Donzakimig1 / 5fuo

A4DAB089_5fuo_H_L | Source PDB: 5fuo | Chains: H, L | Done
99.13
Yes
10 Å
Yes
Avg A4D Score
-0.6669
Max Score
1.2842
Min Score
-3.6727
Total Score
-288.76
Score CDR
-0.3438
Score non-CDR
-0.71

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAVSGIDLSNYAINWVRQAPGKGLEWIGIIWASGTTFYATWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYYCARTVPGYSTAPYFDLWGQGTLVTVSSASTKGPSVFPLAPSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSVSASVGDRVTITCQSSPSVWSNFLSWYQQKPGKAPKLLIYEASKLTSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCGGGYSSISDTTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6727
Maximum Score 1.2842
Average Score -0.6669
Total Score -288.7601

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9806 Soluble
2 V H -1.1695 Soluble
3 Q H -1.1205 Soluble
4 L H 0.0000 Buried
5 L H 0.2891 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.6253 Soluble
8 G H -0.9215 Soluble
9 G H -0.5765 Soluble
11 G H -0.1360 Soluble
12 L H -0.1811 Soluble
13 V H 0.0000 Buried
14 Q H -1.7957 Soluble
15 P H -2.0091 Soluble
16 G H -1.6782 Soluble
17 G H -1.3557 Soluble
18 S H -1.4077 Soluble
19 L H -1.2171 Soluble
20 R H -2.0988 Soluble
21 L H 0.0000 Buried
22 S H -0.5795 Soluble
23 C H 0.0000 Buried
24 A H -0.4169 Soluble
25 V H 0.0000 Buried
26 S H -0.9899 Soluble
27 G H -1.3343 Soluble
28 I H -1.2013 Soluble
29 D H -2.0947 Soluble
30 L H 0.0000 Buried
35 S H -1.8964 Soluble
36 N H -1.6609 Soluble
37 Y H -0.6129 Soluble
38 A H 0.0000 Buried
39 I H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.5488 Soluble
44 Q H -0.8837 Soluble
45 A H -1.2378 Soluble
46 P H -0.9753 Soluble
47 G H -1.4754 Soluble
48 K H -2.2779 Soluble
49 G H -1.4619 Soluble
50 L H 0.0000 Buried
51 E H -1.0044 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 I H 0.0000 Buried
56 I H 0.0000 Buried
57 W H -0.0871 Soluble
58 A H -0.6974 Soluble
59 S H -0.5772 Soluble
63 G H -0.5865 Soluble
64 T H -0.1468 Soluble
65 T H 0.1444 Aggregation-prone
66 F H 0.4350 Aggregation-prone
67 Y H -0.1651 Soluble
68 A H 0.0000 Buried
69 T H -0.3318 Soluble
70 W H -0.1183 Soluble
71 A H 0.0000 Buried
72 K H -1.7384 Soluble
74 G H -1.4338 Soluble
75 R H -1.4066 Soluble
76 F H 0.0000 Buried
77 T H -0.8688 Soluble
78 I H 0.0000 Buried
79 S H -0.5453 Soluble
80 R H -1.3845 Soluble
81 D H -2.1134 Soluble
82 N H -2.9532 Soluble
83 S H -2.0819 Soluble
84 K H -2.7994 Soluble
85 N H -2.5685 Soluble
86 T H -1.3779 Soluble
87 V H 0.0000 Buried
88 Y H -0.5827 Soluble
89 L H 0.0000 Buried
90 Q H -1.2669 Soluble
91 M H 0.0000 Buried
92 N H -1.5943 Soluble
93 S H -1.4942 Soluble
94 L H 0.0000 Buried
95 R H -2.9356 Soluble
96 A H -2.0176 Soluble
97 E H -2.4133 Soluble
98 D H 0.0000 Buried
99 T H -0.8422 Soluble
100 A H 0.0000 Buried
101 V H 0.1173 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 T H 0.0000 Buried
108 V H 0.0834 Aggregation-prone
109 P H -0.3734 Soluble
110 G H -0.2693 Soluble
111 Y H 0.1768 Aggregation-prone
111A S H 0.1255 Aggregation-prone
112A T H 0.1758 Aggregation-prone
112 A H 0.0000 Buried
113 P H 0.0000 Buried
114 Y H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.6070 Soluble
117 L H -0.2914 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.2855 Soluble
121 G H -0.6687 Soluble
122 T H -0.2016 Soluble
123 L H -0.0082 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.1623 Soluble
128 S H -1.0432 Soluble
129 A H -0.5766 Soluble
130 S H -0.6531 Soluble
131 T H -0.7165 Soluble
132 K H -1.4040 Soluble
133 G H -1.4797 Soluble
134 P H 0.0000 Buried
135 S H -0.5325 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H 0.0000 Buried
139 L H 0.0000 Buried
140 A H -1.1285 Soluble
141 P H 0.0000 Buried
142 S H -0.3474 Soluble
143 T H -0.2502 Soluble
144 A H -0.1313 Soluble
145 A H 0.0000 Buried
146 L H 0.0000 Buried
147 G H 0.0000 Buried
148 C H 0.0000 Buried
149 L H 0.0000 Buried
150 V H 0.0000 Buried
151 K H 0.0000 Buried
152 D H -0.4053 Soluble
153 Y H 0.0000 Buried
154 F H 0.0000 Buried
155 P H 0.0000 Buried
156 E H -0.3150 Soluble
157 P H -0.6388 Soluble
158 V H -0.5727 Soluble
159 T H -0.4660 Soluble
160 V H -0.3083 Soluble
161 S H -0.3482 Soluble
162 W H 0.0000 Buried
163 N H -0.7150 Soluble
164 S H -0.6407 Soluble
165 G H -0.4153 Soluble
166 A H -0.1738 Soluble
167 L H 0.0710 Aggregation-prone
168 T H -0.1352 Soluble
169 S H -0.3336 Soluble
170 G H -0.1569 Soluble
171 V H 0.1758 Aggregation-prone
172 H H -0.2173 Soluble
173 T H 0.0836 Aggregation-prone
174 F H 0.0000 Buried
175 P H -0.1857 Soluble
176 A H 0.4542 Aggregation-prone
177 V H 0.9040 Aggregation-prone
178 L H 1.2842 Aggregation-prone
179 Q H 0.2899 Aggregation-prone
180 S H -0.3537 Soluble
181 S H -0.2845 Soluble
182 G H -0.0817 Soluble
183 L H 0.1091 Aggregation-prone
184 Y H 0.4610 Aggregation-prone
185 S H 0.3467 Aggregation-prone
186 L H 0.0000 Buried
187 S H 0.0000 Buried
188 S H 0.0000 Buried
189 V H 0.0000 Buried
190 V H 0.0000 Buried
191 T H -0.1033 Soluble
192 V H 0.0000 Buried
193 P H -0.4184 Soluble
194 S H -0.3624 Soluble
195 S H -0.4821 Soluble
196 S H -0.5601 Soluble
197 L H -0.7138 Soluble
198 G H -1.0365 Soluble
199 T H -0.8450 Soluble
200 Q H -1.1811 Soluble
201 T H -1.0283 Soluble
202 Y H 0.0000 Buried
203 I H -1.2792 Soluble
204 C H 0.0000 Buried
205 N H -1.4947 Soluble
206 V H 0.0000 Buried
207 N H -1.7100 Soluble
208 H H 0.0000 Buried
209 K H -2.7577 Soluble
210 P H -1.6416 Soluble
211 S H -1.8960 Soluble
212 N H -2.6700 Soluble
213 T H -2.0786 Soluble
214 K H -2.5966 Soluble
215 V H -1.5464 Soluble
216 D H -2.5127 Soluble
217 K H -2.1654 Soluble
218 K H -2.3816 Soluble
219 V H 0.0000 Buried
220 E H -2.3492 Soluble
221 P H -1.8435 Soluble
222 K H -2.2944 Soluble
223 S H -1.3301 Soluble
1 D L -2.0539 Soluble
2 I L -1.3773 Soluble
3 Q L -1.8914 Soluble
4 M L 0.0000 Buried
5 T L -1.0560 Soluble
6 Q L 0.0000 Buried
7 S L -0.5869 Soluble
8 P L -0.4176 Soluble
9 S L -0.6055 Soluble
10 S L -0.5456 Soluble
11 V L -0.0863 Soluble
12 S L -0.2652 Soluble
13 A L -0.8748 Soluble
14 S L -0.9833 Soluble
15 V L -0.4863 Soluble
16 G L -0.9995 Soluble
17 D L -2.1205 Soluble
18 R L -2.5215 Soluble
19 V L 0.0000 Buried
20 T L -0.6187 Soluble
21 I L 0.0000 Buried
22 T L -0.6703 Soluble
23 C L 0.0000 Buried
24 Q L -1.8535 Soluble
25 S L 0.0000 Buried
26 S L -1.3653 Soluble
27 P L -0.8152 Soluble
28 S L -0.6496 Soluble
29 V L 0.0000 Buried
30 W L 0.2532 Aggregation-prone
36 S L -0.4479 Soluble
37 N L -1.0620 Soluble
38 F L -0.2581 Soluble
39 L L 0.0000 Buried
40 S L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.3190 Soluble
45 K L -1.9569 Soluble
46 P L -1.5962 Soluble
47 G L -1.6564 Soluble
48 K L -2.5391 Soluble
49 A L -1.5208 Soluble
50 P L 0.0000 Buried
51 K L -1.3202 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.4292 Soluble
56 E L -1.0029 Soluble
57 A L 0.0000 Buried
65 S L -1.0449 Soluble
66 K L -1.2438 Soluble
67 L L -0.3564 Soluble
68 T L -0.4320 Soluble
69 S L -0.4294 Soluble
70 G L -0.4538 Soluble
71 V L -0.2429 Soluble
72 P L -0.2869 Soluble
74 S L -0.4095 Soluble
75 R L -0.8336 Soluble
76 F L 0.0000 Buried
77 S L -0.5200 Soluble
78 G L -0.5592 Soluble
79 S L -0.8662 Soluble
80 G L -1.2169 Soluble
83 S L -1.1839 Soluble
84 G L -0.9983 Soluble
85 T L -1.1957 Soluble
86 D L -1.9190 Soluble
87 F L 0.0000 Buried
88 T L -0.6922 Soluble
89 L L 0.0000 Buried
90 T L -0.6327 Soluble
91 I L 0.0000 Buried
92 S L -1.4746 Soluble
93 S L -1.3618 Soluble
94 L L 0.0000 Buried
95 Q L -1.1887 Soluble
96 P L -1.3916 Soluble
97 E L -2.1109 Soluble
98 D L 0.0000 Buried
99 F L -1.1397 Soluble
100 A L 0.0000 Buried
101 T L -0.6804 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 G L 0.0000 Buried
106 G L 0.0000 Buried
107 G L 0.0000 Buried
108 Y L 0.0000 Buried
109 S L -0.0253 Soluble
110 S L -0.0991 Soluble
113 I L 0.2644 Aggregation-prone
114 S L -0.0900 Soluble
115 D L -0.3659 Soluble
116 T L 0.0000 Buried
117 T L -0.4575 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.1097 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.0250 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L 0.0000 Buried
127 K L -1.0791 Soluble
128 R L -0.6684 Soluble
129 T L 0.2027 Aggregation-prone
130 V L 1.0774 Aggregation-prone
131 A L 0.3827 Aggregation-prone
132 A L 0.0645 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.0917 Soluble
135 V L -0.0310 Soluble
136 F L -0.0392 Soluble
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.7311 Soluble
140 P L -0.6799 Soluble
141 S L 0.0000 Buried
142 D L -2.6452 Soluble
143 E L -1.9528 Soluble
144 Q L 0.0000 Buried
145 L L -1.9418 Soluble
146 K L -2.4878 Soluble
147 S L -1.5084 Soluble
148 G L -1.2160 Soluble
149 T L -1.1068 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6299 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -0.9629 Soluble
162 R L -1.7107 Soluble
163 E L -2.3827 Soluble
164 A L -1.9603 Soluble
165 K L -2.2911 Soluble
166 V L -1.0696 Soluble
167 Q L -0.7019 Soluble
168 W L 0.0000 Buried
169 K L -0.5975 Soluble
170 V L 0.0000 Buried
171 D L -1.7254 Soluble
172 N L -1.3113 Soluble
173 A L -0.3109 Soluble
174 L L 0.5180 Aggregation-prone
175 Q L -0.3883 Soluble
176 S L -0.7948 Soluble
177 G L -1.3590 Soluble
178 N L -1.7402 Soluble
179 S L -1.5048 Soluble
180 Q L -1.2903 Soluble
181 E L -1.2834 Soluble
182 S L -0.2746 Soluble
183 V L 0.0212 Aggregation-prone
184 T L -0.7520 Soluble
185 E L -1.9619 Soluble
186 Q L 0.0000 Buried
187 D L -1.7125 Soluble
188 S L -1.9110 Soluble
189 K L -2.3164 Soluble
190 D L -1.4782 Soluble
191 S L -1.5395 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7504 Soluble
199 L L 0.0000 Buried
200 T L -0.7528 Soluble
201 L L -1.0086 Soluble
202 S L -1.3214 Soluble
203 K L -2.1831 Soluble
204 A L -2.0729 Soluble
205 D L -2.7622 Soluble
206 Y L 0.0000 Buried
207 E L -3.5710 Soluble
208 K L -3.6727 Soluble
209 H L -2.9195 Soluble
210 K L -2.7723 Soluble
211 V L -1.1636 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7026 Soluble
216 V L 0.0000 Buried
217 T L -1.1558 Soluble
218 H L 0.0000 Buried
219 Q L -1.5182 Soluble
220 G L -0.3529 Soluble
221 L L -0.0656 Soluble
222 S L -0.4029 Soluble
223 S L -0.3394 Soluble
224 P L -0.4762 Soluble
225 V L 0.0792 Aggregation-prone
226 T L -0.4730 Soluble
227 K L -0.8694 Soluble
228 S L -0.8038 Soluble
229 F L -0.7959 Soluble
230 N L -1.6580 Soluble
231 R L -2.4426 Soluble
232 G L -2.0458 Soluble
233 E L -2.1610 Soluble
234 C L -0.7951 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6967 1.49 CSV PDB
4.5 -0.7357 1.4708 CSV PDB
5.0 -0.7802 1.4474 CSV PDB
5.5 -0.8214 1.4346 CSV PDB
6.0 -0.8488 1.4436 CSV PDB
6.5 -0.8534 1.4748 CSV PDB
7.0 -0.8354 1.5264 CSV PDB
7.5 -0.8028 1.5935 CSV PDB
8.0 -0.7623 1.6688 CSV PDB
8.5 -0.7158 1.7465 CSV PDB
9.0 -0.6632 1.8226 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH