Epcoritamab / 3giz

A4DAB106_3giz_H_L | Source PDB: 3giz | Chains: H, L | Done
98.25
Yes
10 Å
Yes
Avg A4D Score
-0.6894
Max Score
1.4322
Min Score
-3.5615
Total Score
-294.39
Score CDR
-0.1793
Score non-CDR
-0.7556

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGRSLRLSCAASGFTFNDYAMHWVRQAPGKGLEWVSTISWNSGSIGYADSVKGRFTISRDNAKKSLYLQMNSLRAEDTALYYCAKDIQYGNYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPEIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPITFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5615
Maximum Score 1.4322
Average Score -0.6894
Total Score -294.3853

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.8187 Soluble
2 V H -0.6668 Soluble
3 Q H -0.8149 Soluble
4 L H 0.0000 Buried
5 V H 1.1847 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4404 Soluble
8 G H -1.0129 Soluble
9 G H -0.6109 Soluble
11 G H -0.2179 Soluble
12 L H 0.0712 Aggregation-prone
13 V H -0.8289 Soluble
14 Q H -1.8522 Soluble
15 P H -2.0978 Soluble
16 G H -2.1593 Soluble
17 R H -2.6973 Soluble
18 S H -2.0948 Soluble
19 L H -1.5061 Soluble
20 R H -2.2954 Soluble
21 L H 0.0000 Buried
22 S H -0.4212 Soluble
23 C H 0.0000 Buried
24 A H -0.0828 Soluble
25 A H 0.0000 Buried
26 S H -0.8032 Soluble
27 G H -0.9933 Soluble
28 F H -0.5268 Soluble
29 T H -0.6176 Soluble
30 F H 0.0000 Buried
35 N H -2.1378 Soluble
36 D H -0.9229 Soluble
37 Y H -0.2036 Soluble
38 A H 0.0000 Buried
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8611 Soluble
45 A H -1.2222 Soluble
46 P H -1.0731 Soluble
47 G H -1.4987 Soluble
48 K H -2.3834 Soluble
49 G H -1.5688 Soluble
50 L H 0.0000 Buried
51 E H -1.0891 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 T H 0.4092 Aggregation-prone
56 I H 0.0000 Buried
57 S H -0.4304 Soluble
58 W H -0.9722 Soluble
59 N H -1.7006 Soluble
62 S H -0.8749 Soluble
63 G H -0.7132 Soluble
64 S H -0.0611 Soluble
65 I H 0.9500 Aggregation-prone
66 G H 0.0000 Buried
67 Y H -0.2856 Soluble
68 A H 0.0000 Buried
69 D H -2.4340 Soluble
70 S H -1.6813 Soluble
71 V H 0.0000 Buried
72 K H -2.4319 Soluble
74 G H -1.5759 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.7423 Soluble
78 I H 0.0000 Buried
79 S H -0.3260 Soluble
80 R H -1.2814 Soluble
81 D H -1.9290 Soluble
82 N H -2.2107 Soluble
83 A H -1.6137 Soluble
84 K H -2.4820 Soluble
85 K H -2.1267 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6987 Soluble
89 L H 0.0000 Buried
90 Q H -1.5704 Soluble
91 M H 0.0000 Buried
92 N H -1.7562 Soluble
93 S H -1.7577 Soluble
94 L H 0.0000 Buried
95 R H -2.5393 Soluble
96 A H -1.7112 Soluble
97 E H -2.2448 Soluble
98 D H 0.0000 Buried
99 T H -0.8136 Soluble
100 A H 0.0000 Buried
101 L H -0.0611 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 K H 0.0000 Buried
107 D H 0.0000 Buried
108 I H 0.6324 Aggregation-prone
109 Q H 0.8268 Aggregation-prone
110 Y H 1.2298 Aggregation-prone
111 G H 0.1553 Aggregation-prone
111A N H 0.3678 Aggregation-prone
112A Y H 1.4322 Aggregation-prone
112 Y H 1.2270 Aggregation-prone
113 Y H 0.8512 Aggregation-prone
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.9338 Soluble
117 V H -0.3445 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.2038 Soluble
121 G H -0.4630 Soluble
122 T H -0.2271 Soluble
123 T H -0.3243 Soluble
124 V H 0.0000 Buried
125 T H -0.4308 Soluble
126 V H 0.0000 Buried
127 S H -0.8543 Soluble
128 S H -0.5221 Soluble
129 A H -0.4090 Soluble
130 S H -0.5533 Soluble
131 T H -0.7903 Soluble
132 K H -1.3762 Soluble
133 G H -1.5123 Soluble
134 P H -0.7182 Soluble
135 S H -0.5300 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2244 Soluble
139 L H 0.0000 Buried
140 A H -0.9155 Soluble
141 P H 0.0000 Buried
142 G H -0.6431 Soluble
143 G H -0.6527 Soluble
144 T H -0.4839 Soluble
145 A H -0.3275 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.5427 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.6769 Soluble
158 P H -0.9033 Soluble
159 V H -0.8095 Soluble
160 T H -0.6619 Soluble
161 V H -0.3953 Soluble
162 S H -0.4450 Soluble
163 W H 0.0000 Buried
164 N H -0.8389 Soluble
165 S H -0.7011 Soluble
166 G H -0.5513 Soluble
167 A H -0.2577 Soluble
168 L H -0.0166 Soluble
169 T H -0.1723 Soluble
170 S H -0.1772 Soluble
171 G H -0.2182 Soluble
172 V H 0.1683 Aggregation-prone
173 H H -0.3023 Soluble
174 T H 0.0107 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.3589 Soluble
177 A H 0.2303 Aggregation-prone
178 V H 0.4987 Aggregation-prone
179 L H 1.2963 Aggregation-prone
180 Q H 0.2397 Aggregation-prone
181 S H -0.0408 Soluble
182 S H -0.2168 Soluble
183 G H 0.0324 Aggregation-prone
184 L H 0.0979 Aggregation-prone
185 Y H 0.3772 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1253 Soluble
193 V H 0.0000 Buried
194 P H -0.6009 Soluble
195 S H -0.5863 Soluble
196 S H -0.6258 Soluble
197 S H -0.6230 Soluble
198 L H -0.8340 Soluble
199 G H -0.9982 Soluble
200 T H -0.7661 Soluble
201 Q H -1.3412 Soluble
202 T H -1.1705 Soluble
203 Y H 0.0000 Buried
204 I H -1.3504 Soluble
205 C H 0.0000 Buried
206 N H -1.5726 Soluble
207 V H 0.0000 Buried
208 N H -2.1290 Soluble
209 H H 0.0000 Buried
210 K H -2.7919 Soluble
211 P H -1.6421 Soluble
212 S H -1.9158 Soluble
213 N H -2.6251 Soluble
214 T H -2.1739 Soluble
215 K H -2.7205 Soluble
216 V H -1.6747 Soluble
217 D H -2.6266 Soluble
218 K H -2.1364 Soluble
219 K H -2.6219 Soluble
220 V H 0.0000 Buried
221 E H -2.5443 Soluble
222 P H -1.2614 Soluble
1 E L -1.6257 Soluble
2 I L 0.0000 Buried
3 V L 0.4747 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.7572 Soluble
6 Q L 0.0000 Buried
7 S L -0.6662 Soluble
8 P L -0.2060 Soluble
9 A L -0.2805 Soluble
10 T L -0.0851 Soluble
11 L L 0.0540 Aggregation-prone
12 S L -0.2766 Soluble
13 L L -0.8856 Soluble
14 S L -1.3523 Soluble
15 P L -1.6279 Soluble
16 G L -1.7684 Soluble
17 E L -2.4432 Soluble
18 R L -2.7081 Soluble
19 A L 0.0000 Buried
20 T L -0.5550 Soluble
21 L L 0.0000 Buried
22 S L -0.8445 Soluble
23 C L 0.0000 Buried
24 R L -2.3313 Soluble
25 A L -1.2152 Soluble
26 S L -1.0121 Soluble
27 Q L -1.5335 Soluble
28 S L -1.2865 Soluble
29 V L 0.0000 Buried
36 S L -0.5922 Soluble
37 S L -0.0948 Soluble
38 Y L 0.3257 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.5666 Soluble
46 P L -1.4190 Soluble
47 G L -1.4052 Soluble
48 Q L -1.8910 Soluble
49 A L -1.2402 Soluble
50 P L 0.0000 Buried
51 R L -1.1804 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.3603 Soluble
56 D L -0.2612 Soluble
57 A L 0.0000 Buried
65 S L -0.9922 Soluble
66 N L -1.2753 Soluble
67 R L -1.6869 Soluble
68 A L -1.1299 Soluble
69 T L -0.8009 Soluble
70 G L -0.7205 Soluble
71 I L 0.0000 Buried
72 P L -0.4908 Soluble
74 A L -0.4438 Soluble
75 R L -0.7141 Soluble
76 F L 0.0000 Buried
77 S L -0.7349 Soluble
78 G L -0.7163 Soluble
79 S L -0.7681 Soluble
80 G L -1.0895 Soluble
83 S L -0.8327 Soluble
84 G L -1.1376 Soluble
85 T L -1.7477 Soluble
86 D L -2.2233 Soluble
87 F L 0.0000 Buried
88 T L -0.7751 Soluble
89 L L 0.0000 Buried
90 T L -0.6282 Soluble
91 I L 0.0000 Buried
92 S L -1.4286 Soluble
93 S L -1.7268 Soluble
94 L L 0.0000 Buried
95 E L -1.8266 Soluble
96 P L -1.3408 Soluble
97 E L -1.9008 Soluble
98 D L 0.0000 Buried
99 F L -0.4668 Soluble
100 A L 0.0000 Buried
101 V L -0.0667 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 R L 0.0000 Buried
108 S L 0.0567 Aggregation-prone
109 N L -0.4495 Soluble
114 W L 0.2651 Aggregation-prone
115 P L -0.3431 Soluble
116 I L 0.0000 Buried
117 T L -0.1214 Soluble
118 F L -0.0090 Soluble
119 G L 0.0000 Buried
120 Q L -1.4192 Soluble
121 G L -0.8638 Soluble
122 T L 0.0000 Buried
123 R L -0.3548 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.5619 Soluble
127 K L -1.0376 Soluble
128 R L -0.8652 Soluble
129 T L -0.0699 Soluble
130 V L 0.3880 Aggregation-prone
131 A L 0.1031 Aggregation-prone
132 A L -0.1311 Soluble
133 P L 0.0000 Buried
134 S L -0.2203 Soluble
135 V L 0.0000 Buried
136 F L -0.0290 Soluble
137 I L -0.0088 Soluble
138 F L 0.0000 Buried
139 P L -0.8081 Soluble
140 P L -1.0072 Soluble
141 S L -1.7188 Soluble
142 D L -3.0623 Soluble
143 E L -3.2026 Soluble
144 Q L 0.0000 Buried
145 L L -2.2635 Soluble
146 K L -2.8043 Soluble
147 S L -1.7323 Soluble
148 G L -1.3314 Soluble
149 T L -0.8051 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.4085 Soluble
158 N L -0.7962 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L 0.0000 Buried
162 R L -2.0886 Soluble
163 E L -2.7393 Soluble
164 A L -2.0892 Soluble
165 K L -2.2782 Soluble
166 V L -1.1173 Soluble
167 Q L -0.5206 Soluble
168 W L 0.0000 Buried
169 K L -0.6571 Soluble
170 V L 0.0000 Buried
171 D L -1.6977 Soluble
172 N L -1.4964 Soluble
173 A L -0.3294 Soluble
174 L L 0.4010 Aggregation-prone
175 Q L -0.5580 Soluble
176 S L -0.7982 Soluble
177 G L -1.4598 Soluble
178 N L -1.8670 Soluble
179 S L -1.8899 Soluble
180 Q L -2.2099 Soluble
181 E L -2.1408 Soluble
182 S L -0.9072 Soluble
183 V L -0.6384 Soluble
184 T L -1.0625 Soluble
185 E L -2.2071 Soluble
186 Q L -1.6862 Soluble
187 D L -1.8386 Soluble
188 S L -1.8894 Soluble
189 K L -2.1908 Soluble
190 D L -1.6211 Soluble
191 S L -1.5548 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -1.0108 Soluble
199 L L 0.0000 Buried
200 T L -0.7527 Soluble
201 L L -0.7710 Soluble
202 S L -0.9701 Soluble
203 K L -1.9362 Soluble
204 A L -1.7362 Soluble
205 D L -2.4487 Soluble
206 Y L 0.0000 Buried
207 E L -3.4224 Soluble
208 K L -3.5615 Soluble
209 H L -3.2860 Soluble
210 K L -3.4334 Soluble
211 V L -2.1294 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.5605 Soluble
216 V L 0.0000 Buried
217 T L -1.1570 Soluble
218 H L 0.0000 Buried
219 Q L -1.6302 Soluble
220 G L -0.4084 Soluble
221 L L -0.2097 Soluble
222 S L -0.4467 Soluble
223 S L -0.4214 Soluble
224 P L -0.4929 Soluble
225 V L 0.0250 Aggregation-prone
226 T L -0.4651 Soluble
227 K L -0.7780 Soluble
228 S L -0.9207 Soluble
229 F L -1.2670 Soluble
230 N L -2.5022 Soluble
231 R L -3.2239 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6587 3.1869 CSV PDB
4.5 -0.6985 3.0812 CSV PDB
5.0 -0.7459 2.9466 CSV PDB
5.5 -0.7914 2.7991 CSV PDB
6.0 -0.8247 2.6547 CSV PDB
6.5 -0.8374 2.5293 CSV PDB
7.0 -0.8292 2.4347 CSV PDB
7.5 -0.8069 2.3682 CSV PDB
8.0 -0.7766 2.3208 CSV PDB
8.5 -0.7409 2.2897 CSV PDB
9.0 -0.7 2.2734 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH