Tislelizumab / 7cgw

A4DAB269_7cgw_H_L | Source PDB: 7cgw | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6842
Max Score
1.2712
Min Score
-3.8175
Total Score
-294.9
Score CDR
-0.1218
Score non-CDR
-0.7498

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSETLSLTCTVSGFSLTSYGVHWIRQPPGKGLEWIGVIYADGSTNYNPSLKSRVTISKDTSKNQVSLKLSSVTAADTAVYYCARAYGNYWYIDVWGQGTTVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESIVMTQSPDSLAVSLGERATINCKSSESVSNDVAWYQQKPGQPPKLLINYAFHRFTGVPDRFSGSGYGTDFTLTISSLQAEDVAVYYCHQAYSSPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8175
Maximum Score 1.2712
Average Score -0.6842
Total Score -294.8984

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.2409 Soluble
2 V H -0.4357 Soluble
3 Q H -1.0527 Soluble
4 L H 0.0000 Buried
5 Q H -1.6247 Soluble
6 E H -1.1192 Soluble
7 S H -0.6586 Soluble
8 G H -0.3148 Soluble
9 P H -0.3761 Soluble
11 G H 0.0000 Buried
12 L H -0.2208 Soluble
13 V H 0.0000 Buried
14 K H -1.6727 Soluble
15 P H -1.2502 Soluble
16 S H -1.2910 Soluble
17 E H -1.8188 Soluble
18 T H -1.2936 Soluble
19 L H 0.0000 Buried
20 S H -0.7641 Soluble
21 L H 0.0000 Buried
22 T H -0.6755 Soluble
23 C H 0.0000 Buried
24 T H -1.2316 Soluble
25 V H 0.0000 Buried
26 S H -0.8600 Soluble
27 G H -0.7579 Soluble
28 F H -0.2511 Soluble
29 S H -0.4645 Soluble
30 L H 0.0000 Buried
35 T H -0.6795 Soluble
36 S H -0.3219 Soluble
37 Y H -0.0451 Soluble
38 G H -0.0470 Soluble
39 V H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 I H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.9321 Soluble
45 P H -1.0229 Soluble
46 P H -1.0545 Soluble
47 G H -1.5083 Soluble
48 K H -2.4364 Soluble
49 G H -1.7413 Soluble
50 L H 0.0000 Buried
51 E H -1.4476 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.4433 Soluble
58 A H -0.8189 Soluble
59 D H -1.8553 Soluble
63 G H -1.2522 Soluble
64 S H -0.7598 Soluble
65 T H -0.4076 Soluble
66 N H -0.2008 Soluble
67 Y H -0.2214 Soluble
68 N H 0.0000 Buried
69 P H -0.9488 Soluble
70 S H -0.9767 Soluble
71 L H 0.0000 Buried
72 K H -2.0007 Soluble
74 S H -1.2420 Soluble
75 R H -1.2230 Soluble
76 V H 0.0000 Buried
77 T H -0.5953 Soluble
78 I H 0.0000 Buried
79 S H -0.8209 Soluble
80 K H -1.2728 Soluble
81 D H -1.8228 Soluble
82 T H -1.4198 Soluble
83 S H -1.5161 Soluble
84 K H -2.3621 Soluble
85 N H -1.6984 Soluble
86 Q H -1.6788 Soluble
87 V H 0.0000 Buried
88 S H -0.7584 Soluble
89 L H 0.0000 Buried
90 K H -1.2644 Soluble
91 L H 0.0000 Buried
92 S H -1.1730 Soluble
93 S H -0.9945 Soluble
94 V H 0.0000 Buried
95 T H -0.5579 Soluble
96 A H -0.1616 Soluble
97 A H 0.0480 Aggregation-prone
98 D H 0.0000 Buried
99 T H 0.0287 Aggregation-prone
100 A H 0.0000 Buried
101 V H 0.0984 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 A H 0.0000 Buried
108 Y H 0.5679 Aggregation-prone
109 G H -0.1854 Soluble
110 N H -0.5595 Soluble
112 Y H 0.9354 Aggregation-prone
113 W H 0.6059 Aggregation-prone
114 Y H 0.0000 Buried
115 I H 0.0000 Buried
116 D H 0.0329 Aggregation-prone
117 V H 0.0420 Aggregation-prone
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.6332 Soluble
121 G H -0.9794 Soluble
122 T H -0.5118 Soluble
123 T H -0.1855 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.5561 Soluble
128 S H -0.6909 Soluble
129 A H -0.5581 Soluble
130 S H -0.7247 Soluble
131 T H -0.8981 Soluble
132 K H -1.6972 Soluble
133 G H -1.6909 Soluble
134 P H -0.8074 Soluble
135 S H -0.3821 Soluble
136 V H -0.2429 Soluble
137 F H -0.4721 Soluble
138 P H -1.0232 Soluble
139 L H 0.0000 Buried
140 A H -0.7148 Soluble
141 P H -0.6601 Soluble
142 C H -0.8176 Soluble
143 S H -1.3152 Soluble
144 R H -2.2174 Soluble
145 S H -1.7729 Soluble
146 T H -1.4695 Soluble
147 S H -1.4866 Soluble
148 E H -2.3818 Soluble
149 S H -1.3271 Soluble
150 T H -0.9033 Soluble
151 A H -0.8637 Soluble
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.7904 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.5338 Soluble
164 P H -0.6271 Soluble
165 V H -0.4499 Soluble
166 T H -0.6038 Soluble
167 V H -0.2987 Soluble
168 S H -0.5149 Soluble
169 W H 0.0000 Buried
170 N H -0.9784 Soluble
171 S H -0.7898 Soluble
172 G H -0.5864 Soluble
173 A H -0.2516 Soluble
174 L H -0.0736 Soluble
175 T H -0.1964 Soluble
176 S H -0.2414 Soluble
177 G H -0.3108 Soluble
178 V H 0.1104 Aggregation-prone
179 H H -0.2572 Soluble
180 T H 0.0335 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3892 Soluble
183 A H 0.4216 Aggregation-prone
184 V H 0.5147 Aggregation-prone
185 L H 0.9569 Aggregation-prone
186 Q H 0.0079 Aggregation-prone
187 S H -0.2602 Soluble
188 S H -0.4040 Soluble
189 G H -0.2440 Soluble
190 L H -0.2041 Soluble
191 Y H 0.1733 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1355 Soluble
199 V H 0.0000 Buried
200 P H -0.5891 Soluble
201 S H -1.0147 Soluble
202 S H -0.6278 Soluble
203 S H -0.5967 Soluble
204 L H -1.0918 Soluble
205 G H -1.1107 Soluble
206 T H -0.8721 Soluble
207 K H -1.5358 Soluble
208 T H -1.4581 Soluble
209 Y H 0.0000 Buried
210 T H -1.9440 Soluble
211 C H 0.0000 Buried
212 N H -1.7154 Soluble
213 V H 0.0000 Buried
214 D H -2.0405 Soluble
215 H H 0.0000 Buried
216 K H -2.5699 Soluble
217 P H -1.5983 Soluble
218 S H -1.9247 Soluble
219 N H -2.3808 Soluble
220 T H -2.1474 Soluble
221 K H -2.7628 Soluble
222 V H -1.8595 Soluble
223 D H -2.7379 Soluble
224 K H -2.3662 Soluble
225 R H -2.9190 Soluble
226 V H 0.0000 Buried
227 E H -2.6151 Soluble
228 S H -1.6409 Soluble
2 I L 0.8977 Aggregation-prone
3 V L 1.2712 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.8081 Soluble
6 Q L 0.0000 Buried
7 S L -1.3682 Soluble
8 P L -1.3213 Soluble
9 D L -2.3724 Soluble
10 S L -1.3886 Soluble
11 L L -0.6761 Soluble
12 A L -0.4419 Soluble
13 V L -1.0688 Soluble
14 S L -1.3505 Soluble
15 L L -0.9498 Soluble
16 G L -1.7170 Soluble
17 E L -2.9061 Soluble
18 R L -2.9454 Soluble
19 A L 0.0000 Buried
20 T L -0.6156 Soluble
21 I L 0.0000 Buried
22 N L -1.4081 Soluble
23 C L 0.0000 Buried
24 K L -1.8924 Soluble
25 S L -0.9414 Soluble
26 S L -0.8896 Soluble
27 E L -1.6634 Soluble
28 S L -1.0916 Soluble
29 V L 0.0000 Buried
36 S L 0.0959 Aggregation-prone
37 N L 0.2682 Aggregation-prone
38 D L 0.6503 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.3852 Soluble
46 P L -1.2064 Soluble
47 G L -1.1242 Soluble
48 Q L -1.4241 Soluble
49 P L -1.1151 Soluble
50 P L 0.0000 Buried
51 K L -1.3146 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 N L 0.0000 Buried
56 Y L 0.5343 Aggregation-prone
57 A L 0.0000 Buried
65 F L 0.8121 Aggregation-prone
66 H L -0.6361 Soluble
67 R L -1.2295 Soluble
68 F L -0.2578 Soluble
69 T L -0.4202 Soluble
70 G L -0.7158 Soluble
71 V L -0.8133 Soluble
72 P L -1.2376 Soluble
74 D L -2.2313 Soluble
75 R L -1.7170 Soluble
76 F L 0.0000 Buried
77 S L -0.6853 Soluble
78 G L -0.0707 Soluble
79 S L 0.1339 Aggregation-prone
80 G L 0.1111 Aggregation-prone
83 Y L 0.7964 Aggregation-prone
84 G L -0.4398 Soluble
85 T L -1.2744 Soluble
86 D L -1.5803 Soluble
87 F L 0.0000 Buried
88 T L -0.4885 Soluble
89 L L 0.0000 Buried
90 T L -0.6795 Soluble
91 I L 0.0000 Buried
92 S L -1.9613 Soluble
93 S L -1.9540 Soluble
94 L L 0.0000 Buried
95 Q L -1.9144 Soluble
96 A L -1.9180 Soluble
97 E L -2.3489 Soluble
98 D L 0.0000 Buried
99 V L 0.0000 Buried
100 A L 0.0000 Buried
101 V L -0.7057 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 H L 0.0000 Buried
106 Q L 0.0000 Buried
107 A L 0.0000 Buried
108 Y L 0.8976 Aggregation-prone
109 S L 0.2021 Aggregation-prone
114 S L 0.1098 Aggregation-prone
115 P L 0.1512 Aggregation-prone
116 Y L 0.0000 Buried
117 T L 0.2181 Aggregation-prone
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.8321 Soluble
121 G L -1.4959 Soluble
122 T L 0.0000 Buried
123 K L -1.4398 Soluble
124 L L 0.0000 Buried
125 E L -1.2856 Soluble
126 I L 0.0000 Buried
127 K L -1.1059 Soluble
128 R L -0.6727 Soluble
129 T L 0.2635 Aggregation-prone
130 V L 1.0768 Aggregation-prone
131 A L 0.3404 Aggregation-prone
132 A L 0.1328 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.1758 Soluble
135 V L 0.0846 Aggregation-prone
136 F L 0.1492 Aggregation-prone
137 I L 0.0601 Aggregation-prone
138 F L 0.0000 Buried
139 P L 0.0000 Buried
140 P L 0.0000 Buried
141 S L -1.2091 Soluble
142 D L -2.4639 Soluble
143 E L -1.9721 Soluble
144 Q L 0.0000 Buried
145 L L -1.7344 Soluble
146 K L -2.4130 Soluble
147 S L -1.4909 Soluble
148 G L -1.0088 Soluble
149 T L -0.8387 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7193 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.4818 Soluble
162 R L -2.8934 Soluble
163 E L -3.2023 Soluble
164 A L -2.4238 Soluble
165 K L -2.5688 Soluble
166 V L 0.0000 Buried
167 Q L -0.7703 Soluble
168 W L 0.0000 Buried
169 K L -0.5938 Soluble
170 V L 0.0000 Buried
171 D L -1.4396 Soluble
172 N L -1.1958 Soluble
173 A L -0.2344 Soluble
174 L L 0.6686 Aggregation-prone
175 Q L -0.4042 Soluble
176 S L -0.6230 Soluble
177 G L -1.2121 Soluble
178 N L -1.7511 Soluble
179 S L -1.6256 Soluble
180 Q L -1.6172 Soluble
181 E L -2.0008 Soluble
182 S L -1.0619 Soluble
183 V L -0.9298 Soluble
184 T L -1.1768 Soluble
185 E L -2.2634 Soluble
186 Q L 0.0000 Buried
187 D L -1.8543 Soluble
188 S L -2.2696 Soluble
189 K L -2.5186 Soluble
190 D L -1.7411 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8013 Soluble
199 L L 0.0000 Buried
200 T L -0.7673 Soluble
201 L L -0.9987 Soluble
202 S L -1.3478 Soluble
203 K L -2.1886 Soluble
204 A L -2.1619 Soluble
205 D L -2.8307 Soluble
206 Y L 0.0000 Buried
207 E L -3.8175 Soluble
208 K L -3.7168 Soluble
209 H L -3.0148 Soluble
210 K L -3.2742 Soluble
211 V L -1.5370 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9429 Soluble
216 V L 0.0000 Buried
217 T L -1.2420 Soluble
218 H L 0.0000 Buried
219 Q L -1.6207 Soluble
220 G L -0.1796 Soluble
221 L L -0.1152 Soluble
222 S L -0.4263 Soluble
223 S L -0.4191 Soluble
224 P L -0.5938 Soluble
225 V L -0.0580 Soluble
226 T L -0.5572 Soluble
227 K L -0.8564 Soluble
228 S L -0.8321 Soluble
229 F L 0.0000 Buried
230 N L -2.3577 Soluble
231 R L -2.7902 Soluble
232 G L -2.2934 Soluble
233 E L -2.3628 Soluble
234 C L -0.8737 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7311 1.4843 CSV PDB
4.5 -0.7776 1.4745 CSV PDB
5.0 -0.8337 1.4637 CSV PDB
5.5 -0.8882 1.4527 CSV PDB
6.0 -0.9286 1.4416 CSV PDB
6.5 -0.9447 1.4305 CSV PDB
7.0 -0.9358 1.4195 CSV PDB
7.5 -0.9104 1.4086 CSV PDB
8.0 -0.876 1.4198 CSV PDB
8.5 -0.8352 1.5317 CSV PDB
9.0 -0.7882 1.6968 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH