Amlenetug / 8b9v

A4DAB013_8b9v_H_L | Source PDB: 8b9v | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7803
Max Score
1.2217
Min Score
-3.7522
Total Score
-330.87
Score CDR
-0.6724
Score non-CDR
-0.7928

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQTGGSLRLSCAASGFTFSSYAMTWVRQAPGKGLEWVSAIRSQGDRTDYADSVKGRFTISRDNSQNTLYLQMNSLRAEDTAVYYCAKNWAPFDSWGQGTLVTVSSASTKGPSVFPLAPSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7522
Maximum Score 1.2217
Average Score -0.7803
Total Score -330.8677

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9101 Soluble
2 V H -0.9084 Soluble
3 Q H -1.0088 Soluble
4 L H 0.0000 Buried
5 L H 0.6675 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4629 Soluble
8 G H -0.9099 Soluble
9 G H -0.5344 Soluble
11 G H -0.2583 Soluble
12 L H -0.0885 Soluble
13 V H 0.0000 Buried
14 Q H -1.5479 Soluble
15 T H -1.4128 Soluble
16 G H -1.5030 Soluble
17 G H -1.2396 Soluble
18 S H -1.4613 Soluble
19 L H -1.2971 Soluble
20 R H -2.1540 Soluble
21 L H 0.0000 Buried
22 S H -0.3967 Soluble
23 C H 0.0000 Buried
24 A H -0.0219 Soluble
25 A H 0.0000 Buried
26 S H -0.7124 Soluble
27 G H -1.0655 Soluble
28 F H -0.3498 Soluble
29 T H -0.1237 Soluble
30 F H 0.0000 Buried
35 S H -0.9032 Soluble
36 S H -0.4805 Soluble
37 Y H -0.0979 Soluble
38 A H 0.0000 Buried
39 M H 0.0000 Buried
40 T H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8251 Soluble
45 A H -1.1892 Soluble
46 P H -0.9684 Soluble
47 G H -1.4948 Soluble
48 K H -2.4238 Soluble
49 G H -1.6698 Soluble
50 L H 0.0000 Buried
51 E H -1.1674 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 A H 0.0000 Buried
56 I H 0.0000 Buried
57 R H -2.9576 Soluble
58 S H -1.9709 Soluble
59 Q H -2.9140 Soluble
62 G H -2.9982 Soluble
63 D H -3.6623 Soluble
64 R H -3.3910 Soluble
65 T H -1.7619 Soluble
66 D H -1.2531 Soluble
67 Y H -1.0387 Soluble
68 A H 0.0000 Buried
69 D H -2.4594 Soluble
70 S H -1.6871 Soluble
71 V H 0.0000 Buried
72 K H -2.6161 Soluble
74 G H -1.8320 Soluble
75 R H -1.5282 Soluble
76 F H 0.0000 Buried
77 T H -0.9683 Soluble
78 I H 0.0000 Buried
79 S H -0.9991 Soluble
80 R H -1.5758 Soluble
81 D H -1.8469 Soluble
82 N H -1.8679 Soluble
83 S H -1.4128 Soluble
84 Q H -1.9157 Soluble
85 N H -1.4670 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.4721 Soluble
89 L H 0.0000 Buried
90 Q H -1.3588 Soluble
91 M H 0.0000 Buried
92 N H -2.0574 Soluble
93 S H -1.4625 Soluble
94 L H 0.0000 Buried
95 R H -2.1398 Soluble
96 A H -1.5729 Soluble
97 E H -2.1322 Soluble
98 D H 0.0000 Buried
99 T H -0.6650 Soluble
100 A H 0.0000 Buried
101 V H 0.1949 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 K H 0.0000 Buried
107 N H -0.1375 Soluble
108 W H 0.2490 Aggregation-prone
109 A H 0.2722 Aggregation-prone
114 P H 0.1531 Aggregation-prone
115 F H 0.0000 Buried
116 D H -0.6966 Soluble
117 S H -0.5555 Soluble
118 W H -0.4571 Soluble
119 G H 0.0000 Buried
120 Q H -1.2332 Soluble
121 G H 0.0000 Buried
122 T H -0.0255 Soluble
123 L H 0.1472 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.4126 Soluble
126 V H 0.0000 Buried
127 S H -0.9372 Soluble
128 S H -0.8316 Soluble
129 A H -0.5573 Soluble
130 S H -0.7291 Soluble
131 T H -0.8163 Soluble
132 K H -1.6095 Soluble
133 G H -1.6811 Soluble
134 P H -0.7405 Soluble
135 S H -0.4736 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1471 Soluble
139 L H 0.0000 Buried
140 A H -1.2481 Soluble
141 P H 0.0000 Buried
142 S H -0.9381 Soluble
143 G H -0.7879 Soluble
144 G H -0.8447 Soluble
145 T H -0.5558 Soluble
146 A H -0.2809 Soluble
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.4617 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.4618 Soluble
159 P H -0.6819 Soluble
160 V H -0.6582 Soluble
161 T H -0.6009 Soluble
162 V H -0.1861 Soluble
163 S H -0.3970 Soluble
164 W H 0.0000 Buried
165 N H -0.8547 Soluble
166 S H -0.6841 Soluble
167 G H -0.5282 Soluble
168 A H -0.2287 Soluble
169 L H -0.0026 Soluble
170 T H -0.1685 Soluble
171 S H -0.1579 Soluble
172 G H -0.1727 Soluble
173 V H 0.1325 Aggregation-prone
174 H H -0.3306 Soluble
175 T H -0.1445 Soluble
176 F H 0.0000 Buried
177 P H -0.3460 Soluble
178 A H 0.2156 Aggregation-prone
179 V H 0.4392 Aggregation-prone
180 L H 1.2217 Aggregation-prone
181 Q H 0.2673 Aggregation-prone
182 S H -0.0973 Soluble
183 S H -0.2394 Soluble
184 G H -0.0369 Soluble
185 L H 0.0390 Aggregation-prone
186 Y H 0.4136 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.1817 Aggregation-prone
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.0426 Soluble
194 V H -0.2283 Soluble
195 P H -0.4960 Soluble
196 S H -0.5894 Soluble
197 S H -0.6253 Soluble
198 S H -0.6279 Soluble
199 L H -0.8555 Soluble
200 G H -1.0446 Soluble
201 T H -0.8597 Soluble
202 Q H -1.2631 Soluble
203 T H -1.2836 Soluble
204 Y H 0.0000 Buried
205 I H -1.5864 Soluble
206 C H 0.0000 Buried
207 N H -1.5749 Soluble
208 V H 0.0000 Buried
209 N H -1.9854 Soluble
210 H H 0.0000 Buried
211 K H -2.7812 Soluble
212 P H -1.6418 Soluble
213 S H -1.9462 Soluble
214 N H -2.6669 Soluble
215 T H -2.1658 Soluble
216 K H -2.7615 Soluble
217 V H -1.7460 Soluble
218 D H -2.7205 Soluble
219 K H -2.5289 Soluble
220 R H -2.8460 Soluble
221 V H 0.0000 Buried
222 E H -2.8269 Soluble
223 P H -1.8915 Soluble
224 K H -2.2646 Soluble
1 E L -1.7233 Soluble
2 I L 0.0000 Buried
3 V L 0.2528 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.8246 Soluble
6 Q L 0.0000 Buried
7 S L -0.8793 Soluble
8 P L -0.5134 Soluble
9 G L -0.8746 Soluble
10 T L -0.5468 Soluble
11 L L -0.3238 Soluble
12 S L -0.5683 Soluble
13 L L -0.9714 Soluble
14 S L -1.6068 Soluble
15 P L -2.0012 Soluble
16 G L -2.1587 Soluble
17 E L -2.7366 Soluble
18 R L -3.1465 Soluble
19 A L 0.0000 Buried
20 T L -0.6625 Soluble
21 L L 0.0000 Buried
22 S L -0.8881 Soluble
23 C L 0.0000 Buried
24 R L -2.3255 Soluble
25 A L -1.4386 Soluble
26 S L -1.1485 Soluble
27 Q L -1.8348 Soluble
28 S L -1.3880 Soluble
29 V L 0.0000 Buried
30 S L -0.3860 Soluble
36 S L -0.1669 Soluble
37 S L -0.1067 Soluble
38 Y L 0.6385 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.5544 Soluble
44 Q L 0.0000 Buried
45 K L -1.7081 Soluble
46 P L -1.4855 Soluble
47 G L -1.4897 Soluble
48 Q L -2.0371 Soluble
49 A L -1.2886 Soluble
50 P L 0.0000 Buried
51 R L -0.9784 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.3885 Aggregation-prone
56 G L 0.1290 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.4112 Soluble
66 S L -0.4393 Soluble
67 R L -1.1213 Soluble
68 A L -0.5428 Soluble
69 T L -0.5108 Soluble
70 G L -0.7794 Soluble
71 I L -0.8618 Soluble
72 P L -1.2441 Soluble
74 D L -2.1513 Soluble
75 R L -2.0484 Soluble
76 F L 0.0000 Buried
77 S L -0.8848 Soluble
78 G L -0.4587 Soluble
79 S L -0.6874 Soluble
80 G L -1.0677 Soluble
83 S L -0.9595 Soluble
84 G L -1.1075 Soluble
85 T L -1.6917 Soluble
86 D L -2.0752 Soluble
87 F L 0.0000 Buried
88 T L -0.7552 Soluble
89 L L 0.0000 Buried
90 T L -0.8084 Soluble
91 I L 0.0000 Buried
92 S L -2.3847 Soluble
93 R L -3.2795 Soluble
94 L L 0.0000 Buried
95 E L -2.5600 Soluble
96 P L -2.0209 Soluble
97 E L -2.3018 Soluble
98 D L 0.0000 Buried
99 F L -0.7695 Soluble
100 A L 0.0000 Buried
101 V L -0.3984 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 G L -0.1917 Soluble
109 S L -0.6560 Soluble
114 S L -0.6335 Soluble
115 P L -0.8441 Soluble
116 W L -0.1227 Soluble
117 T L -0.2190 Soluble
118 F L -0.0339 Soluble
119 G L 0.0000 Buried
120 Q L -1.5560 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.9049 Soluble
124 V L 0.0000 Buried
125 E L -0.8135 Soluble
126 I L -0.9406 Soluble
127 K L -1.4613 Soluble
128 R L -1.0493 Soluble
129 T L -0.1093 Soluble
130 V L 0.3794 Aggregation-prone
131 A L -0.0256 Soluble
132 A L -0.1091 Soluble
133 P L 0.0000 Buried
134 S L -0.2380 Soluble
135 V L 0.0000 Buried
136 F L 0.0845 Aggregation-prone
137 I L 0.1576 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5584 Soluble
140 P L 0.0000 Buried
141 S L -1.4390 Soluble
142 D L -2.7373 Soluble
143 E L -2.4632 Soluble
144 Q L 0.0000 Buried
145 L L -2.2234 Soluble
146 K L -2.7297 Soluble
147 S L -1.7199 Soluble
148 G L -1.4755 Soluble
149 T L -1.1678 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.3559 Soluble
158 N L -0.8209 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5492 Soluble
162 R L -2.4792 Soluble
163 E L -3.0026 Soluble
164 A L -2.2383 Soluble
165 K L -2.2104 Soluble
166 V L -1.1708 Soluble
167 Q L -0.6783 Soluble
168 W L 0.0000 Buried
169 K L -0.7833 Soluble
170 V L 0.0000 Buried
171 D L -2.1747 Soluble
172 N L -1.7238 Soluble
173 A L -0.5000 Soluble
174 L L 0.2474 Aggregation-prone
175 Q L -0.3563 Soluble
176 S L -0.5404 Soluble
177 G L -0.9119 Soluble
178 N L -0.8695 Soluble
179 S L -1.1070 Soluble
180 Q L -1.3177 Soluble
181 E L -1.9181 Soluble
182 S L -1.0370 Soluble
183 V L -0.8535 Soluble
184 T L -1.1531 Soluble
185 E L -2.3070 Soluble
186 Q L -1.8810 Soluble
187 D L -1.9740 Soluble
188 S L -2.0238 Soluble
189 K L -2.2864 Soluble
190 D L -1.7309 Soluble
191 S L -1.7595 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6196 Soluble
199 L L 0.0000 Buried
200 T L -0.3224 Soluble
201 L L -0.3887 Soluble
202 S L -1.0635 Soluble
203 K L -2.1317 Soluble
204 A L -1.8963 Soluble
205 D L -2.5188 Soluble
206 Y L 0.0000 Buried
207 E L -3.7522 Soluble
208 K L -3.7518 Soluble
209 H L -3.3266 Soluble
210 K L -3.5239 Soluble
211 V L -1.9306 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6668 Soluble
216 V L 0.0000 Buried
217 T L -1.2087 Soluble
218 H L 0.0000 Buried
219 Q L -1.6815 Soluble
220 G L -0.3952 Soluble
221 L L -0.2402 Soluble
222 S L -0.4574 Soluble
223 S L -0.4512 Soluble
224 P L -0.5884 Soluble
225 V L -0.0196 Soluble
226 T L -0.4410 Soluble
227 K L -0.6480 Soluble
228 S L -0.7395 Soluble
229 F L -1.0230 Soluble
230 N L -2.3261 Soluble
231 R L -2.8472 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.8106 1.646 CSV PDB
4.5 -0.8571 1.5762 CSV PDB
5.0 -0.9116 1.4821 CSV PDB
5.5 -0.9647 1.4524 CSV PDB
6.0 -1.0061 1.4289 CSV PDB
6.5 -1.0272 1.4073 CSV PDB
7.0 -1.0267 1.3892 CSV PDB
7.5 -1.0109 1.3744 CSV PDB
8.0 -0.9859 1.362 CSV PDB
8.5 -0.9536 1.491 CSV PDB
9.0 -0.9143 1.6501 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH