Vilastobart / 8g2m

A4DAB294_8g2m_H_L | Source PDB: 8g2m | Chains: H, L | Done
98.18
Yes
10 Å
Yes
Avg A4D Score
-0.8184
Max Score
1.187
Min Score
-4.1617
Total Score
-341.27
Score CDR
-0.6201
Score non-CDR
-0.8406

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGSSVKVSCKASGYTFTNYFMNWVRQAPGQGLEWMGRVDPEQGRADYAEKFKKRVTITADKSTSTAYMELSSLRSEDTAVYYCARRAMDNYGFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSAGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKAFQSVTPKEKVTITCSANGYMYWYQQKPDQSPKLWVHGTSNLASGVPSRFSGSGSGTDFTLTINSLEAEDAATYYCHHWSNTQWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.1617
Maximum Score 1.187
Average Score -0.8184
Total Score -341.2726

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.3989 Soluble
2 V H -0.7388 Soluble
3 Q H -1.3378 Soluble
4 L H 0.0000 Buried
5 V H -0.2104 Soluble
6 Q H 0.0000 Buried
7 S H -0.6027 Soluble
8 G H -0.5438 Soluble
9 A H 0.1140 Aggregation-prone
11 E H -0.3897 Soluble
12 V H 0.7639 Aggregation-prone
13 K H -0.9475 Soluble
14 K H -2.1068 Soluble
15 P H -2.0910 Soluble
16 G H -1.4862 Soluble
17 S H -1.1509 Soluble
19 V H -0.9180 Soluble
20 K H -1.8622 Soluble
21 V H 0.0000 Buried
22 S H -0.6426 Soluble
23 C H 0.0000 Buried
24 K H -1.2269 Soluble
25 A H 0.0000 Buried
26 S H -0.7806 Soluble
27 G H -0.6983 Soluble
28 Y H -0.3773 Soluble
29 T H -0.3565 Soluble
30 F H 0.0000 Buried
35 T H -1.8637 Soluble
36 N H -1.4554 Soluble
37 Y H -0.4902 Soluble
38 F H -0.3044 Soluble
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.4864 Soluble
44 Q H -0.6619 Soluble
45 A H -0.9818 Soluble
46 P H -0.9540 Soluble
47 G H -1.2480 Soluble
48 Q H -1.8008 Soluble
49 G H -1.2410 Soluble
50 L H 0.0000 Buried
51 E H -0.8746 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 R H -0.5028 Soluble
56 V H 0.0000 Buried
57 D H -2.0429 Soluble
58 P H 0.0000 Buried
59 E H -3.4852 Soluble
62 Q H -3.1483 Soluble
63 G H -2.8946 Soluble
64 R H -2.9324 Soluble
65 A H -1.4658 Soluble
66 D H -1.4253 Soluble
67 Y H -1.4853 Soluble
68 A H 0.0000 Buried
69 E H -3.3656 Soluble
70 K H -3.2460 Soluble
71 F H -2.7406 Soluble
72 K H -3.6398 Soluble
74 K H -3.4281 Soluble
75 R H -2.3608 Soluble
76 V H -1.6690 Soluble
77 T H -1.2669 Soluble
78 I H 0.0000 Buried
79 T H -0.9671 Soluble
80 A H -1.7257 Soluble
81 D H -2.1660 Soluble
82 K H -2.7952 Soluble
83 S H -1.3432 Soluble
84 T H -1.1774 Soluble
85 S H -1.3382 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.7201 Soluble
89 M H 0.0000 Buried
90 E H -1.7580 Soluble
91 L H 0.0000 Buried
92 S H -1.5538 Soluble
93 S H -1.3976 Soluble
94 L H 0.0000 Buried
95 R H -2.7743 Soluble
96 S H -2.2487 Soluble
97 E H -2.5158 Soluble
98 D H -1.7195 Soluble
99 T H -0.7156 Soluble
100 A H 0.0000 Buried
101 V H 0.3540 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 R H 0.0209 Aggregation-prone
108 A H 0.0000 Buried
109 M H 0.2367 Aggregation-prone
110 D H -1.2656 Soluble
112 N H -0.5992 Soluble
113 Y H 0.6399 Aggregation-prone
114 G H 0.0000 Buried
115 F H 0.0000 Buried
116 A H -0.1685 Soluble
117 Y H -0.0168 Soluble
118 W H -0.4336 Soluble
119 G H 0.0000 Buried
120 Q H -1.4346 Soluble
121 G H -0.6617 Soluble
122 T H 0.0000 Buried
123 L H 0.9956 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.2234 Soluble
126 V H 0.0000 Buried
127 S H -1.0714 Soluble
128 S H -0.9798 Soluble
129 A H -0.5150 Soluble
130 S H -0.5726 Soluble
131 T H -0.5049 Soluble
132 K H -1.1508 Soluble
133 G H -1.4032 Soluble
134 P H 0.0000 Buried
135 S H -0.3900 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2648 Soluble
139 L H 0.0000 Buried
140 A H -1.2516 Soluble
141 P H 0.0000 Buried
142 S H -1.2491 Soluble
143 S H -1.6361 Soluble
144 K H -1.9120 Soluble
145 S H -1.1396 Soluble
146 A H -0.8222 Soluble
147 G H -0.8474 Soluble
148 G H -0.6807 Soluble
149 T H -0.5193 Soluble
150 A H 0.0000 Buried
151 A H 0.0000 Buried
152 L H 0.0000 Buried
153 G H 0.0000 Buried
154 C H 0.0000 Buried
155 L H 0.0000 Buried
156 V H 0.0000 Buried
157 K H 0.0000 Buried
158 D H -0.4139 Soluble
159 Y H 0.0000 Buried
160 F H 0.0000 Buried
161 P H 0.0000 Buried
162 E H -0.9304 Soluble
163 P H -1.0835 Soluble
164 V H -0.9505 Soluble
165 T H -0.8022 Soluble
166 V H -0.3125 Soluble
167 S H -0.4497 Soluble
168 W H 0.0000 Buried
169 N H -0.7377 Soluble
170 S H -0.6693 Soluble
171 G H -0.5583 Soluble
172 A H -0.2334 Soluble
173 L H 0.0041 Aggregation-prone
174 T H -0.1702 Soluble
175 S H -0.1994 Soluble
176 G H -0.2464 Soluble
177 V H 0.1767 Aggregation-prone
178 H H -0.2606 Soluble
179 T H 0.0599 Aggregation-prone
180 F H 0.0000 Buried
181 P H -0.2815 Soluble
182 A H 0.1583 Aggregation-prone
183 V H 0.4170 Aggregation-prone
184 L H 1.1478 Aggregation-prone
185 Q H 0.1995 Aggregation-prone
186 S H -0.0670 Soluble
187 S H -0.2083 Soluble
188 G H 0.0055 Aggregation-prone
189 L H 0.0319 Aggregation-prone
190 Y H 0.3006 Aggregation-prone
191 S H 0.0000 Buried
192 L H 0.0000 Buried
193 S H 0.0000 Buried
194 S H 0.0000 Buried
195 V H 0.0000 Buried
196 V H 0.0000 Buried
197 T H -0.1368 Soluble
198 V H 0.0000 Buried
199 P H -0.5747 Soluble
200 S H -0.6922 Soluble
201 S H -0.5622 Soluble
202 S H -0.6298 Soluble
203 L H -0.8629 Soluble
204 G H -0.9830 Soluble
205 T H -0.7312 Soluble
206 Q H -1.2585 Soluble
207 T H -1.0846 Soluble
208 Y H 0.0000 Buried
209 I H -1.1875 Soluble
210 C H 0.0000 Buried
211 N H -1.5827 Soluble
212 V H 0.0000 Buried
213 N H -2.4721 Soluble
214 H H 0.0000 Buried
215 K H -2.9547 Soluble
216 P H -1.7418 Soluble
217 S H -1.8798 Soluble
218 N H -2.7019 Soluble
219 T H -2.2376 Soluble
220 K H -2.9635 Soluble
221 V H -1.7964 Soluble
222 D H -2.6220 Soluble
223 K H -2.1496 Soluble
224 K H -2.3116 Soluble
225 V H 0.0000 Buried
226 E H -2.8432 Soluble
227 P H -1.9918 Soluble
228 K H -2.6753 Soluble
229 A H -1.2869 Soluble
10 F L 1.0798 Aggregation-prone
11 Q L -0.1537 Soluble
12 S L -0.5583 Soluble
13 V L 0.0000 Buried
14 T L -2.0994 Soluble
15 P L -2.6152 Soluble
16 K L -3.6847 Soluble
17 E L -4.1617 Soluble
18 K L -3.5881 Soluble
19 V L 0.0000 Buried
20 T L -0.6011 Soluble
21 I L 0.0000 Buried
22 T L -0.4247 Soluble
23 C L -0.4283 Soluble
25 A L -1.0802 Soluble
26 N L -1.6391 Soluble
27 G L 0.0176 Aggregation-prone
38 Y L 1.1870 Aggregation-prone
39 M L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -2.1658 Soluble
46 P L -2.1203 Soluble
47 D L -2.8280 Soluble
48 Q L -2.8245 Soluble
49 S L -1.7859 Soluble
50 P L 0.0000 Buried
51 K L -1.7123 Soluble
52 L L 0.0000 Buried
53 W L 0.0000 Buried
54 V L 0.0000 Buried
55 H L -0.3520 Soluble
56 G L -0.0265 Soluble
57 T L -0.2147 Soluble
65 S L -0.6996 Soluble
66 N L -1.2555 Soluble
67 L L -0.4497 Soluble
68 A L 0.0000 Buried
69 S L -0.6276 Soluble
70 G L -0.5381 Soluble
71 V L 0.0000 Buried
72 P L -0.3756 Soluble
74 S L -0.5974 Soluble
75 R L -1.3340 Soluble
76 F L 0.0000 Buried
78 G L -0.5452 Soluble
79 S L -0.8269 Soluble
80 G L -0.8205 Soluble
83 S L -1.0833 Soluble
84 G L -1.3596 Soluble
85 T L -1.5846 Soluble
86 D L -2.1593 Soluble
87 F L -0.9506 Soluble
88 T L -0.5349 Soluble
89 L L 0.0000 Buried
90 T L -0.8740 Soluble
91 I L 0.0000 Buried
92 N L -3.1546 Soluble
93 S L -3.2766 Soluble
94 L L 0.0000 Buried
95 E L -2.8732 Soluble
96 A L -1.6883 Soluble
97 E L -2.2418 Soluble
98 D L 0.0000 Buried
99 A L -1.2145 Soluble
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 H L 0.0000 Buried
106 H L 0.1582 Aggregation-prone
107 W L 0.2251 Aggregation-prone
108 S L -0.8117 Soluble
109 N L -1.2725 Soluble
114 T L -0.9641 Soluble
115 Q L -0.8007 Soluble
116 W L 0.0000 Buried
117 T L -0.1764 Soluble
118 F L -0.1010 Soluble
119 G L -0.3908 Soluble
120 G L -1.0436 Soluble
121 G L -0.8779 Soluble
122 T L -0.5478 Soluble
123 K L -1.0851 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -1.1605 Soluble
127 K L -1.5173 Soluble
128 R L -0.9920 Soluble
129 T L -0.0879 Soluble
130 V L 0.4457 Aggregation-prone
131 A L 0.1497 Aggregation-prone
132 A L -0.1067 Soluble
133 P L 0.0000 Buried
134 S L -0.1879 Soluble
135 V L 0.0000 Buried
136 F L 0.1290 Aggregation-prone
137 I L 0.0912 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.7224 Soluble
140 P L -0.7943 Soluble
141 S L 0.0000 Buried
142 D L -2.9704 Soluble
143 E L -2.7365 Soluble
144 Q L 0.0000 Buried
145 L L -2.1130 Soluble
146 K L -2.7159 Soluble
147 S L -1.6640 Soluble
148 G L -1.3046 Soluble
149 T L -0.9532 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7458 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.2856 Soluble
162 R L -2.0900 Soluble
163 E L -2.7784 Soluble
164 A L -2.0847 Soluble
165 K L -2.2920 Soluble
166 V L -0.9695 Soluble
167 Q L -0.4274 Soluble
168 W L 0.0000 Buried
169 K L -0.4399 Soluble
170 V L 0.0000 Buried
171 D L -1.7979 Soluble
172 N L -1.4817 Soluble
173 A L -0.2031 Soluble
174 L L 0.7703 Aggregation-prone
175 Q L -0.0498 Soluble
176 S L -0.3703 Soluble
177 G L -0.9549 Soluble
178 N L -0.8836 Soluble
179 S L -1.2575 Soluble
180 Q L -1.8057 Soluble
181 E L -1.9816 Soluble
182 S L -0.7480 Soluble
183 V L -0.4210 Soluble
184 T L -0.9672 Soluble
185 E L -2.1233 Soluble
186 Q L -1.7910 Soluble
187 D L -1.9644 Soluble
188 S L -1.8856 Soluble
189 K L -2.2242 Soluble
190 D L -1.6601 Soluble
191 S L -1.6593 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8044 Soluble
199 L L 0.0000 Buried
200 T L -0.4873 Soluble
201 L L -0.6597 Soluble
202 S L -1.0211 Soluble
203 K L -1.9813 Soluble
204 A L -1.8400 Soluble
205 D L -2.5640 Soluble
206 Y L 0.0000 Buried
207 E L -3.3819 Soluble
208 K L -3.5529 Soluble
209 H L -3.0126 Soluble
210 K L -2.9616 Soluble
211 V L -1.2683 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7400 Soluble
216 V L 0.0000 Buried
217 T L -1.2180 Soluble
218 H L 0.0000 Buried
219 Q L -1.6317 Soluble
220 G L -0.4245 Soluble
221 L L -0.2699 Soluble
222 S L -0.4929 Soluble
223 S L -0.4521 Soluble
224 P L -0.6059 Soluble
225 V L -0.0486 Soluble
226 T L -0.4679 Soluble
227 K L -0.6525 Soluble
228 S L -0.5275 Soluble
229 F L -0.8713 Soluble
230 N L -1.7214 Soluble
231 R L -2.5050 Soluble
232 G L -2.3092 Soluble
233 E L -2.6597 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8157 2.7946 CSV PDB
4.5 -0.8649 2.796 CSV PDB
5.0 -0.9251 2.8005 CSV PDB
5.5 -0.9832 2.8137 CSV PDB
6.0 -1.0231 2.8485 CSV PDB
6.5 -1.0315 2.9216 CSV PDB
7.0 -1.0068 3.0352 CSV PDB
7.5 -0.9593 3.1737 CSV PDB
8.0 -0.8994 3.3229 CSV PDB
8.5 -0.8318 3.4756 CSV PDB
9.0 -0.7579 3.6288 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH