Amubarvimab / 7e8m

A4DAB014_7e8m_H_L | Source PDB: 7e8m | Chains: H, L | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6793
Max Score
1.7482
Min Score
-3.6113
Total Score
-287.34
Score CDR
-0.0723
Score non-CDR
-0.7498

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGITVSSNYMNWVRQAPGKGLEWVSLIYSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYHCARDLVVYGMDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPEIVLTQSPGTLSISPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6113
Maximum Score 1.7482
Average Score -0.6793
Total Score -287.3411

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.9739 Aggregation-prone
3 Q H -0.3707 Soluble
4 L H 0.0000 Buried
5 V H 0.3247 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4085 Soluble
8 G H -0.7827 Soluble
9 G H -0.4217 Soluble
11 G H 0.0354 Aggregation-prone
12 L H 0.6532 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.5253 Soluble
15 P H -1.7588 Soluble
16 G H -1.5331 Soluble
17 G H -1.1219 Soluble
18 S H -1.3104 Soluble
19 L H -0.9545 Soluble
20 R H -1.7968 Soluble
21 L H 0.0000 Buried
22 S H -0.5506 Soluble
23 C H 0.0000 Buried
24 A H -0.4106 Soluble
25 A H -0.3156 Soluble
26 S H -0.1523 Soluble
27 G H -0.1062 Soluble
28 I H 0.0000 Buried
29 T H -0.8219 Soluble
30 V H 0.0000 Buried
35 S H -0.8933 Soluble
36 S H -0.5369 Soluble
37 N H -0.1038 Soluble
38 Y H 0.2884 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8279 Soluble
45 A H -1.2325 Soluble
46 P H -1.1905 Soluble
47 G H -1.4719 Soluble
48 K H -2.3991 Soluble
49 G H -1.6075 Soluble
50 L H 0.0000 Buried
51 E H -1.0518 Soluble
52 W H -0.1894 Soluble
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 L H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H 0.0793 Aggregation-prone
58 S H -0.3494 Soluble
59 G H -0.5986 Soluble
63 G H -0.5346 Soluble
64 S H -0.1477 Soluble
65 T H 0.2042 Aggregation-prone
66 Y H 0.2056 Aggregation-prone
67 Y H -0.6201 Soluble
68 A H -1.2693 Soluble
69 D H -2.3780 Soluble
70 S H -1.6803 Soluble
71 V H 0.0000 Buried
72 K H -2.4857 Soluble
74 G H -1.7564 Soluble
75 R H -1.5328 Soluble
76 F H 0.0000 Buried
77 T H -0.7503 Soluble
78 I H 0.0000 Buried
79 S H -0.6737 Soluble
80 R H -1.4649 Soluble
81 D H -2.1287 Soluble
82 N H -2.4921 Soluble
83 S H -1.9837 Soluble
84 K H -2.6061 Soluble
85 N H -2.0276 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.1488 Soluble
91 M H 0.0000 Buried
92 N H -1.6135 Soluble
93 S H -1.4097 Soluble
94 L H 0.0000 Buried
95 R H -2.5225 Soluble
96 A H -1.8373 Soluble
97 E H -2.3051 Soluble
98 D H 0.0000 Buried
99 T H -0.7426 Soluble
100 A H 0.0000 Buried
101 V H 0.1597 Aggregation-prone
102 Y H 0.0000 Buried
103 H H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.2496 Soluble
107 D H 0.0000 Buried
108 L H 1.2417 Aggregation-prone
109 V H 1.2200 Aggregation-prone
110 V H 1.6509 Aggregation-prone
113 Y H 1.7482 Aggregation-prone
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.6804 Soluble
117 V H -0.4408 Soluble
118 W H -0.5756 Soluble
119 G H 0.0000 Buried
120 Q H -1.3466 Soluble
121 G H 0.0000 Buried
122 T H -0.2233 Soluble
123 T H -0.0682 Soluble
124 V H 0.0000 Buried
125 T H -0.3705 Soluble
126 V H 0.0000 Buried
127 S H -0.9247 Soluble
128 S H -0.7941 Soluble
129 A H -0.5318 Soluble
130 S H -0.7646 Soluble
131 T H -0.9954 Soluble
132 K H -1.7085 Soluble
133 G H -1.6453 Soluble
134 P H 0.0000 Buried
135 S H -0.5189 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0924 Soluble
139 L H 0.0000 Buried
140 A H -0.7949 Soluble
141 P H 0.0000 Buried
142 S H -0.3064 Soluble
144 T H -0.2849 Soluble
145 A H -0.1754 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.5741 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -1.0493 Soluble
157 E H -1.3621 Soluble
158 P H -1.2207 Soluble
159 V H -0.9227 Soluble
160 T H -0.6918 Soluble
161 V H -0.4035 Soluble
162 S H -0.4080 Soluble
163 W H 0.0000 Buried
164 N H -0.7596 Soluble
165 S H -0.6814 Soluble
166 G H -0.5746 Soluble
167 A H -0.2489 Soluble
168 L H 0.0010 Aggregation-prone
169 T H -0.1583 Soluble
170 S H -0.1591 Soluble
171 G H -0.1628 Soluble
172 V H 0.1801 Aggregation-prone
173 H H -0.2698 Soluble
174 T H 0.0544 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.2903 Soluble
177 A H 0.0475 Aggregation-prone
178 V H 0.3493 Aggregation-prone
179 L H 0.8946 Aggregation-prone
180 Q H 0.2054 Aggregation-prone
181 S H -0.0730 Soluble
182 S H -0.2311 Soluble
183 G H 0.0070 Aggregation-prone
184 L H -0.1675 Soluble
185 Y H 0.0886 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1467 Soluble
193 V H 0.0000 Buried
194 P H -0.4165 Soluble
195 S H -0.4741 Soluble
196 S H -0.5771 Soluble
197 S H -0.6198 Soluble
198 L H -0.8235 Soluble
199 G H -0.9757 Soluble
200 T H -0.7166 Soluble
201 Q H -1.1612 Soluble
202 T H -1.0336 Soluble
203 Y H 0.0000 Buried
204 I H -1.2154 Soluble
205 C H 0.0000 Buried
206 N H -1.5283 Soluble
207 V H 0.0000 Buried
208 N H -2.0143 Soluble
209 H H 0.0000 Buried
210 K H -2.8932 Soluble
211 P H -1.8655 Soluble
212 S H -1.9976 Soluble
213 N H -2.7011 Soluble
214 T H -2.1776 Soluble
215 K H -2.7451 Soluble
216 V H -1.6507 Soluble
217 D H -2.5661 Soluble
218 K H -2.1987 Soluble
219 K H -2.2604 Soluble
220 V H 0.0000 Buried
221 E H -2.4042 Soluble
222 P H -1.2016 Soluble
1 E L -1.4655 Soluble
2 I L -0.2130 Soluble
3 V L 0.8634 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.7382 Soluble
6 Q L 0.0000 Buried
7 S L -0.9002 Soluble
8 P L -0.4999 Soluble
9 G L -0.8161 Soluble
10 T L -0.4992 Soluble
11 L L -0.1434 Soluble
12 S L -0.4468 Soluble
13 I L 0.0000 Buried
14 S L -1.3609 Soluble
15 P L -1.7875 Soluble
16 G L -2.0375 Soluble
17 E L -2.6245 Soluble
18 R L -2.9997 Soluble
19 A L 0.0000 Buried
20 T L -0.5312 Soluble
21 L L 0.0000 Buried
22 S L -0.9349 Soluble
23 C L 0.0000 Buried
24 R L -2.2540 Soluble
25 A L 0.0000 Buried
26 S L -0.8947 Soluble
27 Q L -1.5717 Soluble
28 S L -1.1429 Soluble
29 V L -0.4324 Soluble
30 S L -0.3296 Soluble
36 S L -0.2848 Soluble
37 S L 0.4000 Aggregation-prone
38 Y L 0.8992 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.1534 Soluble
45 K L -1.5123 Soluble
46 P L -1.1880 Soluble
47 G L -1.4270 Soluble
48 Q L -2.1657 Soluble
49 A L -1.5820 Soluble
50 P L 0.0000 Buried
51 R L -2.1758 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.5530 Aggregation-prone
56 G L 0.3502 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.6639 Soluble
66 S L -0.4638 Soluble
67 R L -1.1961 Soluble
68 A L -0.6715 Soluble
69 T L -0.5636 Soluble
70 G L -0.7867 Soluble
71 I L -0.9793 Soluble
72 P L -1.2312 Soluble
74 D L -2.3391 Soluble
75 R L -2.0666 Soluble
76 F L 0.0000 Buried
77 S L -0.8828 Soluble
78 G L -0.5309 Soluble
79 S L -0.7145 Soluble
80 G L -1.1077 Soluble
83 S L -1.0318 Soluble
84 G L -1.0961 Soluble
85 T L -1.6585 Soluble
86 D L -2.0748 Soluble
87 F L 0.0000 Buried
88 T L -0.7977 Soluble
89 L L 0.0000 Buried
90 T L -0.8044 Soluble
91 I L 0.0000 Buried
92 S L -2.4015 Soluble
93 R L -3.3655 Soluble
94 L L 0.0000 Buried
95 E L -2.3708 Soluble
96 P L -1.5960 Soluble
97 E L -2.3387 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 V L -0.0993 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 G L -0.5888 Soluble
114 S L -0.4980 Soluble
115 S L -0.2281 Soluble
116 P L 0.0000 Buried
117 T L -0.0613 Soluble
118 F L 0.0641 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.4799 Soluble
121 G L -1.0252 Soluble
122 T L 0.0000 Buried
123 K L -0.5384 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.4062 Soluble
127 K L -1.0471 Soluble
128 R L -0.8981 Soluble
129 T L 0.0566 Aggregation-prone
130 V L 0.7697 Aggregation-prone
131 A L 0.2072 Aggregation-prone
132 A L 0.0002 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.0577 Soluble
135 V L 0.0000 Buried
136 F L 0.0798 Aggregation-prone
137 I L 0.0659 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5344 Soluble
140 P L -0.8145 Soluble
141 S L -1.6312 Soluble
142 D L -2.9413 Soluble
143 E L -3.0081 Soluble
144 Q L 0.0000 Buried
145 L L -2.2025 Soluble
146 K L -2.8006 Soluble
147 S L -1.7162 Soluble
148 G L -1.2937 Soluble
149 T L -0.9672 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.2929 Soluble
158 N L -0.7351 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3359 Soluble
162 R L -2.4295 Soluble
163 E L -2.9850 Soluble
164 A L -2.2288 Soluble
165 K L -2.2357 Soluble
166 V L -1.0327 Soluble
167 Q L -0.4524 Soluble
168 W L 0.0000 Buried
169 K L -0.4132 Soluble
170 V L 0.0000 Buried
171 D L -1.8706 Soluble
172 N L -1.4807 Soluble
173 A L -0.1944 Soluble
174 L L 0.7687 Aggregation-prone
175 Q L -0.0318 Soluble
176 S L -0.3557 Soluble
177 G L -0.9068 Soluble
178 N L -0.8269 Soluble
179 S L -1.1602 Soluble
180 Q L -1.5133 Soluble
181 E L -1.9206 Soluble
182 S L -0.8685 Soluble
183 V L -0.3717 Soluble
184 T L -0.9527 Soluble
185 E L -2.1372 Soluble
186 Q L -1.7484 Soluble
187 D L -2.0024 Soluble
188 S L -1.7222 Soluble
189 K L -2.2641 Soluble
190 D L -1.6712 Soluble
191 S L -1.6333 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6802 Soluble
199 L L 0.0000 Buried
200 T L -0.4383 Soluble
201 L L -0.6622 Soluble
202 S L -1.0173 Soluble
203 K L -1.9642 Soluble
204 A L -1.7932 Soluble
205 D L -2.5254 Soluble
206 Y L 0.0000 Buried
207 E L -3.4697 Soluble
208 K L -3.6113 Soluble
209 H L -3.1490 Soluble
210 K L -3.2685 Soluble
211 V L -1.3952 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6669 Soluble
216 V L 0.0000 Buried
217 T L -1.2128 Soluble
218 H L 0.0000 Buried
219 Q L -1.4324 Soluble
220 G L -0.3098 Soluble
221 L L -0.1992 Soluble
222 S L -0.4662 Soluble
223 S L -0.4094 Soluble
224 P L -0.5289 Soluble
225 V L 0.0693 Aggregation-prone
226 T L -0.4110 Soluble
227 K L -0.6546 Soluble
228 S L -0.5125 Soluble
229 F L -0.8239 Soluble
230 N L -1.8084 Soluble
231 R L -2.4811 Soluble
232 G L -1.9920 Soluble
233 E L -2.1640 Soluble
234 C L -0.4247 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6906 1.712 CSV PDB
4.5 -0.7347 1.6751 CSV PDB
5.0 -0.7876 1.6376 CSV PDB
5.5 -0.8399 1.5999 CSV PDB
6.0 -0.8806 1.5624 CSV PDB
6.5 -0.9001 1.5254 CSV PDB
7.0 -0.8965 1.49 CSV PDB
7.5 -0.8765 1.4695 CSV PDB
8.0 -0.8472 1.469 CSV PDB
8.5 -0.8111 1.4671 CSV PDB
9.0 -0.7692 1.4614 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH