Serplulimab / 7e9b

A4DAB238_7e9b_H_L | Source PDB: 7e9b | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6768
Max Score
1.8646
Min Score
-3.3889
Total Score
-284.26
Score CDR
-0.149
Score non-CDR
-0.737

Calculation Parameters

Chain Sequence(s) QVQLVEGGGLVKPGGSLRLSCAASGFTFSNYGMSWIRQAPGKGLEWVSTISGGGSNIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCVSYYYGIDFWGQGTSVTVSSASTKGPSVFPLAPESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVEDIQMTQSPSSLSASVGDRVTITCKASQDVTTAVAWYQQKPGKAPKLLIYWASTRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHYTIPWTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3889
Maximum Score 1.8646
Average Score -0.6768
Total Score -284.2625

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.3998 Soluble
2 V H -0.6697 Soluble
3 Q H -0.7221 Soluble
4 L H 0.0000 Buried
5 V H 0.8159 Aggregation-prone
6 E H -0.1286 Soluble
8 G H -1.0156 Soluble
9 G H -0.6506 Soluble
11 G H -0.1042 Soluble
12 L H 0.2651 Aggregation-prone
13 V H -0.6807 Soluble
14 K H -2.0569 Soluble
15 P H -2.0255 Soluble
16 G H -1.6754 Soluble
17 G H -1.3068 Soluble
18 S H -1.2337 Soluble
19 L H -0.8413 Soluble
20 R H -1.8116 Soluble
21 L H 0.0000 Buried
22 S H -0.5541 Soluble
23 C H 0.0000 Buried
24 A H -0.2876 Soluble
25 A H 0.0000 Buried
26 S H -0.8389 Soluble
27 G H -0.9504 Soluble
28 F H -0.4082 Soluble
29 T H -0.4716 Soluble
30 F H 0.0000 Buried
35 S H -1.1209 Soluble
36 N H -1.2287 Soluble
37 Y H 0.0877 Aggregation-prone
38 G H 0.0656 Aggregation-prone
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 I H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.9066 Soluble
45 A H -1.2959 Soluble
46 P H -1.0440 Soluble
47 G H -1.5112 Soluble
48 K H -2.2841 Soluble
49 G H -1.4355 Soluble
50 L H 0.0000 Buried
51 E H -1.0176 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 T H 0.0000 Buried
56 I H 0.0000 Buried
57 S H -0.5501 Soluble
58 G H -0.9723 Soluble
59 G H -1.2410 Soluble
62 G H -0.8175 Soluble
63 S H -0.7174 Soluble
64 N H -0.8893 Soluble
65 I H 0.5269 Aggregation-prone
66 Y H 0.4438 Aggregation-prone
67 Y H -0.3025 Soluble
68 A H 0.0000 Buried
69 D H -2.5940 Soluble
70 S H -1.6831 Soluble
71 V H 0.0000 Buried
72 K H -2.4796 Soluble
74 G H -1.6722 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.6091 Soluble
78 I H 0.0000 Buried
79 S H -0.3849 Soluble
80 R H -1.2351 Soluble
81 D H -1.9419 Soluble
82 N H -2.0121 Soluble
83 A H -1.4931 Soluble
84 K H -2.4278 Soluble
85 N H -1.9146 Soluble
86 S H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.0668 Soluble
91 M H 0.0000 Buried
92 N H -1.4866 Soluble
93 S H -1.4018 Soluble
94 L H 0.0000 Buried
95 R H -2.5536 Soluble
96 A H -1.8932 Soluble
97 E H -2.2744 Soluble
98 D H 0.0000 Buried
99 T H -0.8168 Soluble
100 A H 0.0000 Buried
101 V H -0.0960 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 V H 0.0000 Buried
106 S H 0.0000 Buried
107 Y H 1.2298 Aggregation-prone
108 Y H 1.8646 Aggregation-prone
109 Y H 1.4985 Aggregation-prone
114 G H 0.0000 Buried
115 I H 0.0000 Buried
116 D H 0.4806 Aggregation-prone
117 F H 0.6083 Aggregation-prone
118 W H -0.0881 Soluble
119 G H 0.0000 Buried
120 Q H -1.4467 Soluble
121 G H -0.8225 Soluble
122 T H -0.4433 Soluble
123 S H -0.3856 Soluble
124 V H 0.0000 Buried
125 T H -0.5635 Soluble
126 V H 0.0000 Buried
127 S H -1.0903 Soluble
128 S H -0.8556 Soluble
129 A H -0.5567 Soluble
130 S H -0.6009 Soluble
131 T H -0.6385 Soluble
132 K H -1.1745 Soluble
133 G H -1.3336 Soluble
134 P H 0.0000 Buried
135 S H -0.4469 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.3535 Soluble
139 L H 0.0000 Buried
140 A H -0.4952 Soluble
141 P H -0.4195 Soluble
142 E H -2.0058 Soluble
143 S H -1.2617 Soluble
144 T H -0.7943 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.3868 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.6627 Soluble
157 E H -0.9912 Soluble
158 P H -1.0178 Soluble
159 V H -0.7476 Soluble
160 T H -0.5863 Soluble
161 V H -0.3119 Soluble
162 S H -0.4574 Soluble
163 W H 0.0000 Buried
164 N H -0.9734 Soluble
165 S H -0.7609 Soluble
166 G H -0.5939 Soluble
167 A H -0.2809 Soluble
168 L H -0.1418 Soluble
169 T H -0.2263 Soluble
170 S H -0.2236 Soluble
171 G H -0.2422 Soluble
172 V H 0.1412 Aggregation-prone
173 H H -0.2924 Soluble
174 T H 0.0155 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.4347 Soluble
177 A H 0.1028 Aggregation-prone
178 V H 0.4702 Aggregation-prone
179 L H 1.1742 Aggregation-prone
180 Q H 0.3125 Aggregation-prone
181 S H -0.0326 Soluble
182 S H -0.1938 Soluble
183 G H 0.0564 Aggregation-prone
184 L H 0.0503 Aggregation-prone
185 Y H 0.3557 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1420 Soluble
193 V H 0.0000 Buried
194 P H -0.5085 Soluble
195 S H -0.5754 Soluble
196 S H -0.5963 Soluble
197 S H -0.4372 Soluble
198 L H -0.1629 Soluble
199 G H -0.7545 Soluble
200 T H -0.7944 Soluble
201 K H -1.6064 Soluble
202 T H -1.4107 Soluble
203 Y H 0.0000 Buried
204 T H -1.7612 Soluble
205 C H 0.0000 Buried
206 N H -1.6385 Soluble
207 V H 0.0000 Buried
208 D H -1.8375 Soluble
209 H H 0.0000 Buried
210 K H -2.8098 Soluble
211 P H -1.7422 Soluble
212 S H -1.8615 Soluble
213 N H -2.6023 Soluble
214 T H -2.0749 Soluble
215 K H -2.6230 Soluble
216 V H -1.7265 Soluble
217 D H -2.7490 Soluble
218 K H -2.3651 Soluble
219 R H -2.9424 Soluble
220 V H 0.0000 Buried
221 E H -2.4220 Soluble
1 D L -2.2788 Soluble
2 I L 0.0000 Buried
3 Q L -2.0188 Soluble
4 M L 0.0000 Buried
5 T L -1.0152 Soluble
6 Q L 0.0000 Buried
7 S L -0.5730 Soluble
8 P L -0.4362 Soluble
9 S L -0.6894 Soluble
10 S L -0.6727 Soluble
11 L L -0.2757 Soluble
12 S L -0.6423 Soluble
13 A L 0.0000 Buried
14 S L -0.3092 Soluble
15 V L 0.4701 Aggregation-prone
16 G L -0.5761 Soluble
17 D L -1.3457 Soluble
18 R L -2.1421 Soluble
19 V L 0.0000 Buried
20 T L -0.5412 Soluble
21 I L 0.0000 Buried
22 T L -0.7104 Soluble
23 C L 0.0000 Buried
24 K L -2.1990 Soluble
25 A L 0.0000 Buried
26 S L -2.0987 Soluble
27 Q L -2.6316 Soluble
28 D L -2.5701 Soluble
29 V L 0.0000 Buried
36 T L -0.7805 Soluble
37 T L -0.2976 Soluble
38 A L 0.0000 Buried
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6460 Soluble
46 P L -1.4825 Soluble
47 G L -1.6996 Soluble
48 K L -2.5809 Soluble
49 A L -1.5996 Soluble
50 P L 0.0000 Buried
51 K L -1.3002 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.4483 Aggregation-prone
56 W L 0.7484 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.3876 Soluble
66 T L -0.3924 Soluble
67 R L -1.1645 Soluble
68 H L -0.8771 Soluble
69 T L -0.6036 Soluble
70 G L -0.6521 Soluble
71 V L -0.6403 Soluble
72 P L -0.5119 Soluble
74 S L -0.6564 Soluble
75 R L -0.8770 Soluble
76 F L 0.0000 Buried
77 S L -0.6041 Soluble
78 G L 0.0000 Buried
79 S L -0.5835 Soluble
80 G L -1.0390 Soluble
83 S L -1.2893 Soluble
84 G L -1.7861 Soluble
85 T L -2.2265 Soluble
86 D L -2.6705 Soluble
87 F L 0.0000 Buried
88 T L -0.7272 Soluble
89 L L 0.0000 Buried
90 T L -0.5734 Soluble
91 I L 0.0000 Buried
92 S L -1.2828 Soluble
93 S L -1.0598 Soluble
94 L L 0.0000 Buried
95 Q L -0.8307 Soluble
96 P L -0.8385 Soluble
97 E L -1.8759 Soluble
98 D L 0.0000 Buried
99 F L -0.4288 Soluble
100 A L 0.0000 Buried
101 T L -0.8053 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L 0.9483 Aggregation-prone
108 Y L 0.9702 Aggregation-prone
109 T L 0.4836 Aggregation-prone
114 I L 0.6510 Aggregation-prone
115 P L -0.1451 Soluble
116 W L 0.0792 Aggregation-prone
117 T L -0.4678 Soluble
118 F L -0.3393 Soluble
119 G L 0.0000 Buried
120 G L -1.1604 Soluble
121 G L -0.9116 Soluble
122 T L 0.0000 Buried
123 K L -1.3604 Soluble
124 L L 0.0000 Buried
125 E L -0.7851 Soluble
126 I L -0.3187 Soluble
127 K L -1.2254 Soluble
128 R L -1.0990 Soluble
129 T L -0.1975 Soluble
130 V L 0.2739 Aggregation-prone
131 A L -0.0281 Soluble
132 A L -0.0983 Soluble
133 P L 0.0000 Buried
134 S L -0.1237 Soluble
135 V L 0.0000 Buried
136 F L -0.0022 Soluble
137 I L 0.0003 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5458 Soluble
140 P L -0.8710 Soluble
141 S L -1.6763 Soluble
142 D L -3.0332 Soluble
143 E L -3.1586 Soluble
144 Q L 0.0000 Buried
145 L L -2.2280 Soluble
146 K L -2.9250 Soluble
147 S L -1.8459 Soluble
148 G L -1.3103 Soluble
149 T L -1.1280 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7470 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7347 Soluble
162 R L -2.7532 Soluble
163 E L -3.1600 Soluble
164 A L -2.2627 Soluble
165 K L -2.1623 Soluble
166 V L -0.9326 Soluble
167 Q L -0.4513 Soluble
168 W L 0.0000 Buried
169 K L -0.4386 Soluble
170 V L 0.0000 Buried
171 D L -1.6926 Soluble
172 N L -1.2709 Soluble
173 A L -0.1966 Soluble
174 L L 0.7912 Aggregation-prone
175 Q L -0.0535 Soluble
176 S L -0.3576 Soluble
177 G L -0.9187 Soluble
178 N L -0.8427 Soluble
179 S L -1.1724 Soluble
180 Q L -1.5539 Soluble
181 E L -2.0245 Soluble
182 S L -0.9053 Soluble
183 V L -0.8675 Soluble
184 T L -1.1803 Soluble
185 E L -2.2735 Soluble
186 Q L -1.8291 Soluble
187 D L -1.8286 Soluble
188 S L -1.6314 Soluble
189 K L -2.2141 Soluble
190 D L -1.6085 Soluble
191 S L -1.6337 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7466 Soluble
199 L L 0.0000 Buried
200 T L -0.5011 Soluble
201 L L -0.7446 Soluble
202 S L -1.1389 Soluble
203 K L -2.0861 Soluble
204 A L -1.9128 Soluble
205 D L -2.7879 Soluble
206 Y L 0.0000 Buried
207 E L -3.0108 Soluble
208 K L -3.3889 Soluble
209 H L -2.9946 Soluble
210 K L -3.0576 Soluble
211 V L -1.6665 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8393 Soluble
216 V L 0.0000 Buried
217 T L -1.1498 Soluble
218 H L 0.0000 Buried
219 Q L -1.7451 Soluble
220 G L -0.4238 Soluble
221 L L -0.1668 Soluble
222 S L -0.4147 Soluble
223 S L -0.4020 Soluble
224 P L -0.5592 Soluble
225 V L -0.0148 Soluble
226 T L -0.4778 Soluble
227 K L -0.7392 Soluble
228 S L -0.6831 Soluble
229 F L -0.9991 Soluble
230 N L -2.1637 Soluble
231 R L -2.3847 Soluble
232 G L -1.6295 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6451 2.1629 CSV PDB
4.5 -0.69 2.1528 CSV PDB
5.0 -0.7413 2.1416 CSV PDB
5.5 -0.7895 2.1301 CSV PDB
6.0 -0.8242 2.118 CSV PDB
6.5 -0.8364 2.1048 CSV PDB
7.0 -0.8252 2.0908 CSV PDB
7.5 -0.7981 2.0765 CSV PDB
8.0 -0.7622 2.0623 CSV PDB
8.5 -0.7193 2.0483 CSV PDB
9.0 -0.6692 2.0339 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH