Umesolerbart / 7mxl

A4DAB281_7mxl_H_L | Source PDB: 7mxl | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6563
Max Score
1.8772
Min Score
-3.5277
Total Score
-282.23
Score CDR
-0.3351
Score non-CDR
-0.6948

Calculation Parameters

Chain Sequence(s) EVQLVESGGDLVQPGGSLRLSCAASGFTFSSYEMNWVRQAPGKGLEWVSFISDSSSNIYYADSVKGRFTISRDNAKKSLYLQMTSLRAEDTAVYYCAREAIGSTSFDNWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVIVMTQSPATLSVSPGERATLSCRASQSVSSSLAWYQQKPGQAPRRLIYSASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAIYYCHQYNNWPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5277
Maximum Score 1.8772
Average Score -0.6563
Total Score -282.2274

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.2388 Soluble
2 V H -1.6180 Soluble
3 Q H -1.5932 Soluble
4 L H 0.0000 Buried
5 V H 0.9532 Aggregation-prone
6 E H 0.1637 Aggregation-prone
7 S H -0.5324 Soluble
8 G H -0.7985 Soluble
9 G H -1.0513 Soluble
11 D H -1.5756 Soluble
12 L H -0.4705 Soluble
13 V H -0.9642 Soluble
14 Q H -1.7606 Soluble
15 P H -1.6959 Soluble
16 G H -1.4317 Soluble
17 G H -0.9477 Soluble
18 S H -0.8864 Soluble
19 L H -1.0513 Soluble
20 R H -1.7578 Soluble
21 L H 0.0000 Buried
22 S H -0.3953 Soluble
23 C H 0.0000 Buried
24 A H -0.2233 Soluble
25 A H 0.0000 Buried
26 S H -0.9784 Soluble
27 G H -1.4756 Soluble
28 F H 0.0000 Buried
29 T H -0.4075 Soluble
30 F H 0.0000 Buried
35 S H -0.7784 Soluble
36 S H -0.0909 Soluble
37 Y H 0.2114 Aggregation-prone
38 E H -0.3315 Soluble
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.6090 Soluble
44 Q H -0.9078 Soluble
45 A H -1.2930 Soluble
46 P H -0.8886 Soluble
47 G H -1.4485 Soluble
48 K H -2.2892 Soluble
49 G H -1.4172 Soluble
50 L H 0.0000 Buried
51 E H -1.1709 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 F H 0.0000 Buried
56 I H 0.0000 Buried
57 S H -0.6669 Soluble
58 D H -0.9613 Soluble
59 S H -0.8214 Soluble
62 S H -0.6537 Soluble
63 S H -0.7062 Soluble
64 N H -0.8642 Soluble
65 I H 0.3569 Aggregation-prone
66 Y H 0.2626 Aggregation-prone
67 Y H -0.4422 Soluble
68 A H 0.0000 Buried
69 D H -2.2749 Soluble
70 S H -1.5849 Soluble
71 V H 0.0000 Buried
72 K H -2.4272 Soluble
74 G H -1.6725 Soluble
75 R H -1.3912 Soluble
76 F H 0.0000 Buried
77 T H -0.6152 Soluble
78 I H 0.0000 Buried
79 S H -0.3096 Soluble
80 R H -1.1719 Soluble
81 D H -1.7982 Soluble
82 N H -1.8601 Soluble
83 A H -1.5116 Soluble
84 K H -2.4161 Soluble
85 K H -2.0149 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6115 Soluble
89 L H 0.0000 Buried
90 Q H -1.0498 Soluble
91 M H 0.0000 Buried
92 T H -0.9433 Soluble
93 S H -1.0856 Soluble
94 L H 0.0000 Buried
95 R H -2.3541 Soluble
96 A H -1.7986 Soluble
97 E H -2.2411 Soluble
98 D H 0.0000 Buried
99 T H -0.7084 Soluble
100 A H 0.0000 Buried
101 V H 0.0729 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 E H 0.0000 Buried
108 A H 0.6526 Aggregation-prone
109 I H 1.6825 Aggregation-prone
110 G H 0.4611 Aggregation-prone
112 S H 0.0996 Aggregation-prone
113 T H 0.1117 Aggregation-prone
114 S H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.4968 Soluble
117 N H -1.1149 Soluble
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -1.2314 Soluble
121 G H -0.4931 Soluble
122 T H -0.2446 Soluble
123 L H -0.0397 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.9585 Soluble
128 S H -0.6727 Soluble
129 A H -0.4945 Soluble
130 S H -0.5845 Soluble
131 T H -0.6782 Soluble
132 K H -1.1731 Soluble
133 G H -1.2257 Soluble
134 P H -0.5319 Soluble
135 S H -0.1825 Soluble
136 V H 0.0000 Buried
137 F H -0.6586 Soluble
138 P H -0.6103 Soluble
139 L H 0.0000 Buried
140 A H 0.4734 Aggregation-prone
141 P H 0.1494 Aggregation-prone
142 C H -0.1202 Soluble
143 S H -0.4509 Soluble
144 R H -0.9253 Soluble
145 S H -0.9032 Soluble
146 T H -0.9344 Soluble
147 S H -1.4006 Soluble
148 E H -2.1023 Soluble
149 S H -1.2504 Soluble
150 T H -0.8786 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.3198 Soluble
159 D H -0.5109 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.9346 Soluble
164 P H -1.0248 Soluble
165 V H 0.0000 Buried
166 T H -0.6851 Soluble
167 V H 0.0000 Buried
168 S H -0.5626 Soluble
169 W H 0.0000 Buried
170 N H -0.9140 Soluble
171 S H -0.7321 Soluble
172 G H -0.5477 Soluble
173 A H -0.2122 Soluble
174 L H -0.0090 Soluble
175 T H -0.1484 Soluble
176 S H -0.1428 Soluble
177 G H -0.1928 Soluble
178 V H 0.1896 Aggregation-prone
179 H H -0.2970 Soluble
180 T H -0.2373 Soluble
181 F H 0.0000 Buried
182 P H -0.4029 Soluble
183 A H 0.2353 Aggregation-prone
184 V H 0.3746 Aggregation-prone
185 L H 1.1912 Aggregation-prone
186 Q H 0.1839 Aggregation-prone
187 S H -0.1057 Soluble
188 S H -0.1769 Soluble
189 G H -0.0177 Soluble
190 L H -0.0904 Soluble
191 Y H 0.0000 Buried
192 S H 0.0740 Aggregation-prone
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.0867 Soluble
199 V H 0.0000 Buried
200 P H -0.5140 Soluble
201 S H -0.5852 Soluble
202 S H -0.4815 Soluble
203 S H -0.6023 Soluble
204 L H -0.0273 Soluble
205 G H -0.6573 Soluble
206 T H -0.9453 Soluble
207 K H -1.6520 Soluble
208 T H -1.1130 Soluble
209 Y H 0.0000 Buried
210 T H -1.5128 Soluble
211 C H 0.0000 Buried
212 N H -1.5644 Soluble
213 V H 0.0000 Buried
214 D H -1.9083 Soluble
215 H H 0.0000 Buried
216 K H -2.8030 Soluble
217 P H -1.7007 Soluble
218 S H -1.8608 Soluble
219 N H -2.4286 Soluble
220 T H -1.8202 Soluble
221 K H -2.2683 Soluble
222 V H -0.7935 Soluble
223 D H -2.0050 Soluble
224 K H -1.6183 Soluble
225 R H -2.0462 Soluble
226 V H -0.2806 Soluble
2 I L 1.3735 Aggregation-prone
3 V L 1.8772 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.0965 Soluble
6 Q L 0.0000 Buried
7 S L -0.5137 Soluble
8 P L -0.2394 Soluble
9 A L -0.1820 Soluble
10 T L -0.1758 Soluble
11 L L 0.0469 Aggregation-prone
12 S L -0.4818 Soluble
13 V L -0.9477 Soluble
14 S L -1.3929 Soluble
15 P L -1.6487 Soluble
16 G L -1.9957 Soluble
17 E L -2.7242 Soluble
18 R L -2.8068 Soluble
19 A L 0.0000 Buried
20 T L -0.6172 Soluble
21 L L 0.0000 Buried
22 S L -0.5618 Soluble
23 C L 0.0000 Buried
24 R L -1.3994 Soluble
25 A L 0.0000 Buried
26 S L -0.0795 Soluble
27 Q L -1.0615 Soluble
28 S L -1.0781 Soluble
29 V L -0.8449 Soluble
36 S L -0.7717 Soluble
37 S L -0.6536 Soluble
38 S L -0.5801 Soluble
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.4033 Soluble
46 P L -1.2916 Soluble
47 G L -1.4750 Soluble
48 Q L -2.0449 Soluble
49 A L -1.4500 Soluble
50 P L 0.0000 Buried
51 R L -1.3323 Soluble
52 R L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.1885 Soluble
56 S L -0.4805 Soluble
57 A L 0.0000 Buried
65 S L -0.6609 Soluble
66 T L -0.6360 Soluble
67 R L -1.1707 Soluble
68 A L 0.0000 Buried
69 T L -0.6080 Soluble
70 G L -0.6635 Soluble
71 I L 0.0000 Buried
72 P L -0.4141 Soluble
74 A L -0.4020 Soluble
75 R L -0.7261 Soluble
76 F L 0.0000 Buried
77 S L -0.5751 Soluble
78 G L -0.5723 Soluble
79 S L -0.6474 Soluble
80 G L -0.9885 Soluble
83 S L -0.9424 Soluble
84 G L -1.0147 Soluble
85 T L -1.3401 Soluble
86 E L -1.5900 Soluble
87 F L 0.0000 Buried
88 T L -0.5823 Soluble
89 L L 0.0000 Buried
90 T L -0.6286 Soluble
91 I L 0.0000 Buried
92 S L -1.5875 Soluble
93 S L -1.6687 Soluble
94 L L 0.0000 Buried
95 Q L -1.9465 Soluble
96 S L -1.1913 Soluble
97 E L -1.4623 Soluble
98 D L 0.0000 Buried
99 F L -0.3468 Soluble
100 A L 0.0000 Buried
101 I L 0.0436 Aggregation-prone
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 H L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 N L -1.2512 Soluble
109 N L -1.3564 Soluble
114 W L -0.5302 Soluble
115 P L -0.4794 Soluble
116 L L 0.0000 Buried
117 T L 0.1288 Aggregation-prone
118 F L 0.0000 Buried
119 G L -0.2983 Soluble
120 G L -0.7469 Soluble
121 G L -0.5826 Soluble
122 T L 0.0000 Buried
123 K L -0.2857 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.6246 Soluble
127 K L -1.2002 Soluble
128 R L -0.9581 Soluble
129 T L 0.1503 Aggregation-prone
130 V L 1.0716 Aggregation-prone
131 A L 0.3158 Aggregation-prone
132 A L -0.0373 Soluble
133 P L 0.0000 Buried
134 S L -0.2288 Soluble
135 V L 0.0000 Buried
136 F L 0.1278 Aggregation-prone
137 I L 0.0780 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.4375 Soluble
140 P L -0.5873 Soluble
141 S L -1.4504 Soluble
142 D L -2.8468 Soluble
143 E L -3.1549 Soluble
144 Q L 0.0000 Buried
145 L L -2.2822 Soluble
146 K L -2.8017 Soluble
147 S L -1.8428 Soluble
148 G L -1.2473 Soluble
149 T L -1.0288 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -1.5257 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3331 Soluble
162 R L -2.5541 Soluble
163 E L -2.9112 Soluble
164 A L -2.2439 Soluble
165 K L -2.5365 Soluble
166 V L 0.0000 Buried
167 Q L -0.6868 Soluble
168 W L 0.0000 Buried
169 K L -0.7867 Soluble
170 V L 0.0000 Buried
171 D L -2.3820 Soluble
172 N L -1.7849 Soluble
173 A L -0.5513 Soluble
174 L L 0.5690 Aggregation-prone
175 Q L -0.0985 Soluble
176 S L -0.5176 Soluble
177 G L -0.8964 Soluble
178 N L -0.7913 Soluble
179 S L -1.1988 Soluble
180 Q L -1.4953 Soluble
181 E L -2.0308 Soluble
182 S L 0.0000 Buried
183 V L -0.8007 Soluble
184 T L -1.0897 Soluble
185 E L -2.2319 Soluble
186 Q L -2.0966 Soluble
187 D L -2.5086 Soluble
188 S L -2.0756 Soluble
189 K L -2.9122 Soluble
190 D L -3.1102 Soluble
191 S L -2.3922 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6827 Soluble
199 L L 0.0000 Buried
200 T L -0.2701 Soluble
201 L L -0.3880 Soluble
202 S L -1.0358 Soluble
203 K L -2.1174 Soluble
204 A L -1.9202 Soluble
205 D L -2.6583 Soluble
206 Y L 0.0000 Buried
207 E L -3.5277 Soluble
208 K L -3.4564 Soluble
209 H L -2.8755 Soluble
210 K L -2.5724 Soluble
211 V L -1.4930 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8120 Soluble
216 V L 0.0000 Buried
217 T L -1.2735 Soluble
218 H L 0.0000 Buried
219 Q L -1.5876 Soluble
220 G L -0.2565 Soluble
221 L L -0.1958 Soluble
222 S L -0.4922 Soluble
223 S L -0.4440 Soluble
224 P L -0.6912 Soluble
225 V L -0.1271 Soluble
226 T L -0.5147 Soluble
227 K L -0.8065 Soluble
228 S L -0.6748 Soluble
229 F L 0.0000 Buried
230 N L -1.8181 Soluble
231 R L -1.9858 Soluble
232 G L -1.9369 Soluble
233 E L -1.9408 Soluble
234 C L -0.7351 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6794 2.7461 CSV PDB
4.5 -0.7246 2.7257 CSV PDB
5.0 -0.7774 2.701 CSV PDB
5.5 -0.8279 2.6747 CSV PDB
6.0 -0.8655 2.6494 CSV PDB
6.5 -0.8821 2.6277 CSV PDB
7.0 -0.8783 2.6113 CSV PDB
7.5 -0.8612 2.5995 CSV PDB
8.0 -0.837 2.5915 CSV PDB
8.5 -0.8071 2.587 CSV PDB
9.0 -0.771 2.5849 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH