Amubarvimab / 7x2k

A4DAB014_7x2k_H_L | Source PDB: 7x2k | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6771
Max Score
1.9265
Min Score
-3.701
Total Score
-286.42
Score CDR
-0.0279
Score non-CDR
-0.7525

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGITVSSNYMNWVRQAPGKGLEWVSLIYSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYHCARDLVVYGMDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.701
Maximum Score 1.9265
Average Score -0.6771
Total Score -286.4232

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.9655 Aggregation-prone
3 Q H -0.2934 Soluble
4 L H 0.0000 Buried
5 V H 0.7493 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.3788 Soluble
8 G H -0.9505 Soluble
9 G H -0.5984 Soluble
11 G H -0.1639 Soluble
12 L H 0.3175 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.5846 Soluble
15 P H -1.7576 Soluble
16 G H -1.4634 Soluble
17 G H -1.1403 Soluble
18 S H -1.4871 Soluble
19 L H -1.2029 Soluble
20 R H -2.2721 Soluble
21 L H 0.0000 Buried
22 S H -0.6023 Soluble
23 C H 0.0000 Buried
24 A H -0.2823 Soluble
25 A H -0.2510 Soluble
26 S H -0.1112 Soluble
27 G H -0.1562 Soluble
28 I H 0.0000 Buried
29 T H -0.6212 Soluble
30 V H 0.0000 Buried
35 S H -0.8572 Soluble
36 S H -0.4680 Soluble
37 N H -0.0622 Soluble
38 Y H 0.3973 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8565 Soluble
45 A H -1.2333 Soluble
46 P H -1.0406 Soluble
47 G H -1.4935 Soluble
48 K H -2.4086 Soluble
49 G H -1.6796 Soluble
50 L H 0.0000 Buried
51 E H -1.1152 Soluble
52 W H -0.2427 Soluble
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 L H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H 0.1788 Aggregation-prone
58 S H -0.3409 Soluble
59 G H -0.5813 Soluble
63 G H -0.4981 Soluble
64 S H -0.0966 Soluble
65 T H 0.2523 Aggregation-prone
66 Y H 0.2548 Aggregation-prone
67 Y H -0.5687 Soluble
68 A H -1.2552 Soluble
69 D H -2.3713 Soluble
70 S H -1.7930 Soluble
71 V H 0.0000 Buried
72 K H -2.5186 Soluble
74 G H -1.8535 Soluble
75 R H -1.5536 Soluble
76 F H 0.0000 Buried
77 T H -0.8830 Soluble
78 I H 0.0000 Buried
79 S H -0.7151 Soluble
80 R H -1.4859 Soluble
81 D H -2.1013 Soluble
82 N H -2.4618 Soluble
83 S H -1.9811 Soluble
84 K H -2.5893 Soluble
85 N H -2.0074 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.4719 Soluble
91 M H 0.0000 Buried
92 N H -1.9660 Soluble
93 S H -1.4353 Soluble
94 L H 0.0000 Buried
95 R H -2.5414 Soluble
96 A H -1.8298 Soluble
97 E H -2.3112 Soluble
98 D H 0.0000 Buried
99 T H -0.7988 Soluble
100 A H 0.0000 Buried
101 V H -0.0422 Soluble
102 Y H 0.0000 Buried
103 H H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.1841 Soluble
107 D H 0.0000 Buried
108 L H 1.3685 Aggregation-prone
109 V H 1.4947 Aggregation-prone
110 V H 1.9265 Aggregation-prone
113 Y H 1.7443 Aggregation-prone
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.3207 Soluble
117 V H -0.2023 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.3653 Soluble
121 G H 0.0000 Buried
122 T H -0.2339 Soluble
123 T H -0.1981 Soluble
124 V H 0.0000 Buried
125 T H -0.4788 Soluble
126 V H 0.0000 Buried
127 S H -0.9444 Soluble
128 S H -0.6999 Soluble
129 A H -0.4933 Soluble
130 S H -0.5726 Soluble
131 T H -0.6681 Soluble
132 K H -1.2405 Soluble
133 G H -1.4448 Soluble
134 P H 0.0000 Buried
135 S H -0.5005 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1464 Soluble
139 L H 0.0000 Buried
140 A H -0.8224 Soluble
141 P H 0.0000 Buried
142 S H -0.1321 Soluble
144 T H -0.2824 Soluble
145 A H -0.1690 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H -0.3122 Soluble
153 D H -0.4424 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.7886 Soluble
157 E H -0.9341 Soluble
158 P H -1.0392 Soluble
159 V H -0.8794 Soluble
160 T H -0.7280 Soluble
161 V H -0.3894 Soluble
162 S H -0.3868 Soluble
163 W H 0.0000 Buried
164 N H -0.8168 Soluble
165 S H -0.6329 Soluble
166 G H -0.4961 Soluble
167 A H -0.2277 Soluble
168 L H 0.0344 Aggregation-prone
169 T H -0.1440 Soluble
170 S H -0.1582 Soluble
171 G H -0.1570 Soluble
172 V H 0.2147 Aggregation-prone
173 H H -0.2754 Soluble
174 T H 0.0317 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.3064 Soluble
177 A H 0.1735 Aggregation-prone
178 V H 0.5787 Aggregation-prone
179 L H 1.2884 Aggregation-prone
180 Q H 0.3537 Aggregation-prone
181 S H 0.0016 Aggregation-prone
182 S H -0.1559 Soluble
183 G H 0.1124 Aggregation-prone
184 L H 0.0721 Aggregation-prone
185 Y H 0.3350 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.0800 Soluble
193 V H 0.0000 Buried
194 P H -0.4097 Soluble
195 S H -0.4768 Soluble
196 S H -0.4457 Soluble
197 S H -0.5835 Soluble
198 L H -0.7417 Soluble
199 G H -1.0483 Soluble
200 T H -0.8070 Soluble
201 Q H -1.2018 Soluble
202 T H -1.1629 Soluble
203 Y H 0.0000 Buried
204 I H -1.1485 Soluble
205 C H 0.0000 Buried
206 N H -1.4258 Soluble
207 V H 0.0000 Buried
208 N H -2.3198 Soluble
209 H H 0.0000 Buried
210 K H -2.8822 Soluble
211 P H -1.7522 Soluble
212 S H -1.8783 Soluble
213 N H -2.6315 Soluble
214 T H -2.1927 Soluble
215 K H -2.8224 Soluble
216 V H -1.7491 Soluble
217 D H -2.3012 Soluble
218 K H -1.9583 Soluble
219 K H -2.1757 Soluble
220 V H 0.0000 Buried
221 E H -2.2967 Soluble
222 P H -1.2148 Soluble
1 E L -1.5100 Soluble
2 I L -0.2069 Soluble
3 V L 0.7501 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.7611 Soluble
6 Q L 0.0000 Buried
7 S L -0.8704 Soluble
8 P L -0.5038 Soluble
9 G L -0.8545 Soluble
10 T L -0.4332 Soluble
11 L L -0.1861 Soluble
12 S L -0.4367 Soluble
13 L L -0.8435 Soluble
14 S L -1.4824 Soluble
15 P L -1.9076 Soluble
16 G L -2.1801 Soluble
17 E L -2.8643 Soluble
18 R L -3.1394 Soluble
19 A L 0.0000 Buried
20 T L -0.5336 Soluble
21 L L 0.0000 Buried
22 S L -0.8980 Soluble
23 C L 0.0000 Buried
24 R L -2.2379 Soluble
25 A L 0.0000 Buried
26 S L -0.8710 Soluble
27 Q L -1.5659 Soluble
28 S L -1.1761 Soluble
29 V L -0.4333 Soluble
30 S L -0.2975 Soluble
36 S L -0.2337 Soluble
37 S L 0.4482 Aggregation-prone
38 Y L 0.9353 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -0.9453 Soluble
45 K L -1.4854 Soluble
46 P L -1.3622 Soluble
47 G L -1.4923 Soluble
48 Q L -2.1009 Soluble
49 A L -1.3885 Soluble
50 P L 0.0000 Buried
51 R L -1.1624 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.4842 Aggregation-prone
56 G L 0.2905 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.6985 Soluble
66 S L -0.5542 Soluble
67 R L -1.3241 Soluble
68 A L 0.0000 Buried
69 T L -0.6294 Soluble
70 G L -0.9065 Soluble
71 I L 0.0000 Buried
72 P L -1.3738 Soluble
74 D L -2.4023 Soluble
75 R L -2.1421 Soluble
76 F L 0.0000 Buried
77 S L -0.9137 Soluble
78 G L -0.5558 Soluble
79 S L -0.7164 Soluble
80 G L -1.1073 Soluble
83 S L -1.0268 Soluble
84 G L -1.1036 Soluble
85 T L -1.6735 Soluble
86 D L -2.0691 Soluble
87 F L 0.0000 Buried
88 T L -0.7821 Soluble
89 L L 0.0000 Buried
90 T L -0.8098 Soluble
91 I L 0.0000 Buried
92 S L -2.3982 Soluble
93 R L -3.5778 Soluble
94 L L 0.0000 Buried
95 E L -2.5291 Soluble
96 P L -1.8450 Soluble
97 E L -2.3309 Soluble
98 D L 0.0000 Buried
99 F L -0.6313 Soluble
100 A L 0.0000 Buried
101 V L -0.2454 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 G L -0.6698 Soluble
114 S L -0.5586 Soluble
115 S L 0.0000 Buried
116 P L 0.0000 Buried
117 T L -0.2412 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.4953 Soluble
121 G L -1.1210 Soluble
122 T L 0.0000 Buried
123 K L -0.6079 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.6552 Soluble
127 K L -1.3692 Soluble
128 R L -1.0172 Soluble
129 T L -0.1414 Soluble
130 V L 0.3230 Aggregation-prone
131 A L -0.0079 Soluble
132 A L -0.0761 Soluble
133 P L 0.0000 Buried
134 S L -0.0526 Soluble
135 V L 0.0006 Aggregation-prone
136 F L 0.0389 Aggregation-prone
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.5342 Soluble
140 P L 0.0000 Buried
141 S L -1.7291 Soluble
142 D L -3.1034 Soluble
143 E L -3.2850 Soluble
144 Q L 0.0000 Buried
145 L L -2.2773 Soluble
146 K L -2.9642 Soluble
147 S L -1.8345 Soluble
148 G L -1.4183 Soluble
149 T L -1.2933 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7932 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6953 Soluble
162 R L -2.8648 Soluble
163 E L -3.0977 Soluble
164 A L -2.2006 Soluble
165 K L -2.1570 Soluble
166 V L -0.8603 Soluble
167 Q L -0.4472 Soluble
168 W L 0.0000 Buried
169 K L -0.4704 Soluble
170 V L 0.0000 Buried
171 D L -1.9576 Soluble
172 N L -1.5275 Soluble
173 A L -0.2724 Soluble
174 L L 0.7220 Aggregation-prone
175 Q L -0.2246 Soluble
176 S L -0.3918 Soluble
177 G L -0.9416 Soluble
178 N L -0.8794 Soluble
179 S L -1.0323 Soluble
180 Q L -1.1469 Soluble
181 E L -1.3753 Soluble
182 S L -0.7712 Soluble
183 V L -0.7170 Soluble
184 T L -1.1906 Soluble
185 E L -2.2583 Soluble
186 Q L -1.8391 Soluble
187 D L -1.9073 Soluble
188 S L -1.7024 Soluble
189 K L -2.1766 Soluble
190 D L -1.5555 Soluble
191 S L -1.6245 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5360 Soluble
199 L L 0.0000 Buried
200 T L -0.4860 Soluble
201 L L -0.7657 Soluble
202 S L -1.2055 Soluble
203 K L -2.2877 Soluble
204 A L -2.1277 Soluble
205 D L -2.9251 Soluble
206 Y L 0.0000 Buried
207 E L -3.7010 Soluble
208 K L -3.6700 Soluble
209 H L -2.8943 Soluble
210 K L -2.6324 Soluble
211 V L -1.1214 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6624 Soluble
216 V L 0.0000 Buried
217 T L -1.1071 Soluble
218 H L 0.0000 Buried
219 Q L -1.6743 Soluble
220 G L -0.3720 Soluble
221 L L -0.2079 Soluble
222 S L -0.4333 Soluble
223 S L -0.4041 Soluble
224 P L -0.5113 Soluble
225 V L -0.0175 Soluble
226 T L -0.4671 Soluble
227 K L -0.7148 Soluble
228 S L -0.5656 Soluble
229 F L -0.7361 Soluble
230 N L -1.5956 Soluble
231 R L -2.0490 Soluble
232 G L -1.8026 Soluble
233 E L -2.0428 Soluble
234 C L -0.4000 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6824 1.7517 CSV PDB
4.5 -0.7262 1.7517 CSV PDB
5.0 -0.778 1.7517 CSV PDB
5.5 -0.8289 1.7517 CSV PDB
6.0 -0.8687 1.7517 CSV PDB
6.5 -0.8884 1.7517 CSV PDB
7.0 -0.8855 1.7516 CSV PDB
7.5 -0.8662 1.7515 CSV PDB
8.0 -0.8373 1.7512 CSV PDB
8.5 -0.8012 1.7502 CSV PDB
9.0 -0.7585 1.7472 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH