Amubarvimab / 8gx9

A4DAB014_8gx9_H_F | Source PDB: 8gx9 | Chains: H, F | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7046
Max Score
1.9283
Min Score
-3.7053
Total Score
-298.03
Score CDR
-0.1026
Score non-CDR
-0.7745

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGITVSSNYMNWVRQAPGKGLEWVSLIYSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYHCARDLVVYGMDVWGQGTTVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), F (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.7053
Maximum Score 1.9283
Average Score -0.7046
Total Score -298.0318

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 0.9472 Aggregation-prone
3 Q H -0.3428 Soluble
4 L H 0.0000 Buried
5 V H 0.3640 Aggregation-prone
6 E H -0.2192 Soluble
7 S H -0.4770 Soluble
8 G H -0.8724 Soluble
9 G H -0.5495 Soluble
11 G H -0.1316 Soluble
12 L H 0.2949 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.6895 Soluble
15 P H -1.9491 Soluble
16 G H -1.6084 Soluble
17 G H -1.1751 Soluble
18 S H -1.2580 Soluble
19 L H -0.8310 Soluble
20 R H -1.7598 Soluble
21 L H 0.0000 Buried
22 S H -0.4875 Soluble
23 C H 0.0000 Buried
24 A H -0.3606 Soluble
25 A H 0.0000 Buried
26 S H -0.1763 Soluble
27 G H -0.0628 Soluble
28 I H 0.0000 Buried
29 T H -0.8289 Soluble
30 V H 0.0000 Buried
35 S H -0.9682 Soluble
36 S H -0.5478 Soluble
37 N H -0.2278 Soluble
38 Y H 0.1540 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8163 Soluble
45 A H -1.2375 Soluble
46 P H -1.0265 Soluble
47 G H -1.4640 Soluble
48 K H -2.3865 Soluble
49 G H -1.6373 Soluble
50 L H 0.0000 Buried
51 E H -1.1031 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 L H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H -0.0254 Soluble
58 S H -0.4226 Soluble
59 G H -0.7059 Soluble
63 G H -0.7182 Soluble
64 S H -0.3035 Soluble
65 T H 0.0889 Aggregation-prone
66 Y H 0.1095 Aggregation-prone
67 Y H -0.5459 Soluble
68 A H -1.2522 Soluble
69 D H -2.3643 Soluble
70 S H -1.7635 Soluble
71 V H 0.0000 Buried
72 K H -2.5211 Soluble
74 G H -1.8053 Soluble
75 R H -1.4524 Soluble
76 F H 0.0000 Buried
77 T H -0.7960 Soluble
78 I H 0.0000 Buried
79 S H -0.5563 Soluble
80 R H -1.4538 Soluble
81 D H -2.2428 Soluble
82 N H -2.5676 Soluble
83 S H -2.0191 Soluble
84 K H -2.6419 Soluble
85 N H -1.9985 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6726 Soluble
89 L H 0.0000 Buried
90 Q H -1.1499 Soluble
91 M H 0.0000 Buried
92 N H -1.7038 Soluble
93 S H -1.5092 Soluble
94 L H 0.0000 Buried
95 R H -2.9549 Soluble
96 A H -2.0554 Soluble
97 E H -2.4135 Soluble
98 D H 0.0000 Buried
99 T H -0.8436 Soluble
100 A H 0.0000 Buried
101 V H 0.0315 Aggregation-prone
102 Y H 0.0000 Buried
103 H H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.2565 Soluble
107 D H 0.0000 Buried
108 L H 1.1609 Aggregation-prone
109 V H 1.3902 Aggregation-prone
110 V H 1.9283 Aggregation-prone
113 Y H 1.7333 Aggregation-prone
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.9400 Soluble
117 V H -0.4966 Soluble
118 W H -0.6529 Soluble
119 G H 0.0000 Buried
120 Q H -1.5043 Soluble
121 G H -0.7189 Soluble
122 T H -0.3606 Soluble
123 T H -0.1749 Soluble
124 V H 0.0000 Buried
125 T H -0.5435 Soluble
126 V H 0.0000 Buried
127 S H -1.0861 Soluble
128 S H -0.8983 Soluble
129 A H -0.5413 Soluble
130 S H -0.6819 Soluble
131 T H -0.8273 Soluble
132 K H -1.6303 Soluble
133 G H -1.7226 Soluble
134 P H 0.0000 Buried
135 S H -0.5215 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1475 Soluble
139 L H 0.0000 Buried
140 A H -0.6941 Soluble
141 P H -0.3371 Soluble
142 S H -0.2766 Soluble
143 G H -0.6262 Soluble
144 T H -0.4324 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.6057 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.9564 Soluble
157 E H -1.2694 Soluble
158 P H -1.1360 Soluble
159 V H -0.9619 Soluble
160 T H -0.7826 Soluble
161 V H -0.3074 Soluble
162 S H -0.4200 Soluble
163 W H 0.0000 Buried
164 N H -0.7360 Soluble
165 S H -0.6435 Soluble
166 G H -0.6039 Soluble
167 A H -0.2696 Soluble
168 L H -0.0385 Soluble
169 T H -0.2083 Soluble
170 S H -0.2227 Soluble
171 G H -0.2755 Soluble
172 V H 0.1151 Aggregation-prone
173 H H -0.0900 Soluble
174 T H 0.1080 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.1898 Soluble
177 A H 0.1705 Aggregation-prone
178 V H 0.2090 Aggregation-prone
179 L H 1.0168 Aggregation-prone
180 Q H 0.0719 Aggregation-prone
181 S H -0.1081 Soluble
182 S H -0.2169 Soluble
183 G H 0.0186 Aggregation-prone
184 L H -0.0659 Soluble
185 Y H 0.3046 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.1496 Aggregation-prone
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1442 Soluble
193 V H 0.0000 Buried
194 P H -0.4217 Soluble
195 S H -0.4433 Soluble
196 S H -0.4853 Soluble
197 S H -0.4426 Soluble
198 L H -0.4476 Soluble
199 G H -0.8322 Soluble
200 T H -0.6719 Soluble
201 Q H -1.2154 Soluble
202 T H -1.0672 Soluble
203 Y H 0.0000 Buried
204 I H -1.1065 Soluble
205 C H 0.0000 Buried
206 N H -1.3936 Soluble
207 V H 0.0000 Buried
208 N H -2.4009 Soluble
209 H H 0.0000 Buried
210 K H -2.9077 Soluble
211 P H -1.8518 Soluble
212 S H -2.0269 Soluble
213 N H -2.6671 Soluble
214 T H -2.2606 Soluble
215 K H -2.8128 Soluble
216 V H -1.5865 Soluble
217 D H -2.1546 Soluble
218 K H -1.9444 Soluble
219 K H -2.3092 Soluble
220 V H 0.0000 Buried
221 E H -2.3094 Soluble
1 E F -1.5181 Soluble
2 I F -0.1286 Soluble
3 V F 0.7944 Aggregation-prone
4 L F 0.0000 Buried
5 T F -0.9290 Soluble
6 Q F 0.0000 Buried
7 S F -0.9230 Soluble
8 P F -0.4792 Soluble
9 G F -0.8144 Soluble
10 T F -0.4817 Soluble
11 L F -0.2007 Soluble
12 S F -0.4883 Soluble
13 L F -0.9937 Soluble
14 S F -1.4520 Soluble
15 P F -1.8871 Soluble
16 G F -2.1133 Soluble
17 E F -2.8815 Soluble
18 R F -3.2254 Soluble
19 A F 0.0000 Buried
20 T F -0.5348 Soluble
21 L F 0.0000 Buried
22 S F -0.9360 Soluble
23 C F 0.0000 Buried
24 R F -2.2990 Soluble
25 A F 0.0000 Buried
26 S F -0.9091 Soluble
27 Q F -1.4834 Soluble
28 S F -0.9316 Soluble
29 V F -0.5659 Soluble
30 S F -0.2923 Soluble
36 S F -0.3304 Soluble
37 S F 0.4111 Aggregation-prone
38 Y F 0.9189 Aggregation-prone
39 L F 0.0000 Buried
40 A F 0.0000 Buried
41 W F 0.0000 Buried
42 Y F 0.0000 Buried
43 Q F 0.0000 Buried
44 Q F -1.1913 Soluble
45 K F -1.5278 Soluble
46 P F -1.2750 Soluble
47 G F -1.5023 Soluble
48 Q F -2.1828 Soluble
49 A F -1.5546 Soluble
50 P F 0.0000 Buried
51 R F -1.7621 Soluble
52 L F 0.0000 Buried
53 L F 0.0000 Buried
54 I F 0.0000 Buried
55 Y F 0.3187 Aggregation-prone
56 G F 0.3885 Aggregation-prone
57 A F -0.2183 Soluble
65 S F -0.7306 Soluble
66 S F -0.7657 Soluble
67 R F -1.9015 Soluble
68 A F -0.9669 Soluble
69 T F -0.7460 Soluble
70 G F -0.8637 Soluble
71 I F -1.1101 Soluble
72 P F -1.3693 Soluble
74 D F -2.4356 Soluble
75 R F -2.0817 Soluble
76 F F 0.0000 Buried
77 S F -0.9929 Soluble
78 G F -0.5628 Soluble
79 S F -0.6025 Soluble
80 G F -0.9169 Soluble
83 S F -0.9017 Soluble
84 G F -1.1738 Soluble
85 T F -1.7173 Soluble
86 D F -2.2950 Soluble
87 F F 0.0000 Buried
88 T F -0.7940 Soluble
89 L F 0.0000 Buried
90 T F -0.7745 Soluble
91 I F 0.0000 Buried
92 S F -2.3339 Soluble
93 R F -3.3713 Soluble
94 L F 0.0000 Buried
95 E F -2.1927 Soluble
96 P F -1.3356 Soluble
97 E F -1.9984 Soluble
98 D F 0.0000 Buried
99 F F -0.6474 Soluble
100 A F 0.0000 Buried
101 V F -0.5294 Soluble
102 Y F 0.0000 Buried
103 Y F 0.0000 Buried
104 C F 0.0000 Buried
105 Q F 0.0000 Buried
106 Q F 0.0000 Buried
107 Y F 0.0000 Buried
108 G F -0.7381 Soluble
114 S F -0.5571 Soluble
115 S F -0.2792 Soluble
116 P F 0.0000 Buried
117 T F -0.0570 Soluble
118 F F 0.0000 Buried
119 G F 0.0000 Buried
120 Q F -1.5862 Soluble
121 G F 0.0000 Buried
122 T F 0.0000 Buried
123 K F -1.1035 Soluble
124 L F 0.0000 Buried
125 E F 0.0000 Buried
126 I F -0.7714 Soluble
127 K F -1.5832 Soluble
128 R F -2.0485 Soluble
129 T F -0.4101 Soluble
130 V F 0.9247 Aggregation-prone
131 A F 0.2086 Aggregation-prone
132 A F 0.0480 Aggregation-prone
133 P F -0.0699 Soluble
134 S F -0.1038 Soluble
135 V F 0.0000 Buried
136 F F 0.1363 Aggregation-prone
137 I F 0.0791 Aggregation-prone
138 F F 0.0000 Buried
139 P F -0.5192 Soluble
140 P F 0.0000 Buried
141 S F -1.7277 Soluble
142 D F -3.0600 Soluble
143 E F -3.2459 Soluble
144 Q F 0.0000 Buried
145 L F -2.3816 Soluble
146 K F -2.8877 Soluble
147 S F -1.8217 Soluble
148 G F -1.3923 Soluble
149 T F -1.0035 Soluble
150 A F 0.0000 Buried
151 S F 0.0000 Buried
152 V F 0.0000 Buried
153 V F 0.0000 Buried
154 C F 0.0000 Buried
155 L F 0.0000 Buried
156 L F 0.0000 Buried
157 N F 0.0000 Buried
158 N F -0.9006 Soluble
159 F F 0.0000 Buried
160 Y F 0.0000 Buried
161 P F -1.4895 Soluble
162 R F -2.4753 Soluble
163 E F -2.9037 Soluble
164 A F -2.1754 Soluble
165 K F -2.2070 Soluble
166 V F -1.2137 Soluble
167 Q F -0.5276 Soluble
168 W F 0.0000 Buried
169 K F -0.6602 Soluble
170 V F 0.0000 Buried
171 D F -2.0089 Soluble
172 N F -1.3811 Soluble
173 A F -0.3354 Soluble
174 L F 0.7127 Aggregation-prone
175 Q F -0.2204 Soluble
176 S F -0.3711 Soluble
177 G F -0.9599 Soluble
178 N F -0.8775 Soluble
179 S F -1.3950 Soluble
180 Q F -2.0655 Soluble
181 E F -2.2012 Soluble
182 S F -0.7788 Soluble
183 V F -0.3546 Soluble
184 T F 0.0000 Buried
185 E F -2.0922 Soluble
186 Q F -1.7964 Soluble
187 D F -2.0367 Soluble
188 S F -1.7829 Soluble
189 K F -2.3877 Soluble
190 D F -2.1927 Soluble
191 S F 0.0000 Buried
192 T F 0.0000 Buried
193 Y F 0.0000 Buried
194 S F 0.0000 Buried
195 L F 0.0000 Buried
196 S F 0.0000 Buried
197 S F 0.0000 Buried
198 T F -0.7951 Soluble
199 L F 0.0000 Buried
200 T F -0.4669 Soluble
201 L F -0.5803 Soluble
202 S F -1.2341 Soluble
203 K F -2.1641 Soluble
204 A F -1.9130 Soluble
205 D F -2.4191 Soluble
206 Y F 0.0000 Buried
207 E F -3.7011 Soluble
208 K F -3.7053 Soluble
209 H F -3.1033 Soluble
210 K F -3.2342 Soluble
211 V F -1.4366 Soluble
212 Y F 0.0000 Buried
213 A F 0.0000 Buried
214 C F 0.0000 Buried
215 E F -0.8067 Soluble
216 V F 0.0000 Buried
217 T F -1.1309 Soluble
218 H F 0.0000 Buried
219 Q F -1.3793 Soluble
220 G F -0.2965 Soluble
221 L F -0.1912 Soluble
222 S F -0.4553 Soluble
223 S F -0.3790 Soluble
224 P F -0.5036 Soluble
225 V F 0.0933 Aggregation-prone
226 T F -0.4133 Soluble
227 K F -0.6952 Soluble
228 S F -0.5175 Soluble
229 F F -0.8674 Soluble
230 N F -1.9102 Soluble
231 R F -2.5034 Soluble
232 G F -2.0454 Soluble
233 E F -2.1598 Soluble
234 C F -0.4260 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7353 1.8142 CSV PDB
4.5 -0.7791 1.7723 CSV PDB
5.0 -0.8306 1.729 CSV PDB
5.5 -0.8807 1.7122 CSV PDB
6.0 -0.9185 1.7122 CSV PDB
6.5 -0.9349 1.7122 CSV PDB
7.0 -0.9288 1.7122 CSV PDB
7.5 -0.9073 1.712 CSV PDB
8.0 -0.8768 1.7116 CSV PDB
8.5 -0.8394 1.7104 CSV PDB
9.0 -0.7957 1.7065 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH