Basiliximab / 1mim

A4DAB035_1mim_H_L | Source PDB: 1mim | Chains: H, L | Done
99.1
Yes
10 Å
Yes
Avg A4D Score
-0.6748
Max Score
1.4407
Min Score
-3.5414
Total Score
-286.77
Score CDR
-0.3798
Score non-CDR
-0.7062

Calculation Parameters

Chain Sequence(s) QLQQSGTVLARPGASVKMSCKASGYSFTRYWMHWIKQRPGQGLEWIGAIYPGNSDTSYNQKFEGKAKLTAVTSASTAYMELSSLTHEDSAVYYCSRDYGYYFDFWGQGTTLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPQIVSTQSPAIMSASPGEKVTMTCSASSSRSYMQWYQQKPGTSPKRWIYDTSKLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYCHQRSSYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5414
Maximum Score 1.4407
Average Score -0.6748
Total Score -286.7693

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
3 Q H -1.4794 Soluble
4 L H 0.0000 Buried
5 Q H -1.5784 Soluble
6 Q H 0.0000 Buried
7 S H -0.8950 Soluble
8 G H -0.6039 Soluble
9 T H -0.0950 Soluble
11 V H 0.2342 Aggregation-prone
12 L H 0.3636 Aggregation-prone
13 A H -0.5622 Soluble
14 R H -1.8404 Soluble
15 P H -1.6591 Soluble
16 G H -1.1911 Soluble
17 A H -0.8370 Soluble
18 S H -1.0021 Soluble
19 V H -0.8639 Soluble
20 K H -1.9211 Soluble
21 M H 0.0000 Buried
22 S H -0.7805 Soluble
23 C H 0.0000 Buried
24 K H -1.0868 Soluble
25 A H 0.0000 Buried
26 S H -0.9623 Soluble
27 G H -0.7126 Soluble
28 Y H -0.5092 Soluble
29 S H -0.7237 Soluble
30 F H 0.0000 Buried
35 T H -1.1938 Soluble
36 R H -1.7501 Soluble
37 Y H -0.3618 Soluble
38 W H -0.1934 Soluble
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 I H 0.0000 Buried
43 K H -0.5888 Soluble
44 Q H -0.9147 Soluble
45 R H -1.5448 Soluble
46 P H -1.1136 Soluble
47 G H -1.4172 Soluble
48 Q H -1.9625 Soluble
49 G H -1.2733 Soluble
50 L H 0.0000 Buried
51 E H -1.0488 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 A H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.9770 Soluble
58 P H 0.0000 Buried
59 G H -1.5628 Soluble
62 N H -2.1672 Soluble
63 S H -1.4988 Soluble
64 D H -2.2370 Soluble
65 T H -1.2027 Soluble
66 S H -0.6745 Soluble
67 Y H -1.2278 Soluble
68 N H -1.7327 Soluble
69 Q H -2.7172 Soluble
70 K H -2.9750 Soluble
71 F H 0.0000 Buried
72 E H -3.3004 Soluble
74 G H -2.2498 Soluble
75 K H -2.1010 Soluble
76 A H 0.0000 Buried
77 K H -2.4718 Soluble
78 L H 0.0000 Buried
79 T H -0.3565 Soluble
80 A H 0.3683 Aggregation-prone
81 V H 1.1364 Aggregation-prone
82 T H 0.2666 Aggregation-prone
83 S H 0.1363 Aggregation-prone
84 A H 0.1485 Aggregation-prone
85 S H -0.0667 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.4655 Soluble
89 M H 0.0000 Buried
90 E H -1.7753 Soluble
91 L H 0.0000 Buried
92 S H -1.0855 Soluble
93 S H -0.9164 Soluble
94 L H 0.0000 Buried
95 T H -1.5956 Soluble
96 H H -2.2490 Soluble
97 E H -2.4212 Soluble
98 D H 0.0000 Buried
99 S H -0.9068 Soluble
100 A H 0.0000 Buried
101 V H -0.0450 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 S H 0.0000 Buried
106 R H 0.0000 Buried
107 D H 0.5648 Aggregation-prone
108 Y H 1.0191 Aggregation-prone
109 G H 0.3636 Aggregation-prone
113 Y H 0.5659 Aggregation-prone
114 Y H 0.4596 Aggregation-prone
115 F H 0.0000 Buried
116 D H 0.0000 Buried
117 F H 0.8300 Aggregation-prone
118 W H -0.0877 Soluble
119 G H 0.0000 Buried
120 Q H -1.4159 Soluble
121 G H -0.7749 Soluble
122 T H 0.0000 Buried
123 T H -0.1055 Soluble
124 L H 0.0000 Buried
125 T H -0.4091 Soluble
126 V H 0.0000 Buried
127 S H -0.9640 Soluble
128 S H -0.9366 Soluble
129 A H -0.5336 Soluble
130 S H -0.6716 Soluble
131 T H -0.5989 Soluble
132 K H -1.0352 Soluble
133 G H -1.4044 Soluble
134 P H 0.0000 Buried
135 S H -0.3353 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0970 Soluble
139 L H 0.0000 Buried
140 A H -0.8216 Soluble
141 P H 0.0000 Buried
142 S H -0.2986 Soluble
143 S H -0.6514 Soluble
144 K H -1.2644 Soluble
145 S H -0.8371 Soluble
146 T H -0.7592 Soluble
147 S H -0.8833 Soluble
148 G H -1.1806 Soluble
149 G H -1.0039 Soluble
150 T H -0.7575 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.4138 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.6847 Soluble
164 P H -0.8341 Soluble
165 V H -0.7170 Soluble
166 T H -0.6092 Soluble
167 V H -0.4164 Soluble
168 S H -0.3816 Soluble
169 W H 0.0000 Buried
170 N H -0.8485 Soluble
171 S H -0.7174 Soluble
172 G H -0.5800 Soluble
173 A H -0.2405 Soluble
174 L H 0.0398 Aggregation-prone
175 T H -0.1087 Soluble
176 S H -0.1167 Soluble
177 G H -0.0500 Soluble
178 V H 0.2960 Aggregation-prone
179 H H -0.1125 Soluble
180 T H 0.0284 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3487 Soluble
183 A H 0.1523 Aggregation-prone
184 V H 0.4830 Aggregation-prone
185 L H 1.0102 Aggregation-prone
186 Q H 0.2218 Aggregation-prone
187 S H -0.0805 Soluble
188 S H -0.1911 Soluble
189 G H 0.0518 Aggregation-prone
190 L H 0.0867 Aggregation-prone
191 Y H 0.3775 Aggregation-prone
192 S H 0.2455 Aggregation-prone
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.0770 Soluble
199 V H 0.0000 Buried
200 P H -0.5958 Soluble
201 S H -0.7281 Soluble
202 S H -0.6338 Soluble
203 S H -0.6993 Soluble
204 L H -0.9367 Soluble
205 G H -1.0579 Soluble
206 T H -0.8840 Soluble
207 Q H -1.5381 Soluble
208 T H -1.4981 Soluble
209 Y H 0.0000 Buried
210 I H -1.5397 Soluble
211 C H 0.0000 Buried
212 N H -1.5754 Soluble
213 V H 0.0000 Buried
214 N H -1.8799 Soluble
215 H H 0.0000 Buried
216 K H -2.7453 Soluble
217 P H -1.5252 Soluble
218 S H -1.7777 Soluble
219 N H -2.4558 Soluble
220 T H -2.0176 Soluble
221 K H -2.5868 Soluble
222 V H -1.4683 Soluble
223 D H -2.5679 Soluble
224 K H -2.1778 Soluble
225 R H -2.8740 Soluble
226 V H 0.0000 Buried
227 E H -2.6329 Soluble
228 P H -1.3691 Soluble
1 Q L -0.7351 Soluble
2 I L 0.0000 Buried
3 V L 1.3290 Aggregation-prone
4 S L 0.4939 Aggregation-prone
5 T L 0.1388 Aggregation-prone
6 Q L 0.0000 Buried
7 S L -0.0811 Soluble
8 P L 0.2273 Aggregation-prone
9 A L 0.5124 Aggregation-prone
10 I L 1.4407 Aggregation-prone
11 M L 0.3946 Aggregation-prone
12 S L -0.6577 Soluble
13 A L 0.0000 Buried
14 S L -1.8084 Soluble
15 P L -2.0254 Soluble
16 G L -1.8798 Soluble
17 E L -2.6186 Soluble
18 K L -2.6545 Soluble
19 V L 0.0000 Buried
20 T L -0.5116 Soluble
21 M L 0.0000 Buried
22 T L -0.2455 Soluble
23 C L 0.0000 Buried
24 S L -0.1974 Soluble
25 A L 0.0000 Buried
26 S L -0.1252 Soluble
27 S L -0.4999 Soluble
28 S L -0.8134 Soluble
29 R L -0.9827 Soluble
37 S L -0.4590 Soluble
38 Y L 0.2827 Aggregation-prone
39 M L 0.0000 Buried
40 Q L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.0741 Soluble
45 K L -1.5243 Soluble
46 P L -1.1737 Soluble
47 G L -0.9968 Soluble
48 T L -0.9681 Soluble
49 S L -0.7319 Soluble
50 P L 0.0000 Buried
51 K L -0.9873 Soluble
52 R L -0.7782 Soluble
53 W L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.1612 Soluble
56 D L -0.2111 Soluble
57 T L -0.3664 Soluble
65 S L -0.7528 Soluble
66 K L -1.3510 Soluble
67 L L -0.5963 Soluble
68 A L -0.5901 Soluble
69 S L -0.6164 Soluble
70 G L -0.5966 Soluble
71 V L 0.0000 Buried
72 P L -0.2936 Soluble
74 A L -0.1956 Soluble
75 R L -0.7480 Soluble
76 F L 0.0000 Buried
77 S L -0.4480 Soluble
78 G L 0.0000 Buried
79 S L -0.6964 Soluble
80 G L -0.5837 Soluble
83 S L -0.6427 Soluble
84 G L -0.7415 Soluble
85 T L -0.7144 Soluble
86 S L -0.6480 Soluble
87 Y L 0.0000 Buried
88 S L -0.3394 Soluble
89 L L 0.0000 Buried
90 T L -0.5782 Soluble
91 I L 0.0000 Buried
92 S L -1.4313 Soluble
93 S L -1.7477 Soluble
94 M L 0.0000 Buried
95 E L -2.4329 Soluble
96 A L -2.0212 Soluble
97 E L -2.3623 Soluble
98 D L 0.0000 Buried
99 A L -1.3815 Soluble
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 H L 0.0000 Buried
106 Q L 0.0000 Buried
107 R L -0.0499 Soluble
108 S L -0.4104 Soluble
115 S L -0.1698 Soluble
116 Y L 0.0521 Aggregation-prone
117 T L 0.0956 Aggregation-prone
118 F L 0.2596 Aggregation-prone
119 G L 0.0000 Buried
120 G L -1.0223 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.7948 Soluble
124 L L 0.0000 Buried
125 E L -1.2460 Soluble
126 I L -1.6738 Soluble
127 K L -1.8705 Soluble
128 R L -1.4063 Soluble
129 T L -0.2461 Soluble
130 V L 0.0953 Aggregation-prone
131 A L 0.0489 Aggregation-prone
132 A L -0.1537 Soluble
133 P L 0.0000 Buried
134 S L -0.2262 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.4790 Soluble
140 P L -0.7640 Soluble
141 S L -1.3934 Soluble
142 D L -2.6330 Soluble
143 E L -2.3097 Soluble
144 Q L 0.0000 Buried
145 L L -1.9879 Soluble
146 K L -2.5690 Soluble
147 S L -1.5857 Soluble
148 G L -1.2761 Soluble
149 T L -0.8433 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -1.1528 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.9274 Soluble
162 R L -3.1645 Soluble
163 E L -3.2726 Soluble
164 A L -2.3951 Soluble
165 K L -2.4809 Soluble
166 V L -1.3087 Soluble
167 Q L -0.7433 Soluble
168 W L 0.0000 Buried
169 K L -0.7675 Soluble
170 V L 0.0000 Buried
171 D L -1.8116 Soluble
172 N L -1.6333 Soluble
173 A L -0.4687 Soluble
174 L L 0.0976 Aggregation-prone
175 Q L -0.5731 Soluble
176 S L -0.6226 Soluble
177 G L -0.9490 Soluble
178 N L -0.8775 Soluble
179 S L -1.1566 Soluble
180 Q L -1.4049 Soluble
181 E L -1.9030 Soluble
182 S L -1.0137 Soluble
183 V L -0.9183 Soluble
184 T L -1.3103 Soluble
185 E L -2.4361 Soluble
186 Q L -2.3988 Soluble
187 D L -2.6246 Soluble
188 S L -2.4837 Soluble
189 K L -2.9126 Soluble
190 D L -3.0725 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6879 Soluble
199 L L 0.0000 Buried
200 T L -0.3962 Soluble
201 L L -0.6037 Soluble
202 S L -0.9399 Soluble
203 K L -1.8948 Soluble
204 A L -1.7615 Soluble
205 D L -2.4355 Soluble
206 Y L 0.0000 Buried
207 E L -3.4765 Soluble
208 K L -3.5414 Soluble
209 H L -3.0700 Soluble
210 K L -3.3054 Soluble
211 V L -1.7120 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6592 Soluble
216 V L 0.0000 Buried
217 T L -1.1725 Soluble
218 H L 0.0000 Buried
219 Q L -1.7656 Soluble
220 G L -0.4788 Soluble
221 L L -0.2351 Soluble
222 S L -0.4337 Soluble
223 S L -0.3642 Soluble
224 P L -0.4677 Soluble
225 V L 0.1410 Aggregation-prone
226 T L -0.3825 Soluble
227 K L -0.5906 Soluble
228 S L -0.5722 Soluble
229 F L -0.9773 Soluble
230 N L -2.0289 Soluble
231 R L -2.6315 Soluble
232 G L -2.2969 Soluble
233 E L -2.5080 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7448 2.182 CSV PDB
4.5 -0.7833 2.1669 CSV PDB
5.0 -0.8298 2.1549 CSV PDB
5.5 -0.8743 2.1525 CSV PDB
6.0 -0.9061 2.1576 CSV PDB
6.5 -0.9152 2.1783 CSV PDB
7.0 -0.8992 2.2664 CSV PDB
7.5 -0.8656 2.3869 CSV PDB
8.0 -0.8226 2.5223 CSV PDB
8.5 -0.774 2.663 CSV PDB
9.0 -0.7206 2.8049 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH