Tislelizumab / 7bxa

A4DAB269_7bxa_H_L | Source PDB: 7bxa | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7091
Max Score
1.4831
Min Score
-4.052
Total Score
-299.26
Score CDR
-0.146
Score non-CDR
-0.7764

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSETLSLTCTVSGFSLTSYGVHWIRQPPGKGLEWIGVIYADGSTNYNPSLKSRVTISKDTSKNQVSLKLSSVTAADTAVYYCARAYGNYWYIDVWGQGTTVTVSSASTKGPSVFPLAPCAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKDIVMTQSPDSLAVSLGERATINCKSSESVSNDVAWYQQKPGQPPKLLINYAFHRFTGVPDRFSGSGYGTDFTLTISSLQAEDVAVYYCHQAYSSPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -4.052
Maximum Score 1.4831
Average Score -0.7091
Total Score -299.2578

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.5209 Soluble
2 V H -0.9801 Soluble
3 Q H -1.8435 Soluble
4 L H 0.0000 Buried
5 Q H -1.8584 Soluble
6 E H 0.0000 Buried
7 S H -0.8464 Soluble
8 G H -0.8036 Soluble
9 P H -0.5977 Soluble
11 G H 0.0000 Buried
12 L H -0.3129 Soluble
13 V H 0.0000 Buried
14 K H -2.4006 Soluble
15 P H -1.6987 Soluble
16 S H -1.7007 Soluble
17 E H -2.5881 Soluble
18 T H -1.7340 Soluble
19 L H 0.0000 Buried
20 S H -0.9272 Soluble
21 L H 0.0000 Buried
22 T H -0.7173 Soluble
23 C H 0.0000 Buried
24 T H -1.4138 Soluble
25 V H 0.0000 Buried
26 S H -1.4368 Soluble
27 G H -1.2349 Soluble
28 F H 0.0000 Buried
29 S H -0.3998 Soluble
30 L H 0.0000 Buried
35 T H -0.5429 Soluble
36 S H -0.3151 Soluble
37 Y H -0.0237 Soluble
38 G H 0.0000 Buried
39 V H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 I H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -1.1314 Soluble
45 P H -1.0688 Soluble
46 P H -0.9988 Soluble
47 G H -1.4955 Soluble
48 K H -2.4346 Soluble
49 G H -1.7138 Soluble
50 L H 0.0000 Buried
51 E H -2.1435 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.4438 Soluble
58 A H -0.9019 Soluble
59 D H -1.9085 Soluble
63 G H -1.3213 Soluble
64 S H -0.8174 Soluble
65 T H -0.3426 Soluble
66 N H -0.7210 Soluble
67 Y H -0.8486 Soluble
68 N H -1.3160 Soluble
69 P H -1.2685 Soluble
70 S H -0.8059 Soluble
71 L H -1.0565 Soluble
72 K H -2.1020 Soluble
74 S H -1.3382 Soluble
75 R H -1.5285 Soluble
76 V H 0.0000 Buried
77 T H -0.9389 Soluble
78 I H 0.0000 Buried
79 S H -0.8142 Soluble
80 K H -1.2826 Soluble
81 D H -1.6456 Soluble
82 T H -1.3953 Soluble
83 S H -1.4949 Soluble
84 K H -2.4222 Soluble
85 N H -1.9303 Soluble
86 Q H -1.7090 Soluble
87 V H 0.0000 Buried
88 S H -0.6982 Soluble
89 L H 0.0000 Buried
90 K H -1.3721 Soluble
91 L H 0.0000 Buried
92 S H -1.3168 Soluble
93 S H -1.3432 Soluble
94 V H 0.0000 Buried
95 T H -0.6877 Soluble
96 A H -0.1904 Soluble
97 A H 0.0655 Aggregation-prone
98 D H 0.0000 Buried
99 T H 0.0436 Aggregation-prone
100 A H 0.0000 Buried
101 V H 0.0483 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 A H 0.0000 Buried
108 Y H 0.6867 Aggregation-prone
109 G H -0.1773 Soluble
110 N H -0.5935 Soluble
112 Y H 0.8280 Aggregation-prone
113 W H 0.5901 Aggregation-prone
114 Y H 0.0000 Buried
115 I H 0.0000 Buried
116 D H 0.0000 Buried
117 V H -0.0517 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.6909 Soluble
121 G H -1.0014 Soluble
122 T H -0.7062 Soluble
123 T H -0.2062 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.7441 Soluble
128 S H -0.8216 Soluble
129 A H -0.5465 Soluble
130 S H -0.6431 Soluble
131 T H -0.5657 Soluble
132 K H -1.0109 Soluble
133 G H -1.2950 Soluble
134 P H 0.0000 Buried
135 S H -0.4285 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1713 Soluble
139 L H 0.0000 Buried
140 A H -0.9696 Soluble
141 P H 0.0000 Buried
142 C H 0.1958 Aggregation-prone
143 A H 0.0993 Aggregation-prone
144 A H 0.0000 Buried
145 L H 0.0000 Buried
146 G H 0.0000 Buried
147 C H 0.0000 Buried
148 L H 0.0000 Buried
149 V H 0.0000 Buried
150 K H 0.0000 Buried
151 D H -0.3298 Soluble
152 Y H 0.0000 Buried
153 F H 0.0000 Buried
154 P H 0.0000 Buried
155 E H -0.4133 Soluble
156 P H -0.7460 Soluble
157 V H 0.0000 Buried
158 T H -0.6434 Soluble
159 V H -0.4441 Soluble
160 S H -0.4757 Soluble
161 W H 0.0000 Buried
162 N H -1.2041 Soluble
163 S H -0.8426 Soluble
164 G H -0.5828 Soluble
165 A H -0.2624 Soluble
166 L H -0.0429 Soluble
167 T H -0.1446 Soluble
168 S H -0.3674 Soluble
169 G H -0.4136 Soluble
170 V H 0.2360 Aggregation-prone
171 H H 0.0000 Buried
172 T H -0.1327 Soluble
173 F H 0.0000 Buried
174 P H -0.4778 Soluble
175 A H 0.1350 Aggregation-prone
176 V H 0.4328 Aggregation-prone
177 L H 1.1339 Aggregation-prone
178 Q H 0.2447 Aggregation-prone
179 S H -0.0804 Soluble
180 S H -0.2082 Soluble
181 G H 0.0578 Aggregation-prone
182 L H 0.1721 Aggregation-prone
183 Y H 0.4833 Aggregation-prone
184 S H 0.0000 Buried
185 L H 0.0000 Buried
186 S H 0.0000 Buried
187 S H 0.0000 Buried
188 V H 0.0000 Buried
189 V H 0.0000 Buried
190 T H -0.0628 Soluble
191 V H 0.0339 Aggregation-prone
192 P H -0.2937 Soluble
193 S H -0.3577 Soluble
194 S H -0.5753 Soluble
195 S H -0.7181 Soluble
196 L H -0.8297 Soluble
197 G H -0.8836 Soluble
198 T H -1.0677 Soluble
199 K H -2.0251 Soluble
200 T H -1.7362 Soluble
201 Y H 0.0000 Buried
202 T H -1.9863 Soluble
203 C H 0.0000 Buried
204 N H -1.7048 Soluble
205 V H 0.0000 Buried
206 D H -1.8087 Soluble
207 H H 0.0000 Buried
208 K H -2.7191 Soluble
209 P H -1.5445 Soluble
210 S H -1.8059 Soluble
211 N H -2.4223 Soluble
212 T H -2.2131 Soluble
213 K H -2.7809 Soluble
214 V H -1.8256 Soluble
215 D H -2.8945 Soluble
216 K H -2.6921 Soluble
217 R H -3.0945 Soluble
218 V H 0.0000 Buried
219 E H -2.8902 Soluble
220 S H -1.8492 Soluble
221 K H -1.9866 Soluble
1 D L -1.5160 Soluble
2 I L -0.1908 Soluble
3 V L 0.7023 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.6833 Soluble
6 Q L 0.0000 Buried
7 S L -1.1721 Soluble
8 P L -1.3273 Soluble
9 D L -2.2944 Soluble
10 S L -1.4991 Soluble
11 L L -0.7933 Soluble
12 A L -0.6887 Soluble
13 V L 0.0000 Buried
14 S L -1.3124 Soluble
15 L L -0.4976 Soluble
16 G L -1.3311 Soluble
17 E L -2.7438 Soluble
18 R L -2.8898 Soluble
19 A L 0.0000 Buried
20 T L -0.7219 Soluble
21 I L 0.0000 Buried
22 N L -0.8980 Soluble
23 C L 0.0000 Buried
24 K L -1.9934 Soluble
25 S L 0.0000 Buried
26 S L -1.1197 Soluble
27 E L -2.0287 Soluble
28 S L -1.0955 Soluble
29 V L 0.0000 Buried
36 S L -0.2143 Soluble
37 N L 0.1425 Aggregation-prone
38 D L 0.3154 Aggregation-prone
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -2.1665 Soluble
46 P L -1.5920 Soluble
47 G L -1.4583 Soluble
48 Q L -2.0585 Soluble
49 P L -1.4706 Soluble
50 P L 0.0000 Buried
51 K L -1.7193 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 N L 0.0000 Buried
56 Y L 0.5290 Aggregation-prone
57 A L 0.0000 Buried
65 F L 1.4831 Aggregation-prone
66 H L 0.1231 Aggregation-prone
67 R L -0.7178 Soluble
68 F L -0.2666 Soluble
69 T L -0.5120 Soluble
70 G L -0.7462 Soluble
71 V L 0.0000 Buried
72 P L -1.2397 Soluble
74 D L -2.1559 Soluble
75 R L -1.6532 Soluble
76 F L 0.0000 Buried
77 S L -0.4555 Soluble
78 G L 0.2149 Aggregation-prone
79 S L 0.2895 Aggregation-prone
80 G L 0.1366 Aggregation-prone
83 Y L 0.8540 Aggregation-prone
84 G L -0.5688 Soluble
85 T L -1.3926 Soluble
86 D L -1.6944 Soluble
87 F L 0.0000 Buried
88 T L -0.3121 Soluble
89 L L 0.0000 Buried
90 T L -0.6363 Soluble
91 I L 0.0000 Buried
92 S L -1.8807 Soluble
93 S L -1.9088 Soluble
94 L L 0.0000 Buried
95 Q L -1.5949 Soluble
96 A L -1.6812 Soluble
97 E L -2.5664 Soluble
98 D L 0.0000 Buried
99 V L -1.3756 Soluble
100 A L 0.0000 Buried
101 V L -0.9464 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 H L 0.0000 Buried
106 Q L 0.0000 Buried
107 A L 0.6440 Aggregation-prone
108 Y L 1.0212 Aggregation-prone
109 S L 0.1996 Aggregation-prone
114 S L -0.3183 Soluble
115 P L 0.0000 Buried
116 Y L 0.0000 Buried
117 T L -0.2782 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.4263 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.9367 Soluble
124 L L 0.0000 Buried
125 E L -1.3445 Soluble
126 I L 0.0000 Buried
127 K L -1.6137 Soluble
128 R L -0.8030 Soluble
129 T L 0.3213 Aggregation-prone
130 V L 1.2670 Aggregation-prone
131 A L 0.5679 Aggregation-prone
132 A L 0.1960 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.0724 Soluble
135 V L 0.0000 Buried
136 F L 0.2896 Aggregation-prone
137 I L 0.2494 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6248 Soluble
140 P L 0.0000 Buried
141 S L -1.5963 Soluble
142 D L -2.9192 Soluble
143 E L -2.9941 Soluble
144 Q L 0.0000 Buried
145 L L -2.1268 Soluble
146 K L -2.8852 Soluble
147 S L -1.7458 Soluble
148 G L -1.2491 Soluble
149 T L -1.2832 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6346 Soluble
159 F L 0.0000 Buried
160 Y L -0.7252 Soluble
161 P L -1.5351 Soluble
162 R L -3.1324 Soluble
163 E L -3.2172 Soluble
164 A L -2.3658 Soluble
165 K L -2.4819 Soluble
166 V L -1.1941 Soluble
167 Q L -0.7187 Soluble
168 W L 0.0000 Buried
169 K L -0.6811 Soluble
170 V L 0.0000 Buried
171 D L -2.0924 Soluble
172 N L -1.6452 Soluble
173 A L -0.3992 Soluble
174 L L 0.5339 Aggregation-prone
175 Q L -0.5569 Soluble
176 S L -0.7687 Soluble
177 G L -1.3770 Soluble
178 N L -1.7756 Soluble
179 S L -1.5775 Soluble
180 Q L -1.5267 Soluble
181 E L -1.7037 Soluble
182 S L -0.7233 Soluble
183 V L -0.7574 Soluble
184 T L -1.0782 Soluble
185 E L -2.3123 Soluble
186 Q L -2.0518 Soluble
187 D L -1.7779 Soluble
188 S L -2.2913 Soluble
189 K L -2.6067 Soluble
190 D L -1.6919 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L -1.2370 Soluble
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7843 Soluble
199 L L 0.0000 Buried
200 T L -0.6570 Soluble
201 L L -0.9717 Soluble
202 S L -1.3156 Soluble
203 K L -2.3410 Soluble
204 A L -2.2065 Soluble
205 D L -3.0543 Soluble
206 Y L 0.0000 Buried
207 E L -4.0520 Soluble
208 K L -3.9697 Soluble
209 H L -3.5498 Soluble
210 K L -3.6927 Soluble
211 V L -2.1637 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8304 Soluble
216 V L 0.0000 Buried
217 T L -1.0932 Soluble
218 H L 0.0000 Buried
219 Q L -1.6291 Soluble
220 G L -0.1851 Soluble
221 L L -0.0750 Soluble
222 S L -0.3839 Soluble
223 S L -0.3890 Soluble
224 P L -0.6060 Soluble
225 V L -0.0336 Soluble
226 T L -0.5062 Soluble
227 K L -0.6903 Soluble
228 S L -0.8058 Soluble
229 F L -1.1091 Soluble
230 N L -2.5990 Soluble
231 R L -3.4459 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7701 2.8309 CSV PDB
4.5 -0.8173 2.8315 CSV PDB
5.0 -0.873 2.837 CSV PDB
5.5 -0.9254 2.8586 CSV PDB
6.0 -0.9612 2.9072 CSV PDB
6.5 -0.9713 2.9704 CSV PDB
7.0 -0.9553 3.0176 CSV PDB
7.5 -0.9215 3.0362 CSV PDB
8.0 -0.8775 3.0359 CSV PDB
8.5 -0.8263 3.0275 CSV PDB
9.0 -0.7686 3.0164 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH