Garadacimab / 8r8d

A4DAB125_8r8d_H_L | Source PDB: 8r8d | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.5762
Max Score
2.8896
Min Score
-2.8949
Total Score
-135.98
Score CDR
—
Score non-CDR
—

Calculation Parameters

Chain Sequence(s) VQLLESGGGLVQPGGSLRLSCAASGFTFSKYIMQWVRQAPGKGLEWVSGIDIPTKGTVYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARALPRSGYLISPHYYYYALDVWGQGTTVTVSSSVLTQPPSASGTPGQRVTISCSGSSSNIGRNYVYWYQQLPGTAPKLLIYSNNQRPSGVPDRFSGSKSGTSASLAISGLRSEDEADYYCAAWDASLRGVFGGGTKLTVL input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -2.8949
Maximum Score 2.8896
Average Score -0.5762
Total Score -135.9794

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 1.2643 Aggregation-prone
3 Q H 0.1520 Aggregation-prone
4 L H 0.0000 Buried
5 L H 0.3909 Aggregation-prone
6 E H -0.2200 Soluble
7 S H -0.7434 Soluble
8 G H -1.0976 Soluble
9 G H -0.5428 Soluble
11 G H 0.3665 Aggregation-prone
12 L H 1.0744 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.5036 Soluble
15 P H -1.8526 Soluble
16 G H -1.7095 Soluble
17 G H -1.2773 Soluble
18 S H -1.4023 Soluble
19 L H -1.0053 Soluble
20 R H -2.1883 Soluble
21 L H 0.0000 Buried
22 S H -0.6272 Soluble
23 C H 0.0000 Buried
24 A H -0.3020 Soluble
25 A H 0.0000 Buried
26 S H 0.0406 Aggregation-prone
27 G H 0.1573 Aggregation-prone
28 F H 0.0644 Aggregation-prone
29 T H -0.4810 Soluble
30 F H 0.0000 Buried
35 S H -1.4607 Soluble
36 K H -1.6528 Soluble
37 Y H -0.7701 Soluble
38 I H 0.0000 Buried
39 M H 0.0000 Buried
40 Q H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8285 Soluble
45 A H -1.2417 Soluble
46 P H -1.0218 Soluble
47 G H -1.4640 Soluble
48 K H -2.2578 Soluble
49 G H -1.4108 Soluble
50 L H 0.0000 Buried
51 E H -0.8884 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 G H 0.0000 Buried
56 I H -0.9101 Soluble
57 D H -2.1756 Soluble
58 I H 0.0000 Buried
59 P H -1.1121 Soluble
62 T H -0.9243 Soluble
63 K H -1.6261 Soluble
64 G H -1.4836 Soluble
65 T H -0.9530 Soluble
66 V H 0.0000 Buried
67 Y H -1.0124 Soluble
68 A H -1.5505 Soluble
69 D H -2.5405 Soluble
70 S H -1.7547 Soluble
71 V H 0.0000 Buried
72 K H -2.6479 Soluble
74 G H -1.8055 Soluble
75 R H -1.4103 Soluble
76 F H 0.0000 Buried
77 T H -0.9055 Soluble
78 I H 0.0000 Buried
79 S H -0.6411 Soluble
80 R H -1.3239 Soluble
81 D H -1.8143 Soluble
82 N H -2.1244 Soluble
83 S H -1.8265 Soluble
84 K H -2.4809 Soluble
85 N H -1.8683 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.3464 Soluble
91 M H 0.0000 Buried
92 N H -1.6449 Soluble
93 S H -1.5279 Soluble
94 L H 0.0000 Buried
95 R H -2.8949 Soluble
96 A H -2.0877 Soluble
97 E H -2.3721 Soluble
98 D H 0.0000 Buried
99 T H -0.7721 Soluble
100 A H 0.0000 Buried
101 V H -0.0920 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 A H 0.0000 Buried
108 L H 0.0483 Aggregation-prone
109 P H -0.6251 Soluble
110 R H -0.0407 Soluble
111 S H 0.0024 Aggregation-prone
111A G H 0.6872 Aggregation-prone
111B Y H 2.1509 Aggregation-prone
111C L H 2.8649 Aggregation-prone
111D I H 2.8896 Aggregation-prone
112E S H 1.4806 Aggregation-prone
112D P H 0.0765 Aggregation-prone
112C H H -0.1659 Soluble
112B Y H 1.1515 Aggregation-prone
112A Y H 0.5864 Aggregation-prone
112 Y H 0.6499 Aggregation-prone
113 Y H 0.3302 Aggregation-prone
114 A H 0.0000 Buried
115 L H 0.0000 Buried
116 D H -0.4508 Soluble
117 V H -0.0236 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.1880 Soluble
121 G H -0.6535 Soluble
122 T H -0.4380 Soluble
123 T H -0.0559 Soluble
124 V H 0.0000 Buried
125 T H 0.0427 Aggregation-prone
126 V H 0.0000 Buried
127 S H -0.8488 Soluble
128 S H -0.5803 Soluble
2 S L 0.5232 Aggregation-prone
3 V L 1.5477 Aggregation-prone
4 L L 0.0000 Buried
5 T L 0.0017 Aggregation-prone
6 Q L -0.5025 Soluble
7 P L -0.7261 Soluble
8 P L -0.9833 Soluble
9 S L -1.2108 Soluble
11 A L -0.8581 Soluble
12 S L -0.6019 Soluble
13 G L 0.0000 Buried
14 T L -0.5179 Soluble
15 P L -1.3947 Soluble
16 G L -1.9692 Soluble
17 Q L -2.5553 Soluble
18 R L -2.8345 Soluble
19 V L 0.0000 Buried
20 T L -0.5862 Soluble
21 I L 0.0000 Buried
22 S L -0.3608 Soluble
23 C L 0.0000 Buried
24 S L -0.2277 Soluble
25 G L -0.1297 Soluble
26 S L -0.5402 Soluble
27 S L -1.1864 Soluble
28 S L -0.8705 Soluble
29 N L 0.0000 Buried
30 I L 0.0000 Buried
35 G L -1.7222 Soluble
36 R L -2.2979 Soluble
37 N L -1.3100 Soluble
38 Y L -0.3645 Soluble
39 V L 0.0000 Buried
40 Y L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 L L -0.4062 Soluble
46 P L -0.4009 Soluble
47 G L -0.6238 Soluble
48 T L -0.6281 Soluble
49 A L -0.6910 Soluble
50 P L 0.0000 Buried
51 K L -1.3541 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0000 Buried
56 S L -0.7449 Soluble
57 N L -1.2646 Soluble
65 N L -2.0174 Soluble
66 Q L -1.9722 Soluble
67 R L -1.8299 Soluble
68 P L 0.0000 Buried
69 S L -1.0249 Soluble
70 G L -0.8578 Soluble
71 V L -0.9822 Soluble
72 P L -1.3159 Soluble
74 D L -2.3179 Soluble
75 R L -1.5631 Soluble
76 F L 0.0000 Buried
77 S L -1.2957 Soluble
78 G L -1.1891 Soluble
79 S L -1.1432 Soluble
80 K L -1.1331 Soluble
83 S L -0.8484 Soluble
84 G L -1.2720 Soluble
85 T L -1.1183 Soluble
86 S L -0.7256 Soluble
87 A L 0.0000 Buried
88 S L -0.6020 Soluble
89 L L 0.0000 Buried
90 A L -0.6688 Soluble
91 I L 0.0000 Buried
92 S L -1.9835 Soluble
93 G L -1.9706 Soluble
94 L L 0.0000 Buried
95 R L -2.7195 Soluble
96 S L -1.8721 Soluble
97 E L -2.6574 Soluble
98 D L 0.0000 Buried
99 E L -2.0470 Soluble
100 A L 0.0000 Buried
101 D L -1.1511 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 A L 0.0000 Buried
106 A L 0.0000 Buried
107 W L 0.0000 Buried
108 D L -0.4711 Soluble
109 A L -0.4762 Soluble
113 S L -0.2979 Soluble
114 L L -0.2365 Soluble
115 R L -0.6219 Soluble
116 G L -0.2286 Soluble
117 V L 0.2977 Aggregation-prone
118 F L 0.2321 Aggregation-prone
119 G L 0.0000 Buried
120 G L -0.6903 Soluble
121 G L -1.1248 Soluble
122 T L 0.0000 Buried
123 K L -2.1710 Soluble
124 L L 0.0000 Buried
125 T L -0.7113 Soluble
126 V L -0.4673 Soluble
127 L L 1.2359 Aggregation-prone

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6353 3.8689 CSV PDB
4.5 -0.6649 3.872 CSV PDB
5.0 -0.6968 3.8806 CSV PDB
5.5 -0.7247 3.9009 CSV PDB
6.0 -0.7418 3.9342 CSV PDB
6.5 -0.7441 3.9697 CSV PDB
7.0 -0.7345 3.9936 CSV PDB
7.5 -0.7186 4.0046 CSV PDB
8.0 -0.6991 4.0085 CSV PDB
8.5 -0.6758 4.0092 CSV PDB
9.0 -0.6478 4.0076 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH