble
86 D L -2.0493 Soluble
87 F L 0.0000 Buried
88 T L -0.7226 Soluble
89 L L 0.0000 Buried
90 T L -0.8260 Soluble
91 I L 0.0000 Buried
92 S L -2.3595 Soluble
93 R L -3.2073 Soluble
94 L L 0.0000 Buried
95 E L -2.4326 Soluble
96 P L -1.5665 Soluble
97 E L -2.3276 Soluble
98 D L 0.0000 Buried
99 F L -0.5585 Soluble
100 A L 0.0000 Buried
101 V L -0.1211 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.2607 Aggregation-prone
108 G L -0.0429 Soluble
109 S L -0.3655 Soluble
114 S L -0.4080 Soluble
115 P L -0.4943 Soluble
116 L L 0.0480 Aggregation-prone
117 T L -0.0300 Soluble
118 F L 0.0569 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.3938 Soluble
121 G L -1.0043 Soluble
122 T L 0.0000 Buried
123 K L -0.4153 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.1836 Soluble
127 K L -0.9692 Soluble
128 R L -0.8950 Soluble
129 T L -0.0972 Soluble
130 V L 0.4071 Aggregation-prone
131 A L 0.1643 Aggregation-prone
132 A L -0.0829 Soluble
133 P L 0.0000 Buried
134 S L -0.1474 Soluble
135 V L 0.0000 Buried
136 F L 0.0706 Aggregation-prone
137 I L 0.0784 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.7294 Soluble
140 P L -0.9882 Soluble
141 S L -1.6612 Soluble
142 D L -2.7547 Soluble
143 K L -2.4323 Soluble
144 K L 0.0000 Buried
145 L L -2.0720 Soluble
146 K L -2.7063 Soluble
147 S L -1.6638 Soluble
148 G L -1.2168 Soluble
149 T L -1.0573 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8073 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6617 Soluble
162 R L -2.8107 Soluble
163 E L -3.0481 Soluble
164 A L -2.0909 Soluble
165 K L -1.9964 Soluble
166 V L -0.8738 Soluble
167 Q L -0.3685 Soluble
168 W L 0.0000 Buried
169 K L -0.4531 Soluble
170 V L 0.0000 Buried
171 D L -1.9371 Soluble
172 N L -1.4997 Soluble
173 A L -0.2286 Soluble
174 L L 0.7949 Aggregation-prone
175 Q L -0.0164 Soluble
176 S L -0.3691 Soluble
177 G L -0.8912 Soluble
178 N L -0.8011 Soluble
179 S L -1.1263 Soluble
180 Q L -1.6122 Soluble
181 E L -1.9835 Soluble
182 S L -0.8595 Soluble
183 V L -0.9070 Soluble
184 T L -1.2209 Soluble
185 E L -2.2937 Soluble
186 Q L -1.8733 Soluble
187 D L -2.0274 Soluble
188 S L -1.6961 Soluble
189 K L -2.2641 Soluble
190 D L -1.7348 Soluble
191 S L -1.6191 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7021 Soluble
199 L L 0.0000 Buried
200 T L -0.5367 Soluble
201 L L -0.8362 Soluble
202 S L -1.2312 Soluble
203 K L -2.1904 Soluble
204 A L -2.0897 Soluble
205 D L -3.0908 Soluble
206 Y L 0.0000 Buried
207 E L -3.7241 Soluble
208 K L -3.8371 Soluble
209 H L -3.1917 Soluble
210 K L -3.3175 Soluble
211 V L -1.4314 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6586 Soluble
216 V L 0.0000 Buried
217 T L -1.0734 Soluble
218 H L 0.0000 Buried
219 Q L -1.6753 Soluble
220 G L -0.3900 Soluble
221 L L -0.2205 Soluble
222 S L -0.4574 Soluble
223 S L -0.4106 Soluble
224 P L -0.4924 Soluble
225 V L -0.0402 Soluble
226 T L -0.5149 Soluble
227 K L -0.8280 Soluble
228 S L -0.6305 Soluble
229 F L -0.9050 Soluble
230 N L -1.9046 Soluble
231 R L -2.5824 Soluble
232 G L -2.2684 Soluble
233 E L -2.1993 Soluble
234 C L -0.8486 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7099 2.2496 CSV PDB
4.5 -0.7546 2.22 CSV PDB
5.0 -0.8091 2.1738 CSV PDB
5.5 -0.8638 2.1188 CSV PDB
6.0 -0.907 2.0641 CSV PDB
6.5 -0.9288 2.0176 CSV PDB
7.0 -0.9271 1.9865 CSV PDB
7.5 -0.9086 1.9712 CSV PDB
8.0 -0.8802 1.9653 CSV PDB
8.5 -0.8446 1.9633 CSV PDB
9.0 -0.802 2.0049 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH