Renvistobart / 8szy

A4DAB224_8szy_H_L | Source PDB: 8szy | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6217
Max Score
2.2381
Min Score
-3.5864
Total Score
-272.32
Score CDR
-0.0264
Score non-CDR
-0.7144

Calculation Parameters

Chain Sequence(s) VHLQESGPGLVKPSETLSLTCTVSGGSVSSGIYYWSWIRQPPGKGLEWIGYIYYSGSTNYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARDYYVSGNYYNVDYYFFGVDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPEIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPPLFTFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5864
Maximum Score 2.2381
Average Score -0.6217
Total Score -272.3154

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.4540 Aggregation-prone
3 H H -0.9418 Soluble
4 L H 0.0000 Buried
5 Q H -1.6651 Soluble
6 E H 0.0000 Buried
7 S H -0.8031 Soluble
8 G H -0.5357 Soluble
9 P H -0.7416 Soluble
11 G H -0.5034 Soluble
12 L H -0.5365 Soluble
13 V H 0.0000 Buried
14 K H -2.4278 Soluble
15 P H -1.7301 Soluble
16 S H -1.7306 Soluble
17 E H -2.5961 Soluble
18 T H -1.6513 Soluble
19 L H 0.0000 Buried
20 S H -0.8503 Soluble
21 L H 0.0000 Buried
22 T H -0.5321 Soluble
23 C H 0.0000 Buried
24 T H -1.1519 Soluble
25 V H 0.0000 Buried
26 S H -0.6978 Soluble
27 G H -0.4559 Soluble
28 G H -0.4146 Soluble
29 S H -0.4942 Soluble
30 V H 0.0000 Buried
31 S H 0.0969 Aggregation-prone
34 S H 0.5513 Aggregation-prone
35 G H 0.9775 Aggregation-prone
36 I H 2.2381 Aggregation-prone
37 Y H 1.4254 Aggregation-prone
38 Y H 0.0000 Buried
39 W H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 I H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7504 Soluble
45 P H -0.8341 Soluble
46 P H -0.9296 Soluble
47 G H -1.4323 Soluble
48 K H -2.1981 Soluble
49 G H -1.3175 Soluble
50 L H 0.0000 Buried
51 E H -0.7955 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 Y H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H 0.4721 Aggregation-prone
58 Y H 0.6304 Aggregation-prone
59 S H -0.0357 Soluble
63 G H -0.2323 Soluble
64 S H -0.4289 Soluble
65 T H -0.5440 Soluble
66 N H -1.2795 Soluble
67 Y H -1.0161 Soluble
68 N H -1.0095 Soluble
69 P H -1.1608 Soluble
70 S H -0.8810 Soluble
71 L H 0.0000 Buried
72 K H -2.1970 Soluble
74 S H -1.3212 Soluble
75 R H -1.3206 Soluble
76 V H 0.0000 Buried
77 T H -0.9093 Soluble
78 I H 0.0000 Buried
79 S H -0.2716 Soluble
80 V H -0.3929 Soluble
81 D H -1.2383 Soluble
82 T H -1.0530 Soluble
83 S H -1.3933 Soluble
84 K H -2.2188 Soluble
85 N H -1.5596 Soluble
86 Q H -1.3488 Soluble
87 F H 0.0000 Buried
88 S H -0.4431 Soluble
89 L H 0.0000 Buried
90 K H -1.0260 Soluble
91 L H 0.0000 Buried
92 S H -1.1452 Soluble
93 S H -1.3019 Soluble
94 V H 0.0000 Buried
95 T H -0.6734 Soluble
96 A H -0.2179 Soluble
97 A H 0.0580 Aggregation-prone
98 D H 0.0000 Buried
99 T H 0.0424 Aggregation-prone
100 A H 0.0000 Buried
101 V H 0.0733 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 D H 0.0000 Buried
108 Y H 1.2489 Aggregation-prone
109 Y H 1.3682 Aggregation-prone
110 V H 0.6179 Aggregation-prone
111 S H 0.2328 Aggregation-prone
111A G H -0.5669 Soluble
111B N H -0.7294 Soluble
111C Y H 0.3889 Aggregation-prone
111D Y H 0.5496 Aggregation-prone
112E N H -0.7095 Soluble
112D V H -0.5448 Soluble
112C D H -1.4887 Soluble
112B Y H -0.5537 Soluble
112A Y H 0.3897 Aggregation-prone
112 F H 0.9295 Aggregation-prone
113 F H 0.8875 Aggregation-prone
114 G H 0.0000 Buried
115 V H 0.0000 Buried
116 D H -0.2228 Soluble
117 V H -0.0125 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.8581 Soluble
121 G H 0.0000 Buried
122 T H -0.6370 Soluble
123 T H -0.3508 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.7136 Soluble
128 S H -0.8562 Soluble
129 A H -0.5740 Soluble
130 S H -0.6780 Soluble
131 T H -0.9537 Soluble
132 K H -1.3388 Soluble
133 G H -1.5752 Soluble
134 P H 0.0000 Buried
135 S H -0.4003 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1319 Soluble
139 L H 0.0000 Buried
140 A H -0.7771 Soluble
141 P H 0.0000 Buried
142 S H -0.6187 Soluble
143 G H -0.6157 Soluble
144 T H -0.4608 Soluble
145 A H -0.2287 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.8251 Soluble
154 Y H 0.0000 Buried
155 F H -0.2477 Soluble
156 P H 0.0000 Buried
157 E H -0.4635 Soluble
158 P H -0.6480 Soluble
159 V H 0.0000 Buried
160 T H -0.7508 Soluble
161 V H 0.0000 Buried
162 S H -0.5112 Soluble
163 W H 0.0000 Buried
164 N H -0.7530 Soluble
165 S H -0.7235 Soluble
166 G H -0.6148 Soluble
167 A H -0.2649 Soluble
168 L H 0.0061 Aggregation-prone
169 T H -0.1449 Soluble
170 S H -0.1459 Soluble
171 G H -0.1317 Soluble
172 V H 0.2252 Aggregation-prone
173 H H -0.3112 Soluble
174 T H -0.1741 Soluble
175 F H 0.0000 Buried
176 P H -0.2763 Soluble
177 A H 0.4720 Aggregation-prone
178 V H 0.6675 Aggregation-prone
179 L H 1.2286 Aggregation-prone
180 Q H 0.2306 Aggregation-prone
181 S H -0.0874 Soluble
182 S H -0.3035 Soluble
183 G H -0.0221 Soluble
184 L H 0.0674 Aggregation-prone
185 Y H 0.3769 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1411 Soluble
193 V H 0.0000 Buried
194 P H -0.4288 Soluble
195 S H -0.5331 Soluble
196 S H -0.5941 Soluble
197 S H -0.5829 Soluble
198 L H -0.8605 Soluble
199 G H -1.0045 Soluble
200 T H -0.6893 Soluble
201 Q H -1.1682 Soluble
202 T H -1.1117 Soluble
203 Y H 0.0000 Buried
204 I H -1.1732 Soluble
205 C H 0.0000 Buried
206 N H -1.5774 Soluble
207 V H 0.0000 Buried
208 N H -2.1145 Soluble
209 H H 0.0000 Buried
210 K H -2.7693 Soluble
211 P H -1.5842 Soluble
212 S H -1.8613 Soluble
213 N H -2.5987 Soluble
214 T H -2.1320 Soluble
215 K H -2.7946 Soluble
216 V H -1.6985 Soluble
217 D H -2.5863 Soluble
218 K H -2.1280 Soluble
219 R H -2.3996 Soluble
220 V H 0.0000 Buried
221 E H -2.4471 Soluble
222 P H -1.2204 Soluble
1 E L -1.5158 Soluble
2 I L 0.0000 Buried
3 V L 0.7214 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.3277 Soluble
6 Q L 0.0000 Buried
7 S L -0.5795 Soluble
8 P L -0.3368 Soluble
9 A L -0.3211 Soluble
10 T L -0.4043 Soluble
11 L L -0.3487 Soluble
12 S L -0.6526 Soluble
13 L L -1.2826 Soluble
14 S L -1.6682 Soluble
15 P L -1.8685 Soluble
16 G L -1.9211 Soluble
17 E L -2.7503 Soluble
18 R L -2.8462 Soluble
19 A L 0.0000 Buried
20 T L -0.7138 Soluble
21 L L 0.0000 Buried
22 S L -0.7332 Soluble
23 C L 0.0000 Buried
24 R L -1.6737 Soluble
25 A L 0.0000 Buried
26 S L -0.8642 Soluble
27 Q L -1.5645 Soluble
28 S L -1.1241 Soluble
29 V L 0.0000 Buried
36 S L -0.5704 Soluble
37 S L -0.1939 Soluble
38 Y L 0.0046 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.3974 Soluble
46 P L -1.3064 Soluble
47 G L -1.3898 Soluble
48 Q L -2.0136 Soluble
49 A L -1.3089 Soluble
50 P L 0.0000 Buried
51 R L -1.3147 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.0267 Soluble
56 D L -0.1786 Soluble
57 A L 0.0000 Buried
65 S L -0.9568 Soluble
66 N L -1.2021 Soluble
67 R L -1.3481 Soluble
68 A L -0.7354 Soluble
69 T L -0.5340 Soluble
70 G L -0.5244 Soluble
71 I L -0.4216 Soluble
72 P L -0.3351 Soluble
74 A L -0.3570 Soluble
75 R L -0.7613 Soluble
76 F L 0.0000 Buried
77 S L -0.6689 Soluble
78 G L 0.0000 Buried
79 S L -0.8180 Soluble
80 G L -1.0678 Soluble
83 S L -1.0310 Soluble
84 G L -1.1050 Soluble
85 T L -1.5716 Soluble
86 D L -1.9969 Soluble
87 F L 0.0000 Buried
88 T L -0.7171 Soluble
89 L L 0.0000 Buried
90 T L -0.6029 Soluble
91 I L 0.0000 Buried
92 S L -1.4590 Soluble
93 S L -1.8127 Soluble
94 L L 0.0000 Buried
95 E L -2.1625 Soluble
96 P L -2.1409 Soluble
97 E L -2.3403 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 V L -0.4111 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 R L 0.0000 Buried
108 S L 0.0000 Buried
109 N L -0.9122 Soluble
110 W L -0.6022 Soluble
113 P L -0.7035 Soluble
114 P L -0.3802 Soluble
115 L L -0.1558 Soluble
116 F L 0.0000 Buried
117 T L 0.2115 Aggregation-prone
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 P L -0.7159 Soluble
121 G L -0.6217 Soluble
122 T L 0.0000 Buried
123 K L -0.9722 Soluble
124 V L 0.0000 Buried
125 D L -1.0236 Soluble
126 I L 0.0000 Buried
127 K L -1.3309 Soluble
128 R L -1.0124 Soluble
129 T L -0.1123 Soluble
130 V L 0.2251 Aggregation-prone
131 A L 0.0704 Aggregation-prone
132 A L -0.1339 Soluble
133 P L 0.0000 Buried
134 S L -0.0753 Soluble
135 V L 0.0478 Aggregation-prone
136 F L 0.2107 Aggregation-prone
137 I L 0.0742 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.8828 Soluble
140 P L -0.8037 Soluble
141 S L -1.5429 Soluble
142 D L -2.7881 Soluble
143 E L -2.7378 Soluble
144 Q L 0.0000 Buried
145 L L -2.1482 Soluble
146 K L -2.6593 Soluble
147 S L -1.7286 Soluble
148 G L -1.2734 Soluble
149 T L -0.8786 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8175 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7752 Soluble
162 R L -3.1060 Soluble
163 E L -3.2861 Soluble
164 A L -2.3638 Soluble
165 K L -2.5165 Soluble
166 V L -1.1179 Soluble
167 Q L -0.6509 Soluble
168 W L 0.0000 Buried
169 K L -0.5201 Soluble
170 V L 0.0000 Buried
171 D L -1.4259 Soluble
172 N L -1.1525 Soluble
173 A L -0.1942 Soluble
174 L L 0.7517 Aggregation-prone
175 Q L -0.4237 Soluble
176 S L -0.6847 Soluble
177 G L -1.3473 Soluble
178 N L -1.7421 Soluble
179 S L -1.5558 Soluble
180 Q L -1.5481 Soluble
181 E L -2.0667 Soluble
182 S L -0.9172 Soluble
183 V L -0.6526 Soluble
184 T L -0.9354 Soluble
185 E L -1.8828 Soluble
186 Q L 0.0000 Buried
187 D L -2.0336 Soluble
188 S L -2.3473 Soluble
189 K L -2.6945 Soluble
190 D L -1.8920 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7775 Soluble
199 L L 0.0000 Buried
200 T L -0.5364 Soluble
201 L L -0.8589 Soluble
202 S L -1.0845 Soluble
203 K L -2.0768 Soluble
204 A L -1.9070 Soluble
205 D L -2.7147 Soluble
206 Y L 0.0000 Buried
207 E L -3.3856 Soluble
208 K L -3.5864 Soluble
209 H L -3.0287 Soluble
210 K L -3.1016 Soluble
211 V L -1.6614 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9816 Soluble
216 V L 0.0000 Buried
217 T L -1.1397 Soluble
218 H L 0.0000 Buried
219 Q L -1.6116 Soluble
220 G L -0.4673 Soluble
221 L L -0.2479 Soluble
222 S L -0.4622 Soluble
223 S L -0.4449 Soluble
224 P L -0.6332 Soluble
225 V L -0.2081 Soluble
226 T L -0.6278 Soluble
227 K L -0.8892 Soluble
228 S L -0.8386 Soluble
229 F L 0.0000 Buried
230 N L -2.7603 Soluble
231 R L -2.9810 Soluble
232 G L -2.2176 Soluble
233 E L -2.6514 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6144 3.1523 CSV PDB
4.5 -0.6533 3.1523 CSV PDB
5.0 -0.6994 3.1523 CSV PDB
5.5 -0.7431 3.1523 CSV PDB
6.0 -0.7736 3.1523 CSV PDB
6.5 -0.7832 3.1523 CSV PDB
7.0 -0.772 3.1523 CSV PDB
7.5 -0.747 3.1523 CSV PDB
8.0 -0.7144 3.1522 CSV PDB
8.5 -0.6765 3.152 CSV PDB
9.0 -0.6335 3.1513 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH