Pamlectabart / 8qyb

A4DAB201_8qyb_H_L | Source PDB: 8qyb | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6708
Max Score
1.2386
Min Score
-3.4326
Total Score
-285.1
Score CDR
-0.2619
Score non-CDR
-0.7217

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSRYWISWVRQAPGKGLVWVGEINPSSSTINYAPSLKDKFTISRDNAKNTLYLQMNSLRAEDTAVYYCASLYYDYGDAYDYWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPAEIVMTQSPATLSVSPGERATLSCKASQSVESNVAWYQQKPGQAPRALIYSASLRFSGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYNNYPLTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4326
Maximum Score 1.2386
Average Score -0.6708
Total Score -285.0968

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9387 Soluble
2 V H -0.8179 Soluble
3 Q H -1.0128 Soluble
4 L H 0.0000 Buried
5 V H 0.9825 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4309 Soluble
8 G H -0.9384 Soluble
9 G H -0.5419 Soluble
11 G H 0.0795 Aggregation-prone
12 L H 0.3040 Aggregation-prone
13 V H -0.5146 Soluble
14 Q H -1.5614 Soluble
15 P H -1.6902 Soluble
16 G H -1.4226 Soluble
17 G H -0.9564 Soluble
18 S H -1.1588 Soluble
19 L H -1.1112 Soluble
20 R H -2.2509 Soluble
21 L H 0.0000 Buried
22 S H -0.4629 Soluble
23 C H 0.0000 Buried
24 A H -0.2918 Soluble
25 A H 0.0000 Buried
26 S H -0.9052 Soluble
27 G H -0.9849 Soluble
28 F H -0.6006 Soluble
29 T H -0.7923 Soluble
30 F H 0.0000 Buried
35 S H -1.5309 Soluble
36 R H -1.8704 Soluble
37 Y H 0.0000 Buried
38 W H 0.2676 Aggregation-prone
39 I H 0.0000 Buried
40 S H 0.1819 Aggregation-prone
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.1071 Soluble
44 Q H -0.4934 Soluble
45 A H -1.0102 Soluble
46 P H -0.9801 Soluble
47 G H -1.4626 Soluble
48 K H -2.0275 Soluble
49 G H -0.9057 Soluble
50 L H 0.0000 Buried
51 V H 0.4310 Aggregation-prone
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 G H 0.0000 Buried
55 E H 0.0814 Aggregation-prone
56 I H 0.0000 Buried
57 N H 0.0000 Buried
58 P H -0.7657 Soluble
59 S H -0.6660 Soluble
62 S H -0.3007 Soluble
63 S H -0.1957 Soluble
64 T H 0.1175 Aggregation-prone
65 I H 0.6055 Aggregation-prone
66 N H 0.1790 Aggregation-prone
67 Y H -0.2955 Soluble
68 A H 0.0000 Buried
69 P H -1.2577 Soluble
70 S H -1.0814 Soluble
71 L H 0.0000 Buried
72 K H -2.7874 Soluble
74 D H -2.9436 Soluble
75 K H -2.3444 Soluble
76 F H 0.0000 Buried
77 T H -1.1637 Soluble
78 I H 0.0000 Buried
79 S H -0.3124 Soluble
80 R H 0.0000 Buried
81 D H -1.5887 Soluble
82 N H -1.9485 Soluble
83 A H -1.4044 Soluble
84 K H -2.3329 Soluble
85 N H -1.7835 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6306 Soluble
89 L H 0.0000 Buried
90 Q H -1.5980 Soluble
91 M H 0.0000 Buried
92 N H -1.6699 Soluble
93 S H -1.4222 Soluble
94 L H 0.0000 Buried
95 R H -2.5576 Soluble
96 A H -1.7654 Soluble
97 E H -2.3003 Soluble
98 D H 0.0000 Buried
99 T H -0.7298 Soluble
100 A H 0.0000 Buried
101 V H 0.1915 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H 0.0000 Buried
107 L H 1.1449 Aggregation-prone
108 Y H 0.7923 Aggregation-prone
109 Y H 1.2386 Aggregation-prone
110 D H 0.5724 Aggregation-prone
111 Y H 0.8620 Aggregation-prone
112 G H -0.2264 Soluble
113 D H -0.9827 Soluble
114 A H 0.0000 Buried
115 Y H 0.3451 Aggregation-prone
116 D H 0.3438 Aggregation-prone
117 Y H 0.2232 Aggregation-prone
118 W H -0.1739 Soluble
119 G H 0.0000 Buried
120 Q H -1.2070 Soluble
121 G H -0.5209 Soluble
122 T H -0.0516 Soluble
123 L H 0.1985 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.0386 Soluble
128 S H -0.8347 Soluble
129 A H -0.5106 Soluble
130 S H -0.6901 Soluble
131 T H -0.9237 Soluble
132 K H -1.8102 Soluble
133 G H -1.8336 Soluble
134 P H -1.0161 Soluble
135 S H -0.6655 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2053 Soluble
139 L H 0.0000 Buried
140 A H -0.6527 Soluble
141 P H -0.4933 Soluble
142 S H -0.2384 Soluble
143 G H -0.6357 Soluble
144 T H -0.5409 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.9520 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.2358 Soluble
158 P H -0.6394 Soluble
159 V H -0.5567 Soluble
160 T H -0.7895 Soluble
161 V H -0.5449 Soluble
162 S H -0.5540 Soluble
163 W H 0.0000 Buried
164 N H -0.9596 Soluble
165 S H -0.7428 Soluble
166 G H -0.6387 Soluble
167 A H -0.3038 Soluble
168 L H -0.0002 Soluble
169 T H -0.1366 Soluble
170 S H -0.1567 Soluble
171 G H -0.3200 Soluble
172 V H 0.1428 Aggregation-prone
173 H H -0.3387 Soluble
174 T H -0.2031 Soluble
175 F H 0.0000 Buried
176 P H -0.2901 Soluble
177 A H 0.2908 Aggregation-prone
178 V H 0.5450 Aggregation-prone
179 L H 1.0671 Aggregation-prone
180 Q H 0.1367 Aggregation-prone
181 S H -0.1164 Soluble
182 S H -0.3324 Soluble
183 G H -0.0878 Soluble
184 L H 0.0000 Buried
185 Y H 0.3896 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1568 Soluble
193 V H 0.0000 Buried
194 P H -0.5085 Soluble
195 S H -0.5968 Soluble
196 S H -0.6608 Soluble
197 S H -0.6935 Soluble
198 L H -0.9577 Soluble
199 G H -1.0763 Soluble
200 T H -0.8662 Soluble
201 Q H -1.5506 Soluble
202 T H -1.3249 Soluble
203 Y H 0.0000 Buried
204 I H -1.2928 Soluble
205 C H 0.0000 Buried
206 N H -1.8309 Soluble
207 V H 0.0000 Buried
208 N H -2.7163 Soluble
209 H H 0.0000 Buried
210 K H -2.8803 Soluble
211 P H -1.6872 Soluble
212 S H -2.0372 Soluble
213 N H -2.8411 Soluble
214 T H -2.3631 Soluble
215 K H -3.0185 Soluble
216 V H -1.8706 Soluble
217 D H -2.5546 Soluble
218 K H -2.1670 Soluble
219 R H -2.6731 Soluble
220 V H 0.0000 Buried
221 E H -2.4850 Soluble
222 P H -1.0723 Soluble
223 A H -0.4301 Soluble
1 E L -1.3861 Soluble
2 I L -0.1531 Soluble
3 V L 0.9750 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.2794 Soluble
6 Q L 0.0000 Buried
7 S L -0.4645 Soluble
8 P L -0.2012 Soluble
9 A L -0.0930 Soluble
10 T L -0.1471 Soluble
11 L L -0.0660 Soluble
12 S L -0.4019 Soluble
13 V L 0.0000 Buried
14 S L -1.3954 Soluble
15 P L -1.7132 Soluble
16 G L -1.7608 Soluble
17 E L -2.4688 Soluble
18 R L -2.6434 Soluble
19 A L 0.0000 Buried
20 T L -0.5189 Soluble
21 L L 0.0000 Buried
22 S L -0.8667 Soluble
23 C L 0.0000 Buried
24 K L -2.1600 Soluble
25 A L -1.3609 Soluble
26 S L -0.8404 Soluble
27 Q L -2.0026 Soluble
28 S L -2.1245 Soluble
29 V L 0.0000 Buried
36 E L -2.5630 Soluble
37 S L -1.5801 Soluble
38 N L -1.0279 Soluble
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6713 Soluble
46 P L -1.4317 Soluble
47 G L -1.5541 Soluble
48 Q L -2.1212 Soluble
49 A L -1.3342 Soluble
50 P L 0.0000 Buried
51 R L -1.1596 Soluble
52 A L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0507 Aggregation-prone
56 S L 0.0853 Aggregation-prone
57 A L -0.4352 Soluble
65 S L -0.0514 Soluble
66 L L 0.5886 Aggregation-prone
67 R L -1.2185 Soluble
68 F L 0.0000 Buried
69 S L -0.7275 Soluble
70 G L -0.7895 Soluble
71 I L -0.6246 Soluble
72 P L -0.5146 Soluble
74 A L -0.3541 Soluble
75 R L -0.6908 Soluble
76 F L 0.0000 Buried
77 S L -0.4330 Soluble
78 G L 0.0000 Buried
79 S L -0.5523 Soluble
80 G L -1.5267 Soluble
83 S L -1.8333 Soluble
84 G L -2.1037 Soluble
85 T L -2.2671 Soluble
86 E L -2.8395 Soluble
87 F L 0.0000 Buried
88 T L -0.8594 Soluble
89 L L 0.0000 Buried
90 T L -0.6054 Soluble
91 I L 0.0000 Buried
92 S L -1.3711 Soluble
93 S L -1.5669 Soluble
94 L L 0.0000 Buried
95 Q L -1.7653 Soluble
96 S L -1.6920 Soluble
97 E L -2.2363 Soluble
98 D L 0.0000 Buried
99 F L -0.6968 Soluble
100 A L 0.0000 Buried
101 V L -0.1027 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0921 Aggregation-prone
108 N L -0.9774 Soluble
109 N L -1.0539 Soluble
114 Y L 0.4806 Aggregation-prone
115 P L 0.2691 Aggregation-prone
116 L L 0.4300 Aggregation-prone
117 T L 0.5511 Aggregation-prone
118 F L 0.7708 Aggregation-prone
119 G L 0.0000 Buried
120 A L -0.0482 Soluble
121 G L -0.3243 Soluble
122 T L 0.0000 Buried
123 K L -0.4216 Soluble
124 L L 0.0000 Buried
125 E L -0.8698 Soluble
126 L L -0.7010 Soluble
127 K L -1.0004 Soluble
128 R L -0.6728 Soluble
129 T L 0.3572 Aggregation-prone
130 V L 0.9681 Aggregation-prone
131 A L 0.3810 Aggregation-prone
132 A L -0.0612 Soluble
133 P L 0.0000 Buried
134 S L -0.2818 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L -0.0677 Soluble
138 F L 0.0000 Buried
139 P L -0.6520 Soluble
140 P L -0.7350 Soluble
141 S L -1.6060 Soluble
142 D L -2.9099 Soluble
143 E L -3.0074 Soluble
144 Q L 0.0000 Buried
145 L L -2.1761 Soluble
146 K L -2.7422 Soluble
147 S L -1.7241 Soluble
148 G L -1.1557 Soluble
149 T L -0.7520 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9356 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L 0.0000 Buried
162 R L -2.8647 Soluble
163 E L -3.2908 Soluble
164 A L -2.4525 Soluble
165 K L -2.5588 Soluble
166 V L 0.0000 Buried
167 Q L -0.6646 Soluble
168 W L 0.0000 Buried
169 K L -0.6497 Soluble
170 V L 0.0000 Buried
171 D L -1.7594 Soluble
172 N L -1.4262 Soluble
173 A L -0.3558 Soluble
174 L L 0.6489 Aggregation-prone
175 Q L -0.2380 Soluble
176 S L -0.4310 Soluble
177 G L -0.9446 Soluble
178 N L -0.8765 Soluble
179 S L -1.1870 Soluble
180 Q L -1.4528 Soluble
181 E L -1.7385 Soluble
182 S L -0.7036 Soluble
183 V L -0.7105 Soluble
184 T L -0.9864 Soluble
185 E L -2.1894 Soluble
186 Q L -2.1568 Soluble
187 D L -2.4090 Soluble
188 S L -2.1510 Soluble
189 K L -2.4922 Soluble
190 D L -2.0951 Soluble
191 S L -2.0597 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6824 Soluble
199 L L 0.0000 Buried
200 T L -0.2967 Soluble
201 L L -0.4992 Soluble
202 S L -1.1104 Soluble
203 K L -1.8767 Soluble
204 A L -1.7324 Soluble
205 D L -2.2324 Soluble
206 Y L 0.0000 Buried
207 E L -3.1152 Soluble
208 K L -3.3737 Soluble
209 H L -3.0408 Soluble
210 K L -3.4326 Soluble
211 V L -1.8602 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8083 Soluble
216 V L 0.0000 Buried
217 T L -1.2025 Soluble
218 H L 0.0000 Buried
219 Q L -1.6759 Soluble
220 G L -0.3028 Soluble
221 L L -0.1536 Soluble
222 S L -0.4355 Soluble
223 S L -0.3726 Soluble
224 P L -0.4921 Soluble
225 V L 0.0776 Aggregation-prone
226 T L -0.6112 Soluble
227 K L -1.0393 Soluble
228 S L -1.0506 Soluble
229 F L -1.1880 Soluble
230 N L -2.4973 Soluble
231 R L -3.3037 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.691 2.0447 CSV PDB
4.5 -0.7337 1.9472 CSV PDB
5.0 -0.7854 1.8264 CSV PDB
5.5 -0.8364 1.6941 CSV PDB
6.0 -0.8759 1.5577 CSV PDB
6.5 -0.8939 1.5055 CSV PDB
7.0 -0.8881 1.4699 CSV PDB
7.5 -0.8656 1.4352 CSV PDB
8.0 -0.8338 1.4035 CSV PDB
8.5 -0.7961 1.3936 CSV PDB
9.0 -0.7529 1.5454 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH