Sabestomig / 8tft

A4DAB233_8tft_I_M | Source PDB: 8tft | Chains: I, M | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6851
Max Score
1.4856
Min Score
-3.2142
Total Score
-298.04
Score CDR
-0.3747
Score non-CDR
-0.7264

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGSYGTYYGNYFEYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSSYVLTQPPSVSVAPGKTARITCGGDNIGGKSVHWYQQKPGQAPVLVIYYDSDRPSGIPERFSGSNSGNTATLTISRVEAGDEADYYCQVLDRRSDHWLFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT input.pdb
Selected Chain(s) I (Heavy), M (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2142
Maximum Score 1.4856
Average Score -0.6851
Total Score -298.0392

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E I -2.0165 Soluble
2 V I -1.0238 Soluble
3 Q I -0.9274 Soluble
4 L I 0.0000 Buried
5 L I 0.6873 Aggregation-prone
6 E I 0.0831 Aggregation-prone
7 S I -0.4575 Soluble
8 G I -0.7657 Soluble
9 G I 0.0000 Buried
11 G I -0.2273 Soluble
12 L I -0.0452 Soluble
13 V I 0.0000 Buried
14 Q I -1.8114 Soluble
15 P I -2.0506 Soluble
16 G I -1.5847 Soluble
17 G I -1.2133 Soluble
18 S I -1.3302 Soluble
19 L I -1.1483 Soluble
20 R I -2.0956 Soluble
21 L I 0.0000 Buried
22 S I -0.4950 Soluble
23 C I 0.0000 Buried
24 A I -0.1291 Soluble
25 A I 0.0000 Buried
26 S I -0.8280 Soluble
27 G I -1.1293 Soluble
28 F I -0.4264 Soluble
29 T I -0.0897 Soluble
30 F I 0.0000 Buried
35 S I -0.6049 Soluble
36 S I 0.1570 Aggregation-prone
37 Y I 0.5121 Aggregation-prone
38 A I 0.0000 Buried
39 M I 0.0000 Buried
40 S I 0.0000 Buried
41 W I 0.0000 Buried
42 V I 0.0000 Buried
43 R I 0.0000 Buried
44 Q I -0.8883 Soluble
45 A I -1.2389 Soluble
46 P I -0.9958 Soluble
47 G I -1.4699 Soluble
48 K I -2.3027 Soluble
49 G I -1.5352 Soluble
50 L I 0.0000 Buried
51 E I -1.1013 Soluble
52 W I 0.0000 Buried
53 V I 0.0000 Buried
54 S I 0.0000 Buried
55 A I 0.0000 Buried
56 I I 0.0000 Buried
57 S I 0.0000 Buried
58 G I -0.0663 Soluble
59 S I -0.5673 Soluble
62 G I -0.8258 Soluble
63 G I -0.5415 Soluble
64 S I -0.1674 Soluble
65 T I -0.1428 Soluble
66 Y I -0.3543 Soluble
67 Y I -0.9545 Soluble
68 A I 0.0000 Buried
69 D I -2.4883 Soluble
70 S I -1.6082 Soluble
71 V I 0.0000 Buried
72 K I -2.6149 Soluble
74 G I -1.7771 Soluble
75 R I -1.3812 Soluble
76 F I 0.0000 Buried
77 T I -0.8651 Soluble
78 I I 0.0000 Buried
79 S I -0.5709 Soluble
80 R I -1.2045 Soluble
81 D I -1.9601 Soluble
82 N I -1.8948 Soluble
83 S I -1.6950 Soluble
84 K I -2.4652 Soluble
85 N I -1.7853 Soluble
86 T I 0.0000 Buried
87 L I 0.0000 Buried
88 Y I -0.7040 Soluble
89 L I 0.0000 Buried
90 Q I -1.3396 Soluble
91 M I 0.0000 Buried
92 N I -1.4854 Soluble
93 S I -1.4026 Soluble
94 L I 0.0000 Buried
95 R I -2.8554 Soluble
96 A I -2.0169 Soluble
97 E I -2.3811 Soluble
98 D I 0.0000 Buried
99 T I -0.8604 Soluble
100 A I 0.0000 Buried
101 V I 0.1815 Aggregation-prone
102 Y I 0.0000 Buried
103 Y I 0.0000 Buried
104 C I 0.0000 Buried
105 A I 0.0000 Buried
106 R I 0.0000 Buried
107 G I 0.0000 Buried
108 S I 0.6655 Aggregation-prone
109 Y I 1.1917 Aggregation-prone
110 G I 0.4985 Aggregation-prone
111 T I 0.8134 Aggregation-prone
111A Y I 1.3619 Aggregation-prone
112A Y I 0.4514 Aggregation-prone
112 G I 0.0000 Buried
113 N I 0.1155 Aggregation-prone
114 Y I 0.0000 Buried
115 F I 0.0000 Buried
116 E I 0.1907 Aggregation-prone
117 Y I 0.5206 Aggregation-prone
118 W I 0.2640 Aggregation-prone
119 G I 0.0000 Buried
120 Q I -1.1914 Soluble
121 G I -0.4699 Soluble
122 T I -0.0365 Soluble
123 L I 0.1757 Aggregation-prone
124 V I 0.0000 Buried
125 T I -0.4928 Soluble
126 V I 0.0000 Buried
127 S I -1.1187 Soluble
128 S I -0.7015 Soluble
129 A I -0.4668 Soluble
130 S I -0.5704 Soluble
131 T I -0.6855 Soluble
132 K I -0.9951 Soluble
133 G I -1.4057 Soluble
134 P I -0.6710 Soluble
135 S I -0.3412 Soluble
136 V I 0.0000 Buried
137 F I 0.0000 Buried
138 P I -1.0333 Soluble
139 L I 0.0000 Buried
140 A I -1.1309 Soluble
141 P I 0.0000 Buried
142 S I -1.0265 Soluble
143 S I -1.3418 Soluble
144 K I -1.9325 Soluble
145 S I -1.2670 Soluble
146 T I -0.9931 Soluble
147 S I -0.8684 Soluble
148 G I -0.8457 Soluble
149 G I -0.9021 Soluble
150 T I -0.7225 Soluble
151 A I 0.0000 Buried
152 A I -0.1156 Soluble
153 L I 0.0000 Buried
154 G I 0.0000 Buried
155 C I 0.0000 Buried
156 L I 0.0000 Buried
157 V I 0.0000 Buried
158 K I -0.3378 Soluble
159 D I -0.4286 Soluble
160 Y I 0.0000 Buried
161 F I -0.1716 Soluble
162 P I 0.0000 Buried
163 E I -0.3748 Soluble
164 P I -0.7264 Soluble
165 V I -0.7346 Soluble
166 T I -0.6657 Soluble
167 V I -0.4139 Soluble
168 S I -0.4673 Soluble
169 W I 0.0000 Buried
170 N I -0.8307 Soluble
171 S I -0.7039 Soluble
172 G I -0.5838 Soluble
173 A I -0.2575 Soluble
174 L I -0.0231 Soluble
175 T I -0.2131 Soluble
176 S I -0.2722 Soluble
177 G I -0.3403 Soluble
178 V I 0.1860 Aggregation-prone
179 H I -0.1039 Soluble
180 T I 0.0258 Aggregation-prone
181 F I 0.0000 Buried
182 P I 0.0076 Aggregation-prone
183 A I 0.2908 Aggregation-prone
184 V I 0.0000 Buried
185 L I 1.2987 Aggregation-prone
186 Q I 0.3191 Aggregation-prone
187 S I -0.1081 Soluble
188 S I -0.1854 Soluble
189 G I 0.0742 Aggregation-prone
190 L I 0.2238 Aggregation-prone
191 Y I 0.4443 Aggregation-prone
192 S I 0.0000 Buried
193 L I 0.0000 Buried
194 S I 0.0000 Buried
195 S I 0.0000 Buried
196 V I 0.1847 Aggregation-prone
197 V I 0.0000 Buried
198 T I -0.2251 Soluble
199 V I 0.0000 Buried
200 P I -0.7010 Soluble
201 S I -0.7295 Soluble
202 S I -0.6013 Soluble
203 S I -0.6426 Soluble
204 L I -0.7747 Soluble
205 G I -0.9421 Soluble
206 T I -0.7161 Soluble
207 Q I -1.1927 Soluble
208 T I -1.1161 Soluble
209 Y I 0.0000 Buried
210 I I -1.3426 Soluble
211 C I 0.0000 Buried
212 N I -1.5674 Soluble
213 V I 0.0000 Buried
214 N I -2.2376 Soluble
215 H I 0.0000 Buried
216 K I -2.8487 Soluble
217 P I -1.5784 Soluble
218 S I -1.8253 Soluble
219 N I -2.5275 Soluble
220 T I -2.1553 Soluble
221 K I -2.8238 Soluble
222 V I -1.6890 Soluble
223 D I -2.5900 Soluble
224 K I -2.1901 Soluble
225 R I -2.6722 Soluble
226 V I 0.0000 Buried
227 E I -2.7049 Soluble
228 P I -1.6124 Soluble
229 K I -1.6688 Soluble
230 S I -1.0130 Soluble
1 S M 0.0482 Aggregation-prone
2 Y M 0.3210 Aggregation-prone
3 V M 1.4856 Aggregation-prone
4 L M 0.0000 Buried
5 T M -0.0301 Soluble
6 Q M 0.0000 Buried
7 P M -0.4491 Soluble
8 P M -0.7319 Soluble
9 S M -0.6824 Soluble
11 V M -0.5653 Soluble
12 S M -0.1777 Soluble
13 V M 0.0000 Buried
14 A M -0.7379 Soluble
15 P M -1.5583 Soluble
16 G M -2.3832 Soluble
17 K M -2.7440 Soluble
18 T M -1.9616 Soluble
19 A M 0.0000 Buried
20 R M -1.8758 Soluble
21 I M 0.0000 Buried
22 T M -0.7224 Soluble
23 C M 0.0000 Buried
24 G M -0.9418 Soluble
25 G M -1.4854 Soluble
26 D M -2.7838 Soluble
27 N M -3.1609 Soluble
28 I M 0.0000 Buried
29 G M -2.0509 Soluble
36 G M -1.7182 Soluble
37 K M -1.1478 Soluble
38 S M -0.3334 Soluble
39 V M 0.0000 Buried
40 H M 0.0000 Buried
41 W M 0.0000 Buried
42 Y M 0.0000 Buried
43 Q M -0.3040 Soluble
44 Q M 0.0000 Buried
45 K M -1.5169 Soluble
46 P M -1.3663 Soluble
47 G M -1.3852 Soluble
48 Q M -1.7785 Soluble
49 A M -0.9605 Soluble
50 P M 0.0000 Buried
51 V M 0.6101 Aggregation-prone
52 L M 0.0000 Buried
53 V M 0.0000 Buried
54 I M 0.0000 Buried
55 Y M -0.4131 Soluble
56 Y M -0.1423 Soluble
57 D M -0.7971 Soluble
65 S M -1.1069 Soluble
66 D M -1.6717 Soluble
67 R M -1.8119 Soluble
68 P M -0.8329 Soluble
69 S M -0.7216 Soluble
70 G M -0.8165 Soluble
71 I M -0.6617 Soluble
72 P M -1.2884 Soluble
74 E M -2.2238 Soluble
75 R M -1.7276 Soluble
76 F M 0.0000 Buried
77 S M -1.3807 Soluble
78 G M -0.8967 Soluble
79 S M -0.6613 Soluble
80 N M -1.0935 Soluble
83 S M -1.4052 Soluble
84 G M -2.1611 Soluble
85 N M -2.8244 Soluble
86 T M -1.5238 Soluble
87 A M 0.0000 Buried
88 T M -0.7536 Soluble
89 L M 0.0000 Buried
90 T M -0.9119 Soluble
91 I M 0.0000 Buried
92 S M -2.4197 Soluble
93 R M -3.1332 Soluble
94 V M 0.0000 Buried
95 E M -2.6266 Soluble
96 A M -1.1464 Soluble
97 G M -1.0056 Soluble
98 D M 0.0000 Buried
99 E M 0.0000 Buried
100 A M 0.0000 Buried
101 D M -1.2037 Soluble
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 Q M 0.0000 Buried
106 V M 0.0000 Buried
107 L M 0.0000 Buried
108 D M 0.0000 Buried
109 R M -2.9882 Soluble
110 R M -2.9617 Soluble
113 S M -2.0299 Soluble
114 D M -1.7895 Soluble
115 H M -1.2431 Soluble
116 W M 0.0000 Buried
117 L M 0.4412 Aggregation-prone
118 F M 0.2770 Aggregation-prone
119 G M 0.0000 Buried
120 G M -0.7252 Soluble
121 G M 0.0000 Buried
122 T M 0.0000 Buried
123 K M -1.2359 Soluble
124 L M 0.0000 Buried
125 T M -0.0454 Soluble
126 V M 0.0000 Buried
127 L M 1.0142 Aggregation-prone
128 G M -0.1449 Soluble
129 Q M 0.0000 Buried
130 P M -1.0558 Soluble
131 K M -2.1466 Soluble
132 A M -1.3407 Soluble
133 A M -0.8654 Soluble
134 P M -0.5394 Soluble
135 S M -0.4325 Soluble
136 V M 0.0000 Buried
137 T M -0.4229 Soluble
138 L M 0.0000 Buried
139 F M 0.0000 Buried
140 P M -0.0536 Soluble
141 P M 0.0000 Buried
142 S M -1.0899 Soluble
143 S M -1.5088 Soluble
144 E M -2.3534 Soluble
145 E M 0.0000 Buried
146 L M -2.1709 Soluble
147 Q M -2.3955 Soluble
148 A M -1.9912 Soluble
149 N M -2.8166 Soluble
150 K M -2.9530 Soluble
151 A M 0.0000 Buried
152 T M 0.0000 Buried
153 L M 0.0000 Buried
154 V M 0.0000 Buried
155 C M 0.0000 Buried
156 L M 0.0000 Buried
157 I M 0.0000 Buried
158 S M -0.7426 Soluble
159 D M -1.4930 Soluble
160 F M 0.0000 Buried
161 Y M -1.1084 Soluble
162 P M -0.9586 Soluble
163 G M -0.4730 Soluble
164 A M -0.1425 Soluble
165 V M -0.0354 Soluble
166 T M -0.0497 Soluble
167 V M 0.1802 Aggregation-prone
168 A M -0.4006 Soluble
169 W M 0.0000 Buried
170 K M -0.9693 Soluble
171 A M 0.0000 Buried
172 D M -1.1977 Soluble
173 S M -0.8112 Soluble
174 S M -0.5102 Soluble
175 P M -0.8206 Soluble
176 V M -0.8306 Soluble
177 K M -1.6303 Soluble
178 A M -0.8198 Soluble
179 G M -0.6539 Soluble
180 V M -0.2861 Soluble
181 E M -0.2246 Soluble
182 T M 0.0828 Aggregation-prone
183 T M 0.0820 Aggregation-prone
184 T M 0.0708 Aggregation-prone
185 P M -0.1831 Soluble
186 S M -0.4904 Soluble
187 K M -1.1082 Soluble
188 Q M -1.2045 Soluble
189 S M -1.0750 Soluble
190 N M -1.5516 Soluble
191 N M -1.2907 Soluble
192 K M -1.5514 Soluble
193 Y M -0.7909 Soluble
194 A M 0.0000 Buried
195 A M 0.0000 Buried
196 S M 0.0000 Buried
197 S M 0.0000 Buried
198 Y M 0.0000 Buried
199 L M 0.0000 Buried
200 S M -0.6598 Soluble
201 L M 0.0000 Buried
202 T M -1.9944 Soluble
203 P M -2.5805 Soluble
204 E M -3.2142 Soluble
205 Q M -2.5126 Soluble
206 W M 0.0000 Buried
207 K M -3.1248 Soluble
208 S M -2.4046 Soluble
209 H M -2.3563 Soluble
210 R M -2.5676 Soluble
211 S M -1.4646 Soluble
212 Y M 0.0000 Buried
213 S M 0.0000 Buried
214 C M 0.0000 Buried
215 Q M -0.8018 Soluble
216 V M 0.0000 Buried
217 T M -0.5982 Soluble
218 H M 0.0000 Buried
219 E M -2.2713 Soluble
220 G M -1.4472 Soluble
221 S M -0.9164 Soluble
222 T M -0.6432 Soluble
223 V M -0.5255 Soluble
224 E M -1.7161 Soluble
225 K M -1.4941 Soluble
226 T M -0.8564 Soluble
227 V M -0.5693 Soluble
228 A M -0.8376 Soluble
229 P M -0.9671 Soluble
230 T M -0.5688 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6976 1.9711 CSV PDB
4.5 -0.7331 1.9711 CSV PDB
5.0 -0.7742 1.9711 CSV PDB
5.5 -0.8123 1.9711 CSV PDB
6.0 -0.8382 1.9711 CSV PDB
6.5 -0.8447 1.9711 CSV PDB
7.0 -0.8315 1.9711 CSV PDB
7.5 -0.8053 1.9711 CSV PDB
8.0 -0.772 1.9711 CSV PDB
8.5 -0.7335 1.9711 CSV PDB
9.0 -0.6903 1.9711 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH