Lampalizumab / 4d9q

A4DAB150_4d9q_E_D | Source PDB: 4d9q | Chains: E, D | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.703
Max Score
1.1385
Min Score
-3.5028
Total Score
-298.78
Score CDR
-0.6873
Score non-CDR
-0.7047

Calculation Parameters

Chain Sequence(s) EVQLVQSGPELKKPGASVKVSCKASGYTFTNYGMNWVRQAPGQGLEWMGWINTYTGETTYADDFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCEREGGVNNWGQGTLVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIQVTQSPSSLSASVGDRVTITCITSTDIDDDMNWYQQKPGKVPKLLISGGNTLRPGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCLQSDSLPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) E (Heavy), D (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5028
Maximum Score 1.1385
Average Score -0.703
Total Score -298.7837

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E E -2.2757 Soluble
2 V E 0.0000 Buried
3 Q E -1.9787 Soluble
4 L E 0.0000 Buried
5 V E -0.2755 Soluble
6 Q E 0.0000 Buried
7 S E -0.5862 Soluble
8 G E -0.6818 Soluble
9 P E -0.7036 Soluble
11 E E -1.0572 Soluble
12 L E -0.7337 Soluble
13 K E -1.6439 Soluble
14 K E -2.4293 Soluble
15 P E -2.0995 Soluble
16 G E -1.4903 Soluble
17 A E -1.2040 Soluble
18 S E -1.2517 Soluble
19 V E 0.0000 Buried
20 K E -1.7678 Soluble
21 V E 0.0000 Buried
22 S E -0.7470 Soluble
23 C E 0.0000 Buried
24 K E -1.2649 Soluble
25 A E 0.0000 Buried
26 S E -1.0801 Soluble
27 G E -1.4247 Soluble
28 Y E -0.7767 Soluble
29 T E -0.4548 Soluble
30 F E 0.0000 Buried
35 T E -0.1230 Soluble
36 N E -0.9492 Soluble
37 Y E -0.3760 Soluble
38 G E 0.0000 Buried
39 M E 0.0000 Buried
40 N E 0.0000 Buried
41 W E 0.0000 Buried
42 V E 0.0000 Buried
43 R E -0.5204 Soluble
44 Q E -0.7108 Soluble
45 A E -1.0144 Soluble
46 P E -1.0257 Soluble
47 G E -1.2483 Soluble
48 Q E -1.8495 Soluble
49 G E -1.3653 Soluble
50 L E 0.0000 Buried
51 E E -1.1686 Soluble
52 W E 0.0000 Buried
53 M E 0.0000 Buried
54 G E 0.0000 Buried
55 W E -0.0594 Soluble
56 I E 0.0000 Buried
57 N E -0.6358 Soluble
58 T E 0.0000 Buried
59 Y E 0.6430 Aggregation-prone
62 T E -0.1990 Soluble
63 G E -0.9072 Soluble
64 E E -1.8444 Soluble
65 T E -0.7557 Soluble
66 T E -0.4280 Soluble
67 Y E -0.7616 Soluble
68 A E -1.9228 Soluble
69 D E -3.5028 Soluble
70 D E -3.2786 Soluble
71 F E 0.0000 Buried
72 K E -2.9125 Soluble
74 G E -1.8324 Soluble
75 R E -1.2398 Soluble
76 F E 0.0000 Buried
77 V E 0.1528 Aggregation-prone
78 F E 0.0000 Buried
79 S E -0.3545 Soluble
80 L E 0.2332 Aggregation-prone
81 D E -0.3607 Soluble
82 T E 0.2756 Aggregation-prone
83 S E 0.4141 Aggregation-prone
84 V E 1.1385 Aggregation-prone
85 S E 0.1086 Aggregation-prone
86 T E 0.0000 Buried
87 A E 0.0000 Buried
88 Y E -0.4829 Soluble
89 L E 0.0000 Buried
90 Q E -0.8980 Soluble
91 I E 0.0000 Buried
92 S E -0.9127 Soluble
93 S E -1.1389 Soluble
94 L E 0.0000 Buried
95 K E -2.4388 Soluble
96 A E -1.8795 Soluble
97 E E -2.2786 Soluble
98 D E 0.0000 Buried
99 T E -0.8847 Soluble
100 A E 0.0000 Buried
101 V E 0.1778 Aggregation-prone
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 E E 0.0000 Buried
106 R E 0.0000 Buried
107 E E -0.4937 Soluble
108 G E -0.4058 Soluble
114 G E 0.1205 Aggregation-prone
115 V E 1.1365 Aggregation-prone
116 N E -0.0945 Soluble
117 N E -0.5368 Soluble
118 W E -0.5522 Soluble
119 G E 0.0000 Buried
120 Q E -1.1666 Soluble
121 G E -0.4664 Soluble
122 T E 0.0000 Buried
123 L E -0.0823 Soluble
124 V E 0.0000 Buried
125 T E 0.0000 Buried
126 V E 0.0000 Buried
127 S E -1.2628 Soluble
128 S E -0.9710 Soluble
129 A E -0.5956 Soluble
130 S E -0.6843 Soluble
131 T E -0.6116 Soluble
132 K E -1.1007 Soluble
133 G E -1.3563 Soluble
134 P E -0.6265 Soluble
135 S E -0.4563 Soluble
136 V E 0.0000 Buried
137 F E 0.0000 Buried
138 P E 0.0000 Buried
139 L E 0.0000 Buried
140 A E -1.2299 Soluble
141 P E 0.0000 Buried
142 S E -0.8335 Soluble
143 S E -0.3180 Soluble
144 G E -0.6432 Soluble
145 T E -0.4436 Soluble
146 A E 0.0000 Buried
147 A E 0.0000 Buried
148 L E 0.0000 Buried
149 G E 0.0000 Buried
150 C E 0.0000 Buried
151 L E 0.0000 Buried
152 V E 0.0000 Buried
153 K E 0.0000 Buried
154 D E -0.4446 Soluble
155 Y E 0.0000 Buried
156 F E 0.0000 Buried
157 P E 0.0000 Buried
158 E E -0.4782 Soluble
159 P E -0.6717 Soluble
160 V E -0.6387 Soluble
161 T E -0.6103 Soluble
162 V E -0.2408 Soluble
163 S E -0.4300 Soluble
164 W E 0.0000 Buried
165 N E -0.7197 Soluble
166 S E -0.6573 Soluble
167 G E -0.5221 Soluble
168 A E -0.2506 Soluble
169 L E -0.0113 Soluble
170 T E -0.1789 Soluble
171 S E -0.1918 Soluble
172 G E -0.2247 Soluble
173 V E 0.1570 Aggregation-prone
174 H E -0.2068 Soluble
175 T E -0.0172 Soluble
176 F E 0.0000 Buried
177 P E -0.3014 Soluble
178 A E 0.2109 Aggregation-prone
179 V E 0.4974 Aggregation-prone
180 L E 0.9039 Aggregation-prone
181 Q E 0.1661 Aggregation-prone
182 S E -0.1506 Soluble
183 S E -0.2489 Soluble
184 G E -0.0031 Soluble
185 L E 0.1285 Aggregation-prone
186 Y E 0.3980 Aggregation-prone
187 S E 0.0000 Buried
188 L E 0.0000 Buried
189 S E 0.0000 Buried
190 S E 0.0000 Buried
191 V E 0.0000 Buried
192 V E 0.0000 Buried
193 T E -0.1358 Soluble
194 V E 0.0000 Buried
195 P E -0.5576 Soluble
196 S E -0.5705 Soluble
197 S E -0.6084 Soluble
198 S E -0.5978 Soluble
199 L E -0.8241 Soluble
200 G E -0.9941 Soluble
201 T E -0.7094 Soluble
202 Q E -1.2494 Soluble
203 T E -1.0724 Soluble
204 Y E 0.0000 Buried
205 I E -1.1561 Soluble
206 C E 0.0000 Buried
207 N E -1.5410 Soluble
208 V E 0.0000 Buried
209 N E -2.1992 Soluble
210 H E 0.0000 Buried
211 K E -2.8787 Soluble
212 P E -1.6790 Soluble
213 S E -1.8332 Soluble
214 N E -2.6213 Soluble
215 T E -2.1525 Soluble
216 K E -2.8795 Soluble
217 V E -1.9018 Soluble
218 D E -2.5717 Soluble
219 K E -2.0932 Soluble
220 K E -2.4210 Soluble
221 V E 0.0000 Buried
222 E E -2.8681 Soluble
223 P E -2.0003 Soluble
224 K E -2.5631 Soluble
225 S E -1.4702 Soluble
1 D D -2.2132 Soluble
2 I D -1.5483 Soluble
3 Q D -1.4887 Soluble
4 V D 0.0000 Buried
5 T D -0.5514 Soluble
6 Q D 0.0000 Buried
7 S D -0.4062 Soluble
8 P D -0.5083 Soluble
9 S D -0.6879 Soluble
10 S D -0.5630 Soluble
11 L D -0.4572 Soluble
12 S D -1.0087 Soluble
13 A D -1.0223 Soluble
14 S D -0.7734 Soluble
15 V D 0.2957 Aggregation-prone
16 G D -0.6958 Soluble
17 D D -1.6679 Soluble
18 R D -2.3608 Soluble
19 V D 0.0000 Buried
20 T D -0.6496 Soluble
21 I D 0.0000 Buried
22 T D -0.3192 Soluble
23 C D 0.0000 Buried
24 I D -0.3725 Soluble
25 T D 0.0000 Buried
26 S D -1.2018 Soluble
27 T D -1.5508 Soluble
28 D D -2.8673 Soluble
29 I D 0.0000 Buried
36 D D -3.4390 Soluble
37 D D -2.9855 Soluble
38 D D -2.3152 Soluble
39 M D 0.0000 Buried
40 N D 0.0000 Buried
41 W D 0.0000 Buried
42 Y D 0.0000 Buried
43 Q D 0.0000 Buried
44 Q D 0.0000 Buried
45 K D -1.4309 Soluble
46 P D -1.2799 Soluble
47 G D -1.4660 Soluble
48 K D -2.1377 Soluble
49 V D -0.9882 Soluble
50 P D 0.0000 Buried
51 K D -1.5440 Soluble
52 L D 0.0000 Buried
53 L D 0.0000 Buried
54 I D 0.0000 Buried
55 S D 0.0000 Buried
56 G D -1.2949 Soluble
57 G D -1.3990 Soluble
65 N D -0.9452 Soluble
66 T D -0.3890 Soluble
67 L D 0.0058 Aggregation-prone
68 R D -0.1839 Soluble
69 P D -0.3011 Soluble
70 G D -0.5670 Soluble
71 V D -0.3910 Soluble
72 P D -0.3586 Soluble
74 S D -0.4487 Soluble
75 R D -0.7383 Soluble
76 F D 0.0000 Buried
77 S D -0.3627 Soluble
78 G D -0.4385 Soluble
79 S D -0.9380 Soluble
80 G D -1.3763 Soluble
83 S D -2.2602 Soluble
84 G D -2.3798 Soluble
85 T D -1.9456 Soluble
86 D D -2.2054 Soluble
87 F D 0.0000 Buried
88 T D -0.5622 Soluble
89 L D 0.0000 Buried
90 T D -0.6652 Soluble
91 I D 0.0000 Buried
92 S D -1.5038 Soluble
93 S D -1.1639 Soluble
94 L D 0.0000 Buried
95 Q D -0.9438 Soluble
96 P D -1.3297 Soluble
97 E D -1.4995 Soluble
98 D D 0.0000 Buried
99 V D 0.0000 Buried
100 A D 0.0000 Buried
101 T D -0.6456 Soluble
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 L D 0.0000 Buried
106 Q D 0.0000 Buried
107 S D -0.7911 Soluble
108 D D -1.6536 Soluble
109 S D -0.5209 Soluble
114 L D 0.2511 Aggregation-prone
115 P D -0.3108 Soluble
116 Y D -0.2602 Soluble
117 T D -0.7083 Soluble
118 F D 0.0000 Buried
119 G D 0.0000 Buried
120 Q D -1.6007 Soluble
121 G D 0.0000 Buried
122 T D 0.0000 Buried
123 K D -0.9981 Soluble
124 V D 0.0000 Buried
125 E D -1.0703 Soluble
126 I D 0.0000 Buried
127 K D -1.4148 Soluble
128 R D -0.8297 Soluble
129 T D -0.1411 Soluble
130 V D 0.2502 Aggregation-prone
131 A D 0.0821 Aggregation-prone
132 A D -0.1576 Soluble
133 P D 0.0000 Buried
134 S D -0.1469 Soluble
135 V D 0.0000 Buried
136 F D 0.1062 Aggregation-prone
137 I D 0.1046 Aggregation-prone
138 F D 0.0000 Buried
139 P D -0.5638 Soluble
140 P D -0.8695 Soluble
141 S D -1.8984 Soluble
142 D D -3.2219 Soluble
143 E D -3.2136 Soluble
144 Q D 0.0000 Buried
145 L D -2.3344 Soluble
146 K D -2.9228 Soluble
147 S D -1.8124 Soluble
148 G D -1.2986 Soluble
149 T D -1.0408 Soluble
150 A D 0.0000 Buried
151 S D 0.0000 Buried
152 V D 0.0000 Buried
153 V D 0.0000 Buried
154 C D 0.0000 Buried
155 L D 0.0000 Buried
156 L D 0.0000 Buried
157 N D 0.0000 Buried
158 N D -0.8599 Soluble
159 F D 0.0000 Buried
160 Y D 0.0000 Buried
161 P D -1.7514 Soluble
162 R D -2.8984 Soluble
163 E D -3.2232 Soluble
164 A D -2.3489 Soluble
165 K D -2.5681 Soluble
166 V D -1.0249 Soluble
167 Q D -0.4492 Soluble
168 W D 0.0000 Buried
169 K D -0.5264 Soluble
170 V D 0.0000 Buried
171 D D -1.9147 Soluble
172 N D -1.5763 Soluble
173 A D -0.2690 Soluble
174 L D 0.7848 Aggregation-prone
175 Q D 0.0135 Aggregation-prone
176 S D -0.3415 Soluble
177 G D -0.8375 Soluble
178 N D -0.7325 Soluble
179 S D -0.9134 Soluble
180 Q D -1.0796 Soluble
181 E D -1.6009 Soluble
182 S D -0.6885 Soluble
183 V D -0.5620 Soluble
184 T D -0.6951 Soluble
185 E D -1.2989 Soluble
186 Q D 0.0000 Buried
187 D D -1.5799 Soluble
188 S D -1.8481 Soluble
189 K D -2.3981 Soluble
190 D D -1.6583 Soluble
191 S D 0.0000 Buried
192 T D 0.0000 Buried
193 Y D 0.0000 Buried
194 S D 0.0000 Buried
195 L D 0.0000 Buried
196 S D 0.0000 Buried
197 S D 0.0000 Buried
198 T D -0.5151 Soluble
199 L D 0.0000 Buried
200 T D -0.3772 Soluble
201 L D -0.5638 Soluble
202 S D -0.9553 Soluble
203 K D -1.9490 Soluble
204 A D -1.6663 Soluble
205 D D -2.2057 Soluble
206 Y D 0.0000 Buried
207 E D -2.9895 Soluble
208 K D -3.2418 Soluble
209 H D -2.8817 Soluble
210 K D -3.3000 Soluble
211 V D -1.6087 Soluble
212 Y D 0.0000 Buried
213 A D 0.0000 Buried
214 C D 0.0000 Buried
215 E D -0.6869 Soluble
216 V D 0.0000 Buried
217 T D -1.2236 Soluble
218 H D 0.0000 Buried
219 Q D -1.7576 Soluble
220 G D -0.4565 Soluble
221 L D -0.2059 Soluble
222 S D -0.4391 Soluble
223 S D -0.3954 Soluble
224 P D -0.5231 Soluble
225 V D 0.0896 Aggregation-prone
226 T D -0.3870 Soluble
227 K D -0.5918 Soluble
228 S D -0.5856 Soluble
229 F D -1.0198 Soluble
230 N D -2.1483 Soluble
231 R D -2.5747 Soluble
232 G D -2.1768 Soluble
233 E D -2.5736 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6535 1.8426 CSV PDB
4.5 -0.7061 1.8093 CSV PDB
5.0 -0.7658 1.7991 CSV PDB
5.5 -0.8217 1.7991 CSV PDB
6.0 -0.8623 1.7991 CSV PDB
6.5 -0.8791 1.7991 CSV PDB
7.0 -0.8721 1.7991 CSV PDB
7.5 -0.8496 1.7991 CSV PDB
8.0 -0.8184 1.7991 CSV PDB
8.5 -0.7797 1.7991 CSV PDB
9.0 -0.7322 1.7991 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH