Bexatamig / 4jzj

A4DAB045_4jzj_H_L | Source PDB: 4jzj | Chains: H, L | Done
99.15
Yes
10 Å
Yes
Avg A4D Score
-0.7047
Max Score
2.0677
Min Score
-3.7695
Total Score
-305.83
Score CDR
-0.3218
Score non-CDR
-0.7579

Calculation Parameters

Chain Sequence(s) EVQLVQSGAEVKKPGESLKISCKGSGYSFTDYYMKWARQMPGKGLEWMGDIIPSNGATFYNQKFKGQVTISADKSISTTYLQWSSLKASDTAMYYCARSHLLRASWFAYWGQGTMVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIVMTQSPDSLAVSLGERATINCESSQSLLNSGNQKNYLTWYQQKPGQPPKPLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNDYSYPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7695
Maximum Score 2.0677
Average Score -0.7047
Total Score -305.8266

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1651 Soluble
2 V H -1.3127 Soluble
3 Q H -1.4088 Soluble
4 L H 0.0000 Buried
5 V H 0.7855 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.3315 Soluble
8 G H -0.4188 Soluble
9 A H -0.0674 Soluble
11 E H -0.2562 Soluble
12 V H -0.0012 Soluble
13 K H -1.2768 Soluble
14 K H -2.1238 Soluble
15 P H -2.0274 Soluble
16 G H -1.7805 Soluble
17 E H -1.7847 Soluble
18 S H -1.5003 Soluble
19 L H 0.0000 Buried
20 K H -1.6059 Soluble
21 I H 0.0000 Buried
22 S H -0.3412 Soluble
23 C H 0.0000 Buried
24 K H -0.5952 Soluble
25 G H 0.0000 Buried
26 S H -0.8235 Soluble
27 G H -1.2783 Soluble
28 Y H -0.8623 Soluble
29 S H -0.9429 Soluble
30 F H 0.0000 Buried
35 T H -1.3642 Soluble
36 D H -1.4133 Soluble
37 Y H -0.0463 Soluble
38 Y H 0.2560 Aggregation-prone
39 M H 0.0000 Buried
40 K H 0.0000 Buried
41 W H 0.0000 Buried
42 A H 0.0000 Buried
43 R H -0.5490 Soluble
44 Q H -0.7111 Soluble
45 M H -0.7507 Soluble
46 P H -0.8964 Soluble
47 G H -1.4777 Soluble
48 K H -2.2378 Soluble
49 G H -1.3210 Soluble
50 L H 0.0000 Buried
51 E H -0.8235 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 D H 0.0000 Buried
56 I H 0.0000 Buried
57 I H -0.3192 Soluble
58 P H 0.0000 Buried
59 S H -1.4715 Soluble
62 N H -1.7158 Soluble
63 G H -1.1785 Soluble
64 A H -0.1676 Soluble
65 T H 0.4442 Aggregation-prone
66 F H 0.9426 Aggregation-prone
67 Y H -0.4052 Soluble
68 N H 0.0000 Buried
69 Q H -2.6216 Soluble
70 K H -3.0845 Soluble
71 F H 0.0000 Buried
72 K H -3.0625 Soluble
74 G H -2.3824 Soluble
75 Q H -2.4934 Soluble
76 V H 0.0000 Buried
77 T H -1.0273 Soluble
78 I H 0.0000 Buried
79 S H -0.4994 Soluble
80 A H -1.0288 Soluble
81 D H -1.6666 Soluble
82 K H -2.2014 Soluble
83 S H -0.7539 Soluble
84 I H 0.0897 Aggregation-prone
85 S H -0.7597 Soluble
86 T H 0.0000 Buried
87 T H 0.0000 Buried
88 Y H -0.5122 Soluble
89 L H 0.0000 Buried
90 Q H -1.2922 Soluble
91 W H 0.0000 Buried
92 S H -1.0925 Soluble
93 S H -1.5518 Soluble
94 L H 0.0000 Buried
95 K H -2.4370 Soluble
96 A H -1.3726 Soluble
97 S H -0.9068 Soluble
98 D H -0.9015 Soluble
99 T H -0.4262 Soluble
100 A H 0.0000 Buried
101 M H 0.1148 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 S H 0.0000 Buried
108 H H 0.9833 Aggregation-prone
109 L H 1.9162 Aggregation-prone
110 L H 2.0677 Aggregation-prone
111 R H 0.7927 Aggregation-prone
112 A H 0.5504 Aggregation-prone
113 S H 0.0000 Buried
114 W H 0.0000 Buried
115 F H 0.0000 Buried
116 A H 0.2502 Aggregation-prone
117 Y H 0.0313 Aggregation-prone
118 W H -0.1722 Soluble
119 G H 0.0000 Buried
120 Q H -0.9663 Soluble
121 G H -0.4565 Soluble
122 T H 0.0000 Buried
123 M H 0.1527 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4192 Soluble
126 V H 0.0000 Buried
127 S H -0.9220 Soluble
128 S H -1.0523 Soluble
129 A H -0.6143 Soluble
130 S H -0.7058 Soluble
131 T H -0.7299 Soluble
132 K H -1.1549 Soluble
133 G H -1.3779 Soluble
134 P H 0.0000 Buried
135 S H -0.3424 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0909 Soluble
139 L H 0.0000 Buried
140 A H -1.1424 Soluble
141 P H 0.0000 Buried
142 S H -0.9942 Soluble
143 G H -0.6354 Soluble
144 T H -0.4814 Soluble
145 A H -0.2531 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.3453 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -1.1254 Soluble
158 P H -1.0343 Soluble
159 V H -0.7656 Soluble
160 T H -0.5678 Soluble
161 V H -0.1666 Soluble
162 S H -0.3968 Soluble
163 W H 0.0000 Buried
164 N H -0.7710 Soluble
165 S H -0.6816 Soluble
166 G H -0.5622 Soluble
167 A H -0.2364 Soluble
168 L H -0.0018 Soluble
169 T H -0.1784 Soluble
170 S H -0.1919 Soluble
171 G H -0.2319 Soluble
172 V H 0.1557 Aggregation-prone
173 H H -0.2841 Soluble
174 T H 0.0359 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.2673 Soluble
177 A H 0.1452 Aggregation-prone
178 V H 0.4366 Aggregation-prone
179 L H 0.9696 Aggregation-prone
180 Q H 0.1819 Aggregation-prone
181 S H -0.1024 Soluble
182 S H -0.2184 Soluble
183 G H -0.0199 Soluble
184 L H -0.0246 Soluble
185 Y H 0.2409 Aggregation-prone
186 S H 0.2251 Aggregation-prone
187 L H 0.1697 Aggregation-prone
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1470 Soluble
193 V H 0.0000 Buried
194 P H -0.5960 Soluble
195 S H -0.6315 Soluble
196 S H -0.6085 Soluble
197 S H -0.6205 Soluble
198 L H -0.8263 Soluble
199 G H -1.0293 Soluble
200 T H -0.7483 Soluble
201 Q H -1.2585 Soluble
202 T H -1.1604 Soluble
203 Y H 0.0000 Buried
204 I H -1.3605 Soluble
205 C H 0.0000 Buried
206 N H -1.4561 Soluble
207 V H 0.0000 Buried
208 N H -1.8323 Soluble
209 H H 0.0000 Buried
210 K H -2.8001 Soluble
211 P H -1.7259 Soluble
212 S H -1.8442 Soluble
213 N H -2.4803 Soluble
214 T H -2.2129 Soluble
215 K H -2.6876 Soluble
216 V H -1.6990 Soluble
217 D H -2.5243 Soluble
218 K H -2.2187 Soluble
219 K H -2.6219 Soluble
220 V H 0.0000 Buried
221 E H -2.8707 Soluble
222 P H -2.0628 Soluble
223 K H -2.5062 Soluble
1 D L -1.2704 Soluble
2 I L 0.0372 Aggregation-prone
3 V L 0.8594 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.8405 Soluble
6 Q L 0.0000 Buried
7 S L -1.3982 Soluble
8 P L -1.3057 Soluble
9 D L -2.1846 Soluble
10 S L -1.1316 Soluble
11 L L -0.5401 Soluble
12 A L -0.4320 Soluble
13 V L 0.0000 Buried
14 S L -1.3553 Soluble
15 L L -0.3375 Soluble
16 G L -1.2763 Soluble
17 E L -2.8787 Soluble
18 R L -2.8465 Soluble
19 A L 0.0000 Buried
20 T L -0.6768 Soluble
21 I L 0.0000 Buried
22 N L -1.7701 Soluble
23 C L 0.0000 Buried
24 E L -2.5291 Soluble
25 S L -1.3586 Soluble
26 S L -0.9523 Soluble
27 Q L -1.5633 Soluble
28 S L -1.1073 Soluble
29 L L 0.0000 Buried
30 L L -0.7967 Soluble
31 N L -1.2869 Soluble
32 S L -1.3761 Soluble
33 G L -1.4918 Soluble
34 N L -1.9653 Soluble
35 Q L -2.2257 Soluble
36 K L -1.7850 Soluble
37 N L -0.8575 Soluble
38 Y L -0.0931 Soluble
39 L L 0.0000 Buried
40 T L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -0.8727 Soluble
45 K L -1.2105 Soluble
46 P L -1.0379 Soluble
47 G L -1.2181 Soluble
48 Q L -1.6604 Soluble
49 P L -1.1892 Soluble
50 P L 0.0000 Buried
51 K L -1.4295 Soluble
52 P L -0.8262 Soluble
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0000 Buried
56 W L -0.3997 Soluble
57 A L 0.0000 Buried
65 S L -0.8202 Soluble
66 T L -0.8606 Soluble
67 R L -1.8256 Soluble
68 E L -1.4176 Soluble
69 S L -0.9817 Soluble
70 G L -1.1032 Soluble
71 V L 0.0000 Buried
72 P L -1.4300 Soluble
74 D L -2.2189 Soluble
75 R L -1.5368 Soluble
76 F L 0.0000 Buried
77 S L -0.9384 Soluble
78 G L 0.0000 Buried
79 S L -0.8458 Soluble
80 G L -1.2220 Soluble
83 S L -1.1756 Soluble
84 G L -1.3843 Soluble
85 T L -1.8983 Soluble
86 D L -2.9710 Soluble
87 F L 0.0000 Buried
88 T L -1.1484 Soluble
89 L L 0.0000 Buried
90 T L -0.6738 Soluble
91 I L 0.0000 Buried
92 S L -1.8469 Soluble
93 S L -1.7674 Soluble
94 L L 0.0000 Buried
95 Q L -1.1797 Soluble
96 A L -0.8979 Soluble
97 E L -1.7626 Soluble
98 D L 0.0000 Buried
99 V L -0.4441 Soluble
100 A L 0.0000 Buried
101 V L -0.2493 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 N L 0.0000 Buried
107 D L 0.0000 Buried
108 Y L 0.5537 Aggregation-prone
109 S L 0.2924 Aggregation-prone
114 Y L 0.6201 Aggregation-prone
115 P L 0.0000 Buried
116 Y L 0.3625 Aggregation-prone
117 T L 0.3510 Aggregation-prone
118 F L 0.2836 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.2220 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.9320 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.7291 Soluble
127 K L -1.5597 Soluble
128 R L -1.0267 Soluble
129 T L -0.2336 Soluble
130 V L 0.3254 Aggregation-prone
131 A L 0.0412 Aggregation-prone
132 A L -0.0991 Soluble
133 P L 0.0000 Buried
134 S L -0.1158 Soluble
135 V L 0.0000 Buried
136 F L 0.0568 Aggregation-prone
137 I L 0.0373 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.8399 Soluble
140 P L 0.0000 Buried
141 S L -1.4203 Soluble
142 D L -2.7154 Soluble
143 E L -2.4143 Soluble
144 Q L 0.0000 Buried
145 L L -2.0140 Soluble
146 K L -2.6346 Soluble
147 S L -1.6375 Soluble
148 G L -1.3368 Soluble
149 T L -0.9992 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7267 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3615 Soluble
162 R L -2.0710 Soluble
163 E L -2.7184 Soluble
164 A L -1.9550 Soluble
165 K L -2.0266 Soluble
166 V L -0.8468 Soluble
167 Q L -0.4937 Soluble
168 W L 0.0000 Buried
169 K L -0.6370 Soluble
170 V L 0.0000 Buried
171 D L -1.8692 Soluble
172 N L -1.5397 Soluble
173 A L -0.2654 Soluble
174 L L 0.6949 Aggregation-prone
175 Q L -0.1732 Soluble
176 S L -0.4370 Soluble
177 G L -0.8826 Soluble
178 N L -0.7636 Soluble
179 S L -1.0659 Soluble
180 Q L -1.3901 Soluble
181 E L -1.8829 Soluble
182 S L -0.9626 Soluble
183 V L -0.6908 Soluble
184 T L -1.0557 Soluble
185 E L -2.1459 Soluble
186 Q L -1.7300 Soluble
187 D L -1.9018 Soluble
188 S L -1.8482 Soluble
189 K L -2.2155 Soluble
190 D L -1.6766 Soluble
191 S L -1.7224 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6646 Soluble
199 L L 0.0000 Buried
200 T L -0.4379 Soluble
201 L L -0.7651 Soluble
202 S L -1.2239 Soluble
203 K L -2.2115 Soluble
204 A L -2.0925 Soluble
205 D L -3.1861 Soluble
206 Y L 0.0000 Buried
207 E L -3.7224 Soluble
208 K L -3.7695 Soluble
209 H L -3.1819 Soluble
210 K L -2.7672 Soluble
211 V L -1.4226 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8242 Soluble
216 V L 0.0000 Buried
217 T L -1.0269 Soluble
218 H L 0.0000 Buried
219 Q L -1.6472 Soluble
220 G L -0.4323 Soluble
221 L L -0.1806 Soluble
222 S L -0.4256 Soluble
223 S L -0.3627 Soluble
224 P L -0.4127 Soluble
225 V L 0.1138 Aggregation-prone
226 T L -0.4568 Soluble
227 K L -0.7223 Soluble
228 S L -0.6506 Soluble
229 F L -1.0982 Soluble
230 N L -2.1458 Soluble
231 R L -2.4617 Soluble
232 G L -2.2902 Soluble
233 E L -2.7055 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7025 2.5172 CSV PDB
4.5 -0.7525 2.4963 CSV PDB
5.0 -0.8107 2.4577 CSV PDB
5.5 -0.8648 2.4038 CSV PDB
6.0 -0.9013 2.3411 CSV PDB
6.5 -0.9101 2.319 CSV PDB
7.0 -0.8919 2.3194 CSV PDB
7.5 -0.8566 2.3661 CSV PDB
8.0 -0.8123 2.4326 CSV PDB
8.5 -0.7613 2.4997 CSV PDB
9.0 -0.7033 2.5665 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH