Dostarlimab / 7wsl

A4DAB090_7wsl_H_L | Source PDB: 7wsl | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6285
Max Score
1.5642
Min Score
-3.4708
Total Score
-264.59
Score CDR
-0.1823
Score non-CDR
-0.6792

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYDMSWVRQAPGKGLEWVSTISGGGSYTYYQDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCASPYYAMDYWGQGTTVTVSSASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQLTQSPSFLSAYVGDRVTITCKASQDVGTAVAWYQQKPGKAPKLLIYWASTLHTGVPSRFSGSGSGTEFTLTISSLQPEDFATYYCQHYSSYPWTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4708
Maximum Score 1.5642
Average Score -0.6285
Total Score -264.5874

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0785 Soluble
2 V H -1.1558 Soluble
3 Q H -1.0259 Soluble
4 L H 0.0000 Buried
5 L H 0.9999 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4186 Soluble
8 G H -0.7968 Soluble
9 G H -0.4237 Soluble
11 G H 0.0879 Aggregation-prone
12 L H 0.6236 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.4952 Soluble
15 P H -1.6610 Soluble
16 G H -1.4550 Soluble
17 G H -1.0450 Soluble
18 S H -1.1401 Soluble
19 L H -0.8338 Soluble
20 R H -1.5780 Soluble
21 L H 0.0000 Buried
22 S H -0.2641 Soluble
23 C H 0.0000 Buried
24 A H -0.1207 Soluble
25 A H 0.0000 Buried
26 S H -0.8022 Soluble
27 G H -1.2220 Soluble
28 F H -0.3750 Soluble
29 T H -0.1444 Soluble
30 F H 0.0000 Buried
35 S H -0.8912 Soluble
36 S H -0.2867 Soluble
37 Y H 0.2708 Aggregation-prone
38 D H -0.1431 Soluble
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7945 Soluble
45 A H -1.2400 Soluble
46 P H -1.1926 Soluble
47 G H -1.4549 Soluble
48 K H -2.3039 Soluble
49 G H -1.4979 Soluble
50 L H 0.0000 Buried
51 E H -1.0007 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 T H 0.0000 Buried
56 I H 0.0000 Buried
57 S H -0.0000 Buried
58 G H -0.4611 Soluble
59 G H -0.6858 Soluble
62 G H -0.4122 Soluble
63 S H 0.1643 Aggregation-prone
64 Y H 1.1668 Aggregation-prone
65 T H 0.9243 Aggregation-prone
66 Y H 0.8991 Aggregation-prone
67 Y H -0.4006 Soluble
68 Q H -1.4101 Soluble
69 D H -2.5933 Soluble
70 S H -1.6468 Soluble
71 V H 0.0000 Buried
72 K H -2.4469 Soluble
74 G H -1.6441 Soluble
75 R H 0.0000 Buried
76 F H 0.0000 Buried
77 T H -0.5822 Soluble
78 I H 0.0000 Buried
79 S H -0.2830 Soluble
80 R H -1.1421 Soluble
81 D H -1.7974 Soluble
82 N H -1.8975 Soluble
83 S H -1.6762 Soluble
84 K H -2.4568 Soluble
85 N H -1.8596 Soluble
86 T H -1.0461 Soluble
87 L H 0.0000 Buried
88 Y H -0.3716 Soluble
89 L H 0.0000 Buried
90 Q H -0.9078 Soluble
91 M H 0.0000 Buried
92 N H -1.2765 Soluble
93 S H -1.2042 Soluble
94 L H 0.0000 Buried
95 R H -2.2847 Soluble
96 A H -1.7455 Soluble
97 E H -2.2366 Soluble
98 D H 0.0000 Buried
99 T H -0.7395 Soluble
100 A H 0.0000 Buried
101 V H 0.0910 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H 0.0000 Buried
107 P H 0.0000 Buried
108 Y H 1.5642 Aggregation-prone
109 Y H 1.2403 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H 0.1724 Aggregation-prone
117 Y H 0.4047 Aggregation-prone
118 W H -0.1777 Soluble
119 G H 0.0000 Buried
120 Q H -1.3284 Soluble
121 G H 0.0000 Buried
122 T H -0.1829 Soluble
123 T H -0.0796 Soluble
124 V H 0.0000 Buried
125 T H -0.2544 Soluble
126 V H 0.0000 Buried
127 S H -0.8563 Soluble
128 S H -0.6656 Soluble
129 A H -0.4427 Soluble
130 S H -0.6332 Soluble
131 T H -0.7707 Soluble
132 K H -1.2290 Soluble
133 G H -1.3743 Soluble
134 P H 0.0000 Buried
135 S H -0.4121 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.1710 Soluble
139 L H 0.0000 Buried
140 A H -0.8371 Soluble
141 P H -0.4904 Soluble
142 G H -0.6561 Soluble
143 T H -0.5102 Soluble
144 A H -0.2203 Soluble
145 A H 0.0000 Buried
146 L H 0.0000 Buried
147 G H 0.0000 Buried
148 C H 0.0000 Buried
149 L H 0.0000 Buried
150 V H 0.0000 Buried
151 K H 0.0000 Buried
152 D H -0.4334 Soluble
153 Y H 0.0000 Buried
154 F H 0.0000 Buried
155 P H -0.9944 Soluble
156 E H -1.3765 Soluble
157 P H -1.2791 Soluble
158 V H -0.9427 Soluble
159 T H -0.7317 Soluble
160 V H -0.2270 Soluble
161 S H -0.4494 Soluble
162 W H 0.0000 Buried
163 N H -0.8901 Soluble
164 S H -0.7117 Soluble
165 G H -0.5934 Soluble
166 A H -0.2891 Soluble
167 L H -0.0708 Soluble
168 T H -0.2154 Soluble
169 S H -0.2111 Soluble
170 G H -0.2679 Soluble
171 V H 0.1182 Aggregation-prone
172 H H -0.3128 Soluble
173 T H 0.0859 Aggregation-prone
174 F H 0.0000 Buried
175 P H -0.1762 Soluble
176 A H 0.1953 Aggregation-prone
177 V H 0.6312 Aggregation-prone
178 L H 1.1717 Aggregation-prone
179 Q H 0.3182 Aggregation-prone
180 S H -0.0391 Soluble
181 S H -0.2113 Soluble
182 G H 0.0365 Aggregation-prone
183 L H -0.0714 Soluble
184 Y H 0.0000 Buried
185 S H 0.0000 Buried
186 L H 0.2133 Aggregation-prone
187 S H 0.0000 Buried
188 S H 0.0000 Buried
189 V H 0.0000 Buried
190 V H 0.0000 Buried
191 T H -0.1611 Soluble
192 V H 0.0000 Buried
193 P H -0.4786 Soluble
194 S H -0.5312 Soluble
195 S H -0.6189 Soluble
196 S H 0.0000 Buried
197 L H -0.7652 Soluble
198 G H -0.9528 Soluble
199 T H -0.7749 Soluble
200 Q H -1.3599 Soluble
201 T H -1.2216 Soluble
202 Y H 0.0000 Buried
203 I H -1.4295 Soluble
204 C H 0.0000 Buried
205 N H -1.5742 Soluble
206 V H 0.0000 Buried
207 N H -2.2282 Soluble
208 H H 0.0000 Buried
209 K H -2.9309 Soluble
210 P H -1.7885 Soluble
211 S H -1.8888 Soluble
212 N H -2.6158 Soluble
213 T H -2.3552 Soluble
214 K H -2.8405 Soluble
215 V H -1.7288 Soluble
216 D H -2.6605 Soluble
217 K H -2.1729 Soluble
218 K H -2.6711 Soluble
219 V H 0.0000 Buried
220 E H -2.5032 Soluble
221 P H -1.1568 Soluble
1 D L -2.4522 Soluble
2 I L 0.0000 Buried
3 Q L -2.2946 Soluble
4 L L 0.0000 Buried
5 T L -1.3868 Soluble
6 Q L 0.0000 Buried
7 S L -0.3819 Soluble
8 P L 0.0356 Aggregation-prone
9 S L 0.1464 Aggregation-prone
10 F L 1.0389 Aggregation-prone
11 L L 0.6548 Aggregation-prone
12 S L 0.2412 Aggregation-prone
13 A L 0.0000 Buried
14 Y L 1.0476 Aggregation-prone
15 V L 1.2191 Aggregation-prone
16 G L -0.1929 Soluble
17 D L -1.0092 Soluble
18 R L -2.0094 Soluble
19 V L 0.0000 Buried
20 T L -0.6015 Soluble
21 I L 0.0000 Buried
22 T L -0.9474 Soluble
23 C L 0.0000 Buried
24 K L -2.9864 Soluble
25 A L 0.0000 Buried
26 S L -2.4413 Soluble
27 Q L -3.0967 Soluble
28 D L -3.0318 Soluble
29 V L 0.0000 Buried
36 G L -1.3211 Soluble
37 T L -0.6066 Soluble
38 A L 0.0000 Buried
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6995 Soluble
46 P L -1.4681 Soluble
47 G L -1.6664 Soluble
48 K L -2.5783 Soluble
49 A L -1.5309 Soluble
50 P L 0.0000 Buried
51 K L -1.3121 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.5778 Aggregation-prone
56 W L 0.6311 Aggregation-prone
57 A L 0.0000 Buried
65 S L -0.0611 Soluble
66 T L 0.2959 Aggregation-prone
67 L L 0.4352 Aggregation-prone
68 H L -0.1210 Soluble
69 T L -0.2583 Soluble
70 G L -0.4226 Soluble
71 V L -0.2781 Soluble
72 P L -0.2931 Soluble
74 S L -0.4019 Soluble
75 R L -0.7428 Soluble
76 F L 0.0000 Buried
77 S L -0.3316 Soluble
78 G L -0.1628 Soluble
79 S L -0.6632 Soluble
80 G L -1.1433 Soluble
83 S L -1.2368 Soluble
84 G L -2.0247 Soluble
85 T L -2.5932 Soluble
86 E L -3.0350 Soluble
87 F L 0.0000 Buried
88 T L -0.8838 Soluble
89 L L 0.0000 Buried
90 T L -0.6377 Soluble
91 I L 0.0000 Buried
92 S L -1.2770 Soluble
93 S L -0.9268 Soluble
94 L L 0.0000 Buried
95 Q L -0.5532 Soluble
96 P L -0.6088 Soluble
97 E L -1.8478 Soluble
98 D L 0.0000 Buried
99 F L -0.1758 Soluble
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 H L 0.0000 Buried
107 Y L 0.2605 Aggregation-prone
108 S L -0.2630 Soluble
109 S L -0.2420 Soluble
114 Y L -0.0146 Soluble
115 P L -0.6113 Soluble
116 W L 0.0000 Buried
117 T L -0.7666 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 Q L -1.4535 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.3186 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L 0.4809 Aggregation-prone
127 K L -0.1103 Soluble
128 R L -0.5181 Soluble
129 T L -0.0021 Soluble
130 V L 0.5644 Aggregation-prone
131 A L 0.0918 Aggregation-prone
132 A L -0.1432 Soluble
133 P L 0.0000 Buried
134 S L -0.2028 Soluble
135 V L 0.0000 Buried
136 F L 0.1251 Aggregation-prone
137 I L 0.1267 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.5747 Soluble
140 P L -0.8052 Soluble
141 S L -1.6026 Soluble
142 D L -2.8785 Soluble
143 E L -2.8286 Soluble
144 Q L 0.0000 Buried
145 L L -2.2789 Soluble
146 K L -2.8209 Soluble
147 S L -1.7834 Soluble
148 G L -1.4437 Soluble
149 T L -1.0973 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9214 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -0.9316 Soluble
162 R L -1.4529 Soluble
163 E L -2.5514 Soluble
164 A L -1.9790 Soluble
165 K L -2.3459 Soluble
166 V L -1.2195 Soluble
167 Q L -0.8352 Soluble
168 W L 0.0000 Buried
169 K L -0.7671 Soluble
170 V L 0.0000 Buried
171 D L -1.7927 Soluble
172 N L -1.3948 Soluble
173 A L -0.3093 Soluble
174 L L 0.5831 Aggregation-prone
175 Q L -0.3875 Soluble
176 S L -0.6852 Soluble
177 G L -1.2669 Soluble
178 N L -1.5611 Soluble
179 S L -1.4439 Soluble
180 Q L -1.4112 Soluble
181 E L -1.5766 Soluble
182 S L -0.5164 Soluble
183 V L -0.2691 Soluble
184 T L -0.9235 Soluble
185 E L -2.0663 Soluble
186 Q L -1.7328 Soluble
187 D L -2.1129 Soluble
188 S L -1.7853 Soluble
189 K L -2.3643 Soluble
190 D L -1.8840 Soluble
191 S L -1.6761 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L -0.2949 Soluble
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6950 Soluble
199 L L 0.0000 Buried
200 T L -0.6639 Soluble
201 L L -0.8823 Soluble
202 S L -1.1444 Soluble
203 K L -2.1399 Soluble
204 A L -1.9334 Soluble
205 D L -2.7591 Soluble
206 Y L 0.0000 Buried
207 E L -3.3932 Soluble
208 K L -3.4708 Soluble
209 H L -2.9227 Soluble
210 K L -3.0587 Soluble
211 V L -1.6962 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8918 Soluble
216 V L 0.0000 Buried
217 T L -1.2333 Soluble
218 H L 0.0000 Buried
219 Q L -1.6005 Soluble
220 G L -0.4113 Soluble
221 L L -0.1538 Soluble
222 S L -0.4117 Soluble
223 S L -0.4214 Soluble
224 P L -0.6298 Soluble
225 V L -0.0211 Soluble
226 T L -0.5525 Soluble
227 K L -0.7706 Soluble
228 S L -0.7102 Soluble
229 F L -0.9611 Soluble
230 N L -2.0882 Soluble
231 R L -2.3030 Soluble
232 G L -1.4439 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5497 2.1693 CSV PDB
4.5 -0.5951 2.1699 CSV PDB
5.0 -0.6478 2.1721 CSV PDB
5.5 -0.698 2.1785 CSV PDB
6.0 -0.7352 2.1959 CSV PDB
6.5 -0.7498 2.2347 CSV PDB
7.0 -0.7407 2.2991 CSV PDB
7.5 -0.7157 2.3812 CSV PDB
8.0 -0.6822 2.4712 CSV PDB
8.5 -0.6423 2.5641 CSV PDB
9.0 -0.5955 2.6576 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH