Afasevikumab / 6ppg

A4DAB008_6ppg_H_L | Source PDB: 6ppg | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6785
Max Score
1.4479
Min Score
-3.6058
Total Score
-299.88
Score CDR
-0.3134
Score non-CDR
-0.7261

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSGINWSSGGIGYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTALYYCARDIGGFGEFYWNFGLWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCEIVLTQSPATLSLSPGERATLSCRASQSVRSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPPATFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEGC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6058
Maximum Score 1.4479
Average Score -0.6785
Total Score -299.8846

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.6027 Soluble
2 V H -0.1305 Soluble
3 Q H -0.6191 Soluble
4 L H 0.0000 Buried
5 V H 0.0315 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5623 Soluble
8 G H -0.7385 Soluble
9 G H -0.5488 Soluble
11 G H -0.2614 Soluble
12 L H -0.0514 Soluble
13 V H 0.0000 Buried
14 Q H -1.7424 Soluble
15 P H -1.8347 Soluble
16 G H -1.8625 Soluble
17 R H -2.5700 Soluble
18 S H -1.8182 Soluble
19 L H -1.4529 Soluble
20 R H -1.9082 Soluble
21 L H 0.0000 Buried
22 S H -0.3307 Soluble
23 C H 0.0000 Buried
24 A H -0.2681 Soluble
25 A H 0.0000 Buried
26 S H -0.7789 Soluble
27 G H -0.8477 Soluble
28 F H -0.4462 Soluble
29 T H -0.7318 Soluble
30 F H 0.0000 Buried
35 D H -1.3929 Soluble
36 D H -1.2079 Soluble
37 Y H -0.3994 Soluble
38 A H 0.0000 Buried
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7960 Soluble
45 A H -1.1446 Soluble
46 P H -0.9625 Soluble
47 G H -1.4592 Soluble
48 K H -2.2729 Soluble
49 G H -1.3607 Soluble
50 L H 0.0000 Buried
51 E H -0.9058 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 N H -0.1965 Soluble
58 W H -0.3029 Soluble
59 S H -0.5464 Soluble
62 S H -0.1693 Soluble
63 G H -0.1110 Soluble
64 G H 0.2577 Aggregation-prone
65 I H 1.4479 Aggregation-prone
66 G H 0.0000 Buried
67 Y H -0.5024 Soluble
68 A H -1.2160 Soluble
69 D H -2.6182 Soluble
70 S H -1.7727 Soluble
71 V H 0.0000 Buried
72 K H -2.5804 Soluble
74 G H -1.7374 Soluble
75 R H -1.2980 Soluble
76 F H 0.0000 Buried
77 T H -0.5220 Soluble
78 I H 0.0000 Buried
79 S H 0.0230 Aggregation-prone
80 R H -0.8593 Soluble
81 D H -1.5147 Soluble
82 N H -1.9202 Soluble
83 A H -1.5260 Soluble
84 K H -2.3385 Soluble
85 N H -2.0824 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.1401 Soluble
89 L H 0.0000 Buried
90 Q H -1.0345 Soluble
91 M H 0.0000 Buried
92 N H -1.6916 Soluble
93 S H -1.5236 Soluble
94 L H 0.0000 Buried
95 R H -2.1560 Soluble
96 A H -1.5023 Soluble
97 E H -2.1501 Soluble
98 D H 0.0000 Buried
99 T H -0.6522 Soluble
100 A H 0.0000 Buried
101 L H 0.1518 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.0216 Soluble
107 D H 0.0000 Buried
108 I H 0.6311 Aggregation-prone
109 G H 0.0000 Buried
110 G H 0.0774 Aggregation-prone
111 F H 0.9363 Aggregation-prone
111A G H 0.0000 Buried
112B E H -0.9841 Soluble
112A F H 0.0690 Aggregation-prone
112 Y H 0.3272 Aggregation-prone
113 W H 0.2685 Aggregation-prone
114 N H 0.0000 Buried
115 F H 0.0000 Buried
116 G H -0.3631 Soluble
117 L H -0.0262 Soluble
118 W H -0.6420 Soluble
119 G H 0.0000 Buried
120 R H -1.9446 Soluble
121 G H -0.8129 Soluble
122 T H -0.2572 Soluble
123 L H 0.0554 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.5591 Soluble
128 S H -0.5823 Soluble
129 A H -0.4533 Soluble
130 S H -0.6536 Soluble
131 T H -0.9152 Soluble
132 K H -1.4831 Soluble
133 G H -1.6329 Soluble
134 P H 0.0000 Buried
135 S H -0.4554 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2748 Soluble
139 L H 0.0000 Buried
140 A H -1.2753 Soluble
141 P H 0.0000 Buried
142 S H -0.9983 Soluble
143 S H -0.8051 Soluble
144 K H -0.8575 Soluble
145 S H 0.0000 Buried
146 T H -0.6391 Soluble
147 S H -0.7590 Soluble
148 G H -0.9121 Soluble
149 G H -0.9196 Soluble
150 T H -0.6335 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.5897 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.2178 Soluble
164 P H -0.6382 Soluble
165 V H 0.0000 Buried
166 T H -0.7032 Soluble
167 V H -0.0965 Soluble
168 S H -0.4250 Soluble
169 W H 0.0000 Buried
170 N H -0.8001 Soluble
171 S H -0.6811 Soluble
172 G H -0.5201 Soluble
173 A H -0.2308 Soluble
174 L H -0.0343 Soluble
175 T H -0.1872 Soluble
176 S H -0.2112 Soluble
177 G H -0.2686 Soluble
178 V H 0.1394 Aggregation-prone
179 H H -0.2145 Soluble
180 T H 0.0512 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.2516 Soluble
183 A H 0.3315 Aggregation-prone
184 V H 0.6304 Aggregation-prone
185 L H 1.3106 Aggregation-prone
186 Q H 0.2542 Aggregation-prone
187 S H -0.1292 Soluble
188 S H -0.2479 Soluble
189 G H -0.0035 Soluble
190 L H 0.0555 Aggregation-prone
191 Y H 0.4340 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1385 Soluble
199 V H 0.0000 Buried
200 P H -0.6578 Soluble
201 S H -0.6209 Soluble
202 S H -0.6186 Soluble
203 S H -0.5841 Soluble
204 L H -0.6792 Soluble
205 G H -0.9287 Soluble
206 T H -0.6675 Soluble
207 Q H -0.9533 Soluble
208 T H -1.2045 Soluble
209 Y H 0.0000 Buried
210 I H -1.4533 Soluble
211 C H 0.0000 Buried
212 N H -1.5580 Soluble
213 V H 0.0000 Buried
214 N H -2.2493 Soluble
215 H H 0.0000 Buried
216 K H -2.7959 Soluble
217 P H -1.6485 Soluble
218 S H -1.9176 Soluble
219 N H -2.5499 Soluble
220 T H -2.1447 Soluble
221 K H -2.8626 Soluble
222 V H -1.7657 Soluble
223 D H -2.7136 Soluble
224 K H -2.3183 Soluble
225 R H -2.8173 Soluble
226 V H 0.0000 Buried
227 E H -2.7934 Soluble
228 P H -2.0204 Soluble
229 K H -2.1578 Soluble
230 S H -1.2951 Soluble
231 C H -1.1667 Soluble
1 E L -1.7166 Soluble
2 I L 0.0000 Buried
3 V L 0.6084 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.6086 Soluble
6 Q L 0.0000 Buried
7 S L -0.6578 Soluble
8 P L -0.3261 Soluble
9 A L -0.2599 Soluble
10 T L -0.1912 Soluble
11 L L -0.0829 Soluble
12 S L -0.3661 Soluble
13 L L -0.8655 Soluble
14 S L -1.5282 Soluble
15 P L -1.9073 Soluble
16 G L -1.7377 Soluble
17 E L -2.3132 Soluble
18 R L -2.5388 Soluble
19 A L 0.0000 Buried
20 T L -0.5466 Soluble
21 L L 0.0000 Buried
22 S L -0.8830 Soluble
23 C L 0.0000 Buried
24 R L -2.3389 Soluble
25 A L 0.0000 Buried
26 S L -1.0620 Soluble
27 Q L -2.0876 Soluble
28 S L -2.0195 Soluble
29 V L 0.0000 Buried
36 R L -2.2726 Soluble
37 S L -1.0805 Soluble
38 Y L -0.3299 Soluble
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.4893 Soluble
46 P L -1.1084 Soluble
47 G L -1.3836 Soluble
48 Q L -1.6849 Soluble
49 A L -1.2227 Soluble
50 P L 0.0000 Buried
51 R L -1.3285 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.1880 Soluble
56 D L -0.6069 Soluble
57 A L 0.0000 Buried
65 S L -0.9510 Soluble
66 N L -1.4895 Soluble
67 R L -2.1545 Soluble
68 A L -1.2422 Soluble
69 T L -0.7694 Soluble
70 G L -0.7037 Soluble
71 I L -0.8124 Soluble
72 P L -0.5019 Soluble
74 A L -0.5183 Soluble
75 R L -0.7386 Soluble
76 F L 0.0000 Buried
77 S L -0.7932 Soluble
78 G L -0.7467 Soluble
79 S L -0.7161 Soluble
80 G L -1.2415 Soluble
83 S L -1.3196 Soluble
84 G L -1.8717 Soluble
85 T L -2.1323 Soluble
86 D L -2.4307 Soluble
87 F L 0.0000 Buried
88 T L -0.7409 Soluble
89 L L 0.0000 Buried
90 T L -0.6321 Soluble
91 I L 0.0000 Buried
92 S L -1.6673 Soluble
93 S L -1.6742 Soluble
94 L L 0.0000 Buried
95 E L -2.3158 Soluble
96 P L -2.4644 Soluble
97 E L -2.8887 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 V L -0.1440 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 R L -0.3495 Soluble
108 S L -0.8456 Soluble
109 N L -1.2165 Soluble
113 W L 0.0633 Aggregation-prone
114 P L -0.4733 Soluble
115 P L 0.0000 Buried
116 A L 0.0000 Buried
117 T L -0.0843 Soluble
118 F L 0.1338 Aggregation-prone
119 G L 0.0000 Buried
120 G L -0.6287 Soluble
121 G L -0.6110 Soluble
122 T L 0.0000 Buried
123 K L -0.5817 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L 0.0000 Buried
127 K L -1.3504 Soluble
128 R L -0.9171 Soluble
129 T L 0.1622 Aggregation-prone
130 V L 0.9040 Aggregation-prone
131 A L 0.3970 Aggregation-prone
132 A L 0.0030 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.2737 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.5059 Soluble
140 P L 0.0000 Buried
141 S L -1.4883 Soluble
142 D L -2.5149 Soluble
143 E L -2.3516 Soluble
144 Q L 0.0000 Buried
145 L L -1.8114 Soluble
146 K L -2.6145 Soluble
147 S L -1.6207 Soluble
148 G L -1.3548 Soluble
149 T L -0.8943 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7377 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3525 Soluble
162 R L -2.1608 Soluble
163 E L -2.9786 Soluble
164 A L -2.2054 Soluble
165 K L -2.2812 Soluble
166 V L -1.1139 Soluble
167 Q L -0.6621 Soluble
168 W L 0.0000 Buried
169 K L -0.6446 Soluble
170 V L 0.0000 Buried
171 D L -1.3483 Soluble
172 N L -1.2349 Soluble
173 A L -0.3900 Soluble
174 L L 0.3132 Aggregation-prone
175 Q L -0.3774 Soluble
176 S L -0.5478 Soluble
177 G L -0.9605 Soluble
178 N L -0.9696 Soluble
179 S L -1.0357 Soluble
180 Q L -1.0536 Soluble
181 E L -1.4856 Soluble
182 S L -0.4741 Soluble
183 V L -0.0612 Soluble
184 T L -0.5683 Soluble
185 E L -1.5340 Soluble
186 Q L 0.0000 Buried
187 D L -1.9724 Soluble
188 S L -2.4554 Soluble
189 K L -2.7338 Soluble
190 D L -1.8905 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5104 Soluble
199 L L 0.0000 Buried
200 T L -0.3730 Soluble
201 L L -0.6654 Soluble
202 S L -1.1925 Soluble
203 K L -2.0242 Soluble
204 A L -1.8581 Soluble
205 D L -2.4987 Soluble
206 Y L 0.0000 Buried
207 E L -3.6058 Soluble
208 K L -3.5560 Soluble
209 H L -2.9370 Soluble
210 K L -2.9595 Soluble
211 V L -1.2260 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7052 Soluble
216 V L 0.0000 Buried
217 T L -1.2227 Soluble
218 H L 0.0000 Buried
219 Q L -1.4048 Soluble
220 G L -0.2826 Soluble
221 L L -0.1876 Soluble
222 S L -0.4802 Soluble
223 S L -0.4385 Soluble
224 P L -0.5742 Soluble
225 V L -0.1151 Soluble
226 T L -0.5131 Soluble
227 K L -0.8681 Soluble
228 S L -0.5704 Soluble
229 F L 0.0000 Buried
230 N L -1.6631 Soluble
231 R L -2.0024 Soluble
232 G L -2.1437 Soluble
233 E L -2.4222 Soluble
234 G L -1.5012 Soluble
235 C L -1.0736 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6844 4.6628 CSV PDB
4.5 -0.7267 4.5093 CSV PDB
5.0 -0.7775 4.2903 CSV PDB
5.5 -0.8274 4.0347 CSV PDB
6.0 -0.8654 3.7716 CSV PDB
6.5 -0.8824 3.5256 CSV PDB
7.0 -0.8774 3.3181 CSV PDB
7.5 -0.8573 3.1524 CSV PDB
8.0 -0.8289 3.0148 CSV PDB
8.5 -0.7946 2.8961 CSV PDB
9.0 -0.7545 2.7997 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH