Donzakimig1 / 5fuz

A4DAB089_5fuz_H_L | Source PDB: 5fuz | Chains: H, L | Done
99.13
Yes
10 Å
Yes
Avg A4D Score
-0.7065
Max Score
1.3898
Min Score
-3.6634
Total Score
-309.43
Score CDR
-0.4003
Score non-CDR
-0.7468

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAVSGIDLSNYAINWVRQAPGKGLEWIGIIWASGTTFYATWAKGRFTISRDNSKNTVYLQMNSLRAEDTAVYYCARTVPGYSTAPYFDLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSDIQMTQSPSSVSASVGDRVTITCQSSPSVWSNFLSWYQQKPGKAPKLLIYEASKLTSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCGGGYSSISDTTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6634
Maximum Score 1.3898
Average Score -0.7065
Total Score -309.4338

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9227 Soluble
2 V H -0.8893 Soluble
3 Q H -1.0662 Soluble
4 L H 0.0000 Buried
5 L H 0.2519 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5782 Soluble
8 G H -0.8636 Soluble
9 G H -0.3870 Soluble
11 G H -0.2584 Soluble
12 L H -0.0172 Soluble
13 V H 0.0000 Buried
14 Q H -1.5887 Soluble
15 P H -1.5510 Soluble
16 G H -1.3160 Soluble
17 G H -0.9770 Soluble
18 S H -1.2143 Soluble
19 L H -1.0056 Soluble
20 R H -2.1249 Soluble
21 L H 0.0000 Buried
22 S H -0.5500 Soluble
23 C H 0.0000 Buried
24 A H -0.4389 Soluble
25 V H 0.0000 Buried
26 S H -0.9581 Soluble
27 G H -1.1940 Soluble
28 I H -1.0873 Soluble
29 D H -2.3001 Soluble
30 L H 0.0000 Buried
35 S H -1.7580 Soluble
36 N H -1.5787 Soluble
37 Y H -0.6378 Soluble
38 A H -0.1826 Soluble
39 I H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.6368 Soluble
44 Q H -0.9488 Soluble
45 A H -1.2542 Soluble
46 P H -1.0049 Soluble
47 G H -1.4710 Soluble
48 K H -2.3144 Soluble
49 G H -1.5264 Soluble
50 L H 0.0000 Buried
51 E H -1.2434 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 I H 0.0000 Buried
56 I H 0.0000 Buried
57 W H -0.0988 Soluble
58 A H -0.5726 Soluble
59 S H -0.5522 Soluble
63 G H -0.6540 Soluble
64 T H -0.1754 Soluble
65 T H 0.0650 Aggregation-prone
66 F H 0.1917 Aggregation-prone
67 Y H 0.0000 Buried
68 A H 0.0000 Buried
69 T H -0.4763 Soluble
70 W H -0.2311 Soluble
71 A H 0.0000 Buried
72 K H -1.8737 Soluble
74 G H -1.5094 Soluble
75 R H -1.4158 Soluble
76 F H 0.0000 Buried
77 T H -0.9719 Soluble
78 I H 0.0000 Buried
79 S H -0.5550 Soluble
80 R H -1.1314 Soluble
81 D H -1.7400 Soluble
82 N H -2.2863 Soluble
83 S H -1.6775 Soluble
84 K H -2.6145 Soluble
85 N H -2.1282 Soluble
86 T H 0.0000 Buried
87 V H 0.0000 Buried
88 Y H -0.7009 Soluble
89 L H 0.0000 Buried
90 Q H -1.4125 Soluble
91 M H 0.0000 Buried
92 N H -1.3018 Soluble
93 S H -1.1477 Soluble
94 L H 0.0000 Buried
95 R H -2.0830 Soluble
96 A H -1.6044 Soluble
97 E H -2.1561 Soluble
98 D H 0.0000 Buried
99 T H -0.7153 Soluble
100 A H 0.0000 Buried
101 V H 0.1422 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.1138 Soluble
107 T H 0.0000 Buried
108 V H -0.0046 Soluble
109 P H -0.3976 Soluble
110 G H -0.2902 Soluble
111 Y H 0.1250 Aggregation-prone
111A S H 0.0915 Aggregation-prone
112A T H 0.1636 Aggregation-prone
112 A H 0.0000 Buried
113 P H 0.0000 Buried
114 Y H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.5697 Soluble
117 L H -0.2611 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.5090 Soluble
121 G H 0.0000 Buried
122 T H -0.1177 Soluble
123 L H 0.0432 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4241 Soluble
126 V H 0.0000 Buried
127 S H -0.9123 Soluble
128 S H -0.7017 Soluble
129 A H -0.5584 Soluble
130 S H -0.6899 Soluble
131 T H -0.9928 Soluble
132 K H -1.7739 Soluble
133 G H -1.8832 Soluble
134 P H 0.0000 Buried
135 S H -0.4808 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.8166 Soluble
139 L H 0.0000 Buried
140 A H -0.9781 Soluble
141 P H 0.0000 Buried
142 S H -1.2509 Soluble
143 S H -1.6032 Soluble
144 K H -2.1898 Soluble
145 S H -1.2758 Soluble
146 T H -1.0801 Soluble
147 S H -0.9939 Soluble
148 G H -1.1563 Soluble
149 G H -0.8641 Soluble
150 T H -0.6411 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.6486 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.6381 Soluble
164 P H -0.7221 Soluble
165 V H -0.6509 Soluble
166 T H -0.5475 Soluble
167 V H -0.3503 Soluble
168 S H -0.4046 Soluble
169 W H 0.0000 Buried
170 N H -0.6728 Soluble
171 S H -0.6757 Soluble
172 G H -0.5243 Soluble
173 A H -0.2435 Soluble
174 L H -0.0116 Soluble
175 T H -0.1768 Soluble
176 S H -0.1814 Soluble
177 G H -0.2325 Soluble
178 V H 0.1625 Aggregation-prone
179 H H -0.3322 Soluble
180 T H 0.0106 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3605 Soluble
183 A H 0.3798 Aggregation-prone
184 V H 0.8160 Aggregation-prone
185 L H 1.3898 Aggregation-prone
186 Q H 0.2018 Aggregation-prone
187 S H -0.0970 Soluble
188 S H -0.2801 Soluble
189 G H -0.0550 Soluble
190 L H 0.0115 Aggregation-prone
191 Y H 0.4607 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1805 Soluble
199 V H 0.0000 Buried
200 P H -0.5169 Soluble
201 S H -0.7499 Soluble
202 S H -0.5687 Soluble
203 S H -0.6484 Soluble
204 L H -0.7806 Soluble
205 G H -1.0276 Soluble
206 T H -0.8094 Soluble
207 Q H -1.1892 Soluble
208 T H -0.9626 Soluble
209 Y H 0.0000 Buried
210 I H -0.9708 Soluble
211 C H 0.0000 Buried
212 N H -1.3809 Soluble
213 V H 0.0000 Buried
214 N H -1.9604 Soluble
215 H H 0.0000 Buried
216 K H -2.7871 Soluble
217 P H -1.6705 Soluble
218 S H -1.9957 Soluble
219 N H -2.6466 Soluble
220 T H -2.2087 Soluble
221 K H -2.6793 Soluble
222 V H -1.6377 Soluble
223 D H -2.3535 Soluble
224 K H -1.6621 Soluble
225 K H -1.6813 Soluble
226 V H 0.0000 Buried
227 E H -2.1948 Soluble
228 P H -1.9485 Soluble
229 K H -2.3546 Soluble
1 D L -2.1262 Soluble
2 I L -1.4369 Soluble
3 Q L -1.8221 Soluble
4 M L -1.1478 Soluble
5 T L -1.1338 Soluble
6 Q L 0.0000 Buried
7 S L -0.6790 Soluble
8 P L -0.5004 Soluble
9 S L -0.7056 Soluble
10 S L -0.7275 Soluble
11 V L -0.4036 Soluble
12 S L -0.8685 Soluble
13 A L -1.2334 Soluble
14 S L -1.2720 Soluble
15 V L -0.6340 Soluble
16 G L -1.1067 Soluble
17 D L -2.2206 Soluble
18 R L -2.5356 Soluble
19 V L 0.0000 Buried
20 T L -0.6118 Soluble
21 I L 0.0000 Buried
22 T L -0.8252 Soluble
23 C L 0.0000 Buried
24 Q L -2.2141 Soluble
25 S L 0.0000 Buried
26 S L -1.3745 Soluble
27 P L -0.8036 Soluble
28 S L -0.8019 Soluble
29 V L 0.0000 Buried
30 W L 0.1068 Aggregation-prone
36 S L -0.6046 Soluble
37 N L -1.3621 Soluble
38 F L -0.4280 Soluble
39 L L 0.0000 Buried
40 S L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.8188 Soluble
46 P L -1.5253 Soluble
47 G L -1.8396 Soluble
48 K L -2.5285 Soluble
49 A L -1.5599 Soluble
50 P L 0.0000 Buried
51 K L -1.3762 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.5935 Soluble
56 E L -1.1311 Soluble
57 A L 0.0000 Buried
65 S L -1.1584 Soluble
66 K L -1.2897 Soluble
67 L L -0.4435 Soluble
68 T L -0.4574 Soluble
69 S L -0.4483 Soluble
70 G L -0.4785 Soluble
71 V L -0.2918 Soluble
72 P L -0.3585 Soluble
74 S L -0.4444 Soluble
75 R L -0.8349 Soluble
76 F L 0.0000 Buried
77 S L -0.5650 Soluble
78 G L -0.6183 Soluble
79 S L -1.0090 Soluble
80 G L -1.4670 Soluble
83 S L -1.4720 Soluble
84 G L -1.2102 Soluble
85 T L -1.6035 Soluble
86 D L -2.3965 Soluble
87 F L 0.0000 Buried
88 T L -0.8290 Soluble
89 L L 0.0000 Buried
90 T L -0.6479 Soluble
91 I L 0.0000 Buried
92 S L -1.4524 Soluble
93 S L -1.3295 Soluble
94 L L 0.0000 Buried
95 Q L -1.0755 Soluble
96 P L -1.5457 Soluble
97 E L -1.7162 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -0.7826 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 G L 0.0000 Buried
106 G L 0.0000 Buried
107 G L 0.0000 Buried
108 Y L 0.0000 Buried
109 S L -0.0890 Soluble
110 S L -0.1586 Soluble
113 I L 0.0399 Aggregation-prone
114 S L -0.3564 Soluble
115 D L -0.8184 Soluble
116 T L -0.3529 Soluble
117 T L -0.5129 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.0938 Soluble
121 G L -0.9372 Soluble
122 T L 0.0000 Buried
123 K L -1.4093 Soluble
124 V L 0.0000 Buried
125 E L -1.3654 Soluble
126 I L 0.0000 Buried
127 K L -1.7478 Soluble
128 R L -1.1112 Soluble
129 T L -0.1323 Soluble
130 V L 0.3707 Aggregation-prone
131 A L 0.1800 Aggregation-prone
132 A L -0.0771 Soluble
133 P L 0.0000 Buried
134 S L -0.1253 Soluble
135 V L 0.0999 Aggregation-prone
136 F L 0.1109 Aggregation-prone
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.5051 Soluble
140 P L -0.6775 Soluble
141 S L -1.3171 Soluble
142 D L -2.5087 Soluble
143 E L -2.1030 Soluble
144 Q L 0.0000 Buried
145 L L -2.0695 Soluble
146 K L -2.5755 Soluble
147 S L -1.6153 Soluble
148 G L -1.2181 Soluble
149 T L -0.9407 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9763 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.8673 Soluble
162 R L -3.1373 Soluble
163 E L -3.2708 Soluble
164 A L -2.4464 Soluble
165 K L -2.5031 Soluble
166 V L -1.2529 Soluble
167 Q L -0.7720 Soluble
168 W L 0.0000 Buried
169 K L -0.8227 Soluble
170 V L 0.0000 Buried
171 D L -2.0932 Soluble
172 N L -1.5057 Soluble
173 A L -0.5059 Soluble
174 L L 0.2742 Aggregation-prone
175 Q L -0.3901 Soluble
176 S L -0.4786 Soluble
177 G L -0.9321 Soluble
178 N L -0.8453 Soluble
179 S L -1.0923 Soluble
180 Q L -1.2759 Soluble
181 E L -1.1303 Soluble
182 S L -0.6164 Soluble
183 V L -0.6364 Soluble
184 T L -1.2040 Soluble
185 E L -2.3263 Soluble
186 Q L 0.0000 Buried
187 D L -2.1016 Soluble
188 S L -2.1469 Soluble
189 K L -2.5472 Soluble
190 D L -2.0292 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5705 Soluble
199 L L 0.0000 Buried
200 T L -0.4626 Soluble
201 L L -0.6009 Soluble
202 S L -1.1942 Soluble
203 K L -2.1444 Soluble
204 A L -1.8863 Soluble
205 D L -2.3595 Soluble
206 Y L 0.0000 Buried
207 E L -3.6628 Soluble
208 K L -3.6634 Soluble
209 H L -3.0132 Soluble
210 K L -3.1039 Soluble
211 V L -1.3447 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8725 Soluble
216 V L 0.0000 Buried
217 T L -1.1786 Soluble
218 H L 0.0000 Buried
219 Q L -1.7070 Soluble
220 G L -0.3854 Soluble
221 L L -0.1545 Soluble
222 S L -0.4044 Soluble
223 S L -0.3298 Soluble
224 P L -0.4225 Soluble
225 V L 0.2599 Aggregation-prone
226 T L -0.3279 Soluble
227 K L -0.5114 Soluble
228 S L -0.4585 Soluble
229 F L -0.7659 Soluble
230 N L -1.7127 Soluble
231 R L -2.3985 Soluble
232 G L -2.1707 Soluble
233 E L -2.4400 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7522 1.6094 CSV PDB
4.5 -0.7918 1.5949 CSV PDB
5.0 -0.837 1.5794 CSV PDB
5.5 -0.8786 1.5635 CSV PDB
6.0 -0.9056 1.5476 CSV PDB
6.5 -0.9099 1.5316 CSV PDB
7.0 -0.8923 1.5158 CSV PDB
7.5 -0.8603 1.5004 CSV PDB
8.0 -0.82 1.4864 CSV PDB
8.5 -0.7731 1.4751 CSV PDB
9.0 -0.7201 1.5772 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH