Utomilumab / 6a3w

A4DAB286_6a3w_J_K | Source PDB: 6a3w | Chains: J, K | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.551
Max Score
1.5457
Min Score
-3.5472
Total Score
-122.32
Score CDR
-0.1867
Score non-CDR
-0.6436

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGESLRISCKGSGYSFSTYWISWVRQMPGKGLEWMGKIYPGDSYTNYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARGYGIFDYWGQGTLVTVSSYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVLVIYQDKNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYTGFGSLAVFGGGTKLTVL input.pdb
Selected Chain(s) J (Heavy), K (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5472
Maximum Score 1.5457
Average Score -0.551
Total Score -122.3188

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E J -2.0640 Soluble
2 V J -1.1385 Soluble
3 Q J -1.6500 Soluble
4 L J 0.0000 Buried
5 V J -0.5727 Soluble
6 Q J 0.0000 Buried
7 S J -0.6565 Soluble
8 G J -0.2977 Soluble
9 A J 0.3777 Aggregation-prone
11 E J 0.3443 Aggregation-prone
12 V J 1.1341 Aggregation-prone
13 K J -0.9096 Soluble
14 K J -2.2834 Soluble
15 P J -2.2259 Soluble
16 G J -1.8210 Soluble
17 E J -1.7901 Soluble
18 S J -1.6538 Soluble
19 L J 0.0000 Buried
20 R J -2.1397 Soluble
21 I J 0.0000 Buried
22 S J -0.7674 Soluble
23 C J 0.0000 Buried
24 K J -1.2079 Soluble
25 G J 0.0000 Buried
26 S J -0.9237 Soluble
27 G J -1.1966 Soluble
28 Y J -0.4596 Soluble
29 S J -0.2938 Soluble
30 F J 0.0000 Buried
35 S J -0.8094 Soluble
36 T J -0.1086 Soluble
37 Y J 0.8973 Aggregation-prone
38 W J 1.0329 Aggregation-prone
39 I J 0.0000 Buried
40 S J 0.0000 Buried
41 W J 0.0000 Buried
42 V J 0.0000 Buried
43 R J -0.5558 Soluble
44 Q J -0.7873 Soluble
45 M J -0.7887 Soluble
46 P J -0.8880 Soluble
47 G J -1.4210 Soluble
48 K J -2.1973 Soluble
49 G J -1.4395 Soluble
50 L J 0.0000 Buried
51 E J -0.8033 Soluble
52 W J 0.0000 Buried
53 M J 0.0000 Buried
54 G J 0.0000 Buried
55 K J 0.0000 Buried
56 I J 0.0000 Buried
57 Y J 0.3024 Aggregation-prone
58 P J 0.0000 Buried
59 G J -1.1734 Soluble
62 D J -1.7676 Soluble
63 S J -0.7048 Soluble
64 Y J 0.7368 Aggregation-prone
65 T J 0.4008 Aggregation-prone
66 N J -0.0056 Soluble
67 Y J -0.5102 Soluble
68 S J -0.4637 Soluble
69 P J -0.8462 Soluble
70 S J -0.8818 Soluble
71 F J 0.0000 Buried
72 Q J -1.6046 Soluble
74 G J -1.5252 Soluble
75 Q J -1.8576 Soluble
76 V J 0.0000 Buried
77 T J -0.9008 Soluble
78 I J 0.0000 Buried
79 S J -0.4580 Soluble
80 A J -0.8749 Soluble
81 D J -1.5480 Soluble
82 K J -2.0413 Soluble
83 S J -0.4052 Soluble
84 I J 0.7640 Aggregation-prone
85 S J -0.5150 Soluble
86 T J 0.0000 Buried
87 A J 0.0000 Buried
88 Y J -0.7565 Soluble
89 L J 0.0000 Buried
90 Q J -1.5216 Soluble
91 W J 0.0000 Buried
92 S J -1.3075 Soluble
93 S J -1.6039 Soluble
94 L J 0.0000 Buried
95 K J -2.5333 Soluble
96 A J -1.4999 Soluble
97 S J -0.8961 Soluble
98 D J -0.9472 Soluble
99 T J -0.2475 Soluble
100 A J 0.0000 Buried
101 M J 0.2519 Aggregation-prone
102 Y J 0.0000 Buried
103 Y J 0.0000 Buried
104 C J 0.0000 Buried
105 A J 0.0000 Buried
106 R J 0.0000 Buried
107 G J 0.7127 Aggregation-prone
108 Y J 1.2263 Aggregation-prone
109 G J 0.0000 Buried
114 I J 0.8718 Aggregation-prone
115 F J 0.0000 Buried
116 D J -0.5250 Soluble
117 Y J -0.0658 Soluble
118 W J -0.2388 Soluble
119 G J 0.0000 Buried
120 Q J -1.3900 Soluble
121 G J -0.6042 Soluble
122 T J 0.0000 Buried
123 L J 0.8572 Aggregation-prone
124 V J 0.0000 Buried
125 T J 0.1035 Aggregation-prone
126 V J 0.0000 Buried
127 S J -0.9985 Soluble
128 S J -1.2535 Soluble
2 Y K -0.1747 Soluble
3 E K -1.6905 Soluble
4 L K 0.0000 Buried
5 T K -0.9475 Soluble
6 Q K 0.0000 Buried
7 P K -0.4160 Soluble
8 P K -0.6894 Soluble
9 S K -0.5796 Soluble
11 V K -0.2414 Soluble
12 S K -0.1357 Soluble
13 V K 0.0000 Buried
14 S K -0.3105 Soluble
15 P K -0.6990 Soluble
16 G K -1.4421 Soluble
17 Q K -1.7540 Soluble
18 T K -1.0101 Soluble
19 A K -0.4852 Soluble
21 I K 0.0507 Aggregation-prone
22 T K -0.0759 Soluble
23 C K 0.0000 Buried
24 S K -1.5474 Soluble
25 G K -2.1624 Soluble
26 D K -3.3333 Soluble
27 N K -3.5472 Soluble
28 I K 0.0000 Buried
29 G K -2.3945 Soluble
36 D K -2.6626 Soluble
37 Q K -0.8748 Soluble
38 Y K 0.5218 Aggregation-prone
39 A K 0.0000 Buried
40 H K 0.0000 Buried
41 W K 0.0000 Buried
42 Y K 0.0000 Buried
43 Q K -0.3795 Soluble
44 Q K 0.0000 Buried
45 K K -1.4797 Soluble
46 P K -1.4267 Soluble
47 G K -1.5127 Soluble
48 Q K -1.8571 Soluble
49 S K -0.8932 Soluble
50 P K 0.0000 Buried
51 V K 0.6488 Aggregation-prone
52 L K 0.5135 Aggregation-prone
53 V K 0.0000 Buried
54 I K 0.0000 Buried
55 Y K -0.3169 Soluble
56 Q K -0.4367 Soluble
57 D K -1.1316 Soluble
65 K K -2.1331 Soluble
66 N K -1.5939 Soluble
67 R K -1.7082 Soluble
68 P K -0.9063 Soluble
69 S K -0.8475 Soluble
70 G K -0.8376 Soluble
71 I K -0.6214 Soluble
72 P K -1.2264 Soluble
74 E K -2.2220 Soluble
75 R K -1.4507 Soluble
76 F K 0.0000 Buried
77 S K -1.2932 Soluble
78 G K -1.1767 Soluble
79 S K -1.0422 Soluble
80 N K -1.4379 Soluble
83 S K -1.5819 Soluble
84 G K -2.3687 Soluble
85 N K -3.0455 Soluble
86 T K -1.5543 Soluble
87 A K 0.0000 Buried
88 T K -0.4289 Soluble
89 L K 0.0000 Buried
90 T K -0.2012 Soluble
91 I K 0.0000 Buried
92 S K -1.1274 Soluble
93 G K -1.1475 Soluble
94 T K 0.0000 Buried
95 Q K -0.6166 Soluble
96 A K 0.2146 Aggregation-prone
97 M K 0.5592 Aggregation-prone
98 D K 0.0000 Buried
99 E K -0.7433 Soluble
100 A K 0.0000 Buried
101 D K -1.3791 Soluble
102 Y K 0.0000 Buried
103 Y K 0.0000 Buried
104 C K 0.0000 Buried
105 A K 0.0000 Buried
106 T K 0.0000 Buried
107 Y K 0.9879 Aggregation-prone
108 T K 0.6234 Aggregation-prone
109 G K 0.4128 Aggregation-prone
110 F K 1.5457 Aggregation-prone
113 G K 0.4025 Aggregation-prone
114 S K 0.4761 Aggregation-prone
115 L K 0.7107 Aggregation-prone
116 A K 0.0000 Buried
117 V K 0.0217 Aggregation-prone
118 F K 0.0000 Buried
119 G K 0.0000 Buried
120 G K -1.2143 Soluble
121 G K -1.0034 Soluble
122 T K 0.0000 Buried
123 K K -1.2604 Soluble
124 L K 0.0000 Buried
125 T K -0.1721 Soluble
126 V K 0.0000 Buried
127 L K 1.2517 Aggregation-prone

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.4116 4.2142 CSV PDB
4.5 -0.448 4.2142 CSV PDB
5.0 -0.4892 4.2142 CSV PDB
5.5 -0.5271 4.2142 CSV PDB
6.0 -0.5526 4.2142 CSV PDB
6.5 -0.5598 4.2142 CSV PDB
7.0 -0.5502 4.2142 CSV PDB
7.5 -0.5299 4.2142 CSV PDB
8.0 -0.5031 4.2142 CSV PDB
8.5 -0.4706 4.2142 CSV PDB
9.0 -0.4321 4.2142 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH