Matuzumab / 5vr9

A4DAB167_5vr9_H_L | Source PDB: 5vr9 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7337
Max Score
0.8369
Min Score
-3.612
Total Score
-319.91
Score CDR
-0.5332
Score non-CDR
-0.758

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGASVKVSCKASGYTFTSHWMHWVRQAPGQGLEWIGEFNPSNGRTNYNEKFKSKATMTVDTSTNTAYMELSSLRSEDTAVYYCASRDYDYDGRYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCSASSSVTYMYWYQQKPGKAPKLLIYDTSNLASGVPSRFSGSGSGTDYTFTISSLQPEDIATYYCQQWSSHIFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.612
Maximum Score 0.8369
Average Score -0.7337
Total Score -319.9129

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.0485 Soluble
2 V H 0.0496 Aggregation-prone
3 Q H -0.4384 Soluble
4 L H 0.0000 Buried
5 V H 0.3713 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.6433 Soluble
8 G H -1.0973 Soluble
9 A H 0.0000 Buried
11 E H -1.4969 Soluble
12 V H -1.0272 Soluble
13 K H -1.7654 Soluble
14 K H -2.4102 Soluble
15 P H -1.8614 Soluble
16 G H -1.5159 Soluble
17 A H -1.2133 Soluble
18 S H -1.3139 Soluble
19 V H 0.0000 Buried
20 K H -1.9189 Soluble
21 V H 0.0000 Buried
22 S H -0.4833 Soluble
23 C H 0.0000 Buried
24 K H -0.8058 Soluble
25 A H -0.4023 Soluble
26 S H -0.4474 Soluble
27 G H -0.5366 Soluble
28 Y H 0.0511 Aggregation-prone
29 T H -0.2198 Soluble
30 F H 0.0000 Buried
35 T H -0.3455 Soluble
36 S H -0.4745 Soluble
37 H H 0.0000 Buried
38 W H -0.2536 Soluble
39 M H 0.0000 Buried
40 H H -0.0540 Soluble
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.5803 Soluble
44 Q H -0.7550 Soluble
45 A H -1.0771 Soluble
46 P H -1.0258 Soluble
47 G H -1.2263 Soluble
48 Q H -1.9061 Soluble
49 G H -1.4453 Soluble
50 L H 0.0000 Buried
51 E H -1.0575 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 E H 0.0000 Buried
56 F H 0.0000 Buried
57 N H -1.2327 Soluble
58 P H 0.0000 Buried
59 S H -1.3355 Soluble
62 N H -2.1828 Soluble
63 G H -1.9178 Soluble
64 R H -2.4367 Soluble
65 T H -1.2165 Soluble
66 N H -1.0539 Soluble
67 Y H -1.1789 Soluble
68 N H 0.0000 Buried
69 E H -3.1765 Soluble
70 K H -3.1135 Soluble
71 F H 0.0000 Buried
72 K H -2.9597 Soluble
74 S H -1.8231 Soluble
75 K H -1.5570 Soluble
76 A H 0.0000 Buried
77 T H -0.8375 Soluble
78 M H 0.0000 Buried
79 T H -0.7950 Soluble
80 V H -0.9326 Soluble
81 D H -1.0578 Soluble
82 T H -0.9544 Soluble
83 S H -0.6006 Soluble
84 T H -0.6754 Soluble
85 N H -0.8938 Soluble
86 T H -0.6257 Soluble
87 A H 0.0000 Buried
88 Y H -0.4331 Soluble
89 M H 0.0000 Buried
90 E H -1.3540 Soluble
91 L H 0.0000 Buried
92 S H -1.0618 Soluble
93 S H -1.1435 Soluble
94 L H 0.0000 Buried
95 R H -2.6790 Soluble
96 S H -2.0693 Soluble
97 E H -2.4094 Soluble
98 D H 0.0000 Buried
99 T H -0.9307 Soluble
100 A H 0.0000 Buried
101 V H 0.1911 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H 0.0000 Buried
107 R H -0.3170 Soluble
108 D H -0.4336 Soluble
109 Y H -0.0550 Soluble
110 D H -0.5200 Soluble
111 Y H 0.0692 Aggregation-prone
112A D H -1.7706 Soluble
112 G H -1.5420 Soluble
113 R H -2.0936 Soluble
114 Y H -0.7296 Soluble
115 F H 0.0000 Buried
116 D H -0.4427 Soluble
117 Y H -0.1654 Soluble
118 W H -0.4151 Soluble
119 G H 0.0000 Buried
120 Q H -1.2665 Soluble
121 G H -0.5810 Soluble
122 T H 0.0000 Buried
123 L H -0.2066 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.1832 Soluble
128 S H -0.9556 Soluble
129 A H -0.6666 Soluble
130 S H -0.8338 Soluble
131 T H -1.1029 Soluble
132 K H -1.5779 Soluble
133 G H -1.6829 Soluble
134 P H -0.8881 Soluble
135 S H -0.3805 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2525 Soluble
139 L H 0.0000 Buried
140 A H -1.1998 Soluble
141 P H 0.0000 Buried
142 S H -1.2621 Soluble
143 S H -1.0978 Soluble
144 K H -1.7488 Soluble
145 S H 0.0000 Buried
146 T H -0.9836 Soluble
147 S H -0.8988 Soluble
148 G H -0.8654 Soluble
149 G H -0.8732 Soluble
150 T H -0.5881 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.7945 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4884 Soluble
164 P H -0.7597 Soluble
165 V H 0.0000 Buried
166 T H -0.7734 Soluble
167 V H -0.5194 Soluble
168 S H -0.4327 Soluble
169 W H 0.0000 Buried
170 N H -0.6787 Soluble
171 S H -0.6079 Soluble
172 G H -0.5495 Soluble
173 A H -0.2096 Soluble
174 L H 0.0440 Aggregation-prone
175 T H -0.1573 Soluble
176 S H -0.2016 Soluble
177 G H -0.2866 Soluble
178 V H 0.1441 Aggregation-prone
179 H H -0.3018 Soluble
180 T H 0.0163 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3630 Soluble
183 A H 0.1018 Aggregation-prone
184 V H 0.2582 Aggregation-prone
185 L H 0.8369 Aggregation-prone
186 Q H 0.0837 Aggregation-prone
187 S H -0.1395 Soluble
188 S H -0.2967 Soluble
189 G H -0.0783 Soluble
190 L H -0.0812 Soluble
191 Y H 0.1989 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1549 Soluble
199 V H 0.0000 Buried
200 P H -0.5121 Soluble
201 S H -0.6066 Soluble
202 S H -0.5249 Soluble
203 S H -0.5430 Soluble
204 L H -0.7198 Soluble
205 G H -0.9528 Soluble
206 T H -0.6342 Soluble
207 Q H -1.0625 Soluble
208 T H -1.0404 Soluble
209 Y H 0.0000 Buried
210 I H -1.1832 Soluble
211 C H 0.0000 Buried
212 N H -1.4805 Soluble
213 V H 0.0000 Buried
214 N H -2.3827 Soluble
215 H H 0.0000 Buried
216 K H -2.9838 Soluble
217 P H -1.9929 Soluble
218 S H -2.0640 Soluble
219 N H -2.6036 Soluble
220 T H -2.2361 Soluble
221 K H -2.8198 Soluble
222 V H -1.4404 Soluble
223 D H -2.2888 Soluble
224 K H -1.8570 Soluble
225 K H -2.4951 Soluble
226 V H 0.0000 Buried
227 E H -2.8744 Soluble
228 P H -1.8890 Soluble
229 K H -2.5113 Soluble
230 S H -0.9842 Soluble
231 C H -0.3070 Soluble
1 D L -1.9295 Soluble
2 I L -1.2321 Soluble
3 Q L -1.5926 Soluble
4 M L 0.0000 Buried
5 T L -0.8664 Soluble
6 Q L 0.0000 Buried
7 S L -0.6255 Soluble
8 P L -0.6722 Soluble
9 S L -0.9627 Soluble
10 S L -0.9739 Soluble
11 L L -0.7136 Soluble
12 S L -1.1719 Soluble
13 A L -1.1543 Soluble
14 S L -0.9768 Soluble
15 V L -0.0097 Soluble
16 G L -0.7777 Soluble
17 D L -2.0011 Soluble
18 R L -2.3592 Soluble
19 V L 0.0000 Buried
20 T L -0.6683 Soluble
21 I L 0.0000 Buried
22 T L -0.5531 Soluble
23 C L 0.0000 Buried
24 S L -1.1500 Soluble
25 A L -1.0276 Soluble
26 S L -1.1915 Soluble
27 S L -1.0727 Soluble
28 S L -0.6441 Soluble
29 V L 0.0000 Buried
37 T L -0.1070 Soluble
38 Y L 0.3307 Aggregation-prone
39 M L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.4145 Soluble
45 K L -1.9703 Soluble
46 P L -1.6041 Soluble
47 G L -1.8110 Soluble
48 K L -2.5156 Soluble
49 A L -1.5559 Soluble
50 P L 0.0000 Buried
51 K L -1.6509 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.2578 Soluble
56 D L -0.6826 Soluble
57 T L -0.6154 Soluble
65 S L -0.7406 Soluble
66 N L -0.6733 Soluble
67 L L 0.1217 Aggregation-prone
68 A L -0.3996 Soluble
69 S L -0.6493 Soluble
70 G L -0.4905 Soluble
71 V L 0.0000 Buried
72 P L -0.3600 Soluble
74 S L -0.3990 Soluble
75 R L -0.7851 Soluble
76 F L 0.0000 Buried
77 S L -0.3733 Soluble
78 G L -0.5008 Soluble
79 S L -0.9900 Soluble
80 G L -1.1952 Soluble
83 S L -1.0004 Soluble
84 G L -0.9640 Soluble
85 T L -1.2842 Soluble
86 D L -2.1711 Soluble
87 Y L 0.0000 Buried
88 T L -0.7077 Soluble
89 F L 0.0000 Buried
90 T L -0.6139 Soluble
91 I L 0.0000 Buried
92 S L -1.3543 Soluble
93 S L -1.1457 Soluble
94 L L 0.0000 Buried
95 Q L -0.8171 Soluble
96 P L -1.5652 Soluble
97 E L -1.9412 Soluble
98 D L 0.0000 Buried
99 I L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -1.1108 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 W L 0.3176 Aggregation-prone
108 S L -0.2175 Soluble
109 S L -0.6652 Soluble
114 H L -0.7418 Soluble
115 I L -0.2392 Soluble
116 F L 0.3592 Aggregation-prone
117 T L -0.2393 Soluble
118 F L -0.3879 Soluble
119 G L 0.0000 Buried
120 Q L -1.5423 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.8585 Soluble
124 V L 0.0000 Buried
125 E L -1.3417 Soluble
126 I L 0.0000 Buried
127 K L -1.7568 Soluble
128 R L -0.9709 Soluble
129 T L -0.1049 Soluble
130 V L 0.4897 Aggregation-prone
131 A L 0.2118 Aggregation-prone
132 A L -0.0849 Soluble
133 P L 0.0000 Buried
134 S L -0.2238 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.5421 Soluble
140 P L 0.0000 Buried
141 S L -1.7561 Soluble
142 D L -3.0194 Soluble
143 E L -2.7851 Soluble
144 Q L 0.0000 Buried
145 L L -2.1641 Soluble
146 K L -2.7374 Soluble
147 S L -1.6458 Soluble
148 G L -1.1336 Soluble
149 T L -0.8980 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8907 Soluble
159 F L 0.0000 Buried
160 Y L -0.8598 Soluble
161 P L -1.3784 Soluble
162 R L -2.1896 Soluble
163 E L -2.9049 Soluble
164 A L -2.1377 Soluble
165 K L -2.1098 Soluble
166 V L -1.0500 Soluble
167 Q L -0.5765 Soluble
168 W L 0.0000 Buried
169 K L -0.4829 Soluble
170 V L 0.0000 Buried
171 D L -1.6994 Soluble
172 N L -1.4648 Soluble
173 A L -0.1675 Soluble
174 L L 0.7745 Aggregation-prone
175 Q L -0.0614 Soluble
176 S L -0.4635 Soluble
177 G L -0.8670 Soluble
178 N L -0.6088 Soluble
179 S L -1.1267 Soluble
180 Q L -1.4591 Soluble
181 E L -1.9283 Soluble
182 S L -0.9465 Soluble
183 V L -0.5539 Soluble
184 T L -1.0033 Soluble
185 E L -2.1797 Soluble
186 Q L 0.0000 Buried
187 D L -2.0521 Soluble
188 S L -2.2872 Soluble
189 K L -2.5892 Soluble
190 D L -1.8938 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6785 Soluble
199 L L 0.0000 Buried
200 T L -0.4001 Soluble
201 L L -0.6051 Soluble
202 S L -0.9798 Soluble
203 K L -1.9620 Soluble
204 A L -1.8759 Soluble
205 D L -2.4690 Soluble
206 Y L 0.0000 Buried
207 E L -3.6107 Soluble
208 K L -3.6120 Soluble
209 H L -3.1631 Soluble
210 K L -3.2602 Soluble
211 V L -1.6126 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8167 Soluble
216 V L 0.0000 Buried
217 T L -1.2087 Soluble
218 H L 0.0000 Buried
219 Q L -1.5005 Soluble
220 G L -0.4227 Soluble
221 L L -0.2724 Soluble
222 S L -0.4790 Soluble
223 S L -0.4404 Soluble
224 P L -0.5482 Soluble
225 V L 0.0195 Aggregation-prone
226 T L -0.3838 Soluble
227 K L -0.8137 Soluble
228 S L -0.7170 Soluble
229 F L -1.2403 Soluble
230 N L -2.0261 Soluble
231 R L -2.7998 Soluble
232 G L -2.0129 Soluble
233 E L -2.3798 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8736 1.4865 CSV PDB
4.5 -0.918 1.4515 CSV PDB
5.0 -0.9693 1.408 CSV PDB
5.5 -1.0165 1.3633 CSV PDB
6.0 -1.0472 1.3243 CSV PDB
6.5 -1.0521 1.2974 CSV PDB
7.0 -1.0317 1.2837 CSV PDB
7.5 -0.9947 1.2784 CSV PDB
8.0 -0.9487 1.2765 CSV PDB
8.5 -0.896 1.2759 CSV PDB
9.0 -0.8369 1.2758 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH