Romlusevimab / 8gx9

A4DAB227_8gx9_O_N | Source PDB: 8gx9 | Chains: O, N | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.5835
Max Score
1.6481
Min Score
-3.2056
Total Score
-247.39
Score CDR
-0.1868
Score non-CDR
-0.6341

Calculation Parameters

Chain Sequence(s) VQLVQSGSELKKPGASVKVSCKASGYTFTTYVMNWVRQAPGQGLEWMGWINTNTGNPTYAQGFTGRFVFSLDTSVSTASLQISSLKAEDTAVYYCSCEITTLGGMDVWGQGTTVTVSSASTKGPSVFPLAPSSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVESYVLTQPPSVSVAPGKTARITCGGNNIGSKSVHWYQQKPGQAPVLVIYYDSDRPSGIPERFSGSNSGNTATLTISGVEAGDEADYYCQVWDSISDHRVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP input.pdb
Selected Chain(s) O (Heavy), N (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2056
Maximum Score 1.6481
Average Score -0.5835
Total Score -247.3863

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V O 1.0951 Aggregation-prone
3 Q O -0.2892 Soluble
4 L O 0.0000 Buried
5 V O 0.0810 Aggregation-prone
6 Q O 0.0000 Buried
7 S O -0.7252 Soluble
8 G O -0.5172 Soluble
9 S O -0.4472 Soluble
11 E O -0.6330 Soluble
12 L O -0.0283 Soluble
13 K O -1.0104 Soluble
14 K O -1.6056 Soluble
15 P O -1.5510 Soluble
16 G O -1.2483 Soluble
17 A O -0.8889 Soluble
18 S O -1.1501 Soluble
19 V O 0.0000 Buried
20 K O -1.8750 Soluble
21 V O 0.0000 Buried
22 S O -0.8043 Soluble
23 C O 0.0000 Buried
24 K O -0.8245 Soluble
25 A O -0.1535 Soluble
26 S O -0.0169 Soluble
27 G O 0.1076 Aggregation-prone
28 Y O 0.4227 Aggregation-prone
29 T O 0.3025 Aggregation-prone
30 F O 0.1560 Aggregation-prone
35 T O -0.3044 Soluble
36 T O 0.1636 Aggregation-prone
37 Y O 0.7158 Aggregation-prone
38 V O 0.5478 Aggregation-prone
39 M O 0.0000 Buried
40 N O 0.0000 Buried
41 W O 0.0000 Buried
42 V O 0.0000 Buried
43 R O -0.8648 Soluble
44 Q O -0.9682 Soluble
45 A O -1.1461 Soluble
46 P O -1.0419 Soluble
47 G O -1.2112 Soluble
48 Q O -1.8881 Soluble
49 G O -1.3737 Soluble
50 L O 0.0000 Buried
51 E O -1.6730 Soluble
52 W O 0.0000 Buried
53 M O 0.0000 Buried
54 G O 0.0000 Buried
55 W O -0.1949 Soluble
56 I O 0.0000 Buried
57 N O -1.1108 Soluble
58 T O 0.0000 Buried
59 N O -1.5583 Soluble
62 T O -1.3269 Soluble
63 G O -1.3561 Soluble
64 N O -1.7677 Soluble
65 P O -0.6316 Soluble
66 T O -0.4548 Soluble
67 Y O -0.0675 Soluble
68 A O -0.8903 Soluble
69 Q O -1.4784 Soluble
70 G O -1.1326 Soluble
71 F O 0.0000 Buried
72 T O -0.5962 Soluble
74 G O -0.6881 Soluble
75 R O -0.5637 Soluble
76 F O 0.0000 Buried
77 V O 0.5479 Aggregation-prone
78 F O 0.0000 Buried
79 S O -0.3992 Soluble
80 L O -0.3625 Soluble
81 D O -0.8458 Soluble
82 T O -0.2698 Soluble
83 S O 0.2316 Aggregation-prone
84 V O 1.0940 Aggregation-prone
85 S O 0.1556 Aggregation-prone
86 T O -0.1760 Soluble
87 A O 0.0000 Buried
88 S O 0.0000 Buried
89 L O 0.0000 Buried
90 Q O -0.8968 Soluble
91 I O 0.0000 Buried
92 S O -0.7928 Soluble
93 S O -0.9654 Soluble
94 L O 0.0000 Buried
95 K O -1.9684 Soluble
96 A O -1.6499 Soluble
97 E O -2.1356 Soluble
98 D O 0.0000 Buried
99 T O -0.7896 Soluble
100 A O 0.0000 Buried
101 V O 0.0659 Aggregation-prone
102 Y O 0.0000 Buried
103 Y O 0.0000 Buried
104 C O 0.0000 Buried
105 S O 0.0000 Buried
106 C O 0.0000 Buried
107 E O 0.0000 Buried
108 I O 1.5909 Aggregation-prone
109 T O 0.9257 Aggregation-prone
110 T O 0.5125 Aggregation-prone
112 L O 0.8552 Aggregation-prone
113 G O 0.7901 Aggregation-prone
114 G O 0.0000 Buried
115 M O 0.0000 Buried
116 D O 0.2227 Aggregation-prone
117 V O 0.5003 Aggregation-prone
118 W O 0.0939 Aggregation-prone
119 G O 0.0000 Buried
120 Q O -1.1771 Soluble
121 G O -0.7390 Soluble
122 T O 0.0000 Buried
123 T O -0.2022 Soluble
124 V O 0.0000 Buried
125 T O -0.4666 Soluble
126 V O 0.0000 Buried
127 S O -0.8094 Soluble
128 S O -0.8805 Soluble
129 A O -0.5326 Soluble
130 S O -0.6777 Soluble
131 T O -0.7730 Soluble
132 K O -1.2479 Soluble
133 G O -1.3736 Soluble
134 P O -0.5321 Soluble
135 S O -0.1970 Soluble
136 V O 0.0000 Buried
137 F O 0.0000 Buried
138 P O 0.0000 Buried
139 L O 0.0000 Buried
140 A O -0.8257 Soluble
141 P O -0.5067 Soluble
142 S O -0.5059 Soluble
143 S O -0.3615 Soluble
144 S O -0.6554 Soluble
145 G O -0.8505 Soluble
146 G O -0.9192 Soluble
147 T O -0.6075 Soluble
148 A O -0.3790 Soluble
149 A O -0.1853 Soluble
150 L O 0.0000 Buried
151 G O 0.0000 Buried
152 C O 0.0000 Buried
153 L O 0.0000 Buried
154 V O 0.0000 Buried
155 K O 0.0000 Buried
156 D O -0.1750 Soluble
157 Y O 0.0000 Buried
158 F O 0.0000 Buried
159 P O -0.6507 Soluble
160 E O -0.9263 Soluble
161 P O -1.0709 Soluble
162 V O -0.8285 Soluble
163 T O -0.6303 Soluble
164 V O -0.3223 Soluble
165 S O -0.3804 Soluble
166 W O 0.0000 Buried
167 N O -0.7450 Soluble
168 S O -0.6846 Soluble
169 G O -0.5281 Soluble
170 A O -0.2446 Soluble
171 L O -0.0848 Soluble
172 T O -0.2253 Soluble
173 S O -0.2897 Soluble
174 G O -0.3706 Soluble
175 V O 0.1315 Aggregation-prone
176 H O -0.1675 Soluble
177 T O -0.0241 Soluble
178 F O 0.0000 Buried
179 P O -0.0730 Soluble
180 A O 0.1551 Aggregation-prone
181 V O 0.0000 Buried
182 L O 1.2219 Aggregation-prone
183 Q O 0.0000 Buried
184 S O -0.0187 Soluble
185 S O -0.0723 Soluble
186 G O 0.1737 Aggregation-prone
187 L O 0.2088 Aggregation-prone
188 Y O 0.3670 Aggregation-prone
189 S O 0.0000 Buried
190 L O 0.0000 Buried
191 S O 0.0000 Buried
192 S O 0.0000 Buried
193 V O 0.0000 Buried
194 V O 0.0000 Buried
195 T O -0.2383 Soluble
196 V O 0.0000 Buried
197 P O -0.5994 Soluble
198 S O -0.5432 Soluble
199 S O -0.5363 Soluble
200 S O -0.5617 Soluble
201 L O -0.5195 Soluble
202 G O -0.8618 Soluble
203 T O -0.7283 Soluble
204 Q O -1.1718 Soluble
205 T O -1.1289 Soluble
206 Y O 0.0000 Buried
207 I O -1.1659 Soluble
208 C O 0.0000 Buried
209 N O -1.3474 Soluble
210 V O 0.0000 Buried
211 N O -1.8125 Soluble
212 H O 0.0000 Buried
213 K O -2.7194 Soluble
214 P O -1.6879 Soluble
215 S O -1.8129 Soluble
216 N O -2.4806 Soluble
217 T O -2.0258 Soluble
218 K O -2.6041 Soluble
219 V O -1.4601 Soluble
220 D O -2.4228 Soluble
221 K O -2.0033 Soluble
222 K O -2.3737 Soluble
223 V O 0.0000 Buried
224 E O -2.2016 Soluble
1 S N 0.0413 Aggregation-prone
2 Y N 0.8254 Aggregation-prone
3 V N 1.6481 Aggregation-prone
4 L N 0.7318 Aggregation-prone
5 T N 0.1461 Aggregation-prone
6 Q N -0.3907 Soluble
7 P N -0.5844 Soluble
8 P N -0.8768 Soluble
9 S N -0.9489 Soluble
11 V N -0.7887 Soluble
12 S N -0.4237 Soluble
13 V N 0.0000 Buried
14 A N -0.7443 Soluble
15 P N -1.3324 Soluble
16 G N -1.9543 Soluble
17 K N -2.3591 Soluble
18 T N -1.8214 Soluble
19 A N 0.0000 Buried
20 R N -1.8930 Soluble
21 I N 0.0000 Buried
22 T N -0.5700 Soluble
23 C N 0.0000 Buried
24 G N -0.6725 Soluble
25 G N -0.8910 Soluble
26 N N -2.1342 Soluble
27 N N -2.8015 Soluble
28 I N 0.0000 Buried
29 G N -1.9151 Soluble
36 S N -1.3883 Soluble
37 K N -1.1690 Soluble
38 S N -0.2050 Soluble
39 V N 0.0000 Buried
40 H N 0.0000 Buried
41 W N 0.0000 Buried
42 Y N 0.0000 Buried
43 Q N 0.0000 Buried
44 Q N 0.0000 Buried
45 K N -1.3101 Soluble
46 P N -1.1859 Soluble
47 G N -1.4689 Soluble
48 Q N -1.7338 Soluble
49 A N -0.7116 Soluble
50 P N 0.0000 Buried
51 V N 1.3447 Aggregation-prone
52 L N 0.0000 Buried
53 V N 0.0000 Buried
54 I N 0.0000 Buried
55 Y N -0.2339 Soluble
56 Y N -0.0929 Soluble
57 D N -0.8064 Soluble
65 S N -1.0389 Soluble
66 D N -1.4220 Soluble
67 R N -1.5659 Soluble
68 P N -1.0174 Soluble
69 S N -0.8446 Soluble
70 G N -0.7959 Soluble
71 I N -0.5624 Soluble
72 P N -1.3021 Soluble
74 E N -2.2520 Soluble
75 R N -1.4880 Soluble
76 F N 0.0000 Buried
77 S N -1.2453 Soluble
78 G N -0.8855 Soluble
79 S N -0.5239 Soluble
80 N N -1.0183 Soluble
83 S N -1.3872 Soluble
84 G N -2.0033 Soluble
85 N N -2.6938 Soluble
86 T N -1.4130 Soluble
87 A N 0.0000 Buried
88 T N -0.7686 Soluble
89 L N 0.0000 Buried
90 T N -0.9789 Soluble
91 I N 0.0000 Buried
92 S N -1.6696 Soluble
93 G N -1.7781 Soluble
94 V N 0.0000 Buried
95 E N -2.4730 Soluble
96 A N -1.1688 Soluble
97 G N -1.2065 Soluble
98 D N 0.0000 Buried
99 E N -1.2646 Soluble
100 A N 0.0000 Buried
101 D N 0.0000 Buried
102 Y N 0.0000 Buried
103 Y N 0.0000 Buried
104 C N 0.0000 Buried
105 Q N 0.0000 Buried
106 V N 0.0000 Buried
107 W N 0.0095 Aggregation-prone
108 D N -0.2177 Soluble
109 S N 0.2235 Aggregation-prone
110 I N 1.4184 Aggregation-prone
113 S N 0.1669 Aggregation-prone
114 D N -0.5861 Soluble
115 H N -0.4975 Soluble
116 R N 0.0000 Buried
117 V N 0.1738 Aggregation-prone
118 F N 0.0000 Buried
119 G N 0.0000 Buried
120 G N -0.7685 Soluble
121 G N 0.0000 Buried
122 T N 0.0000 Buried
123 K N -1.9756 Soluble
124 L N 0.0000 Buried
125 T N -0.4842 Soluble
126 V N 0.0000 Buried
127 L N 0.5724 Aggregation-prone
128 G N -0.7343 Soluble
129 Q N -1.7222 Soluble
130 P N -1.5797 Soluble
131 K N -2.3552 Soluble
132 A N -1.3972 Soluble
133 A N -0.8007 Soluble
134 P N 0.0000 Buried
135 S N -0.2935 Soluble
136 V N 0.0000 Buried
137 T N -0.2778 Soluble
138 L N -0.2476 Soluble
139 F N 0.0000 Buried
140 P N -0.0718 Soluble
141 P N 0.0000 Buried
142 S N -0.7808 Soluble
143 S N -1.3201 Soluble
144 E N -2.0883 Soluble
145 E N 0.0000 Buried
146 L N -1.7264 Soluble
147 Q N -2.1090 Soluble
148 A N -1.5981 Soluble
149 N N -2.3159 Soluble
150 K N -1.9132 Soluble
151 A N 0.0000 Buried
152 T N 0.0000 Buried
153 L N 0.0000 Buried
154 V N 0.0000 Buried
155 C N 0.0000 Buried
156 L N 0.0000 Buried
157 I N 0.0000 Buried
158 S N -0.6527 Soluble
159 D N -1.3643 Soluble
160 F N 0.0000 Buried
161 Y N -1.4113 Soluble
162 P N -1.2473 Soluble
163 G N -0.7175 Soluble
164 A N -0.2234 Soluble
165 V N -0.1316 Soluble
166 T N -0.0661 Soluble
167 V N -0.1643 Soluble
168 A N -0.4395 Soluble
169 W N 0.0000 Buried
170 K N -0.9679 Soluble
171 A N 0.0000 Buried
172 D N -1.3545 Soluble
173 S N -0.8376 Soluble
174 S N -0.4876 Soluble
175 P N -0.7178 Soluble
176 V N -0.5784 Soluble
177 K N -1.5524 Soluble
178 A N -0.7785 Soluble
179 G N -0.6709 Soluble
180 V N -0.5284 Soluble
181 E N -0.2738 Soluble
182 T N 0.0801 Aggregation-prone
183 T N 0.0515 Aggregation-prone
184 T N -0.1199 Soluble
185 P N -0.4318 Soluble
186 S N -1.0382 Soluble
187 K N -2.0912 Soluble
188 Q N -1.8057 Soluble
189 S N -1.5074 Soluble
190 N N -2.0365 Soluble
191 N N -2.5337 Soluble
192 K N -2.1860 Soluble
193 Y N -1.2715 Soluble
194 A N 0.0000 Buried
195 A N 0.0000 Buried
196 S N 0.0000 Buried
197 S N 0.0000 Buried
198 Y N 0.0000 Buried
199 L N 0.0000 Buried
200 S N -0.5532 Soluble
201 L N 0.0000 Buried
202 T N -1.6637 Soluble
203 P N -2.2761 Soluble
204 E N -3.1235 Soluble
205 Q N -2.3578 Soluble
206 W N 0.0000 Buried
207 K N -3.2056 Soluble
208 S N -2.4313 Soluble
209 H N -2.5098 Soluble
210 R N -2.5816 Soluble
211 S N 0.0000 Buried
212 Y N 0.0000 Buried
213 S N 0.0000 Buried
214 C N 0.0000 Buried
215 Q N -0.8441 Soluble
216 V N 0.0000 Buried
217 T N -0.6235 Soluble
218 H N 0.0000 Buried
219 E N -2.2820 Soluble
220 G N -1.4382 Soluble
221 S N -0.8567 Soluble
222 T N -0.5861 Soluble
223 V N -0.4325 Soluble
224 E N -1.5912 Soluble
225 K N -1.3666 Soluble
226 T N -0.7862 Soluble
227 V N -0.3150 Soluble
228 A N -0.8621 Soluble
229 P N -1.1099 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.4732 2.4889 CSV PDB
4.5 -0.5051 2.4587 CSV PDB
5.0 -0.5426 2.4253 CSV PDB
5.5 -0.5771 2.3907 CSV PDB
6.0 -0.5986 2.3558 CSV PDB
6.5 -0.5998 2.3208 CSV PDB
7.0 -0.5812 2.286 CSV PDB
7.5 -0.5496 2.2518 CSV PDB
8.0 -0.5114 2.2196 CSV PDB
8.5 -0.4688 2.1922 CSV PDB
9.0 -0.4224 2.1732 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH