Atisnolerbart / 7mxl

A4DAB027_7mxl_J_K | Source PDB: 7mxl | Chains: J, K | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6891
Max Score
1.5045
Min Score
-3.5403
Total Score
-294.24
Score CDR
-0.3307
Score non-CDR
-0.7345

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGASVKVSCKASGYTFISYNIFWVRQATGQGLDWMGWMNPFRNNAGYAQKFQGRVTVTWDTSISTAYMELSSLSSEDTAIYYCAREHGSSWGFFDYWGQGTLVTVSSASTKGPSVFPLAPCSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSKTYTCNVDHKPSNTKVDKRVESKEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) J (Heavy), K (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5403
Maximum Score 1.5045
Average Score -0.6891
Total Score -294.2418

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q J -1.5406 Soluble
2 V J -0.9800 Soluble
3 Q J -1.4477 Soluble
4 L J 0.0000 Buried
5 V J -0.1471 Soluble
6 Q J -0.4576 Soluble
7 S J -0.7692 Soluble
8 G J -0.6742 Soluble
9 A J -0.3708 Soluble
11 E J -1.0820 Soluble
12 V J -0.4202 Soluble
13 K J -1.3058 Soluble
14 K J -2.0348 Soluble
15 P J -1.5343 Soluble
16 G J -1.1855 Soluble
17 A J -1.0033 Soluble
18 S J -1.3307 Soluble
19 V J 0.0000 Buried
20 K J -2.3180 Soluble
21 V J 0.0000 Buried
22 S J -0.6386 Soluble
23 C J 0.0000 Buried
24 K J -0.3383 Soluble
25 A J 0.0000 Buried
26 S J -0.6544 Soluble
27 G J -1.0756 Soluble
28 Y J -0.2917 Soluble
29 T J 0.1795 Aggregation-prone
30 F J 0.0000 Buried
35 I J 0.8523 Aggregation-prone
36 S J 0.6283 Aggregation-prone
37 Y J 0.4134 Aggregation-prone
38 N J -0.2092 Soluble
39 I J 0.0000 Buried
40 F J 0.0000 Buried
41 W J 0.0000 Buried
42 V J 0.0000 Buried
43 R J -0.3980 Soluble
44 Q J -0.5172 Soluble
45 A J -0.8290 Soluble
46 T J -0.4906 Soluble
47 G J -1.1475 Soluble
48 Q J -1.7987 Soluble
49 G J -1.3251 Soluble
50 L J 0.0000 Buried
51 D J -0.8734 Soluble
52 W J -0.2122 Soluble
53 M J 0.0000 Buried
54 G J 0.0000 Buried
55 W J 0.0882 Aggregation-prone
56 M J 0.0000 Buried
57 N J -0.7998 Soluble
58 P J 0.0000 Buried
59 F J 0.5922 Aggregation-prone
62 R J -1.8207 Soluble
63 N J -2.0441 Soluble
64 N J -2.1229 Soluble
65 A J -1.0228 Soluble
66 G J -0.3883 Soluble
67 Y J -0.6038 Soluble
68 A J 0.0000 Buried
69 Q J -2.4387 Soluble
70 K J -2.6965 Soluble
71 F J 0.0000 Buried
72 Q J -2.3683 Soluble
74 G J -1.6402 Soluble
75 R J -1.4699 Soluble
76 V J 0.0000 Buried
77 T J -0.8864 Soluble
78 V J 0.0000 Buried
79 T J -0.4925 Soluble
80 W J -0.1653 Soluble
81 D J -0.1356 Soluble
82 T J 0.2856 Aggregation-prone
83 S J 0.5694 Aggregation-prone
84 I J 1.5045 Aggregation-prone
85 S J 0.5620 Aggregation-prone
86 T J 0.0000 Buried
87 A J 0.0000 Buried
88 Y J -0.6120 Soluble
89 M J 0.0000 Buried
90 E J -1.9149 Soluble
91 L J 0.0000 Buried
92 S J -1.0986 Soluble
93 S J -0.8361 Soluble
94 L J 0.0000 Buried
95 S J -1.3315 Soluble
96 S J -1.5116 Soluble
97 E J -1.9383 Soluble
98 D J 0.0000 Buried
99 T J -0.5350 Soluble
100 A J 0.0000 Buried
101 I J 0.4923 Aggregation-prone
102 Y J 0.0000 Buried
103 Y J 0.0000 Buried
104 C J 0.0000 Buried
105 A J 0.0000 Buried
106 R J 0.0000 Buried
107 E J 0.0000 Buried
108 H J -0.5072 Soluble
109 G J -0.3139 Soluble
110 S J -0.3135 Soluble
111 S J -0.1820 Soluble
112 W J 0.2922 Aggregation-prone
113 G J 0.0000 Buried
114 F J 0.0000 Buried
115 F J 0.0000 Buried
116 D J -0.3787 Soluble
117 Y J -0.2645 Soluble
118 W J -0.4236 Soluble
119 G J 0.0000 Buried
120 Q J -1.3270 Soluble
121 G J -0.5734 Soluble
122 T J 0.0000 Buried
123 L J 0.3598 Aggregation-prone
124 V J 0.0000 Buried
125 T J -0.5280 Soluble
126 V J 0.0000 Buried
127 S J -1.0674 Soluble
128 S J -0.9392 Soluble
129 A J -0.6857 Soluble
130 S J -0.7364 Soluble
131 T J -0.7785 Soluble
132 K J -1.4170 Soluble
133 G J -1.7587 Soluble
134 P J -0.9816 Soluble
135 S J -0.6146 Soluble
136 V J 0.0000 Buried
137 F J 0.0000 Buried
138 P J -1.2856 Soluble
139 L J 0.0000 Buried
140 A J -0.7374 Soluble
141 P J -0.0793 Soluble
142 C J 0.1729 Aggregation-prone
143 S J -0.3901 Soluble
144 T J -0.3149 Soluble
145 A J -0.2108 Soluble
146 A J 0.0000 Buried
147 L J 0.0000 Buried
148 G J 0.0000 Buried
149 C J 0.0000 Buried
150 L J 0.0000 Buried
151 V J 0.0000 Buried
152 K J -0.4381 Soluble
153 D J -0.7429 Soluble
154 Y J 0.0000 Buried
155 F J 0.0000 Buried
156 P J -0.9466 Soluble
157 E J -0.8549 Soluble
158 P J -0.9528 Soluble
159 V J -0.8608 Soluble
160 T J -0.7526 Soluble
161 V J -0.4766 Soluble
162 S J -0.4240 Soluble
163 W J 0.0000 Buried
164 N J -0.8465 Soluble
165 S J -0.7030 Soluble
166 G J -0.3951 Soluble
167 A J -0.1838 Soluble
168 L J -0.0557 Soluble
169 T J -0.2074 Soluble
170 S J -0.3106 Soluble
171 G J -0.3893 Soluble
172 V J -0.0092 Soluble
173 H J -0.5120 Soluble
174 T J -0.2100 Soluble
175 F J 0.0000 Buried
176 P J -0.2681 Soluble
177 A J 0.4245 Aggregation-prone
178 V J 0.8195 Aggregation-prone
179 L J 1.3401 Aggregation-prone
180 Q J 0.3339 Aggregation-prone
181 S J -0.0181 Soluble
182 S J -0.2680 Soluble
183 G J -0.1124 Soluble
184 L J 0.0000 Buried
185 Y J 0.2542 Aggregation-prone
186 S J 0.3007 Aggregation-prone
187 L J 0.3197 Aggregation-prone
188 S J 0.0000 Buried
189 S J 0.0000 Buried
190 V J 0.0000 Buried
191 V J 0.0000 Buried
192 T J -0.1066 Soluble
193 V J -0.1232 Soluble
194 P J -0.4389 Soluble
195 S J -0.7865 Soluble
196 S J -0.8489 Soluble
197 S J -1.0374 Soluble
198 K J -2.1542 Soluble
199 T J -1.8239 Soluble
200 Y J -1.2145 Soluble
201 T J -1.7142 Soluble
202 C J 0.0000 Buried
203 N J -1.8742 Soluble
204 V J 0.0000 Buried
205 D J -2.4559 Soluble
206 H J 0.0000 Buried
207 K J -2.9377 Soluble
208 P J -1.7875 Soluble
209 S J -2.0483 Soluble
210 N J -2.6574 Soluble
211 T J -2.2926 Soluble
212 K J -2.8955 Soluble
213 V J -1.8153 Soluble
214 D J -2.8952 Soluble
215 K J -2.4117 Soluble
216 R J -3.0403 Soluble
217 V J 0.0000 Buried
218 E J -2.8886 Soluble
219 S J -2.3225 Soluble
220 K J -2.3181 Soluble
1 E K -1.6171 Soluble
2 I K 0.0000 Buried
3 V K 0.7439 Aggregation-prone
4 L K 0.0000 Buried
5 T K -0.4684 Soluble
6 Q K 0.0000 Buried
7 S K -0.6819 Soluble
8 P K -0.5696 Soluble
9 G K -0.9562 Soluble
10 T K -0.6349 Soluble
11 L K 0.0000 Buried
12 S K -0.5275 Soluble
13 L K -0.7715 Soluble
14 S K -1.2379 Soluble
15 P K -1.6734 Soluble
16 G K -2.1925 Soluble
17 E K -2.6630 Soluble
18 R K -2.9353 Soluble
19 A K 0.0000 Buried
20 T K -0.6494 Soluble
21 L K 0.0000 Buried
22 S K -0.6293 Soluble
23 C K 0.0000 Buried
24 R K -1.2673 Soluble
25 A K 0.0000 Buried
26 S K -0.8213 Soluble
27 Q K -1.4936 Soluble
28 S K -1.2252 Soluble
29 V K 0.0000 Buried
30 S K -0.5099 Soluble
36 S K -0.3606 Soluble
37 S K -0.4433 Soluble
38 Y K 0.0956 Aggregation-prone
39 L K 0.0000 Buried
40 A K 0.0000 Buried
41 W K 0.0000 Buried
42 Y K 0.0000 Buried
43 Q K 0.0000 Buried
44 Q K 0.0000 Buried
45 K K -1.4167 Soluble
46 P K -1.3056 Soluble
47 G K -1.3749 Soluble
48 Q K -2.0290 Soluble
49 A K -1.2683 Soluble
50 P K 0.0000 Buried
51 R K -1.2277 Soluble
52 L K 0.0000 Buried
53 L K 0.0000 Buried
54 I K 0.0000 Buried
55 Y K -0.0807 Soluble
56 G K -0.1740 Soluble
57 A K 0.0000 Buried
65 S K -0.7931 Soluble
66 S K -1.0437 Soluble
67 R K -2.1443 Soluble
68 A K -1.0440 Soluble
69 T K -0.6769 Soluble
70 G K -0.8794 Soluble
71 I K -1.0873 Soluble
72 P K -1.3285 Soluble
74 D K -2.4818 Soluble
75 R K -2.1574 Soluble
76 F K 0.0000 Buried
77 S K -1.1418 Soluble
78 G K -0.7600 Soluble
79 S K -0.7050 Soluble
80 G K -0.9574 Soluble
83 S K -0.9874 Soluble
84 G K -1.0778 Soluble
85 T K -1.3817 Soluble
86 D K -1.6053 Soluble
87 F K 0.0000 Buried
88 T K -0.6303 Soluble
89 L K 0.0000 Buried
90 T K -0.8378 Soluble
91 I K 0.0000 Buried
92 S K -2.5134 Soluble
93 R K -3.3496 Soluble
94 L K 0.0000 Buried
95 E K -2.4721 Soluble
96 P K -1.8351 Soluble
97 E K -2.2735 Soluble
98 D K 0.0000 Buried
99 F K -0.7292 Soluble
100 A K 0.0000 Buried
101 V K -0.3057 Soluble
102 Y K 0.0000 Buried
103 Y K 0.0000 Buried
104 C K 0.0000 Buried
105 Q K 0.0000 Buried
106 Q K 0.0000 Buried
107 Y K 0.0000 Buried
108 G K -0.1415 Soluble
109 S K -0.7435 Soluble
114 S K -0.6700 Soluble
115 P K -0.6251 Soluble
116 W K -0.3326 Soluble
117 T K -0.0666 Soluble
118 F K 0.0000 Buried
119 G K -0.4964 Soluble
120 Q K -1.4675 Soluble
121 G K -1.0631 Soluble
122 T K 0.0000 Buried
123 K K -0.9649 Soluble
124 V K 0.0000 Buried
125 E K -0.5483 Soluble
126 I K -0.6518 Soluble
127 K K -1.0233 Soluble
128 R K -0.7218 Soluble
129 T K 0.2578 Aggregation-prone
130 V K 1.2031 Aggregation-prone
131 A K 0.4400 Aggregation-prone
132 A K 0.0424 Aggregation-prone
133 P K 0.0000 Buried
134 S K -0.0723 Soluble
135 V K 0.0000 Buried
136 F K 0.2553 Aggregation-prone
137 I K 0.2016 Aggregation-prone
138 F K 0.0000 Buried
139 P K 0.0000 Buried
140 P K -0.6074 Soluble
141 S K 0.0000 Buried
142 D K -2.7623 Soluble
143 E K -2.6239 Soluble
144 Q K 0.0000 Buried
145 L K -2.0575 Soluble
146 K K -2.7579 Soluble
147 S K -1.7880 Soluble
148 G K -1.5095 Soluble
149 T K -1.1687 Soluble
150 A K 0.0000 Buried
151 S K 0.0000 Buried
152 V K 0.0000 Buried
153 V K 0.0000 Buried
154 C K 0.0000 Buried
155 L K 0.0000 Buried
156 L K 0.0000 Buried
157 N K 0.0000 Buried
158 N K -0.8065 Soluble
159 F K 0.0000 Buried
160 Y K 0.0000 Buried
161 P K -1.5970 Soluble
162 R K -2.9719 Soluble
163 E K -3.2811 Soluble
164 A K -2.4365 Soluble
165 K K -2.6860 Soluble
166 V K -1.3302 Soluble
167 Q K -0.6075 Soluble
168 W K 0.0000 Buried
169 K K -0.8966 Soluble
170 V K 0.0000 Buried
171 D K -1.8701 Soluble
172 N K -1.6608 Soluble
173 A K -0.4536 Soluble
174 L K 0.4683 Aggregation-prone
175 Q K -0.3795 Soluble
176 S K -0.4884 Soluble
177 G K -0.7213 Soluble
178 N K -0.5805 Soluble
179 S K -0.8184 Soluble
180 Q K -0.9938 Soluble
181 E K -1.7862 Soluble
182 S K -0.8547 Soluble
183 V K -0.9145 Soluble
184 T K -1.0304 Soluble
185 E K -2.1640 Soluble
186 Q K -2.0115 Soluble
187 D K -2.1022 Soluble
188 S K -1.8355 Soluble
189 K K -2.2830 Soluble
190 D K -1.7430 Soluble
191 S K -1.7283 Soluble
192 T K 0.0000 Buried
193 Y K 0.0000 Buried
194 S K 0.0000 Buried
195 L K 0.0000 Buried
196 S K 0.0000 Buried
197 S K 0.0000 Buried
198 T K -0.5004 Soluble
199 L K 0.0000 Buried
200 T K -0.2275 Soluble
201 L K -0.5173 Soluble
202 S K -1.1116 Soluble
203 K K -1.8795 Soluble
204 A K -1.8398 Soluble
205 D K -2.2774 Soluble
206 Y K 0.0000 Buried
207 E K -3.5403 Soluble
208 K K -3.4508 Soluble
209 H K -2.8269 Soluble
210 K K -2.9202 Soluble
211 V K -1.3417 Soluble
212 Y K 0.0000 Buried
213 A K 0.0000 Buried
214 C K 0.0000 Buried
215 E K 0.0000 Buried
216 V K 0.0000 Buried
217 T K -1.1051 Soluble
218 H K 0.0000 Buried
219 Q K -1.7080 Soluble
220 G K -0.2296 Soluble
221 L K -0.0977 Soluble
222 S K -0.4265 Soluble
223 S K -0.2698 Soluble
224 P K -0.0823 Soluble
225 V K 0.6418 Aggregation-prone
226 T K -0.0133 Soluble
227 K K -0.4060 Soluble
228 S K -0.4775 Soluble
229 F K 0.0000 Buried
230 N K -1.7015 Soluble
231 R K -2.3227 Soluble
232 G K -1.9815 Soluble
233 E K -2.1310 Soluble
234 C K -0.7768 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.72 1.9766 CSV PDB
4.5 -0.7604 1.9755 CSV PDB
5.0 -0.8092 1.9755 CSV PDB
5.5 -0.8565 1.9755 CSV PDB
6.0 -0.8907 1.9755 CSV PDB
6.5 -0.9023 1.9755 CSV PDB
7.0 -0.8907 1.9755 CSV PDB
7.5 -0.863 1.9755 CSV PDB
8.0 -0.8266 1.9755 CSV PDB
8.5 -0.7844 1.9754 CSV PDB
9.0 -0.7369 1.9754 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH