Vofatamab / 3grw

A4DAB297_3grw_H_L | Source PDB: 3grw | Chains: H, L | Done
96.15
Yes
10 Å
Yes
Avg A4D Score
-0.6385
Max Score
2.2048
Min Score
-3.5454
Total Score
-283.5
Score CDR
-0.0847
Score non-CDR
-0.7152

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFTSTGISWVRQAPGKGLEWVGRIYPTNGSTNYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARTYGIYDLYVDYTEYVMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5454
Maximum Score 2.2048
Average Score -0.6385
Total Score -283.4969

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0772 Soluble
2 V H -1.1581 Soluble
3 Q H -1.5654 Soluble
4 L H 0.0000 Buried
5 V H 0.3846 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4437 Soluble
8 G H -0.7461 Soluble
9 G H -0.3933 Soluble
11 G H -0.0779 Soluble
12 L H -0.0428 Soluble
13 V H 0.0000 Buried
14 Q H -1.6738 Soluble
15 P H -1.8005 Soluble
16 G H -1.4762 Soluble
17 G H -1.1308 Soluble
18 S H -1.1168 Soluble
19 L H -0.8629 Soluble
20 R H -1.5042 Soluble
21 L H 0.0000 Buried
22 S H -0.3495 Soluble
23 C H 0.0000 Buried
24 A H -0.3132 Soluble
25 A H -0.3280 Soluble
26 S H -0.6357 Soluble
27 G H -0.0646 Soluble
28 F H 1.6078 Aggregation-prone
29 T H 0.6262 Aggregation-prone
30 F H 0.0000 Buried
35 T H -0.3719 Soluble
36 S H -0.2356 Soluble
37 T H -0.3104 Soluble
38 G H 0.0000 Buried
39 I H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7984 Soluble
45 A H -1.1765 Soluble
46 P H -0.9551 Soluble
47 G H -1.5269 Soluble
48 K H -2.3786 Soluble
49 G H -1.6349 Soluble
50 L H 0.0000 Buried
51 E H -1.1101 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 G H 0.0000 Buried
55 R H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.4598 Soluble
58 P H -0.6311 Soluble
59 T H 0.0000 Buried
62 N H -1.6271 Soluble
63 G H -1.0532 Soluble
64 S H -0.7825 Soluble
65 T H -0.6672 Soluble
66 N H -1.1652 Soluble
67 Y H -1.1927 Soluble
68 A H 0.0000 Buried
69 D H -2.7262 Soluble
70 S H -1.4882 Soluble
71 V H 0.0000 Buried
72 K H -2.6123 Soluble
74 G H -1.6242 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.8034 Soluble
78 I H 0.0000 Buried
79 S H -0.5005 Soluble
80 A H -0.9748 Soluble
81 D H -1.5185 Soluble
82 T H -1.3981 Soluble
83 S H -1.3422 Soluble
84 K H -2.1725 Soluble
85 N H -1.2053 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.4357 Soluble
89 L H 0.0000 Buried
90 Q H -0.9144 Soluble
91 M H 0.0000 Buried
92 N H -1.2550 Soluble
93 S H -1.2213 Soluble
94 L H 0.0000 Buried
95 R H -2.4376 Soluble
96 A H -1.8066 Soluble
97 E H -2.2601 Soluble
98 D H 0.0000 Buried
99 T H -0.7472 Soluble
100 A H 0.0000 Buried
101 V H 0.2987 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.1811 Soluble
107 T H 0.0000 Buried
108 Y H 0.4543 Aggregation-prone
109 G H 0.5755 Aggregation-prone
110 I H 0.8159 Aggregation-prone
111 Y H 1.1703 Aggregation-prone
111A D H 0.1212 Aggregation-prone
111B L H 1.4936 Aggregation-prone
111C Y H 1.5909 Aggregation-prone
112D V H 0.5213 Aggregation-prone
112C D H -0.9448 Soluble
112B Y H -0.0491 Soluble
112A T H 0.3218 Aggregation-prone
112 E H 0.0000 Buried
113 Y H 0.6777 Aggregation-prone
114 V H 0.0000 Buried
115 M H 0.0000 Buried
116 D H 0.0000 Buried
117 Y H -0.6327 Soluble
118 W H -0.6157 Soluble
119 G H 0.0000 Buried
120 Q H -1.5387 Soluble
121 G H -0.6182 Soluble
122 T H -0.0421 Soluble
123 L H 0.2863 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.0447 Soluble
128 S H -0.7299 Soluble
129 A H -0.5034 Soluble
130 S H -0.6030 Soluble
131 T H -0.5950 Soluble
132 K H -1.2100 Soluble
133 G H -1.3526 Soluble
134 P H 0.0000 Buried
135 S H -0.1576 Soluble
136 V H 0.0000 Buried
137 F H -0.5772 Soluble
138 P H -0.9415 Soluble
139 L H 0.0000 Buried
140 A H -1.3144 Soluble
141 P H 0.0000 Buried
142 S H -1.2648 Soluble
143 S H -0.8604 Soluble
144 K H -1.5431 Soluble
145 S H 0.0000 Buried
146 T H -0.8410 Soluble
147 S H -0.8398 Soluble
148 G H -0.8509 Soluble
149 G H -0.9085 Soluble
150 T H -0.6108 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.3294 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.6115 Soluble
164 P H -0.7609 Soluble
165 V H -0.6826 Soluble
166 T H -0.5995 Soluble
167 V H -0.4073 Soluble
168 S H -0.4045 Soluble
169 W H 0.0000 Buried
170 N H -0.6281 Soluble
171 S H -0.6420 Soluble
172 G H -0.5743 Soluble
173 A H -0.2236 Soluble
174 L H 0.0531 Aggregation-prone
175 T H -0.1392 Soluble
176 S H -0.3936 Soluble
177 G H -0.4508 Soluble
178 V H 0.1974 Aggregation-prone
179 H H 0.0000 Buried
180 T H -0.1295 Soluble
181 F H 0.0000 Buried
182 P H -0.5166 Soluble
183 A H 0.1169 Aggregation-prone
184 V H 0.3971 Aggregation-prone
185 L H 0.8977 Aggregation-prone
186 Q H 0.1456 Aggregation-prone
187 S H -0.3067 Soluble
188 S H -0.2175 Soluble
189 G H 0.0075 Aggregation-prone
190 L H 0.1061 Aggregation-prone
191 Y H 0.2944 Aggregation-prone
192 S H 0.2260 Aggregation-prone
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1068 Soluble
199 V H 0.0000 Buried
200 P H -0.6262 Soluble
201 S H -0.6419 Soluble
202 S H -0.5526 Soluble
203 S H -0.5741 Soluble
204 L H -0.7085 Soluble
205 G H -0.9521 Soluble
206 T H -0.6658 Soluble
207 Q H -1.2055 Soluble
208 T H -0.9703 Soluble
209 Y H 0.0000 Buried
210 I H -0.8752 Soluble
211 C H 0.0000 Buried
212 N H -1.5368 Soluble
213 V H 0.0000 Buried
214 N H -1.9568 Soluble
215 H H 0.0000 Buried
216 K H -2.7301 Soluble
217 P H -1.5886 Soluble
218 S H -1.8409 Soluble
219 N H -2.5939 Soluble
220 T H -2.0550 Soluble
221 K H -2.6535 Soluble
222 V H -1.3994 Soluble
223 D H -2.4362 Soluble
224 K H -1.7714 Soluble
225 K H -2.2109 Soluble
226 V H 0.0000 Buried
227 E H -2.7640 Soluble
228 P H -1.9081 Soluble
229 K H -2.2984 Soluble
230 S H -0.8805 Soluble
231 C H 0.0207 Aggregation-prone
1 D L -2.4122 Soluble
2 I L 0.0000 Buried
3 Q L -2.2130 Soluble
4 M L 0.0000 Buried
5 T L -1.4269 Soluble
6 Q L 0.0000 Buried
7 S L -0.7045 Soluble
8 P L -0.6220 Soluble
9 S L -0.8807 Soluble
10 S L -0.8360 Soluble
11 L L -0.5239 Soluble
12 S L -0.8145 Soluble
13 A L 0.0000 Buried
14 S L -0.6425 Soluble
15 V L 0.1391 Aggregation-prone
16 G L -0.7871 Soluble
17 D L -1.6400 Soluble
18 R L -2.2789 Soluble
19 V L 0.0000 Buried
20 T L -0.6246 Soluble
21 I L 0.0000 Buried
22 T L -0.8355 Soluble
23 C L 0.0000 Buried
24 R L -2.8794 Soluble
25 A L 0.0000 Buried
26 S L -2.1160 Soluble
27 Q L -2.4215 Soluble
28 D L -2.5430 Soluble
29 V L 0.0000 Buried
36 S L -0.7731 Soluble
37 T L -0.5022 Soluble
38 A L 0.0000 Buried
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.8137 Soluble
46 P L -1.5832 Soluble
47 G L -1.6784 Soluble
48 K L -2.6332 Soluble
49 A L -1.6601 Soluble
50 P L 0.0000 Buried
51 K L -1.4467 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 1.1342 Aggregation-prone
56 S L 0.7740 Aggregation-prone
57 A L 0.0000 Buried
65 S L 0.8036 Aggregation-prone
66 F L 2.2048 Aggregation-prone
67 L L 1.5042 Aggregation-prone
68 Y L 0.8202 Aggregation-prone
69 S L 0.0760 Aggregation-prone
70 G L -0.3932 Soluble
71 V L -0.1969 Soluble
72 P L -0.2756 Soluble
74 S L -0.3746 Soluble
75 R L -0.7959 Soluble
76 F L 0.0000 Buried
77 S L 0.0548 Aggregation-prone
78 G L 0.1674 Aggregation-prone
79 S L -0.3527 Soluble
80 G L -1.0036 Soluble
83 S L -1.0409 Soluble
84 G L -1.7037 Soluble
85 T L -2.1442 Soluble
86 D L -2.3153 Soluble
87 F L 0.0000 Buried
88 T L -0.7463 Soluble
89 L L 0.0000 Buried
90 T L -0.6228 Soluble
91 I L 0.0000 Buried
92 S L -1.3055 Soluble
93 S L -1.1446 Soluble
94 L L 0.0000 Buried
95 Q L -0.9307 Soluble
96 P L -0.9166 Soluble
97 E L -1.9130 Soluble
98 D L 0.0000 Buried
99 F L -0.6387 Soluble
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 S L 0.0000 Buried
108 Y L 0.0322 Aggregation-prone
109 T L -0.0217 Soluble
114 T L -0.3359 Soluble
115 P L -0.8169 Soluble
116 P L 0.0000 Buried
117 T L -0.7352 Soluble
118 F L -0.4699 Soluble
119 G L 0.0000 Buried
120 Q L -1.6593 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.6719 Soluble
124 V L 0.0000 Buried
125 E L -1.1135 Soluble
126 I L -0.6627 Soluble
127 K L -1.5546 Soluble
128 R L -1.2741 Soluble
129 T L -0.1540 Soluble
130 V L 0.4719 Aggregation-prone
131 A L 0.0626 Aggregation-prone
132 A L -0.0521 Soluble
133 P L 0.0000 Buried
134 S L -0.1078 Soluble
135 V L -0.2452 Soluble
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.6892 Soluble
140 P L 0.0000 Buried
141 S L -1.7500 Soluble
142 D L -3.0742 Soluble
143 E L -2.6184 Soluble
144 Q L 0.0000 Buried
145 L L -2.1905 Soluble
146 K L -2.7276 Soluble
147 S L -1.7180 Soluble
148 G L -1.2278 Soluble
149 T L -0.9962 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7648 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6659 Soluble
162 R L -2.8676 Soluble
163 E L -3.1450 Soluble
164 A L -2.2857 Soluble
165 K L -2.4149 Soluble
166 V L -1.0502 Soluble
167 Q L -0.6029 Soluble
168 W L 0.0000 Buried
169 K L -0.5656 Soluble
170 V L 0.0000 Buried
171 D L -1.9706 Soluble
172 N L -1.5692 Soluble
173 A L -0.2935 Soluble
174 L L 0.6402 Aggregation-prone
175 Q L -0.3268 Soluble
176 S L -0.6593 Soluble
177 G L -1.2642 Soluble
178 N L -1.5811 Soluble
179 S L -1.4079 Soluble
180 Q L -1.4458 Soluble
181 E L -1.5674 Soluble
182 S L -0.7082 Soluble
183 V L -0.8119 Soluble
184 T L -1.1481 Soluble
185 E L -2.2366 Soluble
186 Q L -1.7997 Soluble
187 D L -1.7666 Soluble
188 S L -1.7141 Soluble
189 K L -2.3276 Soluble
190 D L -1.8284 Soluble
191 S L -1.7603 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7032 Soluble
199 L L 0.0000 Buried
200 T L -0.6422 Soluble
201 L L -0.7295 Soluble
202 S L -1.1503 Soluble
203 K L -1.9769 Soluble
204 A L -1.7830 Soluble
205 D L -2.3859 Soluble
206 Y L 0.0000 Buried
207 E L -3.3836 Soluble
208 K L -3.5454 Soluble
209 H L -2.9603 Soluble
210 K L -3.2978 Soluble
211 V L -1.5240 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6537 Soluble
216 V L 0.0000 Buried
217 T L -1.2770 Soluble
218 H L 0.0000 Buried
219 Q L -1.7357 Soluble
220 G L -0.4236 Soluble
221 L L -0.2665 Soluble
222 S L -0.5105 Soluble
223 S L -0.4733 Soluble
224 P L -0.6085 Soluble
225 V L -0.0222 Soluble
226 T L -0.4306 Soluble
227 K L -0.8744 Soluble
228 S L -0.7809 Soluble
229 F L 0.0000 Buried
230 N L -1.8643 Soluble
231 R L -2.4975 Soluble
232 G L -2.1012 Soluble
233 E L -2.0656 Soluble
234 C L -0.4248 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.596 3.9822 CSV PDB
4.5 -0.6406 3.9822 CSV PDB
5.0 -0.6923 3.9822 CSV PDB
5.5 -0.7417 3.9822 CSV PDB
6.0 -0.7777 3.9822 CSV PDB
6.5 -0.7917 3.9822 CSV PDB
7.0 -0.7834 3.9822 CSV PDB
7.5 -0.7602 3.9822 CSV PDB
8.0 -0.7283 3.9822 CSV PDB
8.5 -0.6898 3.9822 CSV PDB
9.0 -0.6437 3.9822 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH