Crenezumab / 5vzx

A4DAB074_5vzx_E_I | Source PDB: 5vzx | Chains: E, I | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7397
Max Score
1.0462
Min Score
-3.615
Total Score
-320.28
Score CDR
-0.3817
Score non-CDR
-0.7802

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYGMSWVRQAPGKGLELVASINSNGGSTYYPDSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCASGDYWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIVMTQSPLSLPVTPGEPASISCRSSQSLVYSNGDTYLHWYLQKPGQSPQLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCSQSTHVPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) E (Heavy), I (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.615
Maximum Score 1.0462
Average Score -0.7397
Total Score -320.2787

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E E -1.9937 Soluble
2 V E -1.1126 Soluble
3 Q E -1.2382 Soluble
4 L E 0.0000 Buried
5 V E 0.5518 Aggregation-prone
6 E E 0.0000 Buried
7 S E -0.4819 Soluble
8 G E -0.9198 Soluble
9 G E -0.5413 Soluble
11 G E -0.5552 Soluble
12 L E -0.1802 Soluble
13 V E 0.0000 Buried
14 Q E -1.6549 Soluble
15 P E -1.7307 Soluble
16 G E -1.4416 Soluble
17 G E -1.1444 Soluble
18 S E -1.2683 Soluble
19 L E -1.1402 Soluble
20 R E -1.9954 Soluble
21 L E 0.0000 Buried
22 S E -0.4972 Soluble
23 C E 0.0000 Buried
24 A E -0.3109 Soluble
25 A E 0.0000 Buried
26 S E -1.0500 Soluble
27 G E -1.1194 Soluble
28 F E -0.3708 Soluble
29 T E -0.1023 Soluble
30 F E 0.0000 Buried
35 S E -0.9349 Soluble
36 S E -0.3916 Soluble
37 Y E -0.1312 Soluble
38 G E -0.6440 Soluble
39 M E 0.0000 Buried
40 S E 0.0000 Buried
41 W E 0.0000 Buried
42 V E 0.0000 Buried
43 R E 0.0000 Buried
44 Q E -0.9153 Soluble
45 A E -1.1948 Soluble
46 P E -1.0329 Soluble
47 G E -1.4658 Soluble
48 K E -2.4255 Soluble
49 G E -1.6608 Soluble
50 L E -1.0772 Soluble
51 E E -1.4595 Soluble
52 L E 0.0000 Buried
53 V E 0.0000 Buried
54 A E 0.0000 Buried
55 S E 0.1479 Aggregation-prone
56 I E 0.0000 Buried
57 N E -1.1770 Soluble
58 S E -1.2017 Soluble
59 N E -1.9193 Soluble
62 G E -1.6859 Soluble
63 G E -1.2509 Soluble
64 S E -0.8788 Soluble
65 T E -0.0606 Soluble
66 Y E 0.1737 Aggregation-prone
67 Y E -0.6730 Soluble
68 P E -1.6722 Soluble
69 D E -2.5215 Soluble
70 S E -1.7121 Soluble
71 V E 0.0000 Buried
72 K E -2.5551 Soluble
74 G E -1.7128 Soluble
75 R E 0.0000 Buried
76 F E 0.0000 Buried
77 T E -0.8103 Soluble
78 I E 0.0000 Buried
79 S E -0.5693 Soluble
80 R E -1.3228 Soluble
81 D E -1.6554 Soluble
82 N E -1.5990 Soluble
83 A E -1.3416 Soluble
84 K E -2.2678 Soluble
85 N E -1.7382 Soluble
86 S E 0.0000 Buried
87 L E 0.0000 Buried
88 Y E 0.0000 Buried
89 L E 0.0000 Buried
90 Q E -1.2248 Soluble
91 M E 0.0000 Buried
92 N E -1.4392 Soluble
93 S E -1.2619 Soluble
94 L E 0.0000 Buried
95 R E -2.2900 Soluble
96 A E -1.7322 Soluble
97 E E -2.2291 Soluble
98 D E 0.0000 Buried
99 T E -0.7670 Soluble
100 A E 0.0000 Buried
101 V E 0.0629 Aggregation-prone
102 Y E 0.0000 Buried
103 Y E 0.0000 Buried
104 C E 0.0000 Buried
105 A E 0.0000 Buried
106 S E -0.0272 Soluble
107 G E -0.2301 Soluble
116 D E -0.2760 Soluble
117 Y E -0.3082 Soluble
118 W E 0.0000 Buried
119 G E 0.0000 Buried
120 Q E -1.3281 Soluble
121 G E 0.0000 Buried
122 T E -0.1999 Soluble
123 T E -0.2285 Soluble
124 V E 0.0000 Buried
125 T E 0.0000 Buried
126 V E 0.0000 Buried
127 S E -0.9864 Soluble
128 S E -0.6333 Soluble
129 A E -0.5363 Soluble
130 S E -0.6880 Soluble
131 T E -0.8163 Soluble
132 K E -1.7021 Soluble
133 G E -1.7695 Soluble
134 P E 0.0000 Buried
135 S E -0.5561 Soluble
136 V E 0.0000 Buried
137 F E 0.0000 Buried
138 P E -1.0743 Soluble
139 L E 0.0000 Buried
140 A E -1.0044 Soluble
141 P E 0.0000 Buried
142 S E -1.1496 Soluble
143 S E -1.3329 Soluble
144 K E -1.8945 Soluble
145 S E -1.1837 Soluble
146 T E -1.1082 Soluble
147 S E -0.7946 Soluble
148 G E -0.8755 Soluble
149 G E -0.9275 Soluble
150 T E -0.6358 Soluble
151 A E 0.0000 Buried
152 A E 0.0000 Buried
153 L E 0.0000 Buried
154 G E 0.0000 Buried
155 C E 0.0000 Buried
156 L E 0.0000 Buried
157 V E 0.0000 Buried
158 K E 0.0000 Buried
159 D E -0.7161 Soluble
160 Y E 0.0000 Buried
161 F E 0.0000 Buried
162 P E 0.0000 Buried
163 E E -0.6046 Soluble
164 P E -0.6604 Soluble
165 V E -0.6913 Soluble
166 T E -0.6470 Soluble
167 V E -0.1643 Soluble
168 S E -0.3348 Soluble
169 W E 0.0000 Buried
170 N E -0.6920 Soluble
171 S E -0.5930 Soluble
172 G E -0.5126 Soluble
173 A E -0.2365 Soluble
174 L E 0.0198 Aggregation-prone
175 T E -0.1449 Soluble
176 S E -0.1409 Soluble
177 G E -0.1571 Soluble
178 V E 0.1879 Aggregation-prone
179 H E -0.1362 Soluble
180 T E 0.0620 Aggregation-prone
181 F E 0.0000 Buried
182 P E -0.3340 Soluble
183 A E 0.2368 Aggregation-prone
184 V E 0.4539 Aggregation-prone
185 L E 1.0462 Aggregation-prone
186 Q E 0.1789 Aggregation-prone
187 S E -0.2565 Soluble
188 S E -0.2796 Soluble
189 G E -0.0148 Soluble
190 L E -0.0954 Soluble
191 Y E 0.2817 Aggregation-prone
192 S E 0.0000 Buried
193 L E 0.0000 Buried
194 S E 0.0000 Buried
195 S E 0.0000 Buried
196 V E 0.0000 Buried
197 V E 0.0000 Buried
198 T E -0.1395 Soluble
199 V E 0.0000 Buried
200 P E -0.5820 Soluble
201 S E -0.8574 Soluble
202 S E -0.5035 Soluble
203 S E -0.5860 Soluble
204 L E -0.7713 Soluble
205 G E -1.0266 Soluble
206 T E -0.8874 Soluble
207 Q E -1.3556 Soluble
208 T E -1.2264 Soluble
209 Y E 0.0000 Buried
210 I E -1.1143 Soluble
211 C E 0.0000 Buried
212 N E -1.3366 Soluble
213 V E 0.0000 Buried
214 N E -2.1057 Soluble
215 H E 0.0000 Buried
216 K E -2.7980 Soluble
217 P E -1.6729 Soluble
218 S E -2.0182 Soluble
219 N E -2.7101 Soluble
220 T E -2.2554 Soluble
221 K E -2.7711 Soluble
222 V E -1.5789 Soluble
223 D E -2.1711 Soluble
224 K E -2.0788 Soluble
225 K E -2.4204 Soluble
226 V E 0.0000 Buried
227 E E -2.5303 Soluble
228 P E -1.7551 Soluble
229 K E -2.2060 Soluble
230 S E -1.1073 Soluble
1 D I -1.2430 Soluble
2 I I 0.0000 Buried
3 V I 0.7767 Aggregation-prone
4 M I 0.0000 Buried
5 T I -0.3746 Soluble
6 Q I 0.0000 Buried
7 S I -0.3254 Soluble
8 P I 0.0079 Aggregation-prone
9 L I 0.3010 Aggregation-prone
10 S I -0.1551 Soluble
11 L I -0.2751 Soluble
12 P I -0.6301 Soluble
13 V I 0.0000 Buried
14 T I -1.5447 Soluble
15 P I -1.8894 Soluble
16 G I -2.1813 Soluble
17 E I -2.7391 Soluble
18 P I -2.3804 Soluble
19 A I 0.0000 Buried
20 S I -0.9243 Soluble
21 I I 0.0000 Buried
22 S I -0.8515 Soluble
23 C I 0.0000 Buried
24 R I -1.5660 Soluble
25 S I 0.0000 Buried
26 S I -0.6686 Soluble
27 Q I -1.0732 Soluble
28 S I -0.4943 Soluble
29 L I 0.0000 Buried
30 V I 0.6155 Aggregation-prone
31 Y I 0.4768 Aggregation-prone
32 S I -0.3928 Soluble
34 N I -1.3178 Soluble
35 G I -0.8405 Soluble
36 D I -0.7364 Soluble
37 T I 0.0018 Aggregation-prone
38 Y I 0.1707 Aggregation-prone
39 L I 0.0000 Buried
40 H I 0.0000 Buried
41 W I 0.0000 Buried
42 Y I 0.0000 Buried
43 L I 0.0000 Buried
44 Q I -1.1825 Soluble
45 K I -1.9756 Soluble
46 P I -1.6925 Soluble
47 G I -1.7012 Soluble
48 Q I -2.1118 Soluble
49 S I -1.4604 Soluble
50 P I 0.0000 Buried
51 Q I -1.2844 Soluble
52 L I 0.0000 Buried
53 L I 0.0000 Buried
54 I I 0.0000 Buried
55 Y I -0.1972 Soluble
56 K I -0.5127 Soluble
57 V I -0.5182 Soluble
65 S I -0.8071 Soluble
66 N I -1.2235 Soluble
67 R I -1.8248 Soluble
68 F I -0.8280 Soluble
69 S I -0.6903 Soluble
70 G I -0.8341 Soluble
71 V I -0.9823 Soluble
72 P I -1.3383 Soluble
74 D I -2.5096 Soluble
75 R I -2.5133 Soluble
76 F I 0.0000 Buried
77 S I -1.6745 Soluble
78 G I -1.0646 Soluble
79 S I -0.9627 Soluble
80 G I -0.8915 Soluble
83 S I -0.6779 Soluble
84 G I -0.5512 Soluble
85 T I -1.2384 Soluble
86 D I -1.8603 Soluble
87 F I 0.0000 Buried
88 T I -1.0750 Soluble
89 L I 0.0000 Buried
90 K I -2.2596 Soluble
91 I I 0.0000 Buried
92 S I -2.6487 Soluble
93 R I -3.4981 Soluble
94 V I 0.0000 Buried
95 E I -2.8025 Soluble
96 A I -2.5440 Soluble
97 E I -2.7483 Soluble
98 D I 0.0000 Buried
99 V I 0.0000 Buried
100 G I 0.0000 Buried
101 V I -0.4864 Soluble
102 Y I 0.0000 Buried
103 Y I 0.0000 Buried
104 C I 0.0000 Buried
105 S I 0.0000 Buried
106 Q I 0.0000 Buried
107 S I 0.1250 Aggregation-prone
108 T I 0.0000 Buried
109 H I -0.1095 Soluble
114 V I 1.0285 Aggregation-prone
115 P I 0.0000 Buried
116 W I 0.2717 Aggregation-prone
117 T I 0.0276 Aggregation-prone
118 F I 0.0500 Aggregation-prone
119 G I 0.0000 Buried
120 Q I -1.2734 Soluble
121 G I 0.0000 Buried
122 T I 0.0000 Buried
123 K I -1.0602 Soluble
124 V I 0.0000 Buried
125 E I -0.9524 Soluble
126 I I 0.0000 Buried
127 K I -1.0838 Soluble
128 R I -0.7919 Soluble
129 T I 0.1068 Aggregation-prone
130 V I 0.6656 Aggregation-prone
131 A I 0.1486 Aggregation-prone
132 A I -0.0237 Soluble
133 P I 0.0000 Buried
134 S I -0.2317 Soluble
135 V I 0.0000 Buried
136 F I 0.0000 Buried
137 I I -0.0186 Soluble
138 F I 0.0000 Buried
139 P I -0.4894 Soluble
140 P I -0.8451 Soluble
141 S I -1.5658 Soluble
142 D I -2.7934 Soluble
143 E I -2.7112 Soluble
144 Q I 0.0000 Buried
145 L I -2.1771 Soluble
146 K I -2.6914 Soluble
147 S I -1.6900 Soluble
148 G I -1.3505 Soluble
149 T I -0.8813 Soluble
150 A I 0.0000 Buried
151 S I 0.0000 Buried
152 V I 0.0000 Buried
153 V I 0.0000 Buried
154 C I 0.0000 Buried
155 L I 0.0000 Buried
156 L I 0.0000 Buried
157 N I 0.0000 Buried
158 N I -0.7612 Soluble
159 F I 0.0000 Buried
160 Y I 0.0000 Buried
161 P I -1.2485 Soluble
162 R I -2.2942 Soluble
163 E I -2.9045 Soluble
164 A I -2.1787 Soluble
165 K I -2.3774 Soluble
166 V I -1.1342 Soluble
167 Q I -0.6624 Soluble
168 W I 0.0000 Buried
169 K I -0.5262 Soluble
170 V I 0.0000 Buried
171 D I -1.6375 Soluble
172 N I -1.2221 Soluble
173 A I -0.2465 Soluble
174 L I 0.5442 Aggregation-prone
175 Q I -0.3999 Soluble
176 S I -0.7283 Soluble
177 G I -1.3019 Soluble
178 N I -1.6577 Soluble
179 S I -1.5348 Soluble
180 Q I -1.5442 Soluble
181 E I -1.8441 Soluble
182 S I -0.8571 Soluble
183 V I -0.4450 Soluble
184 T I -0.9432 Soluble
185 E I -2.0814 Soluble
186 Q I 0.0000 Buried
187 D I -2.1740 Soluble
188 S I -2.5370 Soluble
189 K I -2.8826 Soluble
190 D I -2.2058 Soluble
191 S I 0.0000 Buried
192 T I 0.0000 Buried
193 Y I 0.0000 Buried
194 S I 0.0000 Buried
195 L I 0.0000 Buried
196 S I 0.0000 Buried
197 S I 0.0000 Buried
198 T I -0.7793 Soluble
199 L I 0.0000 Buried
200 T I -0.6892 Soluble
201 L I -0.6906 Soluble
202 S I -1.1746 Soluble
203 K I -2.1178 Soluble
204 A I -1.9939 Soluble
205 D I -2.7337 Soluble
206 Y I 0.0000 Buried
207 E I -3.5093 Soluble
208 K I -3.6150 Soluble
209 H I -3.0186 Soluble
210 K I -3.1452 Soluble
211 V I -1.4243 Soluble
212 Y I 0.0000 Buried
213 A I 0.0000 Buried
214 C I 0.0000 Buried
215 E I -0.7139 Soluble
216 V I 0.0000 Buried
217 T I -1.2354 Soluble
218 H I 0.0000 Buried
219 Q I -1.6423 Soluble
220 G I -0.3598 Soluble
221 L I -0.1953 Soluble
222 S I -0.4559 Soluble
223 S I -0.4298 Soluble
224 P I -0.5789 Soluble
225 V I 0.0031 Aggregation-prone
226 T I -0.4109 Soluble
227 K I -0.5568 Soluble
228 S I -0.4839 Soluble
229 F I -0.8066 Soluble
230 N I -1.8757 Soluble
231 R I -2.6112 Soluble
232 G I -2.0550 Soluble
233 E I -2.1910 Soluble
234 C I -0.9670 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7539 1.4425 CSV PDB
4.5 -0.7968 1.3697 CSV PDB
5.0 -0.8475 1.2736 CSV PDB
5.5 -0.8955 1.2164 CSV PDB
6.0 -0.9284 1.2164 CSV PDB
6.5 -0.9374 1.2164 CSV PDB
7.0 -0.9228 1.2164 CSV PDB
7.5 -0.8927 1.2846 CSV PDB
8.0 -0.8537 1.389 CSV PDB
8.5 -0.8083 1.5466 CSV PDB
9.0 -0.7569 1.7049 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH