Otilimab / 5c7x

A4DAB198_5c7x_H_L | Source PDB: 5c7x | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7095
Max Score
1.8916
Min Score
-3.3396
Total Score
-298.0
Score CDR
-0.5761
Score non-CDR
-0.7248

Calculation Parameters

Chain Sequence(s) QVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMNWVRQAPGKGLEWVSGIENKYAGGATYYAASVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGFGTDFWGQGTLVTVSSASTKGPSVFPLAPSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIELTQPPSVSVAPGQTARISCSGDSIGKKYAYWYQQKPGQAPVLVIYKKRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCSAWGDKGMVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3396
Maximum Score 1.8916
Average Score -0.7095
Total Score -298.0049

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.3190 Soluble
2 V H -0.4954 Soluble
3 Q H -0.7582 Soluble
4 L H 0.0000 Buried
5 V H 1.1696 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.2366 Soluble
8 G H -0.9788 Soluble
9 G H -0.7600 Soluble
11 G H -0.4233 Soluble
12 L H -0.1337 Soluble
13 V H 0.0000 Buried
14 Q H -1.6847 Soluble
15 P H -1.7202 Soluble
16 G H -1.4145 Soluble
17 G H -1.1219 Soluble
18 S H -1.2359 Soluble
19 L H -1.1590 Soluble
20 R H -2.2073 Soluble
21 L H 0.0000 Buried
22 S H -0.3924 Soluble
23 C H 0.0000 Buried
24 A H -0.0416 Soluble
25 A H 0.0000 Buried
26 S H -0.6768 Soluble
27 G H -0.8467 Soluble
28 F H -0.1813 Soluble
29 T H -0.0927 Soluble
30 F H 0.0000 Buried
35 S H -0.9857 Soluble
36 S H -0.1565 Soluble
37 Y H 0.6287 Aggregation-prone
38 W H 0.4554 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7952 Soluble
45 A H -1.1842 Soluble
46 P H -0.9680 Soluble
47 G H -1.4617 Soluble
48 K H -2.2719 Soluble
49 G H -1.5133 Soluble
50 L H 0.0000 Buried
51 E H -1.0065 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 E H -0.2293 Soluble
58 N H -0.7972 Soluble
59 K H -1.3948 Soluble
60 Y H 0.2834 Aggregation-prone
61 A H -0.1731 Soluble
62 G H -0.7733 Soluble
63 G H -0.6849 Soluble
64 A H -0.2625 Soluble
65 T H 0.3331 Aggregation-prone
66 Y H 0.5206 Aggregation-prone
67 Y H -0.2944 Soluble
68 A H -0.7492 Soluble
69 A H -0.8258 Soluble
70 S H -0.9460 Soluble
71 V H 0.0000 Buried
72 K H -1.8558 Soluble
74 G H -1.5432 Soluble
75 R H -1.4480 Soluble
76 F H 0.0000 Buried
77 T H -0.8071 Soluble
78 I H 0.0000 Buried
79 S H -0.4679 Soluble
80 R H -1.1526 Soluble
81 D H -1.8793 Soluble
82 N H -2.1442 Soluble
83 S H -1.7202 Soluble
84 K H -2.4787 Soluble
85 N H -1.8207 Soluble
86 T H -1.0327 Soluble
87 L H 0.0000 Buried
88 Y H -0.6799 Soluble
89 L H 0.0000 Buried
90 Q H -1.4416 Soluble
91 M H 0.0000 Buried
92 N H -1.5794 Soluble
93 S H -1.2568 Soluble
94 L H 0.0000 Buried
95 R H -2.2360 Soluble
96 A H -1.7200 Soluble
97 E H -2.2297 Soluble
98 D H 0.0000 Buried
99 T H -0.7450 Soluble
100 A H 0.0000 Buried
101 V H 0.2338 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 G H 0.0000 Buried
108 F H 1.8916 Aggregation-prone
114 G H 0.7214 Aggregation-prone
115 T H 0.0000 Buried
116 D H 0.8927 Aggregation-prone
117 F H 0.9628 Aggregation-prone
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.1863 Soluble
121 G H -0.5182 Soluble
122 T H -0.0409 Soluble
123 L H 0.0474 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4508 Soluble
126 V H 0.0000 Buried
127 S H -0.9882 Soluble
128 S H -0.7543 Soluble
129 A H -0.5048 Soluble
130 S H -0.6424 Soluble
131 T H -0.8255 Soluble
132 K H -1.5249 Soluble
133 G H -1.5605 Soluble
134 P H -0.6623 Soluble
135 S H -0.3130 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H 0.0000 Buried
139 L H 0.0000 Buried
140 A H -1.0940 Soluble
141 P H 0.0000 Buried
142 S H -0.6319 Soluble
143 S H -0.4764 Soluble
144 G H -0.7577 Soluble
145 G H -0.8683 Soluble
146 T H -0.6271 Soluble
147 A H 0.0000 Buried
148 A H -0.1355 Soluble
149 L H 0.0000 Buried
150 G H 0.0000 Buried
151 C H 0.0000 Buried
152 L H 0.0000 Buried
153 V H 0.0000 Buried
154 K H 0.0000 Buried
155 D H -0.3999 Soluble
156 Y H 0.0000 Buried
157 F H -0.2250 Soluble
158 P H 0.0000 Buried
159 E H -0.4360 Soluble
160 P H -0.7337 Soluble
161 V H 0.0000 Buried
162 T H -0.7430 Soluble
163 V H 0.0000 Buried
164 S H -0.4911 Soluble
165 W H 0.0000 Buried
166 N H -0.7659 Soluble
167 S H -0.7260 Soluble
168 G H -0.6374 Soluble
169 A H -0.2692 Soluble
170 L H -0.0274 Soluble
171 T H -0.1899 Soluble
172 S H -0.2857 Soluble
173 G H -0.3783 Soluble
174 V H 0.0845 Aggregation-prone
175 H H -0.1807 Soluble
176 T H -0.1118 Soluble
177 F H 0.0000 Buried
178 P H -0.0903 Soluble
179 A H 0.2205 Aggregation-prone
180 V H 0.0000 Buried
181 L H 1.1183 Aggregation-prone
182 Q H 0.0000 Buried
183 S H -0.0658 Soluble
184 S H -0.1294 Soluble
185 G H 0.1726 Aggregation-prone
186 L H 0.2206 Aggregation-prone
187 Y H 0.4265 Aggregation-prone
188 S H 0.0000 Buried
189 L H 0.0000 Buried
190 S H 0.0000 Buried
191 S H 0.0000 Buried
192 V H 0.0000 Buried
193 V H 0.0000 Buried
194 T H -0.2369 Soluble
195 V H 0.0000 Buried
196 P H -0.6706 Soluble
197 S H -0.7120 Soluble
198 S H -0.6912 Soluble
199 S H -0.7893 Soluble
200 L H -0.8528 Soluble
201 G H -1.1038 Soluble
202 T H -0.9002 Soluble
203 Q H -1.4092 Soluble
204 T H -1.0452 Soluble
205 Y H 0.0000 Buried
206 I H -0.8695 Soluble
207 C H 0.0000 Buried
208 N H -1.4838 Soluble
209 V H 0.0000 Buried
210 N H -1.9301 Soluble
211 H H 0.0000 Buried
212 K H -2.7782 Soluble
213 P H -1.6710 Soluble
214 S H -1.8575 Soluble
215 N H -2.5345 Soluble
216 T H -2.2229 Soluble
217 K H -2.6878 Soluble
218 V H -1.5145 Soluble
219 D H -2.2195 Soluble
220 K H -1.9468 Soluble
221 K H -1.8422 Soluble
222 V H 0.0000 Buried
223 E H -2.5652 Soluble
224 P H -1.9220 Soluble
225 K H -2.2828 Soluble
1 D L -3.3396 Soluble
2 I L 0.0000 Buried
3 E L -2.5970 Soluble
4 L L 0.0000 Buried
5 T L -0.6995 Soluble
6 Q L 0.0000 Buried
7 P L -0.5979 Soluble
8 P L -1.0067 Soluble
9 S L -1.0613 Soluble
11 V L -0.8139 Soluble
12 S L -0.5128 Soluble
13 V L 0.0000 Buried
14 A L -0.5811 Soluble
15 P L -1.2016 Soluble
16 G L -1.5234 Soluble
17 Q L -1.9528 Soluble
18 T L -1.4299 Soluble
19 A L 0.0000 Buried
20 R L -2.0495 Soluble
21 I L 0.0000 Buried
22 S L -0.8797 Soluble
23 C L 0.0000 Buried
24 S L -1.4316 Soluble
25 G L -2.2019 Soluble
26 D L -3.0726 Soluble
27 S L -2.3454 Soluble
28 I L 0.0000 Buried
29 G L -2.4081 Soluble
36 K L -2.6808 Soluble
37 K L -1.5177 Soluble
38 Y L -0.6882 Soluble
39 A L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.1540 Soluble
44 Q L 0.0000 Buried
45 K L -1.5002 Soluble
46 P L -1.2559 Soluble
47 G L -1.4140 Soluble
48 Q L -1.5795 Soluble
49 A L 0.0000 Buried
50 P L 0.0000 Buried
51 V L 1.5480 Aggregation-prone
52 L L 0.0000 Buried
53 V L 0.8285 Aggregation-prone
54 I L -0.0720 Soluble
55 Y L -0.6859 Soluble
56 K L -2.5197 Soluble
65 K L -3.2463 Soluble
67 R L -3.1363 Soluble
68 P L -1.9242 Soluble
69 S L -0.8855 Soluble
70 G L -0.1511 Soluble
71 I L 1.0224 Aggregation-prone
72 P L -0.4992 Soluble
74 E L -1.7480 Soluble
75 R L -1.2688 Soluble
76 F L -0.7474 Soluble
77 S L -0.8642 Soluble
78 G L -0.9735 Soluble
79 S L -0.9890 Soluble
80 N L -2.0149 Soluble
83 S L -1.7307 Soluble
84 G L -2.2113 Soluble
85 N L -2.6218 Soluble
86 T L -1.4945 Soluble
87 A L 0.0000 Buried
88 T L 0.0000 Buried
89 L L 0.0000 Buried
90 T L -1.2380 Soluble
91 I L 0.0000 Buried
92 S L -1.5532 Soluble
93 G L -1.5434 Soluble
94 T L 0.0000 Buried
95 Q L -1.9530 Soluble
96 A L -1.2955 Soluble
97 E L -1.9019 Soluble
98 D L 0.0000 Buried
99 E L -1.4352 Soluble
100 A L 0.0000 Buried
101 D L -1.8713 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 S L 0.0000 Buried
106 A L 0.0000 Buried
107 W L -0.6247 Soluble
108 G L -2.3953 Soluble
109 D L -3.1472 Soluble
114 K L -2.7885 Soluble
115 G L 0.0000 Buried
116 M L 0.0000 Buried
117 V L 0.0000 Buried
118 F L -0.4160 Soluble
119 G L 0.0000 Buried
120 G L -1.1242 Soluble
121 G L -1.2619 Soluble
122 T L 0.0000 Buried
123 K L -2.2725 Soluble
124 L L 0.0000 Buried
125 T L -0.6324 Soluble
126 V L 0.0000 Buried
127 L L 0.7549 Aggregation-prone
128 G L -0.3185 Soluble
129 Q L -0.7736 Soluble
130 P L -1.1516 Soluble
131 K L -2.1822 Soluble
132 A L -1.3092 Soluble
133 A L -0.8160 Soluble
134 P L -0.4844 Soluble
135 S L -0.6866 Soluble
136 V L 0.0000 Buried
137 T L -0.5401 Soluble
138 L L 0.0000 Buried
139 F L 0.0000 Buried
140 P L -0.1157 Soluble
141 P L -0.4221 Soluble
142 S L -1.0420 Soluble
143 S L -1.5343 Soluble
144 E L -2.1934 Soluble
145 E L 0.0000 Buried
146 L L -1.8272 Soluble
147 Q L -2.2344 Soluble
148 A L -1.7296 Soluble
149 N L -2.3855 Soluble
150 K L -2.0707 Soluble
151 A L 0.0000 Buried
152 T L 0.0000 Buried
153 L L 0.0000 Buried
154 V L 0.0000 Buried
155 C L 0.0000 Buried
156 L L 0.0000 Buried
157 I L 0.0000 Buried
158 S L -0.6010 Soluble
159 D L -1.1844 Soluble
160 F L 0.0000 Buried
161 Y L -1.1508 Soluble
162 P L -0.8800 Soluble
163 G L -0.4522 Soluble
164 A L -0.1624 Soluble
165 V L -0.0656 Soluble
166 T L -0.1318 Soluble
167 V L -0.0062 Soluble
168 A L -0.4807 Soluble
169 W L 0.0000 Buried
170 K L -0.9581 Soluble
171 A L 0.0000 Buried
172 D L -1.2842 Soluble
173 S L -0.8936 Soluble
174 S L -0.7816 Soluble
175 P L -0.8882 Soluble
176 V L -0.8504 Soluble
177 K L -1.6986 Soluble
178 A L -0.8386 Soluble
179 G L -0.6496 Soluble
180 V L -0.3462 Soluble
181 E L -0.3480 Soluble
182 T L 0.0184 Aggregation-prone
183 T L 0.0141 Aggregation-prone
184 T L -0.0867 Soluble
185 P L -0.2050 Soluble
186 S L -0.5065 Soluble
187 K L -1.1343 Soluble
188 Q L -0.9966 Soluble
189 S L -1.1118 Soluble
190 N L -1.2961 Soluble
191 N L -1.3471 Soluble
192 K L -1.4106 Soluble
193 Y L -0.6342 Soluble
194 A L 0.0000 Buried
195 A L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 Y L 0.0000 Buried
199 L L 0.0000 Buried
200 S L -0.4326 Soluble
201 L L 0.0000 Buried
202 T L -1.6808 Soluble
203 P L -2.3376 Soluble
204 E L -3.1376 Soluble
205 Q L -2.1891 Soluble
206 W L 0.0000 Buried
207 K L -3.2110 Soluble
208 S L -2.4400 Soluble
209 H L -2.4147 Soluble
210 R L -2.5399 Soluble
211 S L -1.5255 Soluble
212 Y L 0.0000 Buried
213 S L 0.0000 Buried
214 C L 0.0000 Buried
215 Q L -1.2063 Soluble
216 V L 0.0000 Buried
217 T L -0.7602 Soluble
218 H L 0.0000 Buried
219 E L -2.2697 Soluble
220 G L -1.4413 Soluble
221 S L -0.9610 Soluble
222 T L -0.8500 Soluble
223 V L -0.9315 Soluble
224 E L -2.3116 Soluble
225 K L -1.9618 Soluble
226 T L -1.0329 Soluble
227 V L -0.3706 Soluble
228 A L -0.8454 Soluble
229 P L -1.0090 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.622 4.4468 CSV PDB
4.5 -0.6548 4.4326 CSV PDB
5.0 -0.6933 4.4164 CSV PDB
5.5 -0.7282 4.3993 CSV PDB
6.0 -0.7482 4.3825 CSV PDB
6.5 -0.7436 4.367 CSV PDB
7.0 -0.7143 4.3536 CSV PDB
7.5 -0.6689 4.3424 CSV PDB
8.0 -0.6153 4.3333 CSV PDB
8.5 -0.5565 4.3271 CSV PDB
9.0 -0.4936 4.3239 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH