Amubarvimab / 7swo

A4DAB014_7swo_H_L | Source PDB: 7swo | Chains: H, L | Done
95.09
Yes
10 Å
Yes
Avg A4D Score
-0.6662
Max Score
2.5201
Min Score
-3.7008
Total Score
-287.79
Score CDR
0.0434
Score non-CDR
-0.7466

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGITVSSNYMSWVRQAPGKGLEWVSVIYSGGSTFYADSVKGRFTISRDNSKNTLYLQMSSLRAEDTAVYYCARDLVVFGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSIVLTQSPGTLSLSPGETATLSCRASQSVGSSYLTWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQHYGSSPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7008
Maximum Score 2.5201
Average Score -0.6662
Total Score -287.7859

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.2622 Soluble
2 V H 0.4318 Aggregation-prone
3 Q H -0.4487 Soluble
4 L H 0.0000 Buried
5 V H 0.6536 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5827 Soluble
8 G H -0.9671 Soluble
9 G H -0.6906 Soluble
11 G H -0.2473 Soluble
12 L H 0.5205 Aggregation-prone
13 V H -0.2414 Soluble
14 Q H -1.3175 Soluble
15 P H -1.2707 Soluble
16 G H -1.2834 Soluble
17 G H -0.8401 Soluble
18 S H -1.1367 Soluble
19 L H -1.0185 Soluble
20 R H -1.8625 Soluble
21 L H 0.0000 Buried
22 S H -0.4163 Soluble
23 C H 0.0000 Buried
24 A H -0.1518 Soluble
25 A H -0.1000 Soluble
26 S H -0.1592 Soluble
27 G H -0.4174 Soluble
28 I H -0.0545 Soluble
29 T H -0.6814 Soluble
30 V H 0.0000 Buried
35 S H -0.8689 Soluble
36 S H -0.4590 Soluble
37 N H -0.1023 Soluble
38 Y H 0.3134 Aggregation-prone
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.8077 Soluble
44 Q H -1.1535 Soluble
45 A H -1.4833 Soluble
46 P H -1.1012 Soluble
47 G H -1.5368 Soluble
48 K H -2.5564 Soluble
49 G H -1.9993 Soluble
50 L H 0.0000 Buried
51 E H -1.8117 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H 0.2170 Aggregation-prone
58 S H -0.3645 Soluble
59 G H -0.7256 Soluble
63 G H -0.7292 Soluble
64 S H -0.0791 Soluble
65 T H 0.5109 Aggregation-prone
66 F H 0.7739 Aggregation-prone
67 Y H -0.5320 Soluble
68 A H -1.2988 Soluble
69 D H -2.3343 Soluble
70 S H -1.9796 Soluble
71 V H 0.0000 Buried
72 K H -2.9326 Soluble
74 G H -2.3137 Soluble
75 R H -2.8613 Soluble
76 F H 0.0000 Buried
77 T H -0.9284 Soluble
78 I H 0.0000 Buried
79 S H -0.5854 Soluble
80 R H -1.2422 Soluble
81 D H -1.6865 Soluble
82 N H -2.2977 Soluble
83 S H -1.8746 Soluble
84 K H -2.4518 Soluble
85 N H -1.8609 Soluble
86 T H -1.0239 Soluble
87 L H 0.0000 Buried
88 Y H -0.5081 Soluble
89 L H 0.0000 Buried
90 Q H -1.4217 Soluble
91 M H 0.0000 Buried
92 S H -1.4036 Soluble
93 S H -1.3197 Soluble
94 L H 0.0000 Buried
95 R H -1.5262 Soluble
96 A H -1.3323 Soluble
97 E H -1.9615 Soluble
98 D H 0.0000 Buried
99 T H 0.0000 Buried
100 A H 0.0000 Buried
101 V H -0.0518 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.3517 Soluble
107 D H 0.0000 Buried
108 L H 1.3744 Aggregation-prone
109 V H 1.8173 Aggregation-prone
110 V H 2.5201 Aggregation-prone
113 F H 1.9879 Aggregation-prone
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.8112 Soluble
117 V H -0.5798 Soluble
118 W H -0.8839 Soluble
119 G H 0.0000 Buried
120 Q H -1.3410 Soluble
121 G H -0.8065 Soluble
122 T H -0.5037 Soluble
123 T H -0.1579 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.6680 Soluble
128 S H -0.7525 Soluble
129 A H -0.5109 Soluble
130 S H -0.5133 Soluble
131 T H -0.4777 Soluble
132 K H -1.0806 Soluble
133 G H -1.2805 Soluble
134 P H -0.4700 Soluble
135 S H -0.1274 Soluble
136 V H -0.2863 Soluble
137 F H -0.4932 Soluble
138 P H -1.0135 Soluble
139 L H 0.0000 Buried
140 A H -0.9456 Soluble
141 P H 0.0000 Buried
142 S H -0.5465 Soluble
143 S H -0.5206 Soluble
144 K H -0.8559 Soluble
145 S H 0.0000 Buried
146 T H -0.5800 Soluble
147 S H -0.6900 Soluble
148 G H -0.7050 Soluble
149 G H -0.5680 Soluble
150 T H -0.3612 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.2246 Soluble
159 D H -0.3632 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.7612 Soluble
164 P H -0.9378 Soluble
165 V H -0.5453 Soluble
166 T H -0.5111 Soluble
167 V H -0.0099 Soluble
168 S H -0.2610 Soluble
169 W H 0.0000 Buried
170 N H -0.8789 Soluble
171 S H -0.7348 Soluble
172 G H -0.4806 Soluble
173 A H -0.1774 Soluble
174 L H 0.0599 Aggregation-prone
175 T H -0.0675 Soluble
176 S H -0.1574 Soluble
177 G H -0.1692 Soluble
178 V H 0.1310 Aggregation-prone
179 H H -0.3758 Soluble
180 T H -0.2248 Soluble
181 F H 0.0000 Buried
182 P H -0.3633 Soluble
183 A H 0.2425 Aggregation-prone
184 V H 0.4848 Aggregation-prone
185 L H 1.0493 Aggregation-prone
186 Q H 0.2461 Aggregation-prone
187 S H -0.1422 Soluble
188 S H -0.1588 Soluble
189 G H 0.1269 Aggregation-prone
190 L H 0.1764 Aggregation-prone
191 Y H 0.0000 Buried
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H -0.0032 Soluble
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.0603 Soluble
199 V H -0.0138 Soluble
200 P H -0.4638 Soluble
201 S H -0.5696 Soluble
202 S H -0.5984 Soluble
203 S H -0.6367 Soluble
204 L H -0.7246 Soluble
205 G H -1.0644 Soluble
206 T H -1.1363 Soluble
207 Q H -1.5445 Soluble
208 T H -1.0509 Soluble
209 Y H 0.0000 Buried
210 I H -1.2273 Soluble
211 C H 0.0000 Buried
212 N H 0.0000 Buried
213 V H 0.0000 Buried
214 N H -1.6676 Soluble
215 H H 0.0000 Buried
216 K H -2.7510 Soluble
217 P H -1.5377 Soluble
218 S H -1.7665 Soluble
219 N H -2.5749 Soluble
220 T H -2.0676 Soluble
221 K H -2.5289 Soluble
222 V H -1.3108 Soluble
223 D H -1.8658 Soluble
224 K H -1.8023 Soluble
225 R H -1.8974 Soluble
226 V H 0.0000 Buried
227 E H -2.7113 Soluble
228 P H -1.8616 Soluble
229 K H -1.8732 Soluble
230 S H -1.0001 Soluble
2 I L 2.0908 Aggregation-prone
3 V L 2.0476 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.4614 Soluble
6 Q L 0.0000 Buried
7 S L -0.9575 Soluble
8 P L -0.6573 Soluble
9 G L -0.8023 Soluble
10 T L -0.6032 Soluble
11 L L -0.2422 Soluble
12 S L -0.6738 Soluble
13 L L -0.9625 Soluble
14 S L -1.6502 Soluble
15 P L -1.6662 Soluble
16 G L -2.0585 Soluble
17 E L -2.3294 Soluble
18 T L -1.4299 Soluble
19 A L 0.0000 Buried
20 T L -0.1398 Soluble
21 L L 0.0000 Buried
22 S L -0.8932 Soluble
23 C L 0.0000 Buried
24 R L -2.1612 Soluble
25 A L 0.0000 Buried
26 S L -0.2827 Soluble
27 Q L -1.0327 Soluble
28 S L -1.1474 Soluble
29 V L -0.5492 Soluble
30 G L -0.5222 Soluble
36 S L -0.2782 Soluble
37 S L 0.5978 Aggregation-prone
38 Y L 1.5169 Aggregation-prone
39 L L 0.0000 Buried
40 T L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.4126 Soluble
46 P L -1.3483 Soluble
47 G L -1.3741 Soluble
48 Q L -2.0112 Soluble
49 A L -1.4283 Soluble
50 P L 0.0000 Buried
51 R L -1.8932 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.7482 Aggregation-prone
56 G L 0.6754 Aggregation-prone
57 A L -0.1072 Soluble
65 S L -0.5394 Soluble
66 S L -0.4821 Soluble
67 R L -1.3629 Soluble
68 A L -0.6762 Soluble
69 T L -0.5455 Soluble
70 G L -0.7694 Soluble
71 I L -0.8422 Soluble
72 P L -1.3079 Soluble
74 D L -2.2497 Soluble
75 R L -2.0223 Soluble
76 F L 0.0000 Buried
77 S L -0.8342 Soluble
78 G L -0.4788 Soluble
79 S L -0.5852 Soluble
80 G L -0.9478 Soluble
83 S L -1.1220 Soluble
84 G L -1.1585 Soluble
85 T L -1.5477 Soluble
86 D L -2.1137 Soluble
87 F L 0.0000 Buried
88 T L -0.7354 Soluble
89 L L 0.0000 Buried
90 T L -0.4370 Soluble
91 I L 0.0000 Buried
92 S L -1.9832 Soluble
93 R L -2.9127 Soluble
94 L L 0.0000 Buried
95 E L -2.9731 Soluble
96 P L -1.9301 Soluble
97 E L -2.4632 Soluble
98 D L 0.0000 Buried
99 F L 0.0000 Buried
100 A L 0.0000 Buried
101 V L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 H L 0.0000 Buried
107 Y L 0.0000 Buried
108 G L 0.2866 Aggregation-prone
114 S L 0.0610 Aggregation-prone
115 S L 0.1015 Aggregation-prone
116 P L 0.0000 Buried
117 T L 0.3318 Aggregation-prone
118 F L -0.1738 Soluble
119 G L 0.0000 Buried
120 Q L -1.8320 Soluble
121 G L -1.1148 Soluble
122 T L 0.0000 Buried
123 K L -0.8961 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -1.1651 Soluble
127 K L -1.8137 Soluble
128 R L -1.5560 Soluble
129 T L -0.1365 Soluble
130 V L 0.8916 Aggregation-prone
131 A L 0.3976 Aggregation-prone
132 A L 0.1468 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.1191 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.2705 Soluble
140 P L 0.0000 Buried
141 S L -1.9850 Soluble
142 D L -3.2892 Soluble
143 E L -3.1315 Soluble
144 Q L 0.0000 Buried
145 L L -2.5920 Soluble
146 K L -3.0319 Soluble
147 S L -1.9261 Soluble
148 G L -1.5670 Soluble
149 T L -0.9307 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.3219 Soluble
158 N L -0.7670 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.8879 Soluble
162 R L -2.9768 Soluble
163 E L -3.1714 Soluble
164 A L -2.1129 Soluble
165 K L -1.6442 Soluble
166 V L -0.6118 Soluble
167 Q L -0.6125 Soluble
168 W L 0.0000 Buried
169 K L -1.0760 Soluble
170 V L 0.0000 Buried
171 D L -2.5252 Soluble
172 N L -1.8813 Soluble
173 A L -0.6815 Soluble
174 L L 0.5749 Aggregation-prone
175 Q L -0.3960 Soluble
176 S L -0.3253 Soluble
177 G L -0.7158 Soluble
178 N L -0.7593 Soluble
179 S L -1.1516 Soluble
180 Q L -1.1526 Soluble
181 E L -1.7782 Soluble
182 S L -0.8905 Soluble
183 V L -0.6988 Soluble
184 T L -0.9718 Soluble
185 E L -2.0797 Soluble
186 Q L -2.1638 Soluble
187 D L -2.2422 Soluble
188 S L -1.9865 Soluble
189 K L -2.5733 Soluble
190 D L -2.4029 Soluble
191 S L 0.0000 Buried
192 T L -1.7347 Soluble
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5900 Soluble
199 L L 0.0000 Buried
200 T L -0.3742 Soluble
201 L L -0.6850 Soluble
202 S L -1.2487 Soluble
203 K L -2.3773 Soluble
204 A L -1.9388 Soluble
205 D L -2.2479 Soluble
206 Y L 0.0000 Buried
207 E L -3.5578 Soluble
208 K L -3.5367 Soluble
209 H L -3.2465 Soluble
210 K L -3.7008 Soluble
211 V L -2.2888 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L 0.0000 Buried
216 V L 0.0000 Buried
217 T L -0.7246 Soluble
218 H L 0.0000 Buried
219 Q L -1.6429 Soluble
220 G L -0.2997 Soluble
221 L L -0.0044 Soluble
222 S L -0.3811 Soluble
223 S L -0.2797 Soluble
224 P L -0.1129 Soluble
225 V L 0.4981 Aggregation-prone
226 T L -0.0735 Soluble
227 K L -0.5399 Soluble
228 S L -0.8174 Soluble
229 F L 0.0000 Buried
230 N L -2.8268 Soluble
231 R L -3.5132 Soluble
232 G L -2.5061 Soluble
233 E L -2.6085 Soluble
234 C L -0.9517 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6457 3.1891 CSV PDB
4.5 -0.6865 3.1716 CSV PDB
5.0 -0.7363 3.1426 CSV PDB
5.5 -0.7847 3.1055 CSV PDB
6.0 -0.8206 3.0649 CSV PDB
6.5 -0.8358 3.0229 CSV PDB
7.0 -0.8304 2.9806 CSV PDB
7.5 -0.8113 2.9381 CSV PDB
8.0 -0.7848 2.8959 CSV PDB
8.5 -0.7534 2.8813 CSV PDB
9.0 -0.7171 2.8801 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH