Vilastobart / 8g8n

A4DAB294_8g8n_H_L | Source PDB: 8g8n | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.8108
Max Score
1.233
Min Score
-4.0172
Total Score
-346.22
Score CDR
-0.6241
Score non-CDR
-0.8328

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGSSVKVSCKASGYTFTNYFMNWVRQAPGQGLEWMGRVDPEQGRADYAEKFKKRVTITADKSTSTAYMELSSLRSEDTAVYYCARRAMDNYGFAYWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKEIVLTQSPDFQSVTPKEKVTITCSANSALSYMYWYQQKPDQSPKLWVHGTSNLASGVPSRFSGSGSGTDFTLTINSLEAEDAATYYCHHWSNTQWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.0172
Maximum Score 1.233
Average Score -0.8108
Total Score -346.219

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.5976 Soluble
2 V H 0.0000 Buried
3 Q H -1.1786 Soluble
4 L H 0.0000 Buried
5 V H 0.6702 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.5294 Soluble
8 G H -0.6600 Soluble
9 A H -0.7632 Soluble
11 E H -1.1533 Soluble
12 V H -0.6455 Soluble
13 K H -1.6791 Soluble
14 K H -2.4675 Soluble
15 P H -2.2957 Soluble
16 G H -1.6154 Soluble
17 S H -1.2862 Soluble
18 S H -1.3839 Soluble
19 V H 0.0000 Buried
20 K H -2.0511 Soluble
21 V H 0.0000 Buried
22 S H -0.4050 Soluble
23 C H 0.0000 Buried
24 K H -0.5933 Soluble
25 A H 0.0000 Buried
26 S H -0.8096 Soluble
27 G H -1.1161 Soluble
28 Y H -0.6451 Soluble
29 T H -0.6315 Soluble
30 F H 0.0000 Buried
35 T H -1.9312 Soluble
36 N H -1.5312 Soluble
37 Y H -0.5436 Soluble
38 F H -0.4416 Soluble
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.6357 Soluble
45 A H -0.9833 Soluble
46 P H -0.9953 Soluble
47 G H -1.2524 Soluble
48 Q H -1.7415 Soluble
49 G H -1.1701 Soluble
50 L H 0.0000 Buried
51 E H -0.9294 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 R H 0.0000 Buried
56 V H 0.0000 Buried
57 D H -1.9465 Soluble
58 P H 0.0000 Buried
59 E H -3.5088 Soluble
62 Q H -3.0865 Soluble
63 G H -2.7975 Soluble
64 R H -2.8334 Soluble
65 A H -1.3387 Soluble
66 D H -1.3091 Soluble
67 Y H -1.5977 Soluble
68 A H 0.0000 Buried
69 E H -3.5191 Soluble
70 K H -3.4432 Soluble
71 F H 0.0000 Buried
72 K H -3.6985 Soluble
74 K H -3.3100 Soluble
75 R H -2.0751 Soluble
76 V H 0.0000 Buried
77 T H -1.0849 Soluble
78 I H 0.0000 Buried
79 T H -0.7589 Soluble
80 A H -1.6118 Soluble
81 D H -2.0813 Soluble
82 K H -2.7906 Soluble
83 S H -1.3470 Soluble
84 T H -1.1299 Soluble
85 S H -1.2922 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.4276 Soluble
89 M H 0.0000 Buried
90 E H -1.4180 Soluble
91 L H 0.0000 Buried
92 S H -1.3167 Soluble
93 S H -1.3447 Soluble
94 L H 0.0000 Buried
95 R H -3.1310 Soluble
96 S H -2.3970 Soluble
97 E H -2.5350 Soluble
98 D H 0.0000 Buried
99 T H -0.9551 Soluble
100 A H 0.0000 Buried
101 V H 0.2024 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 R H 0.0023 Aggregation-prone
108 A H 0.0000 Buried
109 M H 0.2225 Aggregation-prone
110 D H -1.2610 Soluble
112 N H -0.4545 Soluble
113 Y H 0.6294 Aggregation-prone
114 G H 0.0000 Buried
115 F H 0.0000 Buried
116 A H -0.1426 Soluble
117 Y H -0.1374 Soluble
118 W H -0.3311 Soluble
119 G H 0.0000 Buried
120 Q H -1.3213 Soluble
121 G H -0.6930 Soluble
122 T H 0.0000 Buried
123 L H -0.1722 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.3660 Soluble
128 S H -1.4330 Soluble
129 A H -0.6119 Soluble
130 S H -0.6361 Soluble
131 T H -0.6991 Soluble
132 K H -1.2227 Soluble
133 G H -1.4425 Soluble
134 P H -0.6781 Soluble
135 S H -0.2597 Soluble
136 V H 0.0000 Buried
137 F H -0.5203 Soluble
138 P H -1.0588 Soluble
139 L H 0.0000 Buried
140 A H -1.0675 Soluble
141 P H 0.0000 Buried
142 S H -0.6776 Soluble
143 G H -0.6487 Soluble
144 T H -0.4828 Soluble
145 A H -0.2587 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.4499 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.3426 Soluble
158 P H -0.7482 Soluble
159 V H -0.8300 Soluble
160 T H -0.7709 Soluble
161 V H -0.5310 Soluble
162 S H -0.5179 Soluble
163 W H 0.0000 Buried
164 N H -0.8106 Soluble
165 S H -0.7085 Soluble
166 G H -0.5859 Soluble
167 A H -0.2814 Soluble
168 L H -0.0417 Soluble
169 T H -0.2080 Soluble
170 S H -0.1991 Soluble
171 G H -0.2359 Soluble
172 V H 0.1165 Aggregation-prone
173 H H -0.3364 Soluble
174 T H -0.1377 Soluble
175 F H 0.0000 Buried
176 P H -0.2955 Soluble
177 A H 0.2400 Aggregation-prone
178 V H 0.3886 Aggregation-prone
179 L H 1.0483 Aggregation-prone
180 Q H 0.2233 Aggregation-prone
181 S H -0.0824 Soluble
182 S H -0.2157 Soluble
183 G H 0.0539 Aggregation-prone
184 L H 0.1332 Aggregation-prone
185 Y H 0.3999 Aggregation-prone
186 S H 0.2724 Aggregation-prone
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1513 Soluble
193 V H 0.0000 Buried
194 P H -0.6276 Soluble
195 S H -0.5659 Soluble
196 S H -0.6520 Soluble
197 S H -0.7023 Soluble
198 L H -0.8962 Soluble
199 G H -1.0413 Soluble
200 T H -0.8598 Soluble
201 Q H -1.5182 Soluble
202 T H -1.2914 Soluble
203 Y H 0.0000 Buried
204 I H -1.2427 Soluble
205 C H 0.0000 Buried
206 N H -1.6746 Soluble
207 V H 0.0000 Buried
208 N H -2.5661 Soluble
209 H H 0.0000 Buried
210 K H -2.9086 Soluble
211 P H -1.8212 Soluble
212 S H -1.9606 Soluble
213 N H -2.6952 Soluble
214 T H -2.2684 Soluble
215 K H -2.9410 Soluble
216 V H -1.8088 Soluble
217 D H -2.6401 Soluble
218 K H -1.9197 Soluble
219 K H -2.3407 Soluble
220 V H 0.0000 Buried
221 E H -2.6926 Soluble
222 P H -1.5234 Soluble
223 K H -1.8970 Soluble
1 E L -1.4856 Soluble
2 I L -0.2595 Soluble
3 V L 1.0171 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.3820 Soluble
6 Q L -0.8214 Soluble
7 S L -0.9176 Soluble
8 P L -0.9639 Soluble
9 D L -1.7034 Soluble
10 F L -0.0789 Soluble
11 Q L -0.5644 Soluble
12 S L -0.5482 Soluble
13 V L 0.0000 Buried
14 T L -2.0357 Soluble
15 P L -2.5668 Soluble
16 K L -3.5582 Soluble
17 E L -4.0172 Soluble
18 K L -3.6170 Soluble
19 V L 0.0000 Buried
20 T L -0.7378 Soluble
21 I L 0.0000 Buried
22 T L -0.7279 Soluble
23 C L 0.0000 Buried
24 S L -0.8051 Soluble
25 A L -0.7085 Soluble
26 N L -1.3562 Soluble
27 S L -0.8619 Soluble
28 A L -0.5573 Soluble
29 L L -0.1087 Soluble
37 S L 0.4220 Aggregation-prone
38 Y L 1.2330 Aggregation-prone
39 M L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.7850 Soluble
46 P L -2.0385 Soluble
47 D L -2.7696 Soluble
48 Q L -2.6297 Soluble
49 S L -1.5236 Soluble
50 P L 0.0000 Buried
51 K L -1.0834 Soluble
52 L L 0.0000 Buried
53 W L 0.0000 Buried
54 V L 0.0000 Buried
55 H L -0.3525 Soluble
56 G L 0.0303 Aggregation-prone
57 T L -0.2147 Soluble
65 S L -0.7412 Soluble
66 N L -1.2900 Soluble
67 L L -0.4408 Soluble
68 A L -0.4590 Soluble
69 S L -0.4351 Soluble
70 G L -0.4604 Soluble
71 V L -0.4065 Soluble
72 P L -0.3579 Soluble
74 S L -0.6314 Soluble
75 R L -1.3962 Soluble
76 F L 0.0000 Buried
77 S L -0.7890 Soluble
78 G L -0.6339 Soluble
79 S L -0.6869 Soluble
80 G L -0.7890 Soluble
83 S L -0.7496 Soluble
84 G L -0.9449 Soluble
85 T L -1.2645 Soluble
86 D L -1.8837 Soluble
87 F L 0.0000 Buried
88 T L -0.6184 Soluble
89 L L 0.0000 Buried
90 T L -0.9195 Soluble
91 I L 0.0000 Buried
92 N L -3.1948 Soluble
93 S L -3.0511 Soluble
94 L L 0.0000 Buried
95 E L -2.5254 Soluble
96 A L -2.1437 Soluble
97 E L -2.4259 Soluble
98 D L 0.0000 Buried
99 A L -1.2123 Soluble
100 A L 0.0000 Buried
101 T L -0.8647 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 H L 0.0000 Buried
106 H L 0.0000 Buried
107 W L 0.1573 Aggregation-prone
108 S L -0.6648 Soluble
109 N L -1.3290 Soluble
114 T L -1.1764 Soluble
115 Q L -0.9936 Soluble
116 W L 0.0000 Buried
117 T L 0.2126 Aggregation-prone
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.1568 Soluble
121 G L -1.1336 Soluble
122 T L 0.0000 Buried
123 K L -1.4313 Soluble
124 V L 0.0000 Buried
125 E L -1.1781 Soluble
126 I L 0.0000 Buried
127 K L -1.3120 Soluble
128 R L -1.0473 Soluble
129 T L -0.0357 Soluble
130 V L 0.5145 Aggregation-prone
131 A L 0.1802 Aggregation-prone
132 A L -0.1015 Soluble
133 P L 0.0000 Buried
134 S L -0.1374 Soluble
135 V L 0.0000 Buried
136 F L 0.1656 Aggregation-prone
137 I L 0.1501 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.8763 Soluble
140 P L -0.7825 Soluble
141 S L -1.5827 Soluble
142 D L -2.8320 Soluble
143 E L -2.5416 Soluble
144 Q L 0.0000 Buried
145 L L -2.2037 Soluble
146 K L -2.7198 Soluble
147 S L -1.7206 Soluble
148 G L -1.2424 Soluble
149 T L -1.1479 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9520 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7568 Soluble
162 R L -2.9590 Soluble
163 E L -3.2125 Soluble
164 A L -2.2473 Soluble
165 K L -2.0122 Soluble
166 V L -0.7980 Soluble
167 Q L -0.4114 Soluble
168 W L 0.0000 Buried
169 K L -0.5145 Soluble
170 V L 0.0000 Buried
171 D L -1.9175 Soluble
172 N L -1.5049 Soluble
173 A L -0.2519 Soluble
174 L L 0.7415 Aggregation-prone
175 Q L -0.3180 Soluble
176 S L -0.6654 Soluble
177 G L -1.3599 Soluble
178 N L -1.7289 Soluble
179 S L -1.4997 Soluble
180 Q L -1.5336 Soluble
181 E L -1.6481 Soluble
182 S L -0.8588 Soluble
183 V L -0.9382 Soluble
184 T L -1.1797 Soluble
185 E L -2.2731 Soluble
186 Q L -1.9524 Soluble
187 D L -1.9269 Soluble
188 S L -2.0511 Soluble
189 K L -2.4082 Soluble
190 D L -1.6879 Soluble
191 S L -1.7254 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7376 Soluble
199 L L 0.0000 Buried
200 T L -0.6607 Soluble
201 L L -0.8312 Soluble
202 S L -1.0684 Soluble
203 K L -2.1457 Soluble
204 A L -1.8897 Soluble
205 D L -2.4651 Soluble
206 Y L 0.0000 Buried
207 E L -3.7609 Soluble
208 K L -3.6400 Soluble
209 H L -3.1774 Soluble
210 K L -3.3374 Soluble
211 V L -1.5865 Soluble
212 Y L 0.0000 Buried
213 A L -0.8024 Soluble
214 C L 0.0000 Buried
215 E L -0.6782 Soluble
216 V L 0.0000 Buried
217 T L -1.0703 Soluble
218 H L 0.0000 Buried
219 Q L -1.7297 Soluble
220 G L -0.3826 Soluble
221 L L -0.2116 Soluble
222 S L -0.4573 Soluble
223 S L -0.4075 Soluble
224 P L -0.4094 Soluble
225 V L 0.1456 Aggregation-prone
226 T L -0.3737 Soluble
227 K L -0.6614 Soluble
228 S L -0.6315 Soluble
229 F L -1.1116 Soluble
230 N L -2.1768 Soluble
231 R L -2.8519 Soluble
232 G L -2.0770 Soluble
233 E L -2.2412 Soluble
234 C L -0.6199 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8108 2.1472 CSV PDB
4.5 -0.8635 1.9594 CSV PDB
5.0 -0.9261 1.7536 CSV PDB
5.5 -0.986 1.5471 CSV PDB
6.0 -1.0283 1.4852 CSV PDB
6.5 -1.0413 1.4746 CSV PDB
7.0 -1.0235 1.5543 CSV PDB
7.5 -0.9845 1.6404 CSV PDB
8.0 -0.9336 1.7284 CSV PDB
8.5 -0.8747 1.8162 CSV PDB
9.0 -0.8089 1.9011 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH