Ansuvimab / 5fhc

A4DAB020_5fhc_H_L | Source PDB: 5fhc | Chains: H, L | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.7154
Max Score
1.1496
Min Score
-3.616
Total Score
-304.75
Score CDR
-0.6296
Score non-CDR
-0.7258

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLIQPGGSLRLSCAASGFALRMYDMHWVRQTIDKRLEWVSAVGPSGDTYYADSVKGRFAVSRENAKNSLSLQMNSLTAGDTAIYYCVRSDRGVAGLFDSWGQGILVTVSSASTKGPSVFPLAPSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKIQMTQSPSSLSASVGDRITITCRASQAFDNYVAWYQQRPGKVPKLLISAASALHAGVPSRFSGSGSGTHFTLTISSLQPEDVATYYCQNYNSAPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.616
Maximum Score 1.1496
Average Score -0.7154
Total Score -304.7473

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1688 Soluble
2 V H -1.3857 Soluble
3 Q H -1.4054 Soluble
4 L H 0.0000 Buried
5 V H 1.1496 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.2032 Soluble
8 G H -1.0134 Soluble
9 G H -0.4378 Soluble
11 G H -0.3735 Soluble
12 L H -0.0321 Soluble
13 I H -0.4287 Soluble
14 Q H -1.3860 Soluble
15 P H -1.1572 Soluble
16 G H -1.1157 Soluble
17 G H -0.8771 Soluble
18 S H -1.1222 Soluble
19 L H -1.1976 Soluble
20 R H -2.2412 Soluble
21 L H 0.0000 Buried
22 S H -0.5547 Soluble
23 C H 0.0000 Buried
24 A H 0.0579 Aggregation-prone
25 A H 0.0000 Buried
26 S H -0.8353 Soluble
27 G H -1.3468 Soluble
28 F H -0.6262 Soluble
29 A H -0.7390 Soluble
30 L H 0.0000 Buried
35 R H -2.0940 Soluble
36 M H -1.0789 Soluble
37 Y H 0.0000 Buried
38 D H -1.2300 Soluble
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H 0.0000 Buried
45 T H -0.3245 Soluble
46 I H 0.4722 Aggregation-prone
47 D H -1.9162 Soluble
48 K H -2.2633 Soluble
49 R H -1.9156 Soluble
50 L H 0.0000 Buried
51 E H -0.6198 Soluble
52 W H 0.0297 Aggregation-prone
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 A H 0.0000 Buried
56 V H 0.0000 Buried
57 G H 0.0000 Buried
58 P H -1.4683 Soluble
59 S H -1.2350 Soluble
63 G H -1.2635 Soluble
64 D H -0.9089 Soluble
65 T H 0.0060 Aggregation-prone
66 Y H 0.6788 Aggregation-prone
67 Y H -0.3520 Soluble
68 A H -1.1050 Soluble
69 D H -2.4634 Soluble
70 S H -1.6933 Soluble
71 V H 0.0000 Buried
72 K H -2.5295 Soluble
74 G H -1.8331 Soluble
75 R H -1.6882 Soluble
76 F H 0.0000 Buried
77 A H -0.7355 Soluble
78 V H 0.0000 Buried
79 S H -0.9412 Soluble
80 R H -1.7593 Soluble
81 E H -2.5092 Soluble
82 N H -2.3913 Soluble
83 A H -1.7969 Soluble
84 K H -2.3763 Soluble
85 N H -1.9320 Soluble
86 S H -1.1863 Soluble
87 L H 0.0000 Buried
88 S H -0.9997 Soluble
89 L H 0.0000 Buried
90 Q H -1.5013 Soluble
91 M H 0.0000 Buried
92 N H -1.1963 Soluble
93 S H -0.9684 Soluble
94 L H 0.0000 Buried
95 T H -0.5923 Soluble
96 A H -0.4228 Soluble
97 G H -0.1201 Soluble
98 D H 0.0000 Buried
99 T H 0.5115 Aggregation-prone
100 A H 0.0000 Buried
101 I H 0.6969 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 V H 0.0000 Buried
106 R H -0.8094 Soluble
107 S H 0.0000 Buried
108 D H -1.5235 Soluble
109 R H -2.1452 Soluble
110 G H -1.1911 Soluble
111 V H -0.4507 Soluble
112 A H -0.4596 Soluble
113 G H 0.0000 Buried
114 L H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -1.3199 Soluble
117 S H -0.8173 Soluble
118 W H -0.5512 Soluble
119 G H 0.0000 Buried
120 Q H -1.0059 Soluble
121 G H -0.1890 Soluble
122 I H 0.3305 Aggregation-prone
123 L H 0.4502 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.5590 Soluble
128 S H -0.5958 Soluble
129 A H -0.3901 Soluble
130 S H -0.5136 Soluble
131 T H -0.8177 Soluble
132 K H -1.2028 Soluble
133 G H -1.3425 Soluble
134 P H 0.0000 Buried
135 S H -0.4236 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1127 Soluble
139 L H 0.0000 Buried
140 A H -1.2047 Soluble
141 P H 0.0000 Buried
142 S H -0.6874 Soluble
143 G H -0.8243 Soluble
144 G H -0.8593 Soluble
145 T H -0.6408 Soluble
146 A H 0.0000 Buried
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.3054 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.4399 Soluble
159 P H -0.5544 Soluble
160 V H -0.6678 Soluble
161 T H -0.6051 Soluble
162 V H -0.2704 Soluble
163 S H -0.4068 Soluble
164 W H 0.0000 Buried
165 N H -0.7085 Soluble
166 S H -0.6433 Soluble
167 G H -0.5058 Soluble
168 A H -0.2309 Soluble
169 L H -0.0086 Soluble
170 T H -0.1846 Soluble
171 S H -0.2054 Soluble
172 G H -0.2623 Soluble
173 V H 0.1160 Aggregation-prone
174 H H -0.2505 Soluble
175 T H -0.1253 Soluble
176 F H 0.0000 Buried
177 P H -0.3661 Soluble
178 A H 0.2683 Aggregation-prone
179 V H 0.6110 Aggregation-prone
180 L H 1.1222 Aggregation-prone
181 Q H 0.2721 Aggregation-prone
182 S H -0.0945 Soluble
183 S H -0.1937 Soluble
184 G H 0.0740 Aggregation-prone
185 L H 0.2235 Aggregation-prone
186 Y H 0.4792 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1548 Soluble
194 V H 0.0000 Buried
195 P H -0.6251 Soluble
196 S H -0.5918 Soluble
197 S H -0.6275 Soluble
198 S H -0.5831 Soluble
199 L H -0.9280 Soluble
200 G H -1.2255 Soluble
201 T H -0.8691 Soluble
202 Q H -1.1591 Soluble
203 T H -1.0107 Soluble
204 Y H 0.0000 Buried
205 I H -1.0732 Soluble
206 C H 0.0000 Buried
207 N H -1.5063 Soluble
208 V H 0.0000 Buried
209 N H -2.1225 Soluble
210 H H 0.0000 Buried
211 K H -2.8325 Soluble
212 P H -1.5917 Soluble
213 S H -1.8192 Soluble
214 N H -2.6097 Soluble
215 T H -2.1269 Soluble
216 K H -2.8140 Soluble
217 V H -1.7075 Soluble
218 D H -2.5433 Soluble
219 K H -2.1014 Soluble
220 K H -2.1671 Soluble
221 V H 0.0000 Buried
222 E H -2.7070 Soluble
223 P H -1.9876 Soluble
224 K H -2.2436 Soluble
2 I L 0.3233 Aggregation-prone
3 Q L -1.1771 Soluble
4 M L 0.0000 Buried
5 T L -1.2874 Soluble
6 Q L -1.0092 Soluble
7 S L -1.0028 Soluble
8 P L -0.7496 Soluble
9 S L -0.9731 Soluble
10 S L -1.1132 Soluble
11 L L -0.8457 Soluble
12 S L -1.2674 Soluble
13 A L -1.5308 Soluble
14 S L -1.4737 Soluble
15 V L -0.4588 Soluble
16 G L -0.8705 Soluble
17 D L -2.0058 Soluble
18 R L -2.3788 Soluble
19 I L 0.0000 Buried
20 T L -0.4945 Soluble
21 I L 0.0000 Buried
22 T L -0.9086 Soluble
23 C L 0.0000 Buried
24 R L -2.7222 Soluble
25 A L 0.0000 Buried
26 S L -1.3990 Soluble
27 Q L -1.3631 Soluble
28 A L -1.2793 Soluble
29 F L -0.9016 Soluble
36 D L -1.8724 Soluble
37 N L -1.3701 Soluble
38 Y L -0.3470 Soluble
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.5476 Soluble
45 R L -2.1940 Soluble
46 P L -1.7607 Soluble
47 G L -1.7122 Soluble
48 K L -2.4851 Soluble
49 V L -1.4986 Soluble
50 P L 0.0000 Buried
51 K L -1.9877 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 S L 0.0000 Buried
56 A L -0.2016 Soluble
57 A L 0.0000 Buried
65 S L -0.2188 Soluble
66 A L 0.1341 Aggregation-prone
67 L L 0.3888 Aggregation-prone
68 H L -0.2651 Soluble
69 A L -0.1706 Soluble
70 G L -0.3572 Soluble
71 V L -0.0862 Soluble
72 P L -0.2743 Soluble
74 S L -0.3843 Soluble
75 R L -0.7798 Soluble
76 F L 0.0000 Buried
77 S L -0.3061 Soluble
78 G L -0.3201 Soluble
79 S L -0.7127 Soluble
80 G L -1.1533 Soluble
83 S L -1.1209 Soluble
84 G L -1.4655 Soluble
85 T L -1.8364 Soluble
86 H L -1.9628 Soluble
87 F L 0.0000 Buried
88 T L -0.7941 Soluble
89 L L 0.0000 Buried
90 T L -0.6130 Soluble
91 I L 0.0000 Buried
92 S L -1.4040 Soluble
93 S L -1.1539 Soluble
94 L L 0.0000 Buried
95 Q L -0.8321 Soluble
96 P L -1.2915 Soluble
97 E L -1.7971 Soluble
98 D L 0.0000 Buried
99 V L -1.1298 Soluble
100 A L 0.0000 Buried
101 T L -1.3060 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 N L 0.0000 Buried
107 Y L 0.0000 Buried
108 N L -0.5304 Soluble
109 S L -0.2173 Soluble
114 A L 0.0736 Aggregation-prone
115 P L -0.1247 Soluble
116 L L 0.0940 Aggregation-prone
117 T L 0.0000 Buried
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.5233 Soluble
121 G L -1.2675 Soluble
122 T L 0.0000 Buried
123 K L -2.0876 Soluble
124 V L 0.0000 Buried
125 E L -1.4250 Soluble
126 I L 0.0000 Buried
127 K L -2.4673 Soluble
128 R L -2.1931 Soluble
129 T L -1.0666 Soluble
130 V L -0.5723 Soluble
131 A L -0.2386 Soluble
132 A L -0.1760 Soluble
133 P L 0.0000 Buried
134 S L -0.1634 Soluble
135 V L 0.0000 Buried
136 F L 0.1526 Aggregation-prone
137 I L 0.1428 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.7840 Soluble
140 P L -0.8810 Soluble
141 S L -1.8504 Soluble
142 D L -3.0205 Soluble
143 E L -3.1145 Soluble
144 Q L 0.0000 Buried
145 L L -2.3822 Soluble
146 K L -2.9662 Soluble
147 S L -1.8593 Soluble
148 G L -1.3541 Soluble
149 T L -1.1199 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8165 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L 0.0000 Buried
162 R L -2.5588 Soluble
163 E L -2.9597 Soluble
164 A L -2.0806 Soluble
165 K L -1.9512 Soluble
166 V L -1.0771 Soluble
167 Q L -0.5640 Soluble
168 W L 0.0000 Buried
169 K L -0.5247 Soluble
170 V L 0.0000 Buried
171 D L -1.8423 Soluble
172 N L -1.4930 Soluble
173 A L -0.2294 Soluble
174 L L 0.6677 Aggregation-prone
175 Q L -0.2715 Soluble
176 S L -0.4264 Soluble
177 G L -0.9032 Soluble
178 N L -0.8237 Soluble
179 S L -1.0075 Soluble
180 Q L -1.1449 Soluble
181 E L -1.4180 Soluble
182 S L -0.7122 Soluble
183 V L -0.5722 Soluble
184 T L -0.8221 Soluble
185 E L -1.5230 Soluble
186 Q L 0.0000 Buried
187 D L -1.7888 Soluble
188 S L -2.0157 Soluble
189 K L -2.4207 Soluble
190 D L -1.7038 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L -1.0976 Soluble
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5400 Soluble
199 L L 0.0000 Buried
200 T L -0.4424 Soluble
201 L L -0.7042 Soluble
202 S L -1.2852 Soluble
203 K L -2.1546 Soluble
204 A L -1.9926 Soluble
205 D L -2.6349 Soluble
206 Y L 0.0000 Buried
207 E L -3.6159 Soluble
208 K L -3.6160 Soluble
209 H L -3.1086 Soluble
210 K L -3.3466 Soluble
211 V L -1.7631 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6402 Soluble
216 V L 0.0000 Buried
217 T L -1.0333 Soluble
218 H L 0.0000 Buried
219 Q L -1.5672 Soluble
220 G L -0.5540 Soluble
221 L L -0.2919 Soluble
222 S L -0.4763 Soluble
223 S L -0.4067 Soluble
224 P L -0.5173 Soluble
225 V L -0.0539 Soluble
226 T L -0.4533 Soluble
227 K L -0.6178 Soluble
228 S L -0.7310 Soluble
229 F L -0.9878 Soluble
230 N L -2.1993 Soluble
231 R L -2.5157 Soluble
232 G L -1.5118 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8074 1.9187 CSV PDB
4.5 -0.8486 1.8711 CSV PDB
5.0 -0.8945 1.8214 CSV PDB
5.5 -0.9359 1.7709 CSV PDB
6.0 -0.9625 1.7203 CSV PDB
6.5 -0.9667 1.6699 CSV PDB
7.0 -0.9497 1.6272 CSV PDB
7.5 -0.919 1.6272 CSV PDB
8.0 -0.8801 1.6272 CSV PDB
8.5 -0.8348 1.6272 CSV PDB
9.0 -0.7829 1.6272 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH