Utomilumab / 6mi2

A4DAB286_6mi2_D_E | Source PDB: 6mi2 | Chains: D, E | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6798
Max Score
1.5399
Min Score
-3.3692
Total Score
-285.53
Score CDR
-0.1389
Score non-CDR
-0.7447

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGESLRISCKGSGYSFSTYWISWVRQMPGKGLEWMGKIYPGDSYTNYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARGYGIFDYWGQGTLVTVSSASTKGPSVFPLAPSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVLVIYQDKNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYTGFGSLAVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP input.pdb
Selected Chain(s) D (Heavy), E (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3692
Maximum Score 1.5399
Average Score -0.6798
Total Score -285.5255

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E D -2.0319 Soluble
2 V D -1.1028 Soluble
3 Q D -1.6166 Soluble
4 L D 0.0000 Buried
5 V D -0.5257 Soluble
6 Q D 0.0000 Buried
7 S D -0.6943 Soluble
8 G D -0.5532 Soluble
9 A D 0.0678 Aggregation-prone
11 E D -0.3764 Soluble
12 V D 0.3598 Aggregation-prone
13 K D -1.0974 Soluble
14 K D -2.1166 Soluble
15 P D -1.9639 Soluble
16 G D -1.7801 Soluble
17 E D -1.6507 Soluble
18 S D -1.6353 Soluble
19 L D 0.0000 Buried
20 R D -2.1456 Soluble
21 I D 0.0000 Buried
22 S D -0.7548 Soluble
23 C D 0.0000 Buried
24 K D -1.0786 Soluble
25 G D 0.0000 Buried
26 S D -0.8272 Soluble
27 G D -1.1383 Soluble
28 Y D -0.4234 Soluble
29 S D -0.2889 Soluble
30 F D 0.0000 Buried
35 S D -0.7186 Soluble
36 T D -0.0400 Soluble
37 Y D 0.9163 Aggregation-prone
38 W D 1.0512 Aggregation-prone
39 I D 0.0000 Buried
40 S D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D -0.7178 Soluble
44 Q D -0.8513 Soluble
45 M D -0.8658 Soluble
46 P D -0.8956 Soluble
47 G D -1.4298 Soluble
48 K D -2.2262 Soluble
49 G D -1.4524 Soluble
50 L D 0.0000 Buried
51 E D -1.0707 Soluble
52 W D 0.0000 Buried
53 M D 0.0000 Buried
54 G D 0.0000 Buried
55 K D 0.0000 Buried
56 I D 0.0000 Buried
57 Y D 0.4606 Aggregation-prone
58 P D 0.0000 Buried
59 G D -0.9073 Soluble
62 D D -1.3004 Soluble
63 S D -0.5659 Soluble
64 Y D 0.8277 Aggregation-prone
65 T D 0.4125 Aggregation-prone
66 N D -0.0826 Soluble
67 Y D -0.5982 Soluble
68 S D 0.0000 Buried
69 P D -0.9494 Soluble
70 S D -0.9271 Soluble
71 F D 0.0000 Buried
72 Q D -1.8001 Soluble
74 G D -1.4971 Soluble
75 Q D -1.7656 Soluble
76 V D 0.0000 Buried
77 T D -0.8978 Soluble
78 I D 0.0000 Buried
79 S D -0.4376 Soluble
80 A D -0.7533 Soluble
81 D D -1.3012 Soluble
82 K D -1.9593 Soluble
83 S D -0.3392 Soluble
84 I D 0.9055 Aggregation-prone
85 S D -0.4039 Soluble
86 T D 0.0000 Buried
87 A D 0.0000 Buried
88 Y D -0.7265 Soluble
89 L D 0.0000 Buried
90 Q D -1.3262 Soluble
91 W D 0.0000 Buried
92 S D -1.2127 Soluble
93 S D -1.4258 Soluble
94 L D 0.0000 Buried
95 K D -2.3383 Soluble
96 A D -1.2867 Soluble
97 S D -0.6985 Soluble
98 D D 0.0000 Buried
99 T D -0.1220 Soluble
100 A D 0.0000 Buried
101 M D 0.4312 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 G D 0.8432 Aggregation-prone
108 Y D 1.3716 Aggregation-prone
109 G D 0.0000 Buried
114 I D 1.0174 Aggregation-prone
115 F D 0.0000 Buried
116 D D -0.1454 Soluble
117 Y D 0.2165 Aggregation-prone
118 W D -0.1056 Soluble
119 G D 0.0000 Buried
120 Q D -1.3593 Soluble
121 G D -0.5793 Soluble
122 T D 0.0000 Buried
123 L D 0.7448 Aggregation-prone
124 V D 0.0000 Buried
125 T D 0.1197 Aggregation-prone
126 V D 0.0000 Buried
127 S D -0.8859 Soluble
128 S D -0.9614 Soluble
129 A D -0.8298 Soluble
130 S D -0.8536 Soluble
131 T D -1.1597 Soluble
132 K D -2.0642 Soluble
133 G D -1.7914 Soluble
134 P D -0.8828 Soluble
135 S D -0.4322 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D 0.0000 Buried
139 L D 0.0000 Buried
140 A D -1.2100 Soluble
141 P D 0.0000 Buried
142 S D -0.5796 Soluble
143 T D -0.2356 Soluble
144 A D -0.2095 Soluble
145 A D -0.1213 Soluble
146 L D 0.0000 Buried
147 G D 0.0000 Buried
148 C D 0.0000 Buried
149 L D 0.0000 Buried
150 V D 0.0000 Buried
151 K D -0.4198 Soluble
152 D D -0.8204 Soluble
153 Y D 0.0000 Buried
154 F D 0.0000 Buried
155 P D -0.9524 Soluble
156 E D -1.2629 Soluble
157 P D -1.0630 Soluble
158 V D -0.7968 Soluble
159 T D -0.6888 Soluble
160 V D -0.3370 Soluble
161 S D -0.3354 Soluble
162 W D 0.0000 Buried
163 N D -0.6673 Soluble
164 S D -0.6469 Soluble
165 G D -0.5397 Soluble
166 A D -0.2105 Soluble
167 L D 0.0438 Aggregation-prone
168 T D -0.1876 Soluble
169 S D -0.4660 Soluble
170 G D -0.6416 Soluble
171 V D -0.1649 Soluble
172 H D -0.5449 Soluble
173 T D -0.0223 Soluble
174 F D 0.0000 Buried
175 P D -0.1474 Soluble
176 A D -0.1049 Soluble
177 V D 0.0000 Buried
178 L D 0.2262 Aggregation-prone
179 Q D -0.4527 Soluble
180 S D -0.4655 Soluble
181 S D -0.3285 Soluble
182 G D 0.0000 Buried
183 L D -0.4148 Soluble
184 Y D 0.0000 Buried
185 S D 0.0000 Buried
186 L D 0.0000 Buried
187 S D 0.0000 Buried
188 S D 0.0000 Buried
189 V D 0.0000 Buried
190 V D 0.0000 Buried
191 T D -0.2468 Soluble
192 V D 0.0000 Buried
193 P D -0.3575 Soluble
194 S D -0.4669 Soluble
195 S D -0.4602 Soluble
196 S D -0.6923 Soluble
197 L D -0.8807 Soluble
198 G D -1.1197 Soluble
199 T D -0.8939 Soluble
200 Q D -1.5538 Soluble
201 T D -1.2174 Soluble
202 Y D 0.0000 Buried
203 I D -1.0078 Soluble
204 C D 0.0000 Buried
205 N D -1.3770 Soluble
206 V D 0.0000 Buried
207 N D -2.0680 Soluble
208 H D 0.0000 Buried
209 K D -2.8147 Soluble
210 P D -1.8577 Soluble
211 S D -2.0821 Soluble
212 N D -2.7210 Soluble
213 T D -2.1590 Soluble
214 K D -2.6277 Soluble
215 V D -1.3936 Soluble
216 D D -2.1277 Soluble
217 K D -1.8749 Soluble
218 K D -2.0404 Soluble
219 V D 0.0000 Buried
220 E D -2.7768 Soluble
221 P D -1.9820 Soluble
222 K D -2.2809 Soluble
1 S E -0.5251 Soluble
2 Y E -0.8877 Soluble
3 E E -2.0219 Soluble
4 L E 0.0000 Buried
5 T E -0.7342 Soluble
6 Q E 0.0000 Buried
7 P E -0.4540 Soluble
8 P E -0.7234 Soluble
9 S E -0.6637 Soluble
11 V E -0.1556 Soluble
12 S E -0.2597 Soluble
13 V E -0.4306 Soluble
14 S E -0.7216 Soluble
15 P E -0.9471 Soluble
16 G E -1.4373 Soluble
17 Q E -1.7341 Soluble
18 T E -1.0086 Soluble
19 A E 0.0000 Buried
20 S E -0.3210 Soluble
21 I E 0.0000 Buried
22 T E -0.3083 Soluble
23 C E 0.0000 Buried
24 S E -1.4963 Soluble
25 G E -2.2607 Soluble
26 D E -3.3666 Soluble
27 N E -3.3692 Soluble
28 I E 0.0000 Buried
29 G E -2.3126 Soluble
36 D E -2.5396 Soluble
37 Q E -0.8312 Soluble
38 Y E 0.5802 Aggregation-prone
39 A E 0.0000 Buried
40 H E 0.0000 Buried
41 W E 0.0000 Buried
42 Y E 0.0000 Buried
43 Q E -0.1227 Soluble
44 Q E 0.0000 Buried
45 K E -1.3848 Soluble
46 P E -1.3562 Soluble
47 G E -1.5058 Soluble
48 Q E -1.7510 Soluble
49 S E -0.7474 Soluble
50 P E 0.0000 Buried
51 V E 1.3539 Aggregation-prone
52 L E 0.8627 Aggregation-prone
53 V E 0.0000 Buried
54 I E 0.0000 Buried
55 Y E -0.4511 Soluble
56 Q E -0.5548 Soluble
57 D E -1.2168 Soluble
65 K E -2.2981 Soluble
66 N E -1.8801 Soluble
67 R E -2.0707 Soluble
68 P E -1.0607 Soluble
69 S E -0.8731 Soluble
70 G E -0.8318 Soluble
71 I E -0.5429 Soluble
72 P E -1.2256 Soluble
74 E E -2.2341 Soluble
75 R E -1.4829 Soluble
76 F E 0.0000 Buried
77 S E -1.3936 Soluble
78 G E -1.4539 Soluble
79 S E -1.1558 Soluble
80 N E -1.3078 Soluble
83 S E -1.5510 Soluble
84 G E -2.2960 Soluble
85 N E -2.9687 Soluble
86 T E -1.5228 Soluble
87 A E 0.0000 Buried
88 T E -0.5149 Soluble
89 L E 0.0000 Buried
90 T E -0.3183 Soluble
91 I E 0.0000 Buried
92 S E -1.2097 Soluble
93 G E -1.2809 Soluble
94 T E 0.0000 Buried
95 Q E -0.9138 Soluble
96 A E -0.2356 Soluble
97 M E 0.4244 Aggregation-prone
98 D E 0.0000 Buried
99 E E -1.1330 Soluble
100 A E 0.0000 Buried
101 D E -1.2737 Soluble
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 A E 0.0000 Buried
106 T E 0.0000 Buried
107 Y E 1.0889 Aggregation-prone
108 T E 0.4967 Aggregation-prone
109 G E 0.4164 Aggregation-prone
110 F E 1.5399 Aggregation-prone
113 G E 0.3562 Aggregation-prone
114 S E 0.4659 Aggregation-prone
115 L E 0.5593 Aggregation-prone
116 A E 0.0000 Buried
117 V E -0.2205 Soluble
118 F E 0.0000 Buried
119 G E 0.0000 Buried
120 G E -1.2269 Soluble
121 G E -1.0307 Soluble
122 T E 0.0000 Buried
123 K E -1.4780 Soluble
124 L E 0.0000 Buried
125 T E -0.6158 Soluble
126 V E 0.0000 Buried
127 L E -0.6236 Soluble
128 G E -0.8463 Soluble
129 Q E -1.2398 Soluble
130 P E -1.2950 Soluble
131 K E -2.1478 Soluble
132 A E -1.3483 Soluble
133 A E -0.8312 Soluble
134 P E -0.4884 Soluble
135 S E -0.6932 Soluble
136 V E 0.0000 Buried
137 T E -0.6639 Soluble
138 L E 0.0000 Buried
139 F E 0.0000 Buried
140 P E -0.0654 Soluble
141 P E 0.0000 Buried
142 S E 0.0000 Buried
143 S E -1.1549 Soluble
144 E E -1.7082 Soluble
145 E E 0.0000 Buried
146 L E -1.3451 Soluble
147 Q E -2.0415 Soluble
148 A E -1.7155 Soluble
149 N E -2.3612 Soluble
150 K E -2.3228 Soluble
151 A E 0.0000 Buried
152 T E 0.0000 Buried
153 L E 0.0000 Buried
154 V E 0.0000 Buried
155 C E 0.0000 Buried
156 L E 0.0000 Buried
157 I E 0.0000 Buried
158 S E -0.7562 Soluble
159 D E -1.5288 Soluble
160 F E 0.0000 Buried
161 Y E 0.0000 Buried
162 P E -0.9855 Soluble
163 G E -0.8069 Soluble
164 A E -0.1987 Soluble
165 V E -0.0583 Soluble
166 T E -0.0302 Soluble
167 V E 0.0956 Aggregation-prone
168 A E -0.4942 Soluble
169 W E 0.0000 Buried
170 K E -1.1832 Soluble
171 A E 0.0000 Buried
172 D E -1.1958 Soluble
173 S E -0.6332 Soluble
174 S E -0.7080 Soluble
175 P E -0.7853 Soluble
176 V E -0.6410 Soluble
177 K E -1.7295 Soluble
178 A E -0.9783 Soluble
179 G E -1.0187 Soluble
180 V E -0.9674 Soluble
181 E E -1.3396 Soluble
182 T E -0.4919 Soluble
183 T E -0.3308 Soluble
184 T E -0.3380 Soluble
185 P E -0.5022 Soluble
186 S E -1.1466 Soluble
187 K E -2.5722 Soluble
188 Q E -2.0454 Soluble
189 S E -1.8112 Soluble
190 N E -2.4698 Soluble
191 N E -2.6043 Soluble
192 K E -2.1808 Soluble
193 Y E -1.5020 Soluble
194 A E 0.0000 Buried
195 A E 0.0000 Buried
196 S E 0.0000 Buried
197 S E 0.0000 Buried
198 Y E 0.0000 Buried
199 L E 0.0000 Buried
200 S E -0.7044 Soluble
201 L E 0.0000 Buried
202 T E -1.8649 Soluble
203 P E -2.4861 Soluble
204 E E -3.2059 Soluble
205 Q E -2.6870 Soluble
206 W E 0.0000 Buried
207 K E -3.1975 Soluble
208 S E -2.5228 Soluble
209 H E -2.4798 Soluble
210 R E -2.6336 Soluble
211 S E -1.4849 Soluble
212 Y E 0.0000 Buried
213 S E -1.2709 Soluble
214 C E 0.0000 Buried
215 Q E -1.1728 Soluble
216 V E 0.0000 Buried
217 T E -0.6103 Soluble
218 H E 0.0000 Buried
219 E E -2.2806 Soluble
220 G E -1.4319 Soluble
221 S E -0.8953 Soluble
222 T E -0.6897 Soluble
223 V E -0.8484 Soluble
224 E E -2.4944 Soluble
225 K E -2.3810 Soluble
226 T E -1.2510 Soluble
227 V E -0.4250 Soluble
228 A E -0.7296 Soluble
229 P E -1.0437 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6379 4.2145 CSV PDB
4.5 -0.6743 4.2145 CSV PDB
5.0 -0.7161 4.2145 CSV PDB
5.5 -0.7528 4.2145 CSV PDB
6.0 -0.7731 4.2145 CSV PDB
6.5 -0.768 4.2145 CSV PDB
7.0 -0.7382 4.2145 CSV PDB
7.5 -0.6924 4.2145 CSV PDB
8.0 -0.6384 4.2145 CSV PDB
8.5 -0.579 4.2145 CSV PDB
9.0 -0.5153 4.2145 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH