Vonlerolizumab / 7yk4

A4DAB300_7yk4_I_M | Source PDB: 7yk4 | Chains: I, M | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7491
Max Score
1.6336
Min Score
-3.6878
Total Score
-319.86
Score CDR
-0.6449
Score non-CDR
-0.7587

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGASVKVSCKASYTFTDSYMSWVRQAPGQGLEWIGDMYPDNGDSSYNQKFRERVTITRDTSTSTAYLELSSLRSEDTAVYYCVLAPRWYFSVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVGDRVTITCRASQDISNYLNWYQQKPGKAPKLLIYYTSRLRSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGHTLPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) I (Heavy), M (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6878
Maximum Score 1.6336
Average Score -0.7491
Total Score -319.856

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V I 1.6336 Aggregation-prone
3 Q I 0.0361 Aggregation-prone
4 L I 0.0000 Buried
5 V I 0.5583 Aggregation-prone
6 Q I 0.0856 Aggregation-prone
7 S I -0.3853 Soluble
8 G I -0.6662 Soluble
9 A I -0.8057 Soluble
11 E I -1.2864 Soluble
12 V I -0.8857 Soluble
13 K I -1.6476 Soluble
14 K I -2.4850 Soluble
15 P I -2.0639 Soluble
16 G I -1.5902 Soluble
17 A I -1.2528 Soluble
18 S I -1.2570 Soluble
19 V I 0.0000 Buried
20 K I -2.1229 Soluble
21 V I 0.0000 Buried
22 S I -0.5959 Soluble
23 C I 0.0000 Buried
24 K I -0.4580 Soluble
25 A I 0.1168 Aggregation-prone
26 S I 0.2935 Aggregation-prone
27 Y I 1.4227 Aggregation-prone
28 T I 0.3669 Aggregation-prone
29 F I 0.0000 Buried
30 T I -1.5039 Soluble
36 D I -1.9525 Soluble
37 S I -0.9501 Soluble
38 Y I -0.4639 Soluble
39 M I 0.0000 Buried
40 S I 0.0000 Buried
41 W I 0.0000 Buried
42 V I 0.0000 Buried
43 R I 0.0000 Buried
44 Q I -0.6394 Soluble
45 A I -1.0315 Soluble
46 P I -0.8129 Soluble
47 G I -1.2247 Soluble
48 Q I -1.8717 Soluble
49 G I -1.3775 Soluble
50 L I 0.0000 Buried
51 E I -0.7333 Soluble
52 W I 0.0000 Buried
53 I I 0.0000 Buried
54 G I 0.0000 Buried
55 D I 0.0000 Buried
56 M I 0.0000 Buried
57 Y I -1.5865 Soluble
58 P I 0.0000 Buried
59 D I -3.1275 Soluble
62 N I -2.9060 Soluble
63 G I -2.5470 Soluble
64 D I -2.5931 Soluble
65 S I -1.1460 Soluble
66 S I -0.4767 Soluble
67 Y I -0.8049 Soluble
68 N I -1.6919 Soluble
69 Q I -2.9845 Soluble
70 K I -3.3104 Soluble
71 F I 0.0000 Buried
72 R I -3.6878 Soluble
74 E I -3.2423 Soluble
75 R I -2.2288 Soluble
76 V I 0.0000 Buried
77 T I -1.1351 Soluble
78 I I 0.0000 Buried
79 T I -1.1195 Soluble
80 R I -1.6664 Soluble
81 D I -1.3697 Soluble
82 T I -1.0841 Soluble
83 S I -0.6190 Soluble
84 T I -0.6281 Soluble
85 S I -0.7263 Soluble
86 T I 0.0000 Buried
87 A I 0.0000 Buried
88 Y I -0.7899 Soluble
89 L I 0.0000 Buried
90 E I -1.5898 Soluble
91 L I 0.0000 Buried
92 S I -1.3880 Soluble
93 S I -1.4034 Soluble
94 L I 0.0000 Buried
95 R I -3.0734 Soluble
96 S I -2.2403 Soluble
97 E I -2.5336 Soluble
98 D I 0.0000 Buried
99 T I -0.9646 Soluble
100 A I 0.0000 Buried
101 V I 0.1882 Aggregation-prone
102 Y I 0.0000 Buried
103 Y I 0.0000 Buried
104 C I 0.0000 Buried
105 V I 0.0000 Buried
106 L I 0.0000 Buried
107 A I 0.0000 Buried
108 P I -0.4775 Soluble
109 R I -0.6097 Soluble
113 W I 0.0000 Buried
114 Y I 0.2897 Aggregation-prone
115 F I 0.0000 Buried
116 S I -0.1094 Soluble
117 V I 0.1920 Aggregation-prone
118 W I -0.4140 Soluble
119 G I 0.0000 Buried
120 Q I -1.3294 Soluble
121 G I 0.0000 Buried
122 T I 0.0000 Buried
123 L I -0.1893 Soluble
124 V I 0.0000 Buried
125 T I 0.0000 Buried
126 V I 0.0000 Buried
127 S I -1.2522 Soluble
128 S I -0.9271 Soluble
129 A I -0.7047 Soluble
130 S I -0.9051 Soluble
131 T I -1.3107 Soluble
132 K I -2.0725 Soluble
133 G I -1.8712 Soluble
134 P I -0.9526 Soluble
135 S I -0.4864 Soluble
136 V I 0.0000 Buried
137 F I 0.0000 Buried
138 P I -0.9938 Soluble
139 L I 0.0000 Buried
140 A I -1.1994 Soluble
141 P I 0.0000 Buried
142 S I -1.2273 Soluble
143 S I -1.0292 Soluble
144 K I -1.1887 Soluble
145 S I 0.0000 Buried
146 T I -0.7465 Soluble
147 S I -0.7349 Soluble
148 G I -0.8381 Soluble
149 G I -0.8634 Soluble
150 T I -0.6091 Soluble
151 A I 0.0000 Buried
152 A I 0.0000 Buried
153 L I 0.0000 Buried
154 G I 0.0000 Buried
155 C I 0.0000 Buried
156 L I 0.0000 Buried
157 V I 0.0000 Buried
158 K I 0.0000 Buried
159 D I -0.9217 Soluble
160 Y I 0.0000 Buried
161 F I 0.0000 Buried
162 P I 0.0000 Buried
163 E I -0.6036 Soluble
164 P I -0.8222 Soluble
165 V I -0.7367 Soluble
166 T I -0.6396 Soluble
167 V I -0.4585 Soluble
168 S I -0.4457 Soluble
169 W I 0.0000 Buried
170 N I -0.7167 Soluble
171 S I -0.6445 Soluble
172 G I -0.5739 Soluble
173 A I -0.2655 Soluble
174 L I -0.0479 Soluble
175 T I -0.2451 Soluble
176 S I -0.2978 Soluble
177 G I -0.3729 Soluble
178 V I 0.0992 Aggregation-prone
179 H I -0.3192 Soluble
180 T I 0.0249 Aggregation-prone
181 F I 0.0000 Buried
182 P I -0.5234 Soluble
183 A I 0.2373 Aggregation-prone
184 V I 0.6772 Aggregation-prone
185 L I 1.4148 Aggregation-prone
186 Q I 0.3995 Aggregation-prone
187 S I 0.0012 Aggregation-prone
188 S I -0.2309 Soluble
189 G I 0.0947 Aggregation-prone
190 L I -0.0283 Soluble
191 Y I 0.4398 Aggregation-prone
192 S I 0.0000 Buried
193 L I 0.0000 Buried
194 S I 0.0000 Buried
195 S I 0.0000 Buried
196 V I 0.0000 Buried
197 V I 0.0000 Buried
198 T I -0.1461 Soluble
199 V I 0.0000 Buried
200 P I -0.5196 Soluble
201 S I -0.5630 Soluble
202 S I -0.5840 Soluble
203 S I -0.6429 Soluble
204 L I -0.8447 Soluble
205 G I -1.0639 Soluble
206 T I -0.8426 Soluble
207 Q I -1.5040 Soluble
208 T I -1.2347 Soluble
209 Y I 0.0000 Buried
210 I I -1.0298 Soluble
211 C I 0.0000 Buried
212 N I -1.5428 Soluble
213 V I 0.0000 Buried
214 N I -2.2138 Soluble
215 H I 0.0000 Buried
216 K I -2.9026 Soluble
217 P I -1.9491 Soluble
218 S I -2.2068 Soluble
219 N I -2.7114 Soluble
220 T I -2.3096 Soluble
221 K I -2.8538 Soluble
222 V I -1.7977 Soluble
223 D I -2.5650 Soluble
224 K I -1.8731 Soluble
225 K I -2.2653 Soluble
226 V I 0.0000 Buried
227 E I -2.7732 Soluble
228 P I -1.9619 Soluble
229 K I -2.2480 Soluble
1 D M -2.2005 Soluble
2 I M -1.5312 Soluble
3 Q M -2.0736 Soluble
4 M M 0.0000 Buried
5 T M -1.2686 Soluble
6 Q M 0.0000 Buried
7 S M -0.6943 Soluble
8 P M -0.3786 Soluble
9 S M -0.6798 Soluble
10 S M -0.5945 Soluble
11 L M -0.4104 Soluble
12 S M -0.7096 Soluble
13 A M -0.9530 Soluble
14 S M -0.9155 Soluble
15 V M -0.2623 Soluble
16 G M -0.8434 Soluble
17 D M -1.9529 Soluble
18 R M -2.3775 Soluble
19 V M 0.0000 Buried
20 T M -0.6534 Soluble
21 I M 0.0000 Buried
22 T M -0.8744 Soluble
23 C M 0.0000 Buried
24 R M -2.8436 Soluble
25 A M -2.2499 Soluble
26 S M -1.9289 Soluble
27 Q M -2.1582 Soluble
28 D M -2.7593 Soluble
29 I M 0.0000 Buried
36 S M -1.1063 Soluble
37 N M -0.5825 Soluble
38 Y M 0.2480 Aggregation-prone
39 L M 0.0000 Buried
40 N M 0.0000 Buried
41 W M 0.0000 Buried
42 Y M 0.0000 Buried
43 Q M 0.0000 Buried
44 Q M 0.0000 Buried
45 K M -1.5846 Soluble
46 P M -1.3588 Soluble
47 G M -1.7407 Soluble
48 K M -2.4480 Soluble
49 A M -1.4972 Soluble
50 P M 0.0000 Buried
51 K M -1.4999 Soluble
52 L M 0.0000 Buried
53 L M 0.0000 Buried
54 I M 0.0000 Buried
55 Y M 0.1533 Aggregation-prone
56 Y M 0.2805 Aggregation-prone
57 T M 0.0000 Buried
65 S M -0.7876 Soluble
66 R M -1.4265 Soluble
67 L M -0.2973 Soluble
68 R M -0.8397 Soluble
69 S M -0.7137 Soluble
70 G M -0.5528 Soluble
71 V M -0.3513 Soluble
72 P M -0.3412 Soluble
74 S M -0.4233 Soluble
75 R M -0.7763 Soluble
76 F M 0.0000 Buried
77 S M -0.4764 Soluble
78 G M 0.0000 Buried
79 S M -0.9020 Soluble
80 G M -1.1035 Soluble
83 S M -1.2507 Soluble
84 G M -1.8903 Soluble
85 T M -2.4381 Soluble
86 D M -2.7980 Soluble
87 F M 0.0000 Buried
88 T M -0.8483 Soluble
89 L M 0.0000 Buried
90 T M -0.6024 Soluble
91 I M 0.0000 Buried
92 S M -1.2501 Soluble
93 S M -1.0826 Soluble
94 L M 0.0000 Buried
95 Q M -0.9614 Soluble
96 P M -1.6293 Soluble
97 E M -2.1842 Soluble
98 D M 0.0000 Buried
99 F M 0.0000 Buried
100 A M 0.0000 Buried
101 T M -0.6053 Soluble
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 Q M 0.0000 Buried
106 Q M 0.0000 Buried
107 G M 0.0163 Aggregation-prone
108 H M -0.6475 Soluble
109 T M -0.1385 Soluble
114 L M 0.1732 Aggregation-prone
115 P M -0.3501 Soluble
116 P M 0.0000 Buried
117 T M -0.7824 Soluble
118 F M 0.0000 Buried
119 G M 0.0000 Buried
120 Q M -1.5509 Soluble
121 G M 0.0000 Buried
122 T M 0.0000 Buried
123 K M -1.0172 Soluble
124 V M 0.0000 Buried
125 E M -1.0185 Soluble
126 I M 0.0000 Buried
127 K M -1.1529 Soluble
128 R M -0.7195 Soluble
129 T M 0.0680 Aggregation-prone
130 V M 0.8328 Aggregation-prone
131 A M 0.2854 Aggregation-prone
132 A M -0.0208 Soluble
133 P M 0.0000 Buried
134 S M -0.1269 Soluble
135 V M 0.0000 Buried
136 F M 0.0000 Buried
137 I M 0.0000 Buried
138 F M 0.0000 Buried
139 P M -0.9032 Soluble
140 P M -0.7312 Soluble
141 S M -1.5094 Soluble
142 D M -2.7565 Soluble
143 E M -2.5768 Soluble
144 Q M 0.0000 Buried
145 L M -2.1116 Soluble
146 K M -2.7314 Soluble
147 S M -1.6624 Soluble
148 G M -1.1868 Soluble
149 T M -0.9956 Soluble
150 A M 0.0000 Buried
151 S M 0.0000 Buried
152 V M 0.0000 Buried
153 V M 0.0000 Buried
154 C M 0.0000 Buried
155 L M 0.0000 Buried
156 L M 0.0000 Buried
157 N M 0.0000 Buried
158 N M -0.6065 Soluble
159 F M 0.0000 Buried
160 Y M 0.0000 Buried
161 P M -1.7094 Soluble
162 R M -3.0779 Soluble
163 E M -3.2385 Soluble
164 A M -2.3766 Soluble
165 K M -2.4493 Soluble
166 V M -1.0680 Soluble
167 Q M -0.6966 Soluble
168 W M 0.0000 Buried
169 K M -0.4934 Soluble
170 V M 0.0000 Buried
171 D M -1.5690 Soluble
172 N M -1.2212 Soluble
173 A M -0.2139 Soluble
174 L M 0.6919 Aggregation-prone
175 Q M -0.1643 Soluble
176 S M -0.4235 Soluble
177 G M -0.8717 Soluble
178 N M -0.7760 Soluble
179 S M -1.0870 Soluble
180 Q M -1.3005 Soluble
181 E M -1.9123 Soluble
182 S M -0.9851 Soluble
183 V M -0.8553 Soluble
184 T M -1.1705 Soluble
185 E M -2.2619 Soluble
186 Q M 0.0000 Buried
187 D M -2.0095 Soluble
188 S M -2.2392 Soluble
189 K M -2.5189 Soluble
190 D M -1.7337 Soluble
191 S M 0.0000 Buried
192 T M 0.0000 Buried
193 Y M 0.0000 Buried
194 S M 0.0000 Buried
195 L M 0.0000 Buried
196 S M 0.0000 Buried
197 S M 0.0000 Buried
198 T M -0.6679 Soluble
199 L M 0.0000 Buried
200 T M -0.2759 Soluble
201 L M -0.6860 Soluble
202 S M -1.1779 Soluble
203 K M -2.1291 Soluble
204 A M -1.9508 Soluble
205 D M -2.8737 Soluble
206 Y M 0.0000 Buried
207 E M -3.1347 Soluble
208 K M -3.5601 Soluble
209 H M -3.2195 Soluble
210 K M -3.4474 Soluble
211 V M -1.8918 Soluble
212 Y M 0.0000 Buried
213 A M 0.0000 Buried
214 C M 0.0000 Buried
215 E M -0.8228 Soluble
216 V M 0.0000 Buried
217 T M -1.1163 Soluble
218 H M 0.0000 Buried
219 Q M -1.4974 Soluble
220 G M -0.3322 Soluble
221 L M -0.2131 Soluble
222 S M -0.4621 Soluble
223 S M -0.4077 Soluble
224 P M -0.4860 Soluble
225 V M 0.1916 Aggregation-prone
226 T M -0.3240 Soluble
227 K M -0.6721 Soluble
228 S M -0.7586 Soluble
229 F M 0.0000 Buried
230 N M -2.6647 Soluble
231 R M -3.3994 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8577 1.6809 CSV PDB
4.5 -0.9071 1.6412 CSV PDB
5.0 -0.9637 1.6078 CSV PDB
5.5 -1.0169 1.6078 CSV PDB
6.0 -1.055 1.6078 CSV PDB
6.5 -1.0694 1.6078 CSV PDB
7.0 -1.0609 1.6078 CSV PDB
7.5 -1.0375 1.6078 CSV PDB
8.0 -1.0055 1.6078 CSV PDB
8.5 -0.9663 1.6078 CSV PDB
9.0 -0.9194 1.6078 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH