Glofitamab / 3pp3

A4DAB132_3pp3_H_L | Source PDB: 3pp3 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7144
Max Score
1.2993
Min Score
-3.6721
Total Score
-313.62
Score CDR
—
Score non-CDR
—

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGSSVKVSCKASGYAFSYSWINWVRQAPGQGLEWMGRIFPGDGDTDYNGKFKGRVTITADKSTSTAYMELSSLRSEDTAVYYCARNVFDGYWLVYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIVMTQTPLSLPVTPGEPASISCRSSKSLLHSNGITYLYWYLQKPGQSPQLLIYQMSNLVSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCAQNLELPYTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6721
Maximum Score 1.2993
Average Score -0.7144
Total Score -313.622

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.5312 Soluble
2 V H -1.0001 Soluble
3 Q H -1.3278 Soluble
4 L H 0.0000 Buried
5 V H 0.4708 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.4143 Soluble
8 G H -0.4096 Soluble
9 A H 0.2028 Aggregation-prone
11 E H -0.0294 Soluble
12 V H 0.5332 Aggregation-prone
13 K H -1.1093 Soluble
14 K H -2.1510 Soluble
15 P H -2.1763 Soluble
16 G H -1.7221 Soluble
17 S H -1.3557 Soluble
18 S H -1.3910 Soluble
19 V H 0.0000 Buried
20 K H -1.9652 Soluble
21 V H 0.0000 Buried
22 S H -0.4572 Soluble
23 C H 0.0000 Buried
24 K H -0.6796 Soluble
25 A H 0.0000 Buried
26 S H -0.8371 Soluble
27 G H -1.0222 Soluble
28 Y H -0.0978 Soluble
29 A H 0.3881 Aggregation-prone
30 F H 0.0000 Buried
35 S H -0.3254 Soluble
36 Y H 1.2993 Aggregation-prone
37 S H 0.0000 Buried
38 W H 0.3241 Aggregation-prone
39 I H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.5075 Soluble
45 A H -0.9625 Soluble
46 P H -0.9719 Soluble
47 G H -1.2290 Soluble
48 Q H -1.7451 Soluble
49 G H -1.0871 Soluble
50 L H 0.0000 Buried
51 E H -0.6068 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 R H -0.6517 Soluble
56 I H 0.0000 Buried
57 F H 0.0000 Buried
58 P H 0.0000 Buried
59 G H -1.5885 Soluble
62 D H -2.7691 Soluble
63 G H -2.6086 Soluble
64 D H -2.7879 Soluble
65 T H -1.4385 Soluble
66 D H -0.6278 Soluble
67 Y H -0.4094 Soluble
68 N H -1.0233 Soluble
69 G H -1.7796 Soluble
70 K H -2.3971 Soluble
71 F H 0.0000 Buried
72 K H -2.6596 Soluble
74 G H -1.7548 Soluble
75 R H -1.5151 Soluble
76 V H 0.0000 Buried
77 T H -0.5843 Soluble
78 I H 0.0000 Buried
79 T H -0.6728 Soluble
80 A H -1.3424 Soluble
81 D H -1.7074 Soluble
82 K H -2.4507 Soluble
83 S H -1.2783 Soluble
84 T H -1.0777 Soluble
85 S H -1.1845 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.4562 Soluble
89 M H 0.0000 Buried
90 E H -1.3144 Soluble
91 L H 0.0000 Buried
92 S H -1.1610 Soluble
93 S H -1.2378 Soluble
94 L H 0.0000 Buried
95 R H -3.1380 Soluble
96 S H -2.3919 Soluble
97 E H -2.5748 Soluble
98 D H 0.0000 Buried
99 T H -0.8172 Soluble
100 A H 0.0000 Buried
101 V H 0.4541 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.2499 Aggregation-prone
107 N H 0.6619 Aggregation-prone
108 V H 1.1902 Aggregation-prone
109 F H 1.0872 Aggregation-prone
110 D H -0.9209 Soluble
112 G H -0.1739 Soluble
113 Y H 0.4471 Aggregation-prone
114 W H 0.0000 Buried
115 L H 0.0000 Buried
116 V H 0.5155 Aggregation-prone
117 Y H 0.3771 Aggregation-prone
118 W H -0.0702 Soluble
119 G H 0.0000 Buried
120 Q H -1.1208 Soluble
121 G H -0.3302 Soluble
122 T H 0.0000 Buried
123 L H 0.8144 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.3834 Soluble
126 V H 0.0000 Buried
127 S H -1.2640 Soluble
128 S H -1.1015 Soluble
129 A H -0.5923 Soluble
130 S H -0.6114 Soluble
131 T H -0.7772 Soluble
132 K H -1.3007 Soluble
133 G H -1.4105 Soluble
134 P H -0.6680 Soluble
135 S H -0.3055 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1050 Soluble
139 L H 0.0000 Buried
140 A H -1.0896 Soluble
141 P H 0.0000 Buried
142 S H -1.3014 Soluble
143 S H -1.4253 Soluble
144 K H -2.1465 Soluble
145 S H -1.3112 Soluble
146 T H -0.8896 Soluble
147 S H -0.9545 Soluble
148 G H -0.8946 Soluble
149 G H -1.1310 Soluble
150 T H -0.6598 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.3378 Soluble
159 D H -0.5731 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.7861 Soluble
164 P H -1.0103 Soluble
165 V H -0.8118 Soluble
166 T H -0.5973 Soluble
167 V H -0.3159 Soluble
168 S H -0.3721 Soluble
169 W H 0.0000 Buried
170 N H -0.7828 Soluble
171 S H -0.6666 Soluble
172 G H -0.5632 Soluble
173 A H -0.2598 Soluble
174 L H -0.0461 Soluble
175 T H -0.2055 Soluble
176 S H -0.2355 Soluble
177 G H -0.2261 Soluble
178 V H 0.1446 Aggregation-prone
179 H H -0.3423 Soluble
180 T H 0.0137 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.2992 Soluble
183 A H 0.0636 Aggregation-prone
184 V H 0.3982 Aggregation-prone
185 L H 0.7570 Aggregation-prone
186 Q H -0.5690 Soluble
187 S H -0.5049 Soluble
188 S H -0.4021 Soluble
189 G H -0.3052 Soluble
190 L H -0.2731 Soluble
191 Y H 0.0000 Buried
192 S H 0.0005 Aggregation-prone
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1794 Soluble
199 V H 0.0000 Buried
200 P H -0.5037 Soluble
201 S H -0.5365 Soluble
202 S H -0.5463 Soluble
203 S H -0.5118 Soluble
204 L H -0.6950 Soluble
205 G H -0.8719 Soluble
206 T H -0.6423 Soluble
207 Q H -1.0986 Soluble
208 T H -0.9759 Soluble
209 Y H 0.0000 Buried
210 I H -1.0539 Soluble
211 C H 0.0000 Buried
212 N H -1.3731 Soluble
213 V H 0.0000 Buried
214 N H -1.7841 Soluble
215 H H 0.0000 Buried
216 K H -2.7686 Soluble
217 P H -1.7278 Soluble
218 S H -1.8712 Soluble
219 N H -2.6021 Soluble
220 T H -2.0696 Soluble
221 K H -2.6240 Soluble
222 V H -1.5183 Soluble
223 D H -2.3867 Soluble
224 K H -1.9876 Soluble
225 K H -2.1090 Soluble
226 V H 0.0000 Buried
227 E H -2.6694 Soluble
228 P H -2.0131 Soluble
229 K H -2.3039 Soluble
1 D L -1.5758 Soluble
2 I L 0.0000 Buried
3 V L 0.5206 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.4017 Soluble
6 Q L 0.0000 Buried
7 T L -0.1007 Soluble
8 P L 0.3542 Aggregation-prone
9 L L 1.0984 Aggregation-prone
10 S L 0.1720 Aggregation-prone
11 L L -0.0660 Soluble
12 P L -0.7599 Soluble
13 V L 0.0000 Buried
14 T L -1.6311 Soluble
15 P L -1.9936 Soluble
16 G L -2.1184 Soluble
17 E L -2.9238 Soluble
18 P L -2.3875 Soluble
19 A L -1.3884 Soluble
20 S L -0.6942 Soluble
21 I L 0.0000 Buried
22 S L -0.9876 Soluble
23 C L 0.0000 Buried
24 R L -2.3360 Soluble
25 S L 0.0000 Buried
26 S L -1.2850 Soluble
27 K L -2.0443 Soluble
28 S L -1.1958 Soluble
29 L L 0.0000 Buried
30 L L 0.4108 Aggregation-prone
31 H L -0.5843 Soluble
32 S L -0.9643 Soluble
34 N L -1.4068 Soluble
35 G L -0.6851 Soluble
36 I L 0.3103 Aggregation-prone
37 T L 0.0000 Buried
38 Y L 0.3638 Aggregation-prone
39 L L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 L L 0.0000 Buried
44 Q L -1.1772 Soluble
45 K L -1.8302 Soluble
46 P L -1.5329 Soluble
47 G L -1.3742 Soluble
48 Q L -2.1371 Soluble
49 S L -1.3665 Soluble
50 P L 0.0000 Buried
51 Q L -0.9569 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.1916 Aggregation-prone
56 Q L 0.0947 Aggregation-prone
57 M L 0.1450 Aggregation-prone
65 S L -0.2676 Soluble
66 N L -0.3178 Soluble
67 L L -0.1671 Soluble
68 V L -0.1352 Soluble
69 S L -0.4900 Soluble
70 G L -0.7290 Soluble
71 V L 0.0000 Buried
72 P L -1.1903 Soluble
74 D L -2.0534 Soluble
75 R L -2.1698 Soluble
76 F L 0.0000 Buried
77 S L -1.2099 Soluble
78 G L -0.6453 Soluble
79 S L -0.8221 Soluble
80 G L -0.9156 Soluble
83 S L -0.8272 Soluble
84 G L -0.8975 Soluble
85 T L -1.5760 Soluble
86 D L -1.9557 Soluble
87 F L 0.0000 Buried
88 T L -1.0387 Soluble
89 L L 0.0000 Buried
90 K L -1.8692 Soluble
91 I L 0.0000 Buried
92 S L -2.4254 Soluble
93 R L -3.2940 Soluble
94 V L 0.0000 Buried
95 E L -2.3769 Soluble
96 A L -1.3278 Soluble
97 E L -2.2270 Soluble
98 D L 0.0000 Buried
99 V L -0.8504 Soluble
100 G L 0.0000 Buried
101 V L -0.3440 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 A L 0.0000 Buried
106 Q L 0.0000 Buried
107 N L -0.2053 Soluble
108 L L -0.1968 Soluble
109 E L -1.1224 Soluble
114 L L -0.0263 Soluble
115 P L -0.4758 Soluble
116 Y L 0.0000 Buried
117 T L -0.0406 Soluble
118 F L 0.2512 Aggregation-prone
119 G L 0.0000 Buried
120 G L -0.3887 Soluble
121 G L -0.3409 Soluble
122 T L 0.0000 Buried
123 K L -0.7284 Soluble
124 V L 0.0000 Buried
125 E L -1.0280 Soluble
126 I L -1.0177 Soluble
127 K L -1.2235 Soluble
128 R L -0.9604 Soluble
129 T L -0.1259 Soluble
130 V L 0.1827 Aggregation-prone
131 A L -0.1305 Soluble
132 A L -0.1048 Soluble
133 P L 0.0000 Buried
134 S L -0.1657 Soluble
135 V L 0.0000 Buried
136 F L 0.1585 Aggregation-prone
137 I L 0.1628 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.4777 Soluble
140 P L 0.0000 Buried
141 S L -1.6913 Soluble
142 D L -3.1367 Soluble
143 E L -3.2377 Soluble
144 Q L 0.0000 Buried
145 L L -2.1840 Soluble
146 K L -2.8949 Soluble
147 S L -1.8470 Soluble
148 G L -1.4583 Soluble
149 T L -1.2123 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9489 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7953 Soluble
162 R L -3.1882 Soluble
163 E L -3.2610 Soluble
164 A L -2.3844 Soluble
165 K L -2.2759 Soluble
166 V L -0.9342 Soluble
167 Q L -0.4503 Soluble
168 W L 0.0000 Buried
169 K L -0.4636 Soluble
170 V L 0.0000 Buried
171 D L -2.1376 Soluble
172 N L -1.4325 Soluble
173 A L -0.3122 Soluble
174 L L 0.7303 Aggregation-prone
175 Q L -0.1067 Soluble
176 S L -0.4301 Soluble
177 G L -0.9760 Soluble
178 N L -0.9041 Soluble
179 S L -1.1688 Soluble
180 Q L -1.5853 Soluble
181 E L -1.5224 Soluble
182 S L -0.7957 Soluble
183 V L -0.7278 Soluble
184 T L -1.2140 Soluble
185 E L -2.3270 Soluble
186 Q L -1.9804 Soluble
187 D L -1.9909 Soluble
188 S L -1.8947 Soluble
189 K L -2.2334 Soluble
190 D L -1.6763 Soluble
191 S L -1.6739 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6746 Soluble
199 L L 0.0000 Buried
200 T L -0.3888 Soluble
201 L L -0.6101 Soluble
202 S L -1.2078 Soluble
203 K L -1.9956 Soluble
204 A L -1.8499 Soluble
205 D L -2.3869 Soluble
206 Y L 0.0000 Buried
207 E L -3.6340 Soluble
208 K L -3.6721 Soluble
209 H L -3.0950 Soluble
210 K L -3.2095 Soluble
211 V L -1.4868 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.5364 Soluble
216 V L 0.0000 Buried
217 T L -1.1480 Soluble
218 H L 0.0000 Buried
219 Q L -1.5978 Soluble
220 G L -0.4856 Soluble
221 L L -0.2488 Soluble
222 S L -0.4459 Soluble
223 S L -0.4063 Soluble
224 P L -0.4715 Soluble
225 V L 0.1172 Aggregation-prone
226 T L -0.3188 Soluble
227 K L -0.5441 Soluble
228 S L -0.4719 Soluble
229 F L -0.8532 Soluble
230 N L -1.9794 Soluble
231 R L -2.4883 Soluble
232 G L -2.2506 Soluble
233 E L -2.1716 Soluble
234 C L -1.0475 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6978 3.8401 CSV PDB
4.5 -0.7437 3.7117 CSV PDB
5.0 -0.7981 3.5366 CSV PDB
5.5 -0.85 3.3378 CSV PDB
6.0 -0.8859 3.1301 CSV PDB
6.5 -0.8961 2.9195 CSV PDB
7.0 -0.8804 2.7082 CSV PDB
7.5 -0.8467 2.4976 CSV PDB
8.0 -0.8025 2.2899 CSV PDB
8.5 -0.7507 2.0913 CSV PDB
9.0 -0.6917 1.916 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH