Rozibafusp / 6x4t

A4DAB230_6x4t_D_F | Source PDB: 6x4t | Chains: D, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7036
Max Score
2.5953
Min Score
-3.866
Total Score
-306.06
Score CDR
-0.1875
Score non-CDR
-0.7676

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVAYIKQDGNEKYYVDSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAREGILWFGDLPTFWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIQMTQSPSSLSASVGDRVTITCRASQGISNWLAWYQQKPEKAPKSLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYDSYPRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) D (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.866
Maximum Score 2.5953
Average Score -0.7036
Total Score -306.0577

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.0701 Soluble
2 V D -1.1521 Soluble
3 Q D -1.3751 Soluble
4 L D 0.0000 Buried
5 V D -0.0064 Soluble
6 E D -0.3550 Soluble
7 S D -0.5518 Soluble
8 G D -0.9649 Soluble
9 G D -0.5640 Soluble
11 G D 0.0102 Aggregation-prone
12 L D 0.0546 Aggregation-prone
13 V D -0.6252 Soluble
14 Q D -1.6547 Soluble
15 P D -1.8645 Soluble
16 G D -1.6192 Soluble
17 G D -1.1977 Soluble
18 S D -1.1937 Soluble
19 L D -1.1102 Soluble
20 R D -2.1369 Soluble
21 L D 0.0000 Buried
22 S D -0.5987 Soluble
23 C D 0.0000 Buried
24 A D -0.3578 Soluble
25 A D -0.6648 Soluble
26 S D -0.8648 Soluble
27 G D -1.2055 Soluble
28 F D -0.5391 Soluble
29 T D -0.2115 Soluble
30 F D 0.0000 Buried
35 S D -0.9378 Soluble
36 S D -0.1334 Soluble
37 Y D 0.2900 Aggregation-prone
38 W D 0.0000 Buried
39 M D 0.0000 Buried
40 S D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.8292 Soluble
45 A D -1.2384 Soluble
46 P D -1.2204 Soluble
47 G D -1.7044 Soluble
48 K D -2.4166 Soluble
49 G D -1.7524 Soluble
50 L D 0.0000 Buried
51 E D -1.2772 Soluble
52 W D -0.2647 Soluble
53 V D 0.0000 Buried
54 A D 0.0000 Buried
55 Y D 0.0000 Buried
56 I D 0.0000 Buried
57 K D -1.2530 Soluble
58 Q D -1.5261 Soluble
59 D D -2.6300 Soluble
62 G D -2.2467 Soluble
63 N D -2.4064 Soluble
64 E D -1.9526 Soluble
65 K D -1.1951 Soluble
66 Y D -0.4805 Soluble
67 Y D -0.8083 Soluble
68 V D 0.0000 Buried
69 D D -2.4036 Soluble
70 S D -1.7856 Soluble
71 V D 0.0000 Buried
72 K D -2.5629 Soluble
74 G D -1.7956 Soluble
75 R D -1.4080 Soluble
76 F D 0.0000 Buried
77 T D -0.8714 Soluble
78 I D 0.0000 Buried
79 S D -0.3893 Soluble
80 R D -1.1402 Soluble
81 D D -1.5448 Soluble
82 N D -1.7352 Soluble
83 A D -1.2966 Soluble
84 K D -2.2090 Soluble
85 N D -1.6571 Soluble
86 S D -0.9899 Soluble
87 L D 0.0000 Buried
88 Y D -0.5356 Soluble
89 L D 0.0000 Buried
90 Q D -1.2521 Soluble
91 M D 0.0000 Buried
92 N D -1.6122 Soluble
93 S D -1.5227 Soluble
94 L D 0.0000 Buried
95 R D -2.8775 Soluble
96 A D -1.9945 Soluble
97 E D -2.4314 Soluble
98 D D 0.0000 Buried
99 T D -0.7589 Soluble
100 A D 0.0000 Buried
101 V D 0.0281 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.1623 Aggregation-prone
107 E D 0.0000 Buried
108 G D 0.0000 Buried
109 I D 1.8056 Aggregation-prone
110 L D 2.3900 Aggregation-prone
111 W D 2.5020 Aggregation-prone
112A F D 2.5953 Aggregation-prone
112 G D 0.0000 Buried
113 D D 0.8539 Aggregation-prone
114 L D 0.8284 Aggregation-prone
115 P D 0.3082 Aggregation-prone
116 T D 0.0000 Buried
117 F D 0.3661 Aggregation-prone
118 W D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.4515 Soluble
121 G D -0.7099 Soluble
122 T D -0.2223 Soluble
123 L D 0.1161 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.0000 Buried
126 V D 0.0000 Buried
127 S D -1.0030 Soluble
128 S D -0.7036 Soluble
129 A D -0.4976 Soluble
130 S D -0.5924 Soluble
131 T D -0.6212 Soluble
132 K D -1.2408 Soluble
133 G D -1.4292 Soluble
134 P D -0.6730 Soluble
135 S D -0.2858 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.0440 Soluble
139 L D 0.0000 Buried
140 A D -1.1850 Soluble
141 P D 0.0000 Buried
142 S D -1.1676 Soluble
143 S D -0.9148 Soluble
144 K D -0.9708 Soluble
145 S D 0.0000 Buried
146 T D -0.6700 Soluble
147 S D -0.7794 Soluble
148 G D -0.8613 Soluble
149 G D -0.9289 Soluble
150 T D -0.6221 Soluble
151 A D 0.0000 Buried
152 A D 0.0000 Buried
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D 0.0000 Buried
159 D D -0.4210 Soluble
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -0.3383 Soluble
164 P D -0.6665 Soluble
165 V D -0.7077 Soluble
166 T D -0.6480 Soluble
167 V D -0.2134 Soluble
168 S D -0.3491 Soluble
169 W D 0.0000 Buried
170 N D -0.7172 Soluble
171 S D -0.6063 Soluble
172 G D -0.3486 Soluble
173 A D -0.1714 Soluble
174 L D 0.0096 Aggregation-prone
175 T D -0.1799 Soluble
176 S D -0.2767 Soluble
177 G D -0.4190 Soluble
178 V D 0.0661 Aggregation-prone
179 H D -0.2670 Soluble
180 T D -0.0515 Soluble
181 F D 0.0000 Buried
182 P D -0.3104 Soluble
183 A D 0.3879 Aggregation-prone
184 V D 0.6407 Aggregation-prone
185 L D 1.3982 Aggregation-prone
186 Q D 0.2413 Aggregation-prone
187 S D -0.0374 Soluble
188 S D -0.1619 Soluble
189 G D 0.1474 Aggregation-prone
190 L D 0.2093 Aggregation-prone
191 Y D 0.4944 Aggregation-prone
192 S D 0.3898 Aggregation-prone
193 L D 0.2329 Aggregation-prone
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 V D 0.0000 Buried
198 T D -0.1995 Soluble
199 V D 0.0000 Buried
200 P D -0.5577 Soluble
201 S D -0.6224 Soluble
202 S D -0.6336 Soluble
203 S D 0.0000 Buried
204 L D -0.9172 Soluble
205 G D -0.9841 Soluble
206 T D -0.7907 Soluble
207 Q D -1.3179 Soluble
208 T D -1.2148 Soluble
209 Y D 0.0000 Buried
210 I D -1.1708 Soluble
211 C D 0.0000 Buried
212 N D -1.5493 Soluble
213 V D 0.0000 Buried
214 N D -2.2623 Soluble
215 H D 0.0000 Buried
216 K D -2.8213 Soluble
217 P D -1.5958 Soluble
218 S D -1.8863 Soluble
219 N D -2.6713 Soluble
220 T D -2.1821 Soluble
221 K D -2.8481 Soluble
222 V D -1.6160 Soluble
223 D D -2.5292 Soluble
224 K D -1.8898 Soluble
225 K D -2.4075 Soluble
226 V D 0.0000 Buried
227 E D -2.8147 Soluble
228 P D -2.0762 Soluble
229 K D -2.2801 Soluble
230 S D -1.3495 Soluble
1 D F -1.9732 Soluble
2 I F -1.5605 Soluble
3 Q F -2.0562 Soluble
4 M F 0.0000 Buried
5 T F -1.4872 Soluble
6 Q F 0.0000 Buried
7 S F -0.7571 Soluble
8 P F -0.6287 Soluble
9 S F -0.8959 Soluble
10 S F -0.7740 Soluble
11 L F -0.4919 Soluble
12 S F -0.7303 Soluble
13 A F -1.0109 Soluble
14 S F -0.9960 Soluble
15 V F -0.4344 Soluble
16 G F -0.9172 Soluble
17 D F -1.9855 Soluble
18 R F -2.3860 Soluble
19 V F 0.0000 Buried
20 T F -0.5281 Soluble
21 I F 0.0000 Buried
22 T F -0.9043 Soluble
23 C F 0.0000 Buried
24 R F -2.9261 Soluble
25 A F 0.0000 Buried
26 S F -1.9790 Soluble
27 Q F -2.1012 Soluble
28 G F -1.6885 Soluble
29 I F 0.0000 Buried
36 S F -0.8143 Soluble
37 N F -0.9166 Soluble
38 W F 0.0031 Aggregation-prone
39 L F 0.0000 Buried
40 A F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F -1.5742 Soluble
45 K F -2.0540 Soluble
46 P F -2.0599 Soluble
47 E F -2.8853 Soluble
48 K F -3.1922 Soluble
49 A F -2.0395 Soluble
50 P F 0.0000 Buried
51 K F -1.8255 Soluble
52 S F -0.7307 Soluble
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 Y F 0.3046 Aggregation-prone
56 A F -0.0370 Soluble
57 A F 0.0000 Buried
65 S F -0.2266 Soluble
66 S F -0.0132 Soluble
67 L F 0.1230 Aggregation-prone
68 Q F -0.5228 Soluble
69 S F -0.5175 Soluble
70 G F -0.5800 Soluble
71 V F -0.4320 Soluble
72 P F -0.4020 Soluble
74 S F -0.4267 Soluble
75 R F -0.8149 Soluble
76 F F 0.0000 Buried
77 S F -0.2772 Soluble
78 G F -0.1856 Soluble
79 S F -0.6574 Soluble
80 G F -1.2257 Soluble
83 S F -1.1178 Soluble
84 G F -1.5256 Soluble
85 T F -2.0933 Soluble
86 D F -2.5879 Soluble
87 F F 0.0000 Buried
88 T F -0.7847 Soluble
89 L F 0.0000 Buried
90 T F -0.5951 Soluble
91 I F 0.0000 Buried
92 S F -1.3312 Soluble
93 S F -1.1873 Soluble
94 L F 0.0000 Buried
95 Q F -1.2108 Soluble
96 P F -1.8851 Soluble
97 E F -2.4378 Soluble
98 D F 0.0000 Buried
99 F F -1.1247 Soluble
100 A F 0.0000 Buried
101 T F -0.8112 Soluble
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 Q F 0.0000 Buried
107 Y F 0.0000 Buried
108 D F 0.3376 Aggregation-prone
109 S F 0.5795 Aggregation-prone
114 Y F 0.0000 Buried
115 P F 0.0000 Buried
116 R F 0.0000 Buried
117 T F 0.0000 Buried
118 F F 0.0000 Buried
119 G F 0.0000 Buried
120 Q F -1.7886 Soluble
121 G F 0.0000 Buried
122 T F 0.0000 Buried
123 K F -1.3697 Soluble
124 V F 0.0000 Buried
125 E F -1.1260 Soluble
126 I F 0.0000 Buried
127 K F -1.1389 Soluble
128 R F -0.7917 Soluble
129 T F -0.0021 Soluble
130 V F 0.8418 Aggregation-prone
131 A F 0.1867 Aggregation-prone
132 A F -0.0208 Soluble
133 P F 0.0000 Buried
134 S F -0.1408 Soluble
135 V F -0.1229 Soluble
136 F F 0.0000 Buried
137 I F 0.0000 Buried
138 F F 0.0000 Buried
139 P F -0.6574 Soluble
140 P F 0.0000 Buried
141 S F -1.5624 Soluble
142 D F -2.8734 Soluble
143 E F -2.8281 Soluble
144 Q F 0.0000 Buried
145 L F -2.2640 Soluble
146 K F -2.8433 Soluble
147 S F -1.7986 Soluble
148 G F -1.4579 Soluble
149 T F -1.1644 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -0.8580 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F 0.0000 Buried
162 R F -3.0566 Soluble
163 E F -3.1827 Soluble
164 A F -2.2269 Soluble
165 K F -2.1933 Soluble
166 V F -1.0419 Soluble
167 Q F -0.7104 Soluble
168 W F 0.0000 Buried
169 K F -0.5726 Soluble
170 V F 0.0000 Buried
171 D F -1.5972 Soluble
172 N F -1.2335 Soluble
173 A F -0.1748 Soluble
174 L F 0.7821 Aggregation-prone
175 Q F -0.1465 Soluble
176 S F -0.4942 Soluble
177 G F -0.9509 Soluble
178 N F -0.8542 Soluble
179 S F -1.2780 Soluble
180 Q F -1.7623 Soluble
181 E F -1.9931 Soluble
182 S F -0.8090 Soluble
183 V F -0.6286 Soluble
184 T F 0.0000 Buried
185 E F -1.6364 Soluble
186 Q F 0.0000 Buried
187 D F -2.0586 Soluble
188 S F -2.3072 Soluble
189 K F -2.7421 Soluble
190 D F -2.1140 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.8008 Soluble
199 L F 0.0000 Buried
200 T F -0.4537 Soluble
201 L F -0.6100 Soluble
202 S F -1.1526 Soluble
203 K F -2.1175 Soluble
204 A F -1.9202 Soluble
205 D F -2.4448 Soluble
206 Y F 0.0000 Buried
207 E F -3.8660 Soluble
208 K F -3.6241 Soluble
209 H F -3.2056 Soluble
210 K F -3.4596 Soluble
211 V F -1.7429 Soluble
212 Y F 0.0000 Buried
213 A F 0.0000 Buried
214 C F 0.0000 Buried
215 E F -0.8910 Soluble
216 V F 0.0000 Buried
217 T F -1.1013 Soluble
218 H F 0.0000 Buried
219 Q F -1.6758 Soluble
220 G F -0.3122 Soluble
221 L F -0.1828 Soluble
222 S F -0.4429 Soluble
223 S F -0.4188 Soluble
224 P F -0.5245 Soluble
225 V F 0.0322 Aggregation-prone
226 T F -0.3821 Soluble
227 K F -0.5675 Soluble
228 S F -0.7735 Soluble
229 F F 0.0000 Buried
230 N F -2.2727 Soluble
231 R F -2.4483 Soluble
232 G F -2.9113 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7241 5.9196 CSV PDB
4.5 -0.7671 5.885 CSV PDB
5.0 -0.8172 5.8465 CSV PDB
5.5 -0.865 5.8181 CSV PDB
6.0 -0.8989 5.8016 CSV PDB
6.5 -0.9094 5.7888 CSV PDB
7.0 -0.8959 5.7794 CSV PDB
7.5 -0.8662 5.773 CSV PDB
8.0 -0.8273 5.7704 CSV PDB
8.5 -0.7815 5.772 CSV PDB
9.0 -0.7294 5.7772 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH