Gremubamab / 6cyf

A4DAB136_6cyf_M_L | Source PDB: 6cyf | Chains: M, L | Done
98.7
Yes
10 Å
Yes
Avg A4D Score
-0.662
Max Score
1.8361
Min Score
-3.6207
Total Score
-288.61
Score CDR
-0.1656
Score non-CDR
-0.7277

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYAMDWVRQAPGKGLEWVSAITMSGITAYYTDDVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKEEFLPGTHYFYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKAIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKLLIYSASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCLQDYNYPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) M (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6207
Maximum Score 1.8361
Average Score -0.662
Total Score -288.6107

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E M -1.8377 Soluble
2 V M -0.7171 Soluble
3 Q M -0.7627 Soluble
4 L M 0.0000 Buried
5 V M 1.1289 Aggregation-prone
6 E M 0.1962 Aggregation-prone
7 S M -0.5206 Soluble
8 G M -0.9784 Soluble
9 G M -0.5529 Soluble
11 G M -0.0395 Soluble
12 L M 0.5005 Aggregation-prone
13 V M -0.3946 Soluble
14 Q M -1.5835 Soluble
15 P M -1.9447 Soluble
16 G M -1.5077 Soluble
17 G M -1.0723 Soluble
18 S M -1.1149 Soluble
19 L M -0.8798 Soluble
20 R M -1.8962 Soluble
21 L M 0.0000 Buried
22 S M -0.4017 Soluble
23 C M 0.0000 Buried
24 A M -0.0230 Soluble
25 A M 0.0000 Buried
26 S M -0.5543 Soluble
27 G M -0.9336 Soluble
28 F M -0.3102 Soluble
29 T M -0.1200 Soluble
30 F M 0.0000 Buried
35 S M -0.6308 Soluble
36 S M -0.2059 Soluble
37 Y M 0.1137 Aggregation-prone
38 A M 0.0125 Aggregation-prone
39 M M 0.0000 Buried
40 D M 0.0000 Buried
41 W M 0.0000 Buried
42 V M 0.0000 Buried
43 R M 0.0000 Buried
44 Q M -0.7873 Soluble
45 A M -1.1798 Soluble
46 P M -0.9708 Soluble
47 G M -1.5088 Soluble
48 K M -2.3727 Soluble
49 G M -1.6366 Soluble
50 L M 0.0000 Buried
51 E M -1.1481 Soluble
52 W M 0.0000 Buried
53 V M 0.0000 Buried
54 S M 0.0000 Buried
55 A M 0.0000 Buried
56 I M 0.0000 Buried
57 T M 0.3454 Aggregation-prone
58 M M 0.1559 Aggregation-prone
59 S M 0.2413 Aggregation-prone
62 G M 0.4707 Aggregation-prone
63 I M 1.8361 Aggregation-prone
64 T M 0.9408 Aggregation-prone
65 A M 0.6025 Aggregation-prone
66 Y M 0.0649 Aggregation-prone
67 Y M -0.7522 Soluble
68 T M 0.0000 Buried
69 D M -2.7649 Soluble
70 D M -2.6575 Soluble
71 V M 0.0000 Buried
72 K M -2.7588 Soluble
74 G M -1.8565 Soluble
75 R M -1.4703 Soluble
76 F M 0.0000 Buried
77 T M -0.7576 Soluble
78 I M 0.0000 Buried
79 S M -0.1205 Soluble
80 R M -0.7565 Soluble
81 D M -1.6475 Soluble
82 N M -1.6790 Soluble
83 S M -1.5827 Soluble
84 K M -2.3816 Soluble
85 N M -1.7117 Soluble
86 T M -1.0046 Soluble
87 L M 0.0000 Buried
88 Y M -0.5774 Soluble
89 L M 0.0000 Buried
90 Q M -1.1013 Soluble
91 M M 0.0000 Buried
92 N M -1.4750 Soluble
93 S M -1.4051 Soluble
94 L M 0.0000 Buried
95 R M -2.7302 Soluble
96 A M -1.9215 Soluble
97 E M -2.4485 Soluble
98 D M 0.0000 Buried
99 T M -0.7665 Soluble
100 A M 0.0000 Buried
101 V M 0.1974 Aggregation-prone
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 A M 0.0000 Buried
106 K M 0.0000 Buried
107 E M 0.0000 Buried
108 E M -0.0139 Soluble
109 F M 0.3009 Aggregation-prone
110 L M 0.3810 Aggregation-prone
111 P M -0.3088 Soluble
111A G M -0.5639 Soluble
111B T M -0.3490 Soluble
112B H M -0.4974 Soluble
112A Y M 0.5175 Aggregation-prone
112 F M 0.8892 Aggregation-prone
113 Y M 0.4926 Aggregation-prone
114 G M 0.0000 Buried
115 M M 0.0000 Buried
116 D M -0.7020 Soluble
117 V M -0.2261 Soluble
118 W M -0.4027 Soluble
119 G M 0.0000 Buried
120 Q M -1.2720 Soluble
121 G M -0.5761 Soluble
122 T M -0.2145 Soluble
123 T M -0.0889 Soluble
124 V M 0.0000 Buried
125 T M 0.0000 Buried
126 V M 0.0000 Buried
127 S M -0.9674 Soluble
128 S M -0.7702 Soluble
129 A M -0.4598 Soluble
130 S M -0.5694 Soluble
131 T M -0.6495 Soluble
132 K M -1.3247 Soluble
133 G M -1.4463 Soluble
134 P M -0.6491 Soluble
135 S M -0.3934 Soluble
136 V M 0.0000 Buried
137 F M 0.0000 Buried
138 P M -1.0373 Soluble
139 L M 0.0000 Buried
140 A M -1.1071 Soluble
141 P M 0.0000 Buried
142 S M -0.9592 Soluble
143 S M -1.2054 Soluble
144 K M -1.9058 Soluble
145 G M -0.8290 Soluble
146 G M -1.0409 Soluble
147 T M -0.7706 Soluble
148 A M 0.0000 Buried
149 A M 0.0000 Buried
150 L M 0.0000 Buried
151 G M 0.0000 Buried
152 C M 0.0000 Buried
153 L M 0.0000 Buried
154 V M 0.0000 Buried
155 K M 0.0000 Buried
156 D M -0.3893 Soluble
157 Y M 0.0000 Buried
158 F M 0.0000 Buried
159 P M 0.0000 Buried
160 E M -0.6417 Soluble
161 P M -0.8614 Soluble
162 V M -0.7719 Soluble
163 T M -0.6748 Soluble
164 V M -0.3792 Soluble
165 S M -0.3500 Soluble
166 W M 0.0000 Buried
167 N M -0.6197 Soluble
168 S M -0.5630 Soluble
169 G M -0.4626 Soluble
170 A M -0.1926 Soluble
171 L M 0.0372 Aggregation-prone
172 T M -0.1420 Soluble
173 S M -0.1592 Soluble
174 G M -0.1498 Soluble
175 V M 0.1797 Aggregation-prone
176 H M -0.2555 Soluble
177 T M 0.0364 Aggregation-prone
178 F M 0.0000 Buried
179 P M -0.3443 Soluble
180 A M 0.2369 Aggregation-prone
181 V M 0.6463 Aggregation-prone
182 L M 1.3111 Aggregation-prone
183 Q M 0.4297 Aggregation-prone
184 S M 0.0089 Aggregation-prone
185 S M -0.1667 Soluble
186 G M 0.0919 Aggregation-prone
187 L M 0.1002 Aggregation-prone
188 Y M 0.4345 Aggregation-prone
189 S M 0.0000 Buried
190 L M 0.0000 Buried
191 S M 0.0000 Buried
192 S M 0.0000 Buried
193 V M 0.0000 Buried
194 V M 0.0000 Buried
195 T M -0.1758 Soluble
196 V M 0.0000 Buried
197 P M -0.5669 Soluble
198 S M -0.8165 Soluble
199 S M -0.7107 Soluble
200 S M -0.6482 Soluble
201 L M -0.7772 Soluble
202 G M -0.9800 Soluble
203 T M -0.7011 Soluble
204 Q M -1.1511 Soluble
205 T M -1.0563 Soluble
206 Y M 0.0000 Buried
207 I M -1.0238 Soluble
208 C M 0.0000 Buried
209 N M -1.4114 Soluble
210 V M 0.0000 Buried
211 N M -2.1608 Soluble
212 H M 0.0000 Buried
213 K M -2.7967 Soluble
214 P M -1.6979 Soluble
215 S M -1.9085 Soluble
216 N M -2.6104 Soluble
217 T M -2.1150 Soluble
218 K M -2.7294 Soluble
219 V M -1.4350 Soluble
220 D M -2.2939 Soluble
221 K M -1.8141 Soluble
222 K M -2.3985 Soluble
223 V M 0.0000 Buried
224 E M -2.7913 Soluble
225 P M -2.0867 Soluble
226 K M -2.0821 Soluble
1 A L -0.7361 Soluble
2 I L 0.0000 Buried
3 Q L -1.8734 Soluble
4 M L 0.0000 Buried
5 T L -1.4711 Soluble
6 Q L 0.0000 Buried
7 S L -0.8571 Soluble
8 P L -0.4545 Soluble
9 S L -0.7749 Soluble
10 S L -0.5683 Soluble
11 L L -0.3644 Soluble
12 S L -0.7460 Soluble
13 A L 0.0000 Buried
14 S L -0.5721 Soluble
15 V L 0.2610 Aggregation-prone
16 G L -0.6881 Soluble
17 D L -1.6824 Soluble
18 R L -2.2947 Soluble
19 V L 0.0000 Buried
20 T L -0.6152 Soluble
21 I L 0.0000 Buried
22 T L -0.8295 Soluble
23 C L 0.0000 Buried
24 R L -2.7996 Soluble
25 A L -2.1331 Soluble
26 S L -1.8234 Soluble
27 Q L -2.3084 Soluble
28 G L -2.0890 Soluble
29 I L 0.0000 Buried
36 R L -2.6174 Soluble
37 N L -1.9137 Soluble
38 D L 0.0000 Buried
39 L L 0.0000 Buried
40 G L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.4863 Soluble
46 P L -1.4077 Soluble
47 G L -1.5419 Soluble
48 K L -2.2650 Soluble
49 A L -1.5080 Soluble
50 P L 0.0000 Buried
51 K L -1.5938 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0456 Aggregation-prone
56 S L -0.4337 Soluble
57 A L 0.0000 Buried
65 S L -0.4045 Soluble
66 T L 0.0200 Aggregation-prone
67 L L 0.1921 Aggregation-prone
68 Q L -0.3598 Soluble
69 S L -0.3832 Soluble
70 G L -0.5092 Soluble
71 V L -0.3525 Soluble
72 P L -0.3552 Soluble
74 S L -0.4332 Soluble
75 R L -0.7838 Soluble
76 F L 0.0000 Buried
77 S L -0.3010 Soluble
78 G L -0.2520 Soluble
79 S L -0.6798 Soluble
80 G L -1.4258 Soluble
83 S L -1.6585 Soluble
84 G L -2.1575 Soluble
85 T L -2.2643 Soluble
86 D L -2.4582 Soluble
87 F L 0.0000 Buried
88 T L -0.7062 Soluble
89 L L 0.0000 Buried
90 T L -0.5751 Soluble
91 I L 0.0000 Buried
92 S L -1.2918 Soluble
93 S L -1.0647 Soluble
94 L L 0.0000 Buried
95 Q L -0.7729 Soluble
96 P L -0.8243 Soluble
97 E L -1.8374 Soluble
98 D L 0.0000 Buried
99 F L -0.3277 Soluble
100 A L 0.0000 Buried
101 T L -0.6900 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 D L 0.0000 Buried
108 Y L -0.1053 Soluble
109 N L -0.3722 Soluble
114 Y L 0.0613 Aggregation-prone
115 P L -0.1645 Soluble
116 W L 0.0000 Buried
117 T L -0.5386 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.7373 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.0623 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.4289 Soluble
127 K L -1.4736 Soluble
128 R L -1.2101 Soluble
129 T L -0.3224 Soluble
130 V L 0.1734 Aggregation-prone
131 A L -0.0028 Soluble
132 A L -0.1755 Soluble
133 P L 0.0000 Buried
134 S L -0.1598 Soluble
135 V L -0.0914 Soluble
136 F L 0.0000 Buried
137 I L -0.0548 Soluble
138 F L 0.0000 Buried
139 P L -0.5211 Soluble
140 P L 0.0000 Buried
141 S L -1.4269 Soluble
142 D L -2.6479 Soluble
143 E L -2.4532 Soluble
144 Q L 0.0000 Buried
145 L L -2.0166 Soluble
146 K L -2.5773 Soluble
147 S L -1.5964 Soluble
148 G L -1.1356 Soluble
149 T L -0.7591 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6574 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3622 Soluble
162 R L -1.7906 Soluble
163 E L -2.6407 Soluble
164 A L -1.9836 Soluble
165 K L -2.1362 Soluble
166 V L -1.0538 Soluble
167 Q L -0.4520 Soluble
168 W L 0.0000 Buried
169 K L -0.5486 Soluble
170 V L 0.0000 Buried
171 D L -1.9606 Soluble
172 N L -1.4189 Soluble
173 A L -0.3205 Soluble
174 L L 0.7008 Aggregation-prone
175 Q L -0.3952 Soluble
176 S L -0.6626 Soluble
177 G L -1.3074 Soluble
178 N L -1.6847 Soluble
179 S L -1.4456 Soluble
180 Q L -1.2715 Soluble
181 E L -1.7651 Soluble
182 S L -0.6558 Soluble
183 V L -0.4499 Soluble
184 T L -0.9423 Soluble
185 E L -2.0798 Soluble
186 Q L -1.6260 Soluble
187 D L -1.8466 Soluble
188 S L -1.6137 Soluble
189 K L -2.1721 Soluble
190 D L -1.5253 Soluble
191 S L -1.6165 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6715 Soluble
199 L L 0.0000 Buried
200 T L -0.6105 Soluble
201 L L -0.7882 Soluble
202 S L -1.1365 Soluble
203 K L -1.9677 Soluble
204 A L -1.7988 Soluble
205 D L -2.3844 Soluble
206 Y L 0.0000 Buried
207 E L -3.6207 Soluble
208 K L -3.5912 Soluble
209 H L -3.1204 Soluble
210 K L -3.2560 Soluble
211 V L -1.4483 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7321 Soluble
216 V L 0.0000 Buried
217 T L -1.1920 Soluble
218 H L 0.0000 Buried
219 Q L -1.7105 Soluble
220 G L -0.5913 Soluble
221 L L -0.3321 Soluble
222 S L -0.5120 Soluble
223 S L -0.4506 Soluble
224 P L -0.5649 Soluble
225 V L -0.0275 Soluble
226 T L -0.5297 Soluble
227 K L -0.8414 Soluble
228 S L -0.6338 Soluble
229 F L -0.9125 Soluble
230 N L -1.8947 Soluble
231 R L -2.5644 Soluble
232 G L -2.0784 Soluble
233 E L -2.1900 Soluble
234 C L -0.8004 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6956 2.557 CSV PDB
4.5 -0.7344 2.557 CSV PDB
5.0 -0.7791 2.557 CSV PDB
5.5 -0.8204 2.557 CSV PDB
6.0 -0.8471 2.557 CSV PDB
6.5 -0.8507 2.557 CSV PDB
7.0 -0.8318 2.557 CSV PDB
7.5 -0.7985 2.557 CSV PDB
8.0 -0.7575 2.557 CSV PDB
8.5 -0.7107 2.557 CSV PDB
9.0 -0.6584 2.557 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH