Ficerafusp / 5gz0

A4DAB117_5gz0_D_C | Source PDB: 5gz0 | Chains: D, C | Done
98.23
Yes
10 Å
Yes
Avg A4D Score
-0.7156
Max Score
1.8942
Min Score
-3.7557
Total Score
-310.59
Score CDR
-0.2865
Score non-CDR
-0.7665

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPGGSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSDIQMTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGA input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7557
Maximum Score 1.8942
Average Score -0.7156
Total Score -310.5876

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.4039 Soluble
2 V D -0.7564 Soluble
3 Q D -1.3464 Soluble
4 L D 0.0000 Buried
5 K D -1.9036 Soluble
6 Q D 0.0000 Buried
7 S D -0.8208 Soluble
8 G D -1.1935 Soluble
9 P D -0.5310 Soluble
11 G D -0.4069 Soluble
12 L D -0.0682 Soluble
13 V D -0.7073 Soluble
14 Q D -1.5369 Soluble
15 P D -1.5317 Soluble
16 G D -1.5449 Soluble
17 G D -1.3285 Soluble
18 S D -1.6338 Soluble
19 L D 0.0000 Buried
20 S D -0.8752 Soluble
21 I D 0.0000 Buried
22 T D -0.6239 Soluble
23 C D 0.0000 Buried
24 T D -1.0472 Soluble
25 V D 0.0000 Buried
26 S D -1.0617 Soluble
27 G D -0.9132 Soluble
28 F D -0.5038 Soluble
29 S D -0.9453 Soluble
30 L D 0.0000 Buried
35 T D -1.0195 Soluble
36 N D -0.9102 Soluble
37 Y D 0.4046 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D -0.4265 Soluble
44 Q D -0.6579 Soluble
45 S D -0.9966 Soluble
46 P D -0.9476 Soluble
47 G D -1.4414 Soluble
48 K D -2.1253 Soluble
49 G D 0.0000 Buried
50 L D 0.0000 Buried
51 E D -0.8981 Soluble
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5559 Soluble
58 S D -0.7516 Soluble
59 G D -1.2854 Soluble
63 G D -1.5958 Soluble
64 N D -1.8455 Soluble
65 T D -1.2757 Soluble
66 D D -0.9539 Soluble
67 Y D -0.3001 Soluble
68 N D 0.0000 Buried
69 T D -0.4656 Soluble
70 P D -0.3550 Soluble
71 F D 0.0000 Buried
72 T D -0.4217 Soluble
74 S D -0.6574 Soluble
75 R D -1.2505 Soluble
76 L D 0.0000 Buried
77 S D -1.1397 Soluble
78 I D 0.0000 Buried
79 N D -1.9226 Soluble
80 K D -1.7266 Soluble
81 D D -2.0563 Soluble
82 N D -2.6435 Soluble
83 S D -1.8851 Soluble
84 K D -2.4976 Soluble
85 S D -1.7375 Soluble
86 Q D -1.6675 Soluble
87 V D 0.0000 Buried
88 F D -0.6158 Soluble
89 F D 0.0000 Buried
90 K D -1.9235 Soluble
91 M D 0.0000 Buried
92 N D -2.1987 Soluble
93 S D -1.5865 Soluble
94 L D 0.0000 Buried
95 Q D -2.1733 Soluble
96 S D -1.5951 Soluble
97 N D -1.7284 Soluble
98 D D 0.0000 Buried
99 T D -0.5538 Soluble
100 A D 0.0000 Buried
101 I D 0.4294 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.1529 Aggregation-prone
109 T D 1.1768 Aggregation-prone
110 Y D 1.8547 Aggregation-prone
111 Y D 1.8942 Aggregation-prone
112 D D 1.0324 Aggregation-prone
113 Y D 0.5375 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.3328 Aggregation-prone
117 Y D 0.3122 Aggregation-prone
118 W D -0.3813 Soluble
119 G D 0.0000 Buried
120 Q D -1.7092 Soluble
121 G D -0.8595 Soluble
122 T D 0.0000 Buried
123 L D 0.2726 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.3112 Soluble
126 V D 0.0000 Buried
127 S D -0.6700 Soluble
128 A D -0.4316 Soluble
129 A D -0.3387 Soluble
130 S D -0.5021 Soluble
131 T D -0.5856 Soluble
132 K D -1.1988 Soluble
133 G D -1.4008 Soluble
134 P D -0.6209 Soluble
135 S D -0.2818 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.1788 Soluble
139 L D 0.0000 Buried
140 A D -1.2587 Soluble
141 P D 0.0000 Buried
142 S D -1.4104 Soluble
143 S D -1.4191 Soluble
144 K D -1.8927 Soluble
145 S D -1.1583 Soluble
146 T D -0.9868 Soluble
147 S D -0.8769 Soluble
148 G D -0.8152 Soluble
149 G D -0.8950 Soluble
150 T D -0.5960 Soluble
151 A D 0.0000 Buried
152 A D 0.0000 Buried
153 L D 0.0000 Buried
154 G D 0.0000 Buried
155 C D 0.0000 Buried
156 L D 0.0000 Buried
157 V D 0.0000 Buried
158 K D 0.0000 Buried
159 D D -0.5270 Soluble
160 Y D 0.0000 Buried
161 F D 0.0000 Buried
162 P D 0.0000 Buried
163 E D -0.3681 Soluble
164 P D -0.5906 Soluble
165 V D -0.6185 Soluble
166 T D -0.5296 Soluble
167 V D -0.3122 Soluble
168 S D -0.3558 Soluble
169 W D 0.0000 Buried
170 N D -0.7013 Soluble
171 S D -0.6224 Soluble
172 G D -0.5774 Soluble
173 A D -0.2466 Soluble
174 L D 0.0150 Aggregation-prone
175 T D -0.1636 Soluble
176 S D -0.1568 Soluble
177 G D -0.1619 Soluble
178 V D 0.2132 Aggregation-prone
179 H D -0.2866 Soluble
180 T D 0.0793 Aggregation-prone
181 F D 0.0000 Buried
182 P D -0.2810 Soluble
183 A D 0.2267 Aggregation-prone
184 V D 0.4327 Aggregation-prone
185 L D 0.9145 Aggregation-prone
186 Q D 0.1303 Aggregation-prone
187 S D -0.1328 Soluble
188 S D -0.2920 Soluble
189 G D -0.0676 Soluble
190 L D 0.0343 Aggregation-prone
191 Y D 0.3631 Aggregation-prone
192 S D 0.0000 Buried
193 L D 0.0000 Buried
194 S D 0.0000 Buried
195 S D 0.0000 Buried
196 V D 0.0000 Buried
197 V D 0.0000 Buried
198 T D -0.1206 Soluble
199 V D 0.0000 Buried
200 P D -0.6185 Soluble
201 S D -0.5930 Soluble
202 S D -0.5910 Soluble
203 S D -0.6082 Soluble
204 L D -0.7540 Soluble
205 G D -0.9247 Soluble
206 T D -0.6811 Soluble
207 Q D -1.1830 Soluble
208 T D -1.1040 Soluble
209 Y D 0.0000 Buried
210 I D -1.0310 Soluble
211 C D 0.0000 Buried
212 N D -1.3092 Soluble
213 V D 0.0000 Buried
214 N D -1.9202 Soluble
215 H D 0.0000 Buried
216 K D -2.8437 Soluble
217 P D -1.6448 Soluble
218 S D -1.8079 Soluble
219 N D -2.6033 Soluble
220 T D -2.0711 Soluble
221 K D -2.6066 Soluble
222 V D -1.3937 Soluble
223 D D -2.1425 Soluble
224 K D -1.9783 Soluble
225 R D -2.5587 Soluble
226 V D 0.0000 Buried
227 E D -2.9127 Soluble
228 P D -2.2231 Soluble
229 K D -2.3913 Soluble
230 S D -1.3171 Soluble
1 D C -1.9087 Soluble
2 I C -1.5318 Soluble
3 Q C -1.9119 Soluble
4 M C 0.0000 Buried
5 T C -1.0378 Soluble
6 Q C -0.2944 Soluble
7 S C 0.0744 Aggregation-prone
8 P C 0.8081 Aggregation-prone
9 V C 1.8724 Aggregation-prone
10 I C 1.4326 Aggregation-prone
11 L C 0.6307 Aggregation-prone
12 S C -0.2067 Soluble
13 V C 0.0000 Buried
14 S C -1.8326 Soluble
15 P C -2.0273 Soluble
16 G C -2.3722 Soluble
17 E C -3.2330 Soluble
18 R C -3.3484 Soluble
19 V C 0.0000 Buried
20 S C -0.8841 Soluble
21 F C 0.0000 Buried
22 S C -0.9127 Soluble
23 C C 0.0000 Buried
24 R C -2.8686 Soluble
25 A C 0.0000 Buried
26 S C -1.7568 Soluble
27 Q C -1.5647 Soluble
28 S C -1.3934 Soluble
29 I C 0.0000 Buried
36 G C -0.9324 Soluble
37 T C -0.7453 Soluble
38 N C -0.7955 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.1924 Soluble
45 R C -1.9215 Soluble
46 T C -1.6726 Soluble
47 N C -1.9582 Soluble
48 G C -1.4561 Soluble
49 S C -0.9540 Soluble
50 P C 0.0000 Buried
51 R C -0.6651 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.7161 Soluble
56 Y C -0.5460 Soluble
57 A C 0.0000 Buried
65 S C -1.0945 Soluble
66 E C -1.6304 Soluble
67 S C -1.0172 Soluble
68 I C -0.4188 Soluble
69 S C -0.3418 Soluble
70 G C -0.4741 Soluble
71 I C -0.2695 Soluble
72 P C -0.4705 Soluble
74 S C -0.6738 Soluble
75 R C -1.3826 Soluble
76 F C 0.0000 Buried
77 S C -0.9420 Soluble
78 G C -0.6712 Soluble
79 S C -0.7906 Soluble
80 G C -1.0543 Soluble
83 S C -1.0835 Soluble
84 G C -1.2605 Soluble
85 T C -1.8111 Soluble
86 D C -2.4221 Soluble
87 F C 0.0000 Buried
88 T C -0.8248 Soluble
89 L C 0.0000 Buried
90 S C -0.9785 Soluble
91 I C 0.0000 Buried
92 N C -2.9223 Soluble
93 S C -2.7122 Soluble
94 V C 0.0000 Buried
95 E C -2.7658 Soluble
96 S C -2.3615 Soluble
97 E C -2.3108 Soluble
98 D C 0.0000 Buried
99 I C 0.0000 Buried
100 A C -1.4137 Soluble
101 D C -0.7130 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.6109 Soluble
108 N C -1.1335 Soluble
109 N C -0.9299 Soluble
114 W C 0.0848 Aggregation-prone
115 P C 0.0000 Buried
116 T C 0.0000 Buried
117 T C -0.6156 Soluble
118 F C -0.3555 Soluble
119 G C 0.0000 Buried
120 A C -0.6223 Soluble
121 G C 0.0000 Buried
122 T C 0.0000 Buried
123 K C -0.7992 Soluble
124 L C 0.0000 Buried
125 E C -0.8732 Soluble
126 L C 0.0000 Buried
127 K C -1.4469 Soluble
128 R C -0.9092 Soluble
129 T C 0.0382 Aggregation-prone
130 V C 0.6718 Aggregation-prone
131 A C 0.3061 Aggregation-prone
132 A C -0.0511 Soluble
133 P C 0.0000 Buried
134 S C -0.1530 Soluble
135 V C 0.0000 Buried
136 F C 0.0000 Buried
137 I C 0.0000 Buried
138 F C 0.0000 Buried
139 P C -0.5402 Soluble
140 P C -0.8052 Soluble
141 S C -1.8160 Soluble
142 D C -2.9602 Soluble
143 E C -2.9174 Soluble
144 Q C 0.0000 Buried
145 L C -2.0344 Soluble
146 K C -2.8189 Soluble
147 S C -1.7332 Soluble
148 G C -1.3376 Soluble
149 T C -1.2309 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C -0.3638 Soluble
158 N C -0.7696 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.4573 Soluble
162 R C -2.5508 Soluble
163 E C -3.0390 Soluble
164 A C -2.3432 Soluble
165 K C -2.5405 Soluble
166 V C 0.0000 Buried
167 Q C -0.9671 Soluble
168 W C 0.0000 Buried
169 K C -0.9104 Soluble
170 V C 0.0000 Buried
171 D C -2.0005 Soluble
172 N C -1.7148 Soluble
173 A C -0.6182 Soluble
174 L C -0.1567 Soluble
175 Q C -0.6141 Soluble
176 S C -0.6914 Soluble
177 G C -0.9844 Soluble
178 N C -1.0773 Soluble
179 S C -1.3277 Soluble
180 Q C -1.4842 Soluble
181 E C -1.9077 Soluble
182 S C -0.8985 Soluble
183 V C -0.5332 Soluble
184 T C -1.0093 Soluble
185 E C -2.3341 Soluble
186 Q C 0.0000 Buried
187 D C -2.1713 Soluble
188 S C -2.4467 Soluble
189 K C -2.6932 Soluble
190 D C -1.9494 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.7257 Soluble
199 L C 0.0000 Buried
200 T C -0.4583 Soluble
201 L C -0.7923 Soluble
202 S C -1.1979 Soluble
203 K C -2.0731 Soluble
204 A C -2.0515 Soluble
205 D C -3.1053 Soluble
206 Y C 0.0000 Buried
207 E C -3.5426 Soluble
208 K C -3.7557 Soluble
209 H C -3.2077 Soluble
210 K C -2.6865 Soluble
211 V C -1.2356 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9349 Soluble
216 V C 0.0000 Buried
217 T C -1.2634 Soluble
218 H C 0.0000 Buried
219 Q C -1.6929 Soluble
220 G C -0.4021 Soluble
221 L C -0.2214 Soluble
222 S C -0.4616 Soluble
223 S C -0.4249 Soluble
224 P C -0.5877 Soluble
225 V C -0.0029 Soluble
226 T C -0.4318 Soluble
227 K C -0.6908 Soluble
228 S C -0.6269 Soluble
229 F C -0.7667 Soluble
230 N C -1.4550 Soluble
231 R C -1.8264 Soluble
232 G C -1.1537 Soluble
233 A C -0.6632 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7248 2.3819 CSV PDB
4.5 -0.7616 2.302 CSV PDB
5.0 -0.8053 2.2158 CSV PDB
5.5 -0.847 2.1422 CSV PDB
6.0 -0.876 2.1296 CSV PDB
6.5 -0.8837 2.1169 CSV PDB
7.0 -0.8696 2.1043 CSV PDB
7.5 -0.8411 2.0917 CSV PDB
8.0 -0.8048 2.1451 CSV PDB
8.5 -0.7629 2.2562 CSV PDB
9.0 -0.7157 2.3688 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH