Lampalizumab / 4d9q

A4DAB150_4d9q_H_L | Source PDB: 4d9q | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7215
Max Score
1.1525
Min Score
-3.5714
Total Score
-309.51
Score CDR
-0.7017
Score non-CDR
-0.7236

Calculation Parameters

Chain Sequence(s) EVQLVQSGPELKKPGASVKVSCKASGYTFTNYGMNWVRQAPGQGLEWMGWINTYTGETTYADDFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCEREGGVNNWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQVTQSPSSLSASVGDRVTITCITSTDIDDDMNWYQQKPGKVPKLLISGGNTLRPGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCLQSDSLPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5714
Maximum Score 1.1525
Average Score -0.7215
Total Score -309.5086

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.2745 Soluble
2 V H 0.0000 Buried
3 Q H -1.6550 Soluble
4 L H 0.0000 Buried
5 V H 0.7695 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.2833 Soluble
8 G H -0.5628 Soluble
9 P H -0.6718 Soluble
11 E H -1.0859 Soluble
12 L H -0.7742 Soluble
13 K H -1.6460 Soluble
14 K H -2.4056 Soluble
15 P H -2.0202 Soluble
16 G H -1.4357 Soluble
17 A H -1.1764 Soluble
18 S H -1.2422 Soluble
19 V H 0.0000 Buried
20 K H -1.8119 Soluble
21 V H 0.0000 Buried
22 S H -0.4378 Soluble
23 C H 0.0000 Buried
24 K H -0.2686 Soluble
25 A H 0.0000 Buried
26 S H -0.8359 Soluble
27 G H -1.4249 Soluble
28 Y H -0.7806 Soluble
29 T H -0.4650 Soluble
30 F H 0.0000 Buried
35 T H -0.1186 Soluble
36 N H -0.9418 Soluble
37 Y H -0.3688 Soluble
38 G H 0.0000 Buried
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.5310 Soluble
44 Q H -0.7214 Soluble
45 A H -1.0228 Soluble
46 P H -1.0196 Soluble
47 G H -1.2126 Soluble
48 Q H -1.8579 Soluble
49 G H -1.3913 Soluble
50 L H 0.0000 Buried
51 E H -1.2325 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 W H -0.1095 Soluble
56 I H 0.0000 Buried
57 N H -0.6284 Soluble
58 T H 0.0000 Buried
59 Y H 0.6453 Aggregation-prone
62 T H -0.1976 Soluble
63 G H -0.8342 Soluble
64 E H -1.8444 Soluble
65 T H -0.7548 Soluble
66 T H -0.4568 Soluble
67 Y H -0.8222 Soluble
68 A H -1.9764 Soluble
69 D H -3.5714 Soluble
70 D H -3.2928 Soluble
71 F H 0.0000 Buried
72 K H -2.9111 Soluble
74 G H -1.8241 Soluble
75 R H -1.1718 Soluble
76 F H 0.0000 Buried
77 V H 0.3074 Aggregation-prone
78 F H 0.0000 Buried
79 S H -0.3461 Soluble
80 L H 0.1793 Aggregation-prone
81 D H -0.3552 Soluble
82 T H 0.1670 Aggregation-prone
83 S H 0.2774 Aggregation-prone
84 V H 1.0020 Aggregation-prone
85 S H 0.1864 Aggregation-prone
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.3733 Soluble
89 L H 0.0000 Buried
90 Q H -0.8813 Soluble
91 I H 0.0000 Buried
92 S H -0.8435 Soluble
93 S H -1.1161 Soluble
94 L H 0.0000 Buried
95 K H -2.1757 Soluble
96 A H -1.7578 Soluble
97 E H -2.1894 Soluble
98 D H 0.0000 Buried
99 T H -0.8378 Soluble
100 A H 0.0000 Buried
101 V H 0.1961 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 E H 0.0000 Buried
106 R H 0.0000 Buried
107 E H -0.5559 Soluble
108 G H -0.3803 Soluble
114 G H 0.1717 Aggregation-prone
115 V H 1.1525 Aggregation-prone
116 N H -0.1732 Soluble
117 N H -0.6170 Soluble
118 W H -0.5299 Soluble
119 G H 0.0000 Buried
120 Q H -0.9901 Soluble
121 G H -0.3439 Soluble
122 T H 0.0000 Buried
123 L H -0.0623 Soluble
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.2947 Soluble
128 S H -0.9815 Soluble
129 A H -0.6744 Soluble
130 S H -0.7982 Soluble
131 T H -0.8971 Soluble
132 K H -1.6093 Soluble
133 G H -1.6868 Soluble
134 P H -0.8792 Soluble
135 S H -0.5411 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0087 Soluble
139 L H 0.0000 Buried
140 A H -1.2265 Soluble
141 P H 0.0000 Buried
142 S H -1.1026 Soluble
143 S H -0.8848 Soluble
144 K H -1.3192 Soluble
145 S H 0.0000 Buried
146 T H -0.8468 Soluble
147 S H -0.7633 Soluble
148 G H -0.9504 Soluble
149 G H -0.8731 Soluble
150 T H -0.5696 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.7931 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4273 Soluble
164 P H -0.7056 Soluble
165 V H -0.7307 Soluble
166 T H -0.6911 Soluble
167 V H -0.2684 Soluble
168 S H -0.4382 Soluble
169 W H 0.0000 Buried
170 N H -0.6745 Soluble
171 S H -0.6270 Soluble
172 G H -0.5370 Soluble
173 A H -0.2702 Soluble
174 L H -0.0370 Soluble
175 T H -0.1972 Soluble
176 S H -0.2002 Soluble
177 G H -0.2319 Soluble
178 V H 0.1438 Aggregation-prone
179 H H -0.3236 Soluble
180 T H -0.0112 Soluble
181 F H 0.0000 Buried
182 P H -0.4301 Soluble
183 A H 0.1578 Aggregation-prone
184 V H 0.3911 Aggregation-prone
185 L H 0.8429 Aggregation-prone
186 Q H 0.0977 Aggregation-prone
187 S H -0.2082 Soluble
188 S H -0.3014 Soluble
189 G H -0.0543 Soluble
190 L H -0.0836 Soluble
191 Y H 0.2363 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1382 Soluble
199 V H 0.0000 Buried
200 P H -0.5680 Soluble
201 S H -0.5656 Soluble
202 S H -0.5448 Soluble
203 S H -0.5594 Soluble
204 L H -0.7189 Soluble
205 G H -0.9376 Soluble
206 T H -0.6423 Soluble
207 Q H -1.1339 Soluble
208 T H -0.9774 Soluble
209 Y H 0.0000 Buried
210 I H -0.9670 Soluble
211 C H 0.0000 Buried
212 N H -1.4527 Soluble
213 V H 0.0000 Buried
214 N H -2.3741 Soluble
215 H H 0.0000 Buried
216 K H -2.9351 Soluble
217 P H -1.7861 Soluble
218 S H -1.9844 Soluble
219 N H -2.7129 Soluble
220 T H -2.2837 Soluble
221 K H -2.8846 Soluble
222 V H -1.7740 Soluble
223 D H -2.2136 Soluble
224 K H -1.8550 Soluble
225 K H -2.1734 Soluble
226 V H 0.0000 Buried
227 E H -2.8017 Soluble
228 P H -1.9709 Soluble
229 K H -2.4663 Soluble
1 D L -2.4067 Soluble
2 I L 0.0000 Buried
3 Q L -1.6100 Soluble
4 V L 0.0000 Buried
5 T L -0.5580 Soluble
6 Q L 0.0000 Buried
7 S L -0.5034 Soluble
8 P L -0.6483 Soluble
9 S L -0.9016 Soluble
10 S L -0.9102 Soluble
11 L L -0.6815 Soluble
12 S L -1.1921 Soluble
13 A L -1.1861 Soluble
14 S L -1.0415 Soluble
15 V L -0.0983 Soluble
16 G L -0.9306 Soluble
17 D L -1.8838 Soluble
18 R L -2.4443 Soluble
19 V L 0.0000 Buried
20 T L -0.6595 Soluble
21 I L 0.0000 Buried
22 T L -0.3190 Soluble
23 C L 0.0000 Buried
24 I L -0.3415 Soluble
25 T L 0.0000 Buried
26 S L -1.2585 Soluble
27 T L -1.7967 Soluble
28 D L -2.8840 Soluble
29 I L 0.0000 Buried
36 D L -3.3803 Soluble
37 D L -2.8020 Soluble
38 D L -2.4642 Soluble
39 M L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.3856 Soluble
45 K L -1.9198 Soluble
46 P L -1.5702 Soluble
47 G L -1.6093 Soluble
48 K L -2.2695 Soluble
49 V L -1.1688 Soluble
50 P L 0.0000 Buried
51 K L -1.9479 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 S L 0.0000 Buried
56 G L -1.3815 Soluble
57 G L -1.4227 Soluble
65 N L -0.9386 Soluble
66 T L -0.3779 Soluble
67 L L -0.0159 Soluble
68 R L -0.1628 Soluble
69 P L -0.2844 Soluble
70 G L -0.5697 Soluble
71 V L -0.4419 Soluble
72 P L -0.3757 Soluble
74 S L -0.4612 Soluble
75 R L -0.7676 Soluble
76 F L 0.0000 Buried
77 S L -0.3411 Soluble
78 G L -0.4428 Soluble
79 S L -0.9078 Soluble
80 G L -1.4178 Soluble
83 S L -2.1855 Soluble
84 G L -2.2907 Soluble
85 T L -1.8985 Soluble
86 D L -2.1564 Soluble
87 F L 0.0000 Buried
88 T L -0.5532 Soluble
89 L L 0.0000 Buried
90 T L -0.6455 Soluble
91 I L 0.0000 Buried
92 S L -1.4784 Soluble
93 S L -1.2795 Soluble
94 L L 0.0000 Buried
95 Q L -1.1126 Soluble
96 P L -1.4480 Soluble
97 E L -1.5545 Soluble
98 D L 0.0000 Buried
99 V L 0.0000 Buried
100 A L 0.0000 Buried
101 T L -1.0885 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 S L -0.9326 Soluble
108 D L -1.8794 Soluble
109 S L -0.5752 Soluble
114 L L 0.1879 Aggregation-prone
115 P L 0.0000 Buried
116 Y L -0.3117 Soluble
117 T L -0.7749 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.7078 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.8734 Soluble
124 V L 0.0000 Buried
125 E L -1.6395 Soluble
126 I L 0.0000 Buried
127 K L -1.6389 Soluble
128 R L -1.0116 Soluble
129 T L -0.1856 Soluble
130 V L 0.2359 Aggregation-prone
131 A L 0.1424 Aggregation-prone
132 A L -0.0980 Soluble
133 P L 0.0000 Buried
134 S L -0.1496 Soluble
135 V L 0.0000 Buried
136 F L 0.0000 Buried
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.4675 Soluble
140 P L 0.0000 Buried
141 S L -1.6532 Soluble
142 D L -2.8858 Soluble
143 E L -2.5429 Soluble
144 Q L 0.0000 Buried
145 L L -2.1232 Soluble
146 K L -2.6963 Soluble
147 S L -1.6648 Soluble
148 G L -1.2048 Soluble
149 T L -0.9775 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8183 Soluble
159 F L 0.0000 Buried
160 Y L -1.1035 Soluble
161 P L -1.7760 Soluble
162 R L -3.0447 Soluble
163 E L -3.1410 Soluble
164 A L -2.1585 Soluble
165 K L -2.0881 Soluble
166 V L 0.0000 Buried
167 Q L -0.4184 Soluble
168 W L 0.0000 Buried
169 K L -0.4951 Soluble
170 V L 0.0000 Buried
171 D L -1.8335 Soluble
172 N L -1.5368 Soluble
173 A L -0.2419 Soluble
174 L L 0.8010 Aggregation-prone
175 Q L 0.0156 Aggregation-prone
176 S L -0.3389 Soluble
177 G L -0.8316 Soluble
178 N L -0.7187 Soluble
179 S L -1.0496 Soluble
180 Q L -1.3314 Soluble
181 E L -2.0490 Soluble
182 S L -0.9901 Soluble
183 V L -0.8289 Soluble
184 T L -1.1801 Soluble
185 E L -2.3678 Soluble
186 Q L 0.0000 Buried
187 D L -2.0032 Soluble
188 S L -2.0773 Soluble
189 K L -2.4154 Soluble
190 D L -1.6465 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6556 Soluble
199 L L 0.0000 Buried
200 T L -0.4022 Soluble
201 L L -0.6266 Soluble
202 S L -1.2061 Soluble
203 K L -2.0196 Soluble
204 A L -1.8547 Soluble
205 D L -2.5572 Soluble
206 Y L 0.0000 Buried
207 E L -3.2480 Soluble
208 K L -3.4551 Soluble
209 H L -3.0118 Soluble
210 K L -3.3043 Soluble
211 V L -1.7365 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.5388 Soluble
216 V L 0.0000 Buried
217 T L -1.0691 Soluble
218 H L 0.0000 Buried
219 Q L -1.5762 Soluble
220 G L -0.4328 Soluble
221 L L -0.2153 Soluble
222 S L -0.4522 Soluble
223 S L -0.4102 Soluble
224 P L -0.5027 Soluble
225 V L 0.1435 Aggregation-prone
226 T L -0.2354 Soluble
227 K L -0.4407 Soluble
228 S L -0.4401 Soluble
229 F L -0.9965 Soluble
230 N L -1.9914 Soluble
231 R L -2.5357 Soluble
232 G L -2.1092 Soluble
233 E L -2.5111 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6797 1.8128 CSV PDB
4.5 -0.7336 1.8128 CSV PDB
5.0 -0.7951 1.8128 CSV PDB
5.5 -0.8531 1.8128 CSV PDB
6.0 -0.8954 1.8128 CSV PDB
6.5 -0.9127 1.8128 CSV PDB
7.0 -0.9048 1.8128 CSV PDB
7.5 -0.8805 1.8128 CSV PDB
8.0 -0.8469 1.8128 CSV PDB
8.5 -0.8058 1.8128 CSV PDB
9.0 -0.7559 1.8128 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH