Empasiprubart / 8aci

A4DAB103_8aci_H_L | Source PDB: 8aci | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7635
Max Score
1.1328
Min Score
-3.5025
Total Score
-333.66
Score CDR
-0.6788
Score non-CDR
-0.7742

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGASVKVSCKASGYTFTDYNMDWVRQATGQGLEWIGDINPNYESTGYNQKFKGRATMTVDKSISTAYMELSSLRSEDTAVYYCAREDDHDAFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDNVLTQSPDSLAVSLGERATISCRASKSVRTSGYNYMHWYQQKPGQPPKLLIYLASNLKSGVPDRFSGSGSGTDFTLTISSLQAEDAATYYCQHSRELPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5025
Maximum Score 1.1328
Average Score -0.7635
Total Score -333.6562

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1572 Soluble
2 V H -1.3081 Soluble
3 Q H -1.5862 Soluble
4 L H 0.0000 Buried
5 V H 0.2930 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.5838 Soluble
8 G H -0.5547 Soluble
9 A H -0.3043 Soluble
11 E H -0.6905 Soluble
12 V H -0.1490 Soluble
13 K H -1.1840 Soluble
14 K H -1.8921 Soluble
15 P H -1.9688 Soluble
16 G H -1.4298 Soluble
17 A H -0.9762 Soluble
18 S H -1.2433 Soluble
19 V H 0.0000 Buried
20 K H -2.0930 Soluble
21 V H 0.0000 Buried
22 S H -0.6320 Soluble
23 C H 0.0000 Buried
24 K H -0.9336 Soluble
25 A H 0.0000 Buried
26 S H -0.8880 Soluble
27 G H -1.2184 Soluble
28 Y H -0.7245 Soluble
29 T H -0.5733 Soluble
30 F H 0.0000 Buried
35 T H -1.2498 Soluble
36 D H -2.1227 Soluble
37 Y H -1.3200 Soluble
38 N H -1.1366 Soluble
39 M H 0.0000 Buried
40 D H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.3734 Soluble
44 Q H -0.6191 Soluble
45 A H -0.9108 Soluble
46 T H -0.6593 Soluble
47 G H -1.1621 Soluble
48 Q H -1.7315 Soluble
49 G H -1.2145 Soluble
50 L H 0.0000 Buried
51 E H -0.6208 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 D H -0.5139 Soluble
56 I H 0.0000 Buried
57 N H -1.1327 Soluble
58 P H 0.0000 Buried
59 N H -1.2687 Soluble
62 Y H -0.0379 Soluble
63 E H -1.6527 Soluble
64 S H -0.8516 Soluble
65 T H -0.4521 Soluble
66 G H -0.0049 Soluble
67 Y H -0.4095 Soluble
68 N H -1.3671 Soluble
69 Q H -2.4793 Soluble
70 K H -2.9107 Soluble
71 F H 0.0000 Buried
72 K H -2.9157 Soluble
74 G H -1.9285 Soluble
75 R H -1.6235 Soluble
76 A H 0.0000 Buried
77 T H -0.6529 Soluble
78 M H 0.0000 Buried
79 T H -0.8143 Soluble
80 V H 0.0000 Buried
81 D H -1.3179 Soluble
82 K H -1.5797 Soluble
83 S H -0.4326 Soluble
84 I H 0.2580 Aggregation-prone
85 S H -0.5676 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.6896 Soluble
89 M H 0.0000 Buried
90 E H -1.5139 Soluble
91 L H 0.0000 Buried
92 S H -1.0963 Soluble
93 S H -1.0286 Soluble
94 L H 0.0000 Buried
95 R H -2.7563 Soluble
96 S H -2.2120 Soluble
97 E H -2.4541 Soluble
98 D H 0.0000 Buried
99 T H -0.9284 Soluble
100 A H 0.0000 Buried
101 V H 0.1708 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 E H -0.8914 Soluble
108 D H 0.0000 Buried
109 D H -2.2480 Soluble
110 H H -1.1284 Soluble
113 D H -1.2007 Soluble
114 A H 0.0000 Buried
115 F H 0.0000 Buried
116 A H -0.3712 Soluble
117 Y H -0.0616 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.1611 Soluble
121 G H -0.5895 Soluble
122 T H 0.0000 Buried
123 L H -0.0146 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.2380 Soluble
128 S H -0.9952 Soluble
129 A H -0.5703 Soluble
130 S H -0.6430 Soluble
131 T H -0.6649 Soluble
132 K H -1.1749 Soluble
133 G H -1.3712 Soluble
134 P H 0.0000 Buried
135 S H -0.3111 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1395 Soluble
139 L H 0.0000 Buried
140 A H -1.2815 Soluble
141 P H 0.0000 Buried
142 S H -1.1933 Soluble
143 S H -0.7766 Soluble
144 K H -0.8305 Soluble
145 S H 0.0000 Buried
146 T H -0.6229 Soluble
147 S H -0.6706 Soluble
148 G H -0.8170 Soluble
149 G H -0.8965 Soluble
150 T H -0.6116 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.5117 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4210 Soluble
164 P H -0.7406 Soluble
165 V H -0.7120 Soluble
166 T H -0.6188 Soluble
167 V H -0.3586 Soluble
168 S H -0.3499 Soluble
169 W H 0.0000 Buried
170 N H -0.5904 Soluble
171 S H -0.5919 Soluble
172 G H -0.4270 Soluble
173 A H -0.1993 Soluble
174 L H 0.0722 Aggregation-prone
175 T H -0.1351 Soluble
176 S H -0.3559 Soluble
177 G H -0.4002 Soluble
178 V H 0.2044 Aggregation-prone
179 H H -0.3498 Soluble
180 T H -0.1862 Soluble
181 F H 0.0000 Buried
182 P H -0.4068 Soluble
183 A H 0.1356 Aggregation-prone
184 V H 0.4150 Aggregation-prone
185 L H 1.1328 Aggregation-prone
186 Q H 0.2358 Aggregation-prone
187 S H -0.0597 Soluble
188 S H -0.2101 Soluble
189 G H 0.0155 Aggregation-prone
190 L H 0.0485 Aggregation-prone
191 Y H 0.3449 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1472 Soluble
199 V H 0.0000 Buried
200 P H -0.6348 Soluble
201 S H -0.6045 Soluble
202 S H -0.5910 Soluble
203 S H -0.6057 Soluble
204 L H -0.7798 Soluble
205 G H -0.9374 Soluble
206 T H -0.6761 Soluble
207 Q H -1.1383 Soluble
208 T H -1.0172 Soluble
209 Y H 0.0000 Buried
210 I H -1.0091 Soluble
211 C H 0.0000 Buried
212 N H -1.4380 Soluble
213 V H 0.0000 Buried
214 N H -2.0389 Soluble
215 H H 0.0000 Buried
216 K H -2.7802 Soluble
217 P H -1.6167 Soluble
218 S H -1.8238 Soluble
219 N H -2.6197 Soluble
220 T H -2.0916 Soluble
221 K H -2.6857 Soluble
222 V H -1.4449 Soluble
223 D H -2.4501 Soluble
224 K H -1.8503 Soluble
225 K H -2.3361 Soluble
226 V H 0.0000 Buried
227 E H -2.7465 Soluble
228 P H -1.9123 Soluble
229 K H -2.4355 Soluble
1 D L -1.7053 Soluble
2 N L -0.8880 Soluble
3 V L 0.5101 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.7168 Soluble
6 Q L 0.0000 Buried
7 S L -1.4259 Soluble
8 P L -1.3671 Soluble
9 D L -2.4861 Soluble
10 S L -1.7222 Soluble
11 L L -0.9801 Soluble
12 A L -0.8623 Soluble
13 V L 0.0000 Buried
14 S L -1.4391 Soluble
15 L L -0.6340 Soluble
16 G L -1.4192 Soluble
17 E L -2.8057 Soluble
18 R L -2.9248 Soluble
19 A L 0.0000 Buried
20 T L -0.5479 Soluble
21 I L 0.0000 Buried
22 S L -1.0821 Soluble
23 C L 0.0000 Buried
24 R L -2.4405 Soluble
25 A L 0.0000 Buried
26 S L -1.3420 Soluble
27 K L -2.5109 Soluble
28 S L -2.1499 Soluble
29 V L 0.0000 Buried
30 R L -2.0496 Soluble
31 T L -0.9107 Soluble
34 S L -0.4528 Soluble
35 G L -0.4170 Soluble
36 Y L 0.2847 Aggregation-prone
37 N L -0.5947 Soluble
38 Y L -0.5660 Soluble
39 M L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.3889 Soluble
45 K L -1.8343 Soluble
46 P L -1.4659 Soluble
47 G L -1.4748 Soluble
48 Q L -2.1096 Soluble
49 P L -1.4264 Soluble
50 P L 0.0000 Buried
51 K L -1.5332 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.0718 Soluble
56 L L 0.0000 Buried
57 A L 0.0000 Buried
65 S L -0.5317 Soluble
66 N L -0.4387 Soluble
67 L L -0.4755 Soluble
68 K L -0.7143 Soluble
69 S L -0.6947 Soluble
70 G L -0.7598 Soluble
71 V L -0.8034 Soluble
72 P L -1.1512 Soluble
74 D L -2.0943 Soluble
75 R L -1.6292 Soluble
76 F L 0.0000 Buried
77 S L -0.7898 Soluble
78 G L -0.3727 Soluble
79 S L -0.7897 Soluble
80 G L -1.2248 Soluble
83 S L -1.2062 Soluble
84 G L -1.7490 Soluble
85 T L -2.3009 Soluble
86 D L -2.5726 Soluble
87 F L 0.0000 Buried
88 T L -0.8400 Soluble
89 L L 0.0000 Buried
90 T L -0.6511 Soluble
91 I L 0.0000 Buried
92 S L -1.9531 Soluble
93 S L -1.8721 Soluble
94 L L 0.0000 Buried
95 Q L -1.3028 Soluble
96 A L -0.9014 Soluble
97 E L -1.9891 Soluble
98 D L 0.0000 Buried
99 A L -0.9890 Soluble
100 A L -1.0950 Soluble
101 T L -1.3087 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 H L 0.0000 Buried
107 S L 0.0000 Buried
108 R L -1.6069 Soluble
109 E L -1.7012 Soluble
114 L L -0.1629 Soluble
115 P L 0.0000 Buried
116 Y L -0.0854 Soluble
117 T L -0.1644 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.3141 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -2.3838 Soluble
124 L L 0.0000 Buried
125 E L -1.2028 Soluble
126 I L -1.0686 Soluble
127 K L -1.6643 Soluble
128 R L -1.1597 Soluble
129 T L -0.0890 Soluble
130 V L 0.6663 Aggregation-prone
131 A L 0.1756 Aggregation-prone
132 A L 0.0101 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.1709 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.7891 Soluble
140 P L 0.0000 Buried
141 S L 0.0000 Buried
142 D L -3.0460 Soluble
143 E L -3.2291 Soluble
144 Q L 0.0000 Buried
145 L L -2.3579 Soluble
146 K L -2.9234 Soluble
147 S L -1.8230 Soluble
148 G L -1.3108 Soluble
149 T L -1.0703 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8745 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5715 Soluble
162 R L -2.7264 Soluble
163 E L -3.0506 Soluble
164 A L -2.2776 Soluble
165 K L -2.5960 Soluble
166 V L -1.2229 Soluble
167 Q L -0.6730 Soluble
168 W L 0.0000 Buried
169 K L -0.4206 Soluble
170 V L 0.0000 Buried
171 D L -1.5055 Soluble
172 N L -1.3266 Soluble
173 A L -0.1179 Soluble
174 L L 0.7621 Aggregation-prone
175 Q L -0.3378 Soluble
176 S L -0.7151 Soluble
177 G L -1.3343 Soluble
178 N L -1.6917 Soluble
179 S L -1.6235 Soluble
180 Q L -1.6316 Soluble
181 E L -2.1917 Soluble
182 S L -0.9006 Soluble
183 V L -1.0810 Soluble
184 T L -1.2227 Soluble
185 E L -2.3086 Soluble
186 Q L -1.9627 Soluble
187 D L -1.9876 Soluble
188 S L -1.8913 Soluble
189 K L -2.2831 Soluble
190 D L -1.7074 Soluble
191 S L -1.8417 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8659 Soluble
199 L L 0.0000 Buried
200 T L -0.6922 Soluble
201 L L -0.8357 Soluble
202 S L -1.0659 Soluble
203 K L -2.1175 Soluble
204 A L -1.8755 Soluble
205 D L -2.4640 Soluble
206 Y L 0.0000 Buried
207 E L -3.3506 Soluble
208 K L -3.5025 Soluble
209 H L -2.8418 Soluble
210 K L -2.8841 Soluble
211 V L -1.3135 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.4743 Soluble
216 V L 0.0000 Buried
217 T L -1.1692 Soluble
218 H L 0.0000 Buried
219 Q L -1.7418 Soluble
220 G L -0.4325 Soluble
221 L L -0.1286 Soluble
222 S L -0.4047 Soluble
223 S L -0.3348 Soluble
224 P L -0.4233 Soluble
225 V L 0.3573 Aggregation-prone
226 T L -0.1869 Soluble
227 K L -0.4642 Soluble
228 S L -0.5470 Soluble
229 F L 0.0000 Buried
230 N L -1.5987 Soluble
231 R L -2.3692 Soluble
232 G L -1.9194 Soluble
233 E L -2.2288 Soluble
234 C L -0.8549 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8239 1.56 CSV PDB
4.5 -0.8766 1.4984 CSV PDB
5.0 -0.9392 1.4067 CSV PDB
5.5 -0.9992 1.335 CSV PDB
6.0 -1.0428 1.3411 CSV PDB
6.5 -1.0586 1.3773 CSV PDB
7.0 -1.0459 1.4391 CSV PDB
7.5 -1.014 1.5169 CSV PDB
8.0 -0.9716 1.6018 CSV PDB
8.5 -0.9217 1.6883 CSV PDB
9.0 -0.8641 1.7729 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH